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Enhancer of zeste homolog 2 contributes to apoptosis by inactivating janus kinase 2/signal transducer and activator of transcription signaling in inflammatory bowel disease

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World Journal of Gastroenterology 2021年第22期27卷 3073-3084页

作者： Jie Zhou Yang Yang yi-Ling Wang Yue Zhao Wen-jing Ye Si-Yao Deng jin-yi lang Shun Lu Department of Radiation Oncology Sichuan Cancer HospitalChengdu 610041Sichuan ProvinceChina Department of Oncology The Third People's Hospital of ChengduChengdu 255415Sichuan ProvinceChina Department of School of Medicine University of Electronic Science and Technology of ChinaChengdu 397992Sichuan ProvinceChina Department of Radiological Protection Radiation Oncology Key Laboratory of Sichuan ProvinceChengdu 229717Sichuan ProvinceChina

BACKGROUND Inflammatory bowel disease(IBD)is a prevalent worldwide health problem featured by relapsing,chronic gastrointestinal *** of zeste homolog 2(EZH2)is a critical epigenetic regulator in different pathological models,such as cancer and ***,the role of EZH2 in the IBD development is still *** To explore the effect of EZH2 on IBD progression and the underlying *** The IBD mouse model was conducted by adding dextran sodium sulfate(DSS),and the effect of EZH2 on DSS-induced colitis was assessed in the *** function of EZH2 in regulating apoptosis and permeability was evaluated by Annexin V-FITC Apoptosis Detection Kit,transepithelial electrical resistance analysis,and Western blot analysis of related markers,including Zona occludens 1,claudin-5,and occludin,in NCM460 and fetal human colon(FHC)*** mechanical investigation was performed by quantitative reverse transcriptionpolymerase chain reaction,Western blot analysis,and chromatin immunoprecipitation *** The colon length was inhibited in the DSS-treated mice and was enhanced by the EZH2 depletion in the *** treatment caused a decreased histological score in the mice,which was reversed by EZH2 *** inflammatory cytokines,such as tumor necrosis factor-α,interleukin-6,and interleukin-1β,were induced in the DSS-treated mice,in which the depletion of EZH2 could reverse this ***,the tumor necrosis factor-αtreatment induced the apoptosis of NCM460 and FHC cells,in which EZH2 depletion could reverse this effect in the ***,the depletion of EZH2 attenuated permeability of colonic epithelial ***,the depletion of EZH2 or EZH2 inhibitor GSK343 was able to enhance the expression and the phosphorylation of janus kinase 2(JK2)and signal transducer and activator of transcription in the NCM460 and FHC ***,EZH2 inactivated JAK2 expression by regulating histone ***2 inhibitor

关键词： Inflammatory bowel disease Apoptosis Enhancer of zeste homolog 2 JAK2 Permeability Inflammatory bowel disease therapy

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New Evidence and Perspectives on the Management of Hepatocellular Carcinoma with Portal Vein Tumor Thrombus

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Journal of Clinical and Translational Hepatology 2017年第2期5卷 169-176页

作者： Jun yin Wen-Tao Bo Jian Sun Xiao Xiang jin-yi lang Jian-Hong zhong Le-Qun Li Department of Radiation Oncology Sichuan Cancer Hospital & Institute Sichuan Cancer Center School of Medicine University of Electronic Science and Technology of China Chengdu China Department of Hepatobiliary Surgery Sichuan Cancer Hospital &Institute Sichuan Cancer Center School of Medicine University of Electronic Science and Technology of China Chengdu China Department of Medical Affairs ZiBo Hospital of Integrated Traditional Chinese and Western Medicine Zibo China Department of Hepatobiliary Surgery Affiliated Tumor Hospital of Guangxi Medical University Nanning China

Portal vein tumor thrombosis (PVTT) is an intractable condition but common phenomenon in hepatocellular carcinoma (HCC).HCC patients with PVTT may have worse liver function,a higher chance of comorbidity related to portal hypertension,lower tolerance to treatment and poorer *** Western guidelines,patients are offered palliative treatment with sorafenib or other systemic agents because HCC with PVTT is grouped together with metastatic HCC during the planning of its *** recent years,various treatment options have become available for patients with HCC and *** has also shifted toward evidencebased ***,policies for the management of HCC with PVTT have not been *** comprehensive literature review aims to present current and available management options for patients with HCC and *** is mainly based on studies published after 2010.

关键词： Hepatic resection Hepatocellular carcinoma Portal vein tumor thrombosis Transarterial chemoembolization

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CACA guidelines for holistic integrative management of nasopharyngeal carcinoma

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Holistic Integrative Oncology 2023年第1期2卷 213-247页

作者： Ling-Long Tang Lin Chen Chao-Su Hu Jun-Lin yi jin-Gao Li Xia He Feng jin Xiao-Dong Zhu Xiao-Zhong Chen ying Sun Liang-Fang Shen Kun-Yu Yang Mei Feng Man Hu Yun-Fei Xia Ren-Sheng Wang Chuan-Ben Chen Tai-Xiang Lu Shao-Jun Lin jin-yi lang Jun Ma Department of Radiation Oncology State Key Laboratory of Oncology in South ChinaGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-Sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Radiation Oncology Fudan University Shanghai Cancer CenterShanghai 200032P.R.China Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021P.R.China Department of Radiotherapy Jiangxi Cancer HospitalNanchangJiangxi 330029P.R.China Department of Clinical Laboratory Affiliated Cancer Hospital of Nanjing Medical UniversityJiangsu Cancer HospitalJiangsu Institute of Cancer ResearchJiangsuZhejiang 210000P.R.China Guizhou Cancer Hospital of Guizhou Medical University GuiyangGuizhou 550000P.R.China Department of Oncology Affiliated Wuming Hospital of Guangxi Medical UniversityNanningGuangxi 530000P.R.China Cancer Hospital of the University of Chinese Academy of Sciences Zhejiang Cancer HospitalInstitute of Cancer and Basic Medicine(IBMC)Chinese Academy of SciencesHangzhouZhejiang 310000P.R.China Department of Radiation Oncology Xiangya Hospital of Central South UniversityChangshaHunan 410008P.R.China Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022P.R.China Department of Radiation Oncology Sichuan Cancer Hospital and InstituteSichuan Cancer CenterSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan 610041P.R.China Department of Radiation Oncology Shandong Cancer Hospital and InstituteShandong First Medical University and Shandong Academy of Medical SciencesJinanShandong 250117P.R.China Department of Radiation Oncology The First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530000P.R.China Department of Radiation Oncology Fujian Cancer HospitalFujian Medical University Department of

Purpose Nasopharyngeal carcinoma(NPC)is a malignant epithelial tumor originating in the nasopharynx and is particularly prevalent in southern ***,international guidelines,such as NCCN or ESMO,fail to adequately coincide with clinical practice in China,making it difficult to achieve precision personalized therapy in *** aim of this guideline is to better promote a"Multidisciplinary Team to Holistic Integrative Medicine"(MDT to HIM)system for the prevention,screening,diagnosis,treatment,and rehabilitation of *** The China Anti-Cancer Association(CACA)invited domestic multi-disciplinary experts,involving radiologists,oncologists,surgeons,pathologists,herbalists,physiatrists,and psychologists,to write,discuss,and revise the *** on the integration of research evidence,clinical experience,and patient needs,the domestic experts have iteratively developed these guidelines to provide proper and feasible management of *** and conclusion The CACA Guidelines for Holistic Integrative Management of Nasopharyngeal Carcinoma are more suitable for China’s clinical practice,highlight Chinese characteristics,and have important clinical significance.

关键词： Nasopharyngeal carcinoma China anti-Cancer Association(CACA) Integrated guideline

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Single-cell transcriptomic profiling reveals ZEB1-mediated regulation in microglial subtypes and the impact of exercise on neuroinflammatory responses

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Traditional Medicine Research 2025年第2期10卷 55-64页

作者： jin-Fang Wu yi-Sheng Chen Yu-Chun Xie Department of Rehabilitation Medicine Jiangsu Province Geriatric HospitalNanjing 210024China Fujian Key Laboratory of Toxicant and Drug Toxicology Medical CollegeNingde Normal UniversityNingde 352100China Central Laboratory Jiangsu Province Geriatric HospitalNanjing 210024China

Background:This study aims to identify distinct cellular subtypes within brain tissue using single-cell transcriptomic analysis,focusing on specific biomarkers that differentiate cell types and the effects of traditional and exercise ***:Four samples were analyzed:older control(OC),older exercise(OE),younger control(YC),and younger exercise(YE).Single-cell RNA sequencing was used to distinguish cellular subtypes through their biomarker *** visualization included violin and t-SNE plots to illustrate biomarker expression across cell clusters such as oligodendrocytes,microglia,and ***,BV2 cells were exposed to amyloid-beta fragments to simulate Alzheimer’s disease,assessing the impact of exercise-induced cellular ***:Distinct cellular subtypes were identified:oligodendrocytes(MBP,St18),microglia(Dock8),and astrocytes(Aqp4,Gpc5).Sample OE was predominantly oligodendrocytes,while YE had more astrocytes,inhibitory neurons,and Canal-Retzius *** showed a significant presence of Olfm3+ganglion ***1 gene knockout revealed changes in SMAD family gene expression,which regulate *** stress levels were also ***:This profiling enhances our understanding of brain cellular functions and interactions,potentially informing targeted therapies in neurological *** may influence brain cell immune responses and cell death pathways by regulating specific gene expressions,offering new insights for treating neuroinflammation and degeneration.

关键词： single-cell transcriptomics brain tissue cellular subtypes biomarker expression neuronal diversity

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Kangfuxin solution alleviates esophageal stenosis after endoscopic submucosal dissection:A natural ingredient strategy

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World Journal of Gastroenterology 2025年第1期31卷 110-125页

作者： Xin Zhou Dan Ma yi-Xiang He jing jin Hong-Lian Wang Yun-Feng Wang Fan Yang Jian-Qin Liu Jie Chen Zhi Li Department of Spleen and Stomach Diseases the Affiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina The Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Digestive System Diseases of Luzhou City The Affiliated Traditional Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Gastroenterology Changhai HospitalNaval Medical UniversityShanghai 20082China Research Center of Integrated Chinese and Western Medicine The Affiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina School of Integrated Traditional Chinese and Western Clinical Medicine North Sichuan Medical CollegeNanchong637100Sichuan ProvinceChina

BACKGROUND Esophageal stricture ranks among the most significant complications following endoscopic submucosal dissection(ESD).Excessive fibrotic repair is a typical pathological feature leading to stenosis after *** To examine the effectiveness and underlying mechanism of Kangfuxin solution(KFX)in mitigating excessive fibrotic repair of the esophagus *** Pigs received KFX at 0.74 mL/kg/d for 21 days after esophageal full circumferential *** examinations occurred on days 7 and 21 *** vitro,recombinant transforming growth factor(TGF)-β1(5 ng/mL)induced a fibrotic microenvironment in primary esophageal fibroblasts(pEsF).After 24 hours of KFX treatment(at 1.5%,1%,and 0.5%),expression ofα-smooth muscle actin-2(ACTA2),fibronectin(FN),and type collagen I was *** signaling was analyzed,including TGF-β1,Smad2/3,and phosphor-smad2/3(p-Smad2/3).RESULTS Compared to the Control group,the groups treated with KFX and prednisolone exhibited reduced esophageal stenosis,lower weight loss rates,and improved food tolerance 21 d after *** treatment,Masson staining revealed thinner and less dense collagen fibers in the submucosal ***,the expression of fibrotic effector molecules was notably ***,KFX downregulated the transduction levels of fibrotic functional molecules such as TGF-β1,Smad2/3,and p-Smad2/*** vitro,pEsF exposed to TGF-β1-induced fibrotic microenvironment displayed increased fibrotic activity,which was reversed by KFX treatment,leading to reduced activation of ACTA2,FN,and collagen *** 1.5%KFX treatment group showed decreased expression of p-Smad 2/3 in TGF-β1-activated *** KFX showed promise as a therapeutic option for post-full circumferential esophageal ESD strictures,potentially by suppressing fibroblast fibrotic activity through modulation of the TGF-β1/Smads signaling pathway.

关键词： Kangfuxin solution Natural component Endoscopic submucosal dissection Esophagus stricture Fibrosis

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替拉扎明调节乏氧诱导鼻咽癌细胞HIF-1α和OPN mRNA的表达及其对放射增敏的作用

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癌症 2010年第2期29卷 132-136页

作者：徐鹏黄建鸣任源查晓邓碧芳吴俊辉郎锦义广西医科大学广西南宁530021 四川省肿瘤医院四川成都610041

背景与目的:乏氧细胞毒性药物联合肿瘤放化疗是肿瘤治疗的一种重要途径,替拉扎明(tirapazamine,TPZ)是最受瞩目的乏氧细胞毒性药物之一,研究证实替拉扎明联合放疗作用于肿瘤细胞具有协同效应,但在乏氧条件下是否能下调乏氧诱导相关基因... 详细信息

背景与目的:乏氧细胞毒性药物联合肿瘤放化疗是肿瘤治疗的一种重要途径,替拉扎明(tirapazamine,TPZ)是最受瞩目的乏氧细胞毒性药物之一,研究证实替拉扎明联合放疗作用于肿瘤细胞具有协同效应,但在乏氧条件下是否能下调乏氧诱导相关基因未见报道,因此本实验研究人鼻咽癌细胞HNE-1EB+和CNE-1EB-乏氧相关基因乏氧诱导因子1α(HIF-1α)和骨桥蛋白(osteopontin,OPN)的表达差异以及TPZ的放射增敏作用。方法:在常氧和乏氧条件下,用MTT法测定TPZ对HNE-1和CNE-1细胞的生长抑制作用,计算出IC50;用RT-PCR测定TPZIC10作用24h后HNE-1和CNE-1细胞HIF-1α和OPN mRNAs的表达;以及用克隆形成法测定TPZ预作用6h和1～6Gy60Coγ射线照射后细胞的存活率,用单击多靶数学模型拟合剂量-生存曲线,计算出D0、Dq和放射增敏比(SER)。结果:TPZ对HNE-1和CNE-1细胞的IC50在常氧条件下分别为34.81μmol/L、35.02μmol/L,在乏氧条件下分别为30.20μmol/L和28.48μmol/L;TPZ能明显下调HNE-1细胞乏氧后HIF-1α和OPN的表达,对CNE-1HIF-1α和OPN的表达无明显作用;放射剂量-生存曲线符合单击多靶数学模型特征;HNE-1aero和HNE-1hypox的D0和Dq分别为0.89Gy、0.28Gy,1.47Gy和0.44Gy;HNE-1hypox/TPZ的D0和Dq分别为0.66Gy和0.21Gy;CNE-1aero和CNE-1hypox的D0和Dq分别为0.72Gy、0.68Gy,0.95Gy和0.56Gy;CNE-1hypox/TPZ的D0和Dq分别为0.85Gy和0.79Gy。结论:TPZ对HNE-1EB+细胞明显的放射增敏作用可能与其下调乏氧诱导的HIF-1α和OPN表达有关,以及具有乏氧修饰剂的作用。

关键词：替拉扎明鼻咽癌细胞放射增敏乏氧诱导因子1α 骨桥蛋白

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The Chinese Society of Clinical Oncology(CSCO)clinical guidelines for the diagnosis and treatment of nasopharyngeal carcinoma

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Cancer Communications 2021年第11期41卷 1195-1227页

作者： Ling-Long Tang Yu-Pei Chen Chuan-Ben Chen Ming-Yuan Chen Nian-Yong Chen Xiao-Zhong Chen Xiao-jing Du Wen-Feng Fang Mei Feng jin Gao Fei Han Xia He Chao-Su Hu De-sheng Hu Guang-Yuan Hu Hao Jiang Wei Jiang Feng jin jin-yi lang jin-Gao Li Shao-Jun Lin Xu Liu Qiu-Fang Liu Lin Ma Hai-Qiang Mai Ji-Yong Qin Liang-Fang Shen ying Sun Pei-Guo Wang Ren-Sheng Wang Ruo-Zheng Wang Xiao-Shen Wang ying Wang Hui Wu Yun-Fei Xia Shao-Wen Xiao Kun-Yu Yang Jun-Lin yi Xiao-Dong Zhu Jun Ma Department of Radiation Oncology State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center of Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhouGuangdong 510060P.R.China Department of Radiation Oncology Fujian Provincial Cancer HospitalFujian Medical University Department of Radiation OncologyTeaching Hospital of Fujian Medical University Provincial Clinical CollegeCancer Hospital of Fujian Medical UniversityFuzhouFujian 350014P.R.China Department of Nasopharyngeal Carcinoma State Key Laboratory of Oncology in South ChinaCollaborative Innovation Centre for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Radiation Oncology Cancer CenterWest China HospitalSichuan UniversityChengduSichuan 610041P.R.China Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)Institute of Cancer and Basic Medicine(IBMC)Chinese Academy of Sciences HangzhouZhejiang 310000P.R.China State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineMedical Oncology DepartmentSun Yat-Sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Radiation Oncology Sichuan Cancer Hospital and InstituteSichuan Cancer CenterSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuan 610041P.R.China Department of Radiation Oncology Anhui Provincial Hospital Affiliated to Anhui Medical UniversityHefeiAnhui 230001P.R.China Department of Clinical Laboratory Affiliated Cancer Hospital of Nanjing Medical UniversityJiangsu Cancer HospitalJiangsu Institute of Cancer ResearchNanjingJiangsu 210000P.R.China Department of Radiation Oncology Fudan University Shanghai Cancer CenterShanghai 200032P.R.China Department of Radiotherapy Hubei Cancer HospitalTong

Nasopharyngeal carcinoma(NPC)is a malignant epithelial tumor originating in the nasopharynx and has a high incidence in Southeast Asia and North *** develop these comprehensive guidelines for the diagnosis and management of NPC,the Chinese Society of Clinical Oncology(CSCO)arranged a multi-disciplinary team comprising of experts from all sub-specialties of NPC to write,discuss,and revise the *** on the findings of evidencebased medicine in China and abroad,domestic experts have iteratively developed these guidelines to provide proper management of ***,the guidelines describe the screening,clinical and pathological diagnosis,staging and risk assessment,therapies,and follow-up of NPC,which aim to improve the management of NPC.

关键词： Chinese Society of Clinical Oncology CSCO Nasopharyngeal carcinoma Diagnosis Staging Risk Radiotherapy Chemotherapy Surgery Immunotherapy

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World Journal of Clinical Cases 2025年第6期13卷 24-29页

作者： Wen-jin Yuan yi-Juan Zheng Bing-Rong Zhang yi-Jie Lin You Li Yan-Yan Qiu Xue-Ping Yu Department of Infection Disease Clinical Medical Research Center for Bacterial and Fungal Infectious Diseases of Fujian provinceFujian Medical University Affiliated First Quanzhou HospitalQuanzhou 362000Fujian ProvinceChina

BACKGROUND Hydroxyurea,an antimetabolite,is frequently prescribed for various hemato-logical disorders,and its common side effects include gastrointestinal problems,cutaneous or mucosal lesions and pyrexia/*** SUMMARY This study reports the case of a 67-year-old woman who developed recurrent abdominal pain after 10 years of continuous hydroxyurea therapy for primary *** revealed an ileocecal *** discontinuing hydroxyurea therapy for 6 months,follow-up colonoscopy showed a significant reduction in the *** We consider cecal ulcers as a rare complication of hydroxyurea therapy which typically resolves upon stopping the drug.

关键词： Hydroxyurea Ulcer Abdominal pain Thrombocythemia Colonoscopy Case report

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二元复合驱采出水达标处理工艺研究

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全面腐蚀控制 2024年第11期38卷 163-165页

作者：李明高军丁进善陆潇金毅浪黄炜伦中国石油华北油田公司第五采油厂河北辛集052360 河北华北石油路桥工程有限公司河北任丘062552 中国石油华北油田公司第二采油厂河北霸州065700 中国石油长庆油田分公司第十一采油厂陕西西安710016 中国石油天然气股份有限公司西南油气田公司川西北气矿四川江油621709

二元复合驱采出水中存在较多的驱油剂、聚合物和表面活性剂,其处理难度高于常规水驱或聚驱的采出水。为实现此类采出水的达标处理,分析了聚合物、表面活性剂浓度对水质特性的影响,采用曝气-生化-物理过滤流程对水样进行深度处理,并进行... 详细信息

二元复合驱采出水中存在较多的驱油剂、聚合物和表面活性剂,其处理难度高于常规水驱或聚驱的采出水。为实现此类采出水的达标处理,分析了聚合物、表面活性剂浓度对水质特性的影响,采用曝气-生化-物理过滤流程对水样进行深度处理,并进行了现场验证。结果表明,当聚合物浓度从0mg/L增加~1000mg/L时,水样浊度和Zeta电位绝对值逐渐增大;当曝气强度为12h,气水比为30:1,生化处理时间8d,并采用纤维球作为滤料时,其处理效果最佳,滤后采出水的油、悬浮物和粒径中值均满足规范要求,符合达标回注的要求。

关键词：二元复合驱采出水达标聚合物表面活性剂曝气生化处理

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Modeling and optimization for oil well production scheduling

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Chinese Journal of Chemical Engineering 2016年第10期24卷 1423-1430页

作者： jin lang Jiao Zhao The Institute of Industrial Engineering and Logistics Optimization Northeastern University State Key Laboratory of Synthetical Automation for Process Industries Northeastern University School of Automobile Chang'an University

In this paper,an oil well production scheduling problem for the light load oil well during petroleum field exploitation was *** oil well production scheduling was to determine the turn on/off status and oil flow rates of the wells in a given oil reservoir,subject to a number of constraints such as minimum up/down time limits and well *** problem was formulated as a mixed integer nonlinear programming model that minimized the total production operating cost and start-up *** to the NP-hardness of the problem,an improved particle swarm optimization(PSO) algorithm with a new velocity updating formula was developed to solve the problem *** experiments on randomly generated instances were carried out to evaluate the performance of the model and the algorithm's *** with the commercial solver CPLEX,the improved PSO can obtain high-quality schedules within a much shorter running time for all the instances.

关键词： Oil well production Scheduling Mixed integer nonlinear programming(MINLP)Improved partide swarm optimization

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