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玫瑰黄酮醇合成酶FLS基因的克隆及生物信息学分析

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山东林业科技 2018年第3期48卷 1-6页

作者：李忠健张鹏远靳金粉赵兰勇徐宗大山东农业大学林学院山东泰安271018 山东省林木种苗和花卉站山东济南250000

本研究目的在于克隆玫瑰FLS基因,并对其进行生物信息学分析,为今后玫瑰新品种培育奠定基础。以玫瑰野生类型‘珲春’(Rosa rugosa‘Hunchun’)为试材,采用RT-PCR和RACE方法从花瓣中获得了玫瑰黄酮醇合成酶(Flavonol synthase,FLS)基因的... 详细信息

本研究目的在于克隆玫瑰FLS基因,并对其进行生物信息学分析,为今后玫瑰新品种培育奠定基础。以玫瑰野生类型‘珲春’(Rosa rugosa‘Hunchun’)为试材,采用RT-PCR和RACE方法从花瓣中获得了玫瑰黄酮醇合成酶(Flavonol synthase,FLS)基因的cDNA全长,命名为RrFLS。经生物信息学分析,该基因全长1287bp,开放阅读框1005bp,编码335个氨基酸,其蛋白分子式为C_(1731)H_(2681)N_(443)O_(502)S_9,相对分子质量为38018.54Da,pI=5.85,该蛋白不稳定系数为34.10,属于稳定蛋白,未检测到信号肽及剪切位点。系统进化树分析表明,玫瑰FLS与同科植物亲缘关系较近,其中与桃、蔷薇、草莓亲缘关系最近,与其他植物亲缘关系相对较远。

关键词：玫瑰黄酮醇合成酶基因克隆生物信息学

A Prognostic Nomogram with High Accuracy Based on 2D-SWE in Patients with Acute-on-chronic Liver Failure

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Journal of Clinical and Translational Hepatology 2022年第5期10卷 803-813页

作者： Lili Wu Jieyang jin Taicheng Zhou Yuankai Wu Xinhua Li Xiangyong Li Jie Zeng jinfen Wang Jie Ren Yutian Chong Rongqin Zheng Department of Medical Ultrasonics Third Affiliated Hospital of Sun Yat-Sen UniversityGuangdong Key Laboratory of Liver Disease ResearchGuangzhouGuangdongChina Department of Gastroenterological Surgery and Hernia Center Sixth Affiliated Hospital of Sun Yat-Sen UniversityGuangdong Institute of GastroenterologyGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor DiseasesSupported by National Key Clinical DisciplineGuangzhouGuangdongChina Department of Infectious Diseases Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouGuangdongChina

Background and Aims:Acute-on-chronic liver failure(ACLF)is associated with very high *** prediction of prognosis is critical in navigating optimal treatment decisions to improve patient *** study was aimed to develop a new nomogram integrating two-dimensional shear wave elastography(2D-SWE)values with other independent prognostic factors to improve the precision of predicting ACLF patient ***:A total of 449 consecutive patients with ACLF were recruited and randomly allocated to a training cohort(n=315)or a test cohort(n=134).2D-SWE values,conventional ultrasound features,laboratory tests,and other clinical characteristics were included in univariate and multivariate *** with prognostic value were then used to construct a novel prognostic *** operating curves(ROCs)were generated to evaluate and compare the performance of the novel and published models including the Model for EndStage Liver Disease(MELD),MELD combined with sodium(MELD-Na),and jin’s *** model was validated in a prospective cohort(n=102).Results:A ACLF prognostic nomogram was developed with independent prognostic factors,including 2D-SWE,age,total bilirubin(TB),neutrophils(Neu),and the international normalized ratio(INR).The area under the ROC curve(AUC)was 0.849 for the new model in the training cohort and 0.861 in the prospective validation cohort,which were significantly greater than those for MELD(0.758),MELD-Na(0.750),and jin’s model(0.777,all p<0.05).Calibration curve analysis revealed good agreement between the predicted and observed *** new nomogram had superior overall net benefit and clinical ***:We established and validated a 2D-SWE-based noninvasive nomogram to predict the prognosis of ACLF patients that was more accurate than other prognostic models.

关键词： Acute-on-chronic liver failure Two-dimensional shear wave elastography Hepatitis B virus Prognosis

Quantitative evaluation of CA4P early efficacy in tumor model with dynamic contrast-enhanced ultrasound

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Quantitative evaluation of CA4P early efficacy in tumor mode...

中国超声医学工程学会第十四届全国超声心动图学术会议

作者： Qiaofeng jin Yihan Chen Pingyu Zhang jinfen Liu jing Wang Yali Yang Qing Lv Mingxing Xie Department of Ultrasound Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Hubei Province Key Laboratory of Molecular Imaging

Objective:This study was to quantify both central and peripheral tumor perfusion with contrast-enhanced ultrasound(CEUS) in the evaluation of early tumor response to anti-vascular *** and Method:44 mice bearing with colon cancer were administrated with Combretastatin A4 phosphate(CA4 P) at 100 mg/*** was performed on treated groups(at 2,12,48 hours after the treatment) and untreated group(Oh).Time-intensity curves(TICs) were derived from regions of interest(ROI) in center(ROI) and peripheral(ROI) region of each tumor CEUS *** perfusion parameters including maximum intensity(IMAX),time to peak(TTP) and quality of fit(QOF)were calculated from TIC and normalized with respect to perfusion of adjacent *** analysis was conducted to evaluate tumor necrosis area%,microvascular density(MVD) and tumor ***:Significant decrease of IMAX in ROI was noticed from 2 hours after CA4 P therapy(P<0.01) and increase at 48 hours,but IMAX in ROI decreased significantly only at 12 hours(P<0.01).There were significant differences of TTP between 2 hours and other time points in both ROIcenter and ROIperiphery(P<0.01).After CA4 P treatment,vessel density decreased in the center of tumor from 2 hours,but not in the tumor *** decrease of tumor perfusion(Hoechst 33342 staining) and vascular perfusion(FITC-lectin staining) were seen at 2,12 and 48 hours after CA4 P treatment The IMAX were statistically positive correlated with Hoechst 33342 staining(r=0.77,P<0.001),compared with weaker correlation with MVD(r= 0.67,P<0.001)Conclusion:CEUS patterns and parameters are more closely correlated with tumor perfusion *** CEUS for quantification of tumor perfusion could be used for early detection of cancer response to anti-vascular treatment

关键词： dynamic contrast-enhanced ultrasound antiangiogenic treatments,perfusion parameters combretastatin A4 phosphate vascular disrupting agent

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猪传染性萎缩性鼻炎症状及防控

畜牧兽医科学（电子版） 2021年第2期 68-69页

作者：晋凤菊马金芬云南省曲靖市罗平县九龙街道畜牧兽医站曲靖655800 云南省曲靖市师宗县畜牧兽医局曲靖655700

猪传染性萎缩性鼻炎是由支气管败血波氏杆菌或多杀性巴氏杆菌感染引发,多发生于猪群,虽然不会造成大量死亡病例,但是会对发病群体正常发育产生严重威胁,普遍表现为发育迟缓,甚至停止生长发育,使养殖场成本投入进一步增加,严重影响养殖... 详细信息

猪传染性萎缩性鼻炎是由支气管败血波氏杆菌或多杀性巴氏杆菌感染引发,多发生于猪群,虽然不会造成大量死亡病例,但是会对发病群体正常发育产生严重威胁,普遍表现为发育迟缓,甚至停止生长发育,使养殖场成本投入进一步增加,严重影响养殖场经济效益。此外,猪传染性萎缩性鼻炎发生后,还会严重造成猪群出现严重的免疫抑制,给多种病原混合发病提供条件,会进一步增加疾病诊断难度。该文主要分析猪传染性萎缩性鼻炎的临床症状、病理变化、诊断方法、防治措施。

关键词：猪传染性萎缩性鼻炎临床症状防控措施

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青岛滨海高速公路路面径流金属污染研究

海洋湖沼通报 2012年第2期 123-128页

作者：李宝洁赵帅于俊英郭少平潘进芬李瑾中国海洋大学海洋环境与生态教育部重点实验室山东青岛266100 中国海洋大学环境科学与工程学院山东青岛266100

滨海高速公路径流作为沿海城市金属污染源之一受到国内外关注。青岛市青银高速公路路面径流金属Cr、Cu、Zn、Hg及Pb分析表明,青岛高速公路路面径流水质较好,基本符合国家V类水质标准。初期径流水质中污染物浓度较高,随着降雨冲刷强度突... 详细信息

滨海高速公路径流作为沿海城市金属污染源之一受到国内外关注。青岛市青银高速公路路面径流金属Cr、Cu、Zn、Hg及Pb分析表明,青岛高速公路路面径流水质较好,基本符合国家V类水质标准。初期径流水质中污染物浓度较高,随着降雨冲刷强度突然增大,出现"二次冲刷"现象。结合主成分分析推知径流中Cr、Cu、Zn及Cd主要来源于大气沉降,而Hg与Pb主要来源于大气的道路交通污染。

关键词：路面径流水质相关性流出规律

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桂花镇林业生态助推脱贫攻坚的思考

内蒙古林业调查设计 2019年第2期42卷 74-76页

作者：李金芬陆琳金钱荣桂花镇林业站云南大姚675409 云南森林自然中心云南昆明650224

根据国家和省州县扶贫战略总体部署,结合全镇林业生态建设和林业产业发展实际,坚持"创新、协调、绿色、开放、共享"和"生态建设产业化,产业发展生态化"的发展理念,按照科学治贫、精准扶贫和有效脱贫的要求,采取一系列有效措施,有效推进... 详细信息

根据国家和省州县扶贫战略总体部署,结合全镇林业生态建设和林业产业发展实际,坚持"创新、协调、绿色、开放、共享"和"生态建设产业化,产业发展生态化"的发展理念,按照科学治贫、精准扶贫和有效脱贫的要求,采取一系列有效措施,有效推进全镇林业精准扶贫工作的开展。

关键词：林业生态建设对策措施精准脱贫

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羊传染性胸膜肺炎流行特点及防治

畜牧兽医科学（电子版） 2021年第20期 64-65页

作者：马金芬晋凤菊张锦坤云南省曲靖市师宗县畜牧兽医局云南曲靖655700 云南省曲靖市罗平县九龙街道畜牧兽医站云南曲靖655800

羊传染性胸膜肺炎是由肺炎支原体感染引发的一种接触性疾病,该种疾病在羊群中传播速度较快,感染范围较广,不同年龄品种的羊都会受到病原感染,常呈地方性流行。新发病的养殖场发病较急,发病过程较短,老疫区常呈慢性感染、周期性感染。传... 详细信息

羊传染性胸膜肺炎是由肺炎支原体感染引发的一种接触性疾病,该种疾病在羊群中传播速度较快,感染范围较广,不同年龄品种的羊都会受到病原感染,常呈地方性流行。新发病的养殖场发病较急,发病过程较短,老疫区常呈慢性感染、周期性感染。传染性胸膜肺炎一旦发生流行,将需要消耗较多的时间和精力用于羊群净化处理,否则隐性感染羊会频繁和健康羊接触,造成发病率较高。该文探讨羊传染性胸膜肺炎的流行特点和防治措施。

关键词：羊传染性胸膜肺炎流行特点防治措施