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Correlation of aquaporin-4 expression to blood-brain barrier permeability in rats with focal cerebral ischemia

Neural Regeneration Research 2008年第6期3卷 629-633页

作者： Pengcheng xu Haorong Feng jinbu xu Yongping Wu Jiangsu Province Key Laboratory of Anesthesiology Xuzhou Medical College Xuzhou 221002 Jiangsu Province China Department of Anesthesiology the 98 Hospital of Chinese PLA Huzhou 313000 Zhejiang Province China

BACKGROUND： Ischemic cerebrovascular disease causes injury to the blood-brain barrier. The occurrence of brain edema is associated with aquaporin expression following cerebral ischemia/reperfusion. OBJECTIVE： To analyze the correlation of aquaporin-4 expression to brain edema and blood-brain barrier permeability in brain tissues of rat models of ischemia/reperfusion. DESIGN, TIME AND SETTING： The randomized control experiment was performed at the Jiangsu Province Key Laboratory of Anesthesiology, xuzhou Medical College, China from December 2006 to October 2007. MATERIALS： A total of 112 adult, male, Sprague-Dawley rats, weighing 220-250 g, were used to establish rat models of middle cerebral artery occlusion and reperfusion by the suture method. Rabbit anti-aquaporin-4 （Santa Cruz, USA） and Evans blue （Sigma, USA） were used to analyze the tissue. METHODS： The rats were randomized into sham-operated （n = 16） and ischemia/reperfusion （n = 96） groups. There were 6 time points in the ischemia/reperfusion group, comprising 4, 6, 12, 24, 48, and 72 hours after reperfusion, with 16 rats for each time point. Rat models in the sham-operated group at 4 hours after surgery and rat models in the ischemia/reperfusion group at different time points were equally and randomly assigned into 4 different subgroups. MAIN OUTCOME MEASURES： Brain water content on the ischemic side and the control side was measured using the dry-wet weight method. Blood-brain barrier function was determined by Evans Blue. Aquaporin-4 expression surrounding the ischemic focus, as well as the correlation of aquaporin-4 expression with brain water content and Evans blue staining, were measured using immunohistochemistry and Western blot analysis. RESULTS： Brain water content on the ischemic side significantly increased at 12 hours after reperfusion, reached a peak at 48 hours, and was still high at 72 hours. Brain water content was greater on the ischemic hemispheres, compared with the control hemispheres at

关键词： aquaporin-4 blood-brain barrier Evans blue brain edema

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肠道菌群通过戊酸调节脂多糖诱导的急性肺损伤

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中国微生态学杂志 2024年第10期36卷 1163-1169页

作者：邵兰徐进步佛山市妇幼保健院重症医学科广东528000

目的观察广谱抗生素诱导的肠道菌群改变对脂多糖(lipopolysaccharide,LPS)诱导的急性肺损伤(acute lung injury,ALI)的作用,探讨肠道菌群调节LPS诱导的ALI的机制。方法 6周龄SD大鼠(体重170 g±10 g)随机分为对照组(CK组)和美罗培南组(... 详细信息

目的观察广谱抗生素诱导的肠道菌群改变对脂多糖(lipopolysaccharide,LPS)诱导的急性肺损伤(acute lung injury,ALI)的作用,探讨肠道菌群调节LPS诱导的ALI的机制。方法 6周龄SD大鼠(体重170 g±10 g)随机分为对照组(CK组)和美罗培南组(M组),每组10只。M组大鼠使用美罗培南实现肠道细菌清除。采集两组大鼠粪便标本,进行16S rDNA微生物群落分析和短链脂肪酸(short chain fatty acids,SCFAs)分析。LPS诱导大鼠ALI后抽血检测血SCFAs、血清炎症因子水平、肺组织病理切片、肺湿/干重比及大鼠肺微血管内皮细胞单层通透性等。结果美罗培南给药后肠道菌群多样性和构成发生了变化;M组大鼠粪便中丙酸、丁酸、异丁酸、戊酸、异戊酸、己酸水平均较CK组下降,以戊酸下降最为显著(t=9.242,P<0.001);M组大鼠血浆中戊酸和己酸水平较CK组显著下降,以血液中的戊酸水平降低最显著(t=4.000,P<0.005);LPS诱导的ALI后M组大鼠血清炎症因子水平较CK组无显著升高;两组间病理切片提示炎症细胞浸润无显著差异;M组大鼠肺湿/干重比较CK组显著升高(P<0.050);戊酸作用后显著改善LPS诱导的大鼠肺微血管内皮细胞单层通透性增高(t=8.134,P<0.001)。结论美罗培南应用可以造成大鼠肠道菌群失调与SCFAs水平的变化。肠道菌群可以通过血液中戊酸水平的改变实现对ALI的保护作用。

关键词：肠道菌群戊酸急性肺损伤肺微血管内皮细胞

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右美托咪啶联合用药新方案用于重症医学科长时间镇静的研究

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中国现代医药杂志 2012年第12期14卷 20-22页

作者：徐进步汪允珍施青山江苏省连云港市第二人民医院重症医学科 222000

目的比较在重症医学科内长时间镇静状态下不同镇静方案的差别,找出较适合长时间镇静的方案。方法选取在我院重症医学科住院的患者80例,随机分为4组,即右美托咪啶持续泵注联合咪达唑仑间断应用组(A组,n=20),右美托咪啶与咪达唑仑联合持... 详细信息

目的比较在重症医学科内长时间镇静状态下不同镇静方案的差别,找出较适合长时间镇静的方案。方法选取在我院重症医学科住院的患者80例,随机分为4组,即右美托咪啶持续泵注联合咪达唑仑间断应用组(A组,n=20),右美托咪啶与咪达唑仑联合持续泵注组(B组,n=20),咪达唑仑单独持续泵入组(C组,n=20),右美托咪啶单独持续泵入组(D组,n=20)。镇静目标为肌肉活动评分法(monitor activity assessments cale,MAAS)3分,镇静过程中持续心电监护,监测镇静满意程度,记录4组患者3日用药量变化,并监测患者不良反应发生率。比较4组患者后3种指标的差别。结果 A、B两组较C、D两组镇静满意程度显著增高,用药量变化幅度较小,不良反应较少,其中又以A组不良反应为最少,且与B组相比咪达唑仑用药量显著减少。结论右美托咪啶持续泵注联合咪达唑仑间断应用可显著减少长时间镇静所需药物用量,且减少不良反应。

关键词：右美托咪啶咪达唑仑长时间镇静镇静满意程度药物耐受性

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短暂脑缺血诱导大鼠海马PKC-ε膜转位及HSP70表达

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徐州医学院学报 2008年第5期28卷 285-289页

作者：徐进步许鹏程丰浩荣吴永平徐州医学院江苏省麻醉学重点实验室江苏徐州221002 徐州医学院病理学教研室

目的探讨蛋白激酶C-ε(protein kinase C,PKC-ε)和热休克蛋白70(heat shock protein,HSP70)在脑缺血耐受形成过程中的作用。方法将80只大鼠分为短暂脑缺血组和假手术组(n=40),每大组根据取材时间又分为缺血后1 h、6 h、1 d、2 d、4 d ... 详细信息

目的探讨蛋白激酶C-ε(protein kinase C,PKC-ε)和热休克蛋白70(heat shock protein,HSP70)在脑缺血耐受形成过程中的作用。方法将80只大鼠分为短暂脑缺血组和假手术组(n=40),每大组根据取材时间又分为缺血后1 h、6 h、1 d、2 d、4 d 5个亚组(n=8)。应用大脑中动脉阻闭(middle cerebral artery occlusion,MCAO)方法和免疫印迹(western blot)技术观察大鼠短暂脑缺血20 min后海马神经细胞不同时间点PKC-ε膜转位及HSP70表达的变化。结果假手术组各时间点的PKC-ε与HSP70无明显变化。在短暂脑缺血组,缺血后1 h海马神经细胞胞质PKC-ε的含量开始下降,至1天达最低点,持续到4天;而胞膜PKC-ε的变化趋势相反,含量于6 h显著增高,1天达到高峰,持续到4天;短暂缺血1 h后HSP70的表达无明显增加,6 h增多明显,1天达最高,持续至4天均处于较高水平。结论短暂脑缺血诱导海马神经细胞PKC-ε膜转位和HSP70表达增加,可能是短暂脑缺血诱导缺血耐受的机制。

关键词：脑缺血蛋白激酶C-ε 热休克蛋白70 缺血耐受

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呼吸机波形监测对AECOPD患者无创通气的意义

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徐州医学院学报 2013年第4期33卷 246-249页

作者：徐进步汪允珍施青山曹莉锋连云港市第二人民医院重症医学科江苏连云港222000

目的探讨通过呼吸机波形监测改善人机协调性对改善慢性阻塞性肺疾病急性加重（acute exarceba—tion of chronic obstructed pulmonary disease，AECOPD）患者无创通气疗效的作用。方法65例需无创通气的AE—COPD患者，随机分为处理组和... 详细信息

目的探讨通过呼吸机波形监测改善人机协调性对改善慢性阻塞性肺疾病急性加重（acute exarceba—tion of chronic obstructed pulmonary disease，AECOPD）患者无创通气疗效的作用。方法65例需无创通气的AE—COPD患者，随机分为处理组和对照组。2组患者均给予无创通气及常规药物治疗。对照组在无创通气时仅按规范设置呼吸机参数；处理组在此基础上通过监测呼吸机波形调节呼吸机设置，改善人机协调性。观察2组患者在血气分析、呼吸频率、呼吸机耐受程度、无创通气时间及插管率方面的差异。结果处理组患者二氧化碳分压（PaCO2）与pH改善速度显著快于对照组，治疗后2～6h呼吸机耐受程度好于对照组，无创通气时间显著缩短（P〈0．05）；2组患者呼吸频率与氧合指数（PaO2／FiO2）改善程度无统计学差异，插管率无统计学差异（P〉0．05）。结论通过监测呼吸机波形来改善人机协调性，可以使无创通气AECOPD患者尽早改善阻塞性通气状态，更好地耐受无创通气，并缩短无刨通气时间。

关键词：慢性阻塞性肺疾病急性加重无创通气人机协调性呼吸机波形

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An Improved Method for Evaluating Hydrocarbon Generation of Shale:A Case Study of the Lower Cretaceous Qingshankou Formation Shale in the Songliao Basin

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Acta Geologica Sinica(English Edition) 2023年第6期97卷 1825-1835页

作者： ZHANG Yuchen WANG Min LI jinbu ZHAO Chen YAN Yu xu Liang DENG Zixiao National Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)QingdaoShandong 266580China School of Geosciences China University of Petroleum(East China)QingdaoShandong 266580China Exploration and Development Research Institute of PetroChina Daqing Oilfield Company DaqingHeilongjiang 163712China

Because of the influence of hydrocarbons,especially adsorbed hydrocarbons,on the detection of cracked hydrocarbon(S2)and total organic carbon(TOC),the hydrogen index(HI)-based hydrocarbon generation model deviates from actual *** this study,the shale in the first member of the Qingshankou Formation in the central depression of the Songliao Basin,where in northeastern China,was taken as the research object and a correction method for S2 and TOC was *** correcting the experimental results of different maturity samples,the actual hydrocarbon generation model has been revealed,the differences before and after correction compared,and the evolutionary characteristics of the adsorbed hydrocarbon content were *** results show that the organic matter enters the hydrocarbon generation threshold at R_(o)-0.5% and reaches the hydrocarbon generation peak at R_(o)-1.0% and that the hydrocarbon generation process ends at R_(o)-1.3%.The hydrocarbon generation model established based on the measured values has a‘lag effect’compared to actual values,and this extends the hydrocarbon generation window of organic matter and delays the hydrocarbon generation *** the increase of maturity,adsorbed hydrocarbon content shows the characteristics of‘first increasing,then stabilizing,and then decreasing’,and reache s the most abundant stage at Roof 0.9%-1.3%.

关键词： hydrocarbon generation hydrogen index correction adsorbed hydrocarbon shale oil Qingshankou Formation Heilongjiang Province

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Plant Intron-Splicing Efficiency Database(PISE):exploring splicing of~1,650,000 introns in Arabidopsis,maize,rice,and soybean from~57,000 public RNA-seq libraries

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Science China(Life Sciences) 2023年第3期66卷 602-611页

作者： Hong Zhang jinbu Jia Jixian Zhai Department of Biology School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Institute of Plant and Food Science Southern University of Science and TechnologyShenzhen 518055China Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Institute of Plant and Food ScienceDepartment of BiologySouthern University of Science and TechnologyShenzhen 518055China

Intron retention is the most common alternative splicing event in plants and plays a crucial role in the responses of plants to environmental *** a large number of RNA-seq libraries from different treatments and genetic mutants stored in public domains,a resource for querying the intron-splicing ratio of individual intron is still ***,we established the first-ever large-scale splicing efficiency database in any *** database includes over 57,000 plant public RNA-seq libraries,comprising 25,283 from Arabidopsis,17,789 from maize,10,710 from rice,and 3,974 from soybean,and covers a total of 1.6 million introns in these four *** addition,we manually curated and annotated all the mutant-and treatment-related libraries as well as their matched controls included in our library collection,and added graphics to display intron-splicing efficiency across various tissues,developmental stages,and stress-related *** result is a large collection of 3,313treatment conditions and 3,594 genetic mutants for discovering differentially regulated splicing *** online database can be accessed at https://***/.

关键词： splicing RNA-seq plant database

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Accounting information quality,governance efficiency and capital investment choice

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China Journal of Accounting Research 2016年第4期9卷 251-266页

作者： jinbu Zhai Yutao Wang School of Public Finance Central University of Finance and Economics School of Accounting Central University of Finance and Economics

This paper examines the relationship between accounting information quality and capital investment choice from the perspective of accounting information's governance *** capital investment choice as the correlation of growth of operating income between company and industry,this paper investigates whether and to what extent companies focus on their core *** results show that the higher the quality of publicly listed firms' accounting information,the stronger that correlation,particularly when the corporate governance of the listed company is *** findings imply that accounting information quality can thus optimize the capital investment choice,which complements and strengthens the functioning of corporate ***,regulators should pay more attention to the market's power to supervise the behavior of listed firms,improve the governance functions of accounting information and increase the efficiency of capital allocation.

关键词： Accounting information quality Governance efficiency Capital investment choice

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A Volumetric Model for Evaluating Tight Sandstone Gas Reserves in the Permian Sulige Gas Field,Ordos Basin,Central China

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Acta Geologica Sinica(English Edition) 2019年第2期93卷 386-399页

作者： CUI Mingming FAN Aiping WANG Zongxiu GAO Wanli LI jinbu LI Yijun Key Laboratory of Shale oil and Gas Geological Survey Institute of Geomechanics Chinese Academy of Geological Sciences College of Geological Science & Engineering Shandong University of Science and Technology Institute of Exploration and Development Changqing Oilfield Company Petrochina

To accurately measure and evaluate reserves is critical for ensuring successful production of unconventional oil and gas. This work proposes a volumetric model to evaluate the tight sandstone gas reserves of the Permian Sulige gas field in the Ordos Basin. The reserves can be determined by four major parameters of reservoir cutoffs, net pay, gas-bearing area and compression factor Z, which are controlled by reservoir characteristics and sedimentation. Well logging, seismic analysis, core analysis and gas testing, as well as thin section identification and SEM analysis were used to analyze the pore evolution and pore-throat structure. The porosity and permeability cutoffs are determined by distribution function curve,empirical statistics and intersection plot. Net pay and gas-bearing area are determined based on the cutoffs, gas testing and sand body distribution, and the compression factor Z is obtained by gas component. The results demonstrate that the reservoir in the Sulige gas field is characterized by ultralow porosity and permeability, and the cutoffs of porosity and permeability are 5% and 0.15×10^(–3) μm^2, respectively. The net pay and gas-bearing area are mainly affected by the sedimentary facies, sand body types and distribution. The gas component is dominated by methane which accounts for more than 90%, and the compression factor Z of H_8(P_2h_8) and S_1(P_1s_1) are 0.98 and 0.985, respectively. The distributary channels stacked and overlapped, forming a wide and thick sand body with good developed intergranular pores and intercrystalline pores. The upper part of channel sand with good porosity and permeability can be sweet spot for gas exploration. The complete set of calculation systems proposed for tight gas reserve calculation has proved to be effective based on application and feedback. This model provides a new concept and consideration for reserve prediction and calculation in other areas.

关键词： tight sandstone reservoir volumetric gas reserve Permian Sulige gas field Ordos Basin

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Financial Shocks,Deleveraging and Macroeconomic Fluctuations in China

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China Finance and Economic Review 2022年第3期11卷 23-45页

作者： Ziguan Zhuang jinbu Zou Dingming Liu School of Finance of Zhongnan University of Economics and Law Economics and Management School of Wuhan University Wang Yanan Institute for Studies in Economics Xiamen University

To deleverage is one of the major tasks for the supply-side structural reform in China,and to steadily deleverage in order is the key to fending offand defusing financial *** paper uses the economic statistics of China around 2016 to depict the“expansion-contraction”fluctuations with Chinese macroeconomy during the *** this realistic context,it constructs a financial business cycle model based on the financial accelerator theory and attempts to use default cost changes to introduce financial shocks and understand China’s macroeconomic fluctuations in the deleveraging context in the perspective of unanticipated and anticipated *** of the numerical model simulation show that before and after the deleveraging,the fluctuations of credit,leverage ratio,credit spread and other major macroeconomic variables originate not only from the changes with unanticipated default *** changes with default cost can similarly explain the“expansion-contraction”macroeconomic fluctuations in recent years and offer a new perspective into the fluctuations during ***,government,when practicing deleveraging policies,is advised to take into full consideration not only the actual changes with default cost,but also anticipated factors of financial institutions.

关键词： default costs deleveraging financial shocks anticipated shocks

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