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Agricultural Sciences in China 2009年第7期8卷 872-879页

作者： LI Ming-qian LU Qi-neng WU Xiao-Feng MAN Na-na CHEN Xiao-xue jin wei LU Xing-meng Laboratory of lnvertebrate Pathology College of Animal Sciences Zhejiang University Hangzhou 310029 P.R.China

Full gene sequence of RNA-dependent RNA polymerase （RdRp） from Bombyx mori infectious flacherie virus isolated in Zhejiang Province, China （Zhejiang01/CHN/2002） was cloned. The sequence was 1 920 nucleotides in length coding 639 amino acid residues. Sequences comparison of RdRp showed Zhejiang01/CHN/2002 was 99.7% nucleotide sequence and 99.1% amino acids sequence homology with Japanese strain. The RdRp sequence was aligned with 8 representative picorna（-like） viruses and 8 highly conserved regions were detected. The result indicated their relevance function. Phylogenetic tree of 14 picorna（-like） viruses which RdRp presumed protein sequences revealed that the viruses from Iflavirus genus formed an independent clade. The RdRp was successfully expressed in BmN cells using Bac-to-Bac expression system.

关键词： infectious flacherie virus RNA-dependent RNA polymerase phylogenetic analysis BmN cells expression

Social avoidance behavior in male tree shrews and prosocial behavior in male mice toward unfamiliar conspecifics in the laboratory

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Zoological Research 2020年第3期41卷 258-272页

作者： Rong-Jun Ni Yang Tian Xin-Ye Dai Lian-Sheng Zhao jin-xue wei Jiang-Ning Zhou Xiao-Hong Ma Tao Li Psychiatric Laboratory and Mental Health Center West China Hospital of Sichuan UniversityChengduSichuan 610041China Huaxi Brain Research Center West China Hospital of Sichuan UniversityChengduSichuan 610041China Chinese Academy of Science Key Laboratory of Brain Function and Diseases School of Life SciencesUniversity of Science and Technology of ChinaHefeiAnhui 230027China

Adult male tree shrews vigorously defend against intruding male conspecifics. However, the characteristics of social behavior have not been entirely explored in these males. In this study, male wild-type tree shrews(Tupaia belangeri chinensis)and C57 BL/6 J mice were first allowed to familiarize themselves with an open-field apparatus. The tree shrews exhibited a short duration of movement(moving) in the novel environment, whereas the mice exhibited a long duration of movement. In the 30 min social preference-avoidance test, target animals significantly decreased the time spent by the experimental tree shrews in the social interaction(SI)zone, whereas experimental male mice exhibited the opposite. In addition, experimental tree shrews displayed a significantly longer latency to enter the SI zone in the second 15 min session(targetpresent) than in the first 15 min session(targetabsent), which was different from that found in *** behavioral patterns in response to a conspecific male were also observed in male tree shrews and mice in the first, second, and third 5 min periods. Thus, social behaviors in tree shrews and mice appeared to be time dependent. In summary,our study provides results of a modified social preference-avoidance test designed for the assessment of social behavior in tree shrews. Our findings demonstrate the existence of social avoidance behavior in male tree shrews and prosocial behavior in male mice toward unfamiliar conspecifics. The tree shrew may be a new animal model, which differs from mice, for the study of social avoidance and prosocial behaviors.

关键词： Social avoidance behavior Prosocial behavior Open-field test Social preference-avoidance test Social interaction

Semiunitary Transforrnation and Isospectral Hamiltonians in Arbitrary Dimensional Space

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Communications in Theoretical Physics 2001年第7期36卷 25-28页

作者： RUAN Dong WANG xue-jin HUANG wei-Cheng SUN Hong-Zhou Department of Physics Tsinghua University Beijing 100084 China The Key Laboratory of Quantum Information and Measurements of MOE Tsinghua University Beijing 100084 China Center of Theoretical Nuclear Physics National Laboratory of Heavy Ion Accelerator Lanzhou 730000 China Department of Electronic Engineering Tsinghua University Beijing 100084 China The Key Laboratory of Quantum Information and Measurements of MOE Tsinghua University Beijing 100084 China Institute of Applied Chemistry Xinjiang University Urumqi 830046 China Department of Physics Tsinghua University Beijing 100084 China Center of Theoretical Nuclear Physics National Laboratory of Heavy Ion Accelerator Lanzhou 730000 China

By means of the complex Clifford algebra, a new realization of multi-dimensional semiunitary transformation is put forward and then applied to studying the isospectrality of nonrelativistic Hamiltonians of multi-dimensional quantum mechanical systems, in which the generalized Pauli coupling interaction and spin-orbit coupling interaction appear naturally. Moreover, it is shown that the semiunitary operators, together with the Hamiltonian of quantum mechanical system, satisfy the polynomially-deformed angular momentum algebra.

关键词： semiunitary transformation, action-angle approach, isospectrality of Hamiltonians, deformed angular momentum algebra

Chronic lithium treatment ameliorates ketamine-induced mania-like behavior via the PI3K-AKT signaling pathway

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Zoological Research 2022年第6期43卷 989-1004页

作者： Rong-Jun Ni Tian-Hao Gao Yi-Yan Wang Yang Tian jin-xue wei Lian-Sheng Zhao Pei-Yan Ni Xiao-Hong Ma Tao Li Psychiatric Laboratory and Mental Health Center West China Hospital of Sichuan UniversityChengduSichuan 610041China Affiliated Mental Health Center&Hangzhou Seventh People’s Hospital Zhejiang University School of MedicineHangzhouZhejiang 310013China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Science and Brain-machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhouZhejiang 310014China Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence GuangzhouGuangdong 510799China

Ketamine, a rapid-acting antidepressant drug, has been used to treat major depressive disorder and bipolar disorder(BD). Recent studies have shown that ketamine may increase the potential risk of treatment-induced mania in patients. Ketamine has also been applied to establish animal models of mania. At present, however, the underlying mechanism is still unclear. In the current study, we found that chronic lithium exposure attenuated ketamine-induced mania-like behavior and c-Fos expression in the medial prefrontal cortex(mPFC) of adult male mice. Transcriptome sequencing was performed to determine the effect of lithium administration on the transcriptome of the PFC in ketamine-treated mice, showing inactivation of the phosphoinositide 3-kinase(PI3K)-protein kinase B(AKT) signaling pathway. Pharmacological inhibition of AKT signaling by MK2206(40 mg/kg), a selective AKT inhibitor, reversed ketamine-induced ***, selective knockdown of AKT via AAVAKT-sh RNA-EGFP in the mPFC also reversed ketamine-induced mania-like behavior. Importantly,pharmacological activation of AKT signaling by SC79(40 mg/kg), an AKT activator, contributed to mania in low-dose ketamine-treated mice. Inhibition of PI3K signaling by LY294002(25 mg/kg), a specific PI3K inhibitor, reversed the mania-like behavior in ketamine-treated mice. However, pharmacological inhibition of mammalian target of rapamycin(mTOR)signaling with rapamycin(10 mg/kg), a specific mTOR inhibitor, had no effect on ketamine-induced mania-like behavior. These results suggest that chronic lithium treatment ameliorates ketamine-induced mania-like behavior via the PI3K-AKT signaling pathway, which may be a novel target for the development of BD treatment.

关键词： Lithium Ketamine Medial prefrontal cortex Bipolar disorder Manic PI3K-AKT signaling pathway

Depletion of microglia with PLX3397 attenuates MK-801-induced hyperactivity associated with regulating inflammation-related genes in the brain

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Zoological Research 2023年第3期44卷 543-555页

作者： Rong-Jun Ni Yi-Yan Wang Tian-Hao Gao Qi-Run Wang jin-xue wei Lian-Sheng Zhao Yang-Rui Ma Xiao-Hong Ma Tao Li Mental Health Center and Psychiatric Laboratory West China HospitalSichuan UniversityChengduSichuan 610041China Sichuan Clinical Medical Research Center for Mental Disorders ChengduSichuan 610044China Golden Apple jincheng NO.1 Secondary School ChengduSichuan 610213China Affiliated Mental Health Center&Hangzhou Seventh People’s Hospital Zhejiang University School of MedicineHangzhouZhejiang 310013China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Science and Brain-machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhouZhejiang 310014China Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence GuangzhouGuangdong 510799China

Acute administration of MK-801(dizocilpine),an N-methyl-D-aspartate receptor(NMDAR)antagonist,can establish animal models of psychiatric ***,the roles of microglia and inflammation-related genes in these animal models of psychiatric disorders remain ***,we found rapid elimination of microglia in the prefrontal cortex(PFC)and hippocampus(HPC)of mice following administration of the dual colony-stimulating factor 1 receptor(CSF1R)/c-Kit kinase inhibitor PLX3397(pexidartinib)in drinking *** administration of MK-801 induced hyperactivity in the open-field test(OFT).Importantly,PLX3397-induced depletion of microglia prevented the hyperactivity and schizophrenia-like behaviors induced by ***,neither repopulation of microglia nor inhibition of microglial activation by minocycline affected MK-801-induced ***,microglial density in the PFC and HPC was significantly correlated with behavioral *** addition,common and distinct glutamate-,GABA-,and inflammation-related gene(116 genes)expression patterns were observed in the brains of PLX3397-and/or MK-801-treated ***,10 common inflammation-related genes(CD68,CD163,CD206,TMEM119,CSF3R,CX3CR1,TREM2,CD11b,CSF1R,and F4/80)with very strong correlations were identified in the brain using hierarchical clustering *** correlation analysis demonstrated that the behavioral changes in the OFT were most significantly associated with the expression of inflammation-related genes(NLRP3,CD163,CD206,F4/80,TMEM119,and TMEM176a),but not glutamate-or GABA-related genes in PLX3397-and MK-801-treated ***,our results suggest that microglial depletion via a CSF1R/c-Kit kinase inhibitor can ameliorate the hyperactivity induced by an NMDAR antagonist,which is associated with modulation of immune-related genes in the brain.

关键词： Microglia Psychiatric disorders Prefrontal cortex Hippocampus Immunity Colony-stimulating factor 1 receptor

三维MiLE算法模型模拟连铸结晶器内的板坯凝固过程

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航空材料学报 2010年第3期30卷 10-13页

作者：薛建国金学伟姚耕耘北京科技大学机械工程学院北京100083 清华大学自动化系北京100084

建立了热流力耦合的三维非稳态有限元模型,对结晶器内钢水凝固过程中的传热、流动和变形情况进行了模拟。以尺寸为1.2mm×0.2m的Q235B板坯为研究对象,利用Procast软件的二次开发,求解出了结晶器区域内的温度场、流场和应变分布。计算结... 详细信息

建立了热流力耦合的三维非稳态有限元模型,对结晶器内钢水凝固过程中的传热、流动和变形情况进行了模拟。以尺寸为1.2mm×0.2m的Q235B板坯为研究对象,利用Procast软件的二次开发,求解出了结晶器区域内的温度场、流场和应变分布。计算结果表明,铸坯收缩量在不同方向上存在不同的分布状态。考虑气隙产生对传热的影响时,结晶器出口铸坯表面平均温度由1120.5℃增加到1289.7℃,坯壳厚度由16.1mm减少到14.2mm。

关键词：板坯连铸结晶器数值模拟 MiLE算法

Elevation of tumor necrosis factor-α,interleukin-1β and interleukin-6 levels in aortic intima of Chinese Guizhou minipigs with streptozotocin-induced diabetes

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Chinese Medical Journal 2007年第6期120卷 479-484页

作者： LU Lin ZHANG Qi PU Li-jin XU xue-wei ZHANG Rui-yan ZHANG Jian-sheng HU Jian YANG Zheng-kun LU An-kang DING Feng-hua SHEN Jie CHEN Qiu-jin LOU Sheng FANG Dan-hong SHEN wei-feng Department of Cardiology Ruijin Hospital Institute of Cardiovascular Disease Medical School of Jiaotong University Shanghai 200025 China

Background Large animal models with toxin-mediated pancreatic damage have been used extensively in researches with respect to diabetes mellitus and cardiovascular diabetic complications. The present study aimed to establish Chinese Guizhou minipig models with streptozotocin （STZ）-induced diabetes and characterize the animal models by analyzing inflammatory cytokine levels in aortic wall, such as tumor necrosis factor （TNF）-α, interleukin-1β （IL-1β） and interleukin-6 （IL-6）. Methods Twenty-two male Chinese Guizhou minipigs （age, 4 to 6 months; weight, 20 kg to 30 kg） were divided into STZ-induced diabetic group （n=-12） and control group （n=-10）. STZ （125 mg/kg） was administrated to induce hyperglycemia and afterwards insulin was used to control fasting blood glucose levels below 10 mmol/L. Oral glucose tolerance test （OGTT） was performed before and one month after STZ administration and serum concentrations of alanine transaminase, asparegine transaminase, albumin, blood urea nitrogen, creatinine, lipids and white blood cell count were measured before and six months later. Animals in both groups were euthanized after six months and pancreas was examined immunohistochemically for islet 13 cells. Aortic intima of diabetic minipigs and controls was analyzed for TNF-α level in tissue conditioned medium by Western blot. TNF-α, IL-β and IL-6 mFINA levels in aortic intima were assayed by reverse transcription and polymerase chain reaction （FIT-PCR）. Results Significant elevation in serum glucose levels was observed one month and six months after STZ induction （P〈0.001） and markedly increased OGTT values were noted, compared with baseline data. The normal pancreas had many irregular sized islets and small clusters of islet β cells, while in pancreas of diabetic minipigs islet β ceils almost disappeared. No statistical difference was notified in serum concentrations of biochemical examinations before and six months after STZ induction. Westem blot demonstrated dramatically in

关键词： diabetes meUitus streptozotocin tumor necrosis factor-α interleukin-1β interleukin-6

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小分子化合物丙戊酸促进大鼠坐骨神经的再生

中国临床康复 2006年第43期10卷 117-119页

作者：张为众崔树森薛金伟李锐吉林大学中日联谊医院手外科吉林省长春市130033

目的:观察小分子化合物丙戊酸对周围神经再生的作用。方法:实验于2005-07/10在吉林大学中日联谊医院动物实验室完成。选用成年雄性Wistar大鼠15只,随机分为假手术组、模型组和丙戊酸组3组(n=5)。模型组和丙戊酸组大鼠切断右侧坐骨神经... 详细信息

目的:观察小分子化合物丙戊酸对周围神经再生的作用。方法:实验于2005-07/10在吉林大学中日联谊医院动物实验室完成。选用成年雄性Wistar大鼠15只,随机分为假手术组、模型组和丙戊酸组3组(n=5)。模型组和丙戊酸组大鼠切断右侧坐骨神经制备单纯坐骨神经轴突切断模型,然后即刻行神经吻合术,假手术组不切断坐骨神经。丙戊酸组大鼠在神经修复术后喂食含丙戊酸水(300mg/(kg·d))16周,使血浆浓度达到50mg/L。16周后,各组大鼠取坐骨神经及双侧胫骨前肌进行组织形态学检查,观察再生的有髓神经纤维和神经再支配的肌纤维数量及大小。结果:15只大鼠全部进入结果分析。①模型组和丙戊酸组再生的单个有髓神经纤维的大小明显小于假手术组(P<0.001);模型组和丙戊酸组中再生神经有髓纤维数量是假手术组的3倍(P<0.001);丙戊酸组大鼠中有髓神经纤维数量明显高于模型组(P<0.05)。②模型组和丙戊酸组大鼠神经再支配胫骨前肌中单个肌纤维大小明显小于假手术组(P<0.05),丙戊酸组再支配胫骨前肌肌纤维数量明显高于模型组(P<0.05)。结论:丙戊酸并不能影响神经再支配肌肉组织中肌纤维的大小,但可使神经再支配肌肉中肌纤维数量显著增加,进而增强大鼠坐骨神经再生能力,提示丙戊酸对人体周围神经损伤具有潜在的临床应用价值。

关键词：周围神经丙戊酸神经再生

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诊断小神经纤维损伤的新靶点一皮肤活检

中华神经医学杂志 2010年第4期9卷 422-424页

作者：凌丽薛金伟张敏河北大学附属医院神经内科保定071000

小神经纤维是一类直径小于7um的小直径神经纤维，它们是周围神经纤维中最细小的部分，由于轴突直径和传导速率都非常小，与大直径神经纤维相比，更容易受到不利因素的损害，因此，小神经纤维受损症状常常是很多周围神经病的一个常见和... 详细信息

小神经纤维是一类直径小于7um的小直径神经纤维，它们是周围神经纤维中最细小的部分，由于轴突直径和传导速率都非常小，与大直径神经纤维相比，更容易受到不利因素的损害，因此，小神经纤维受损症状常常是很多周围神经病的一个常见和早期的表现。但目前常规电生理等检查技术主要检测大直径有髓鞘神经纤维的功能，

关键词：小神经纤维皮肤活检靶点诊断

补阳还五汤药浴对大鼠周围神经损伤再生影响的实验研究

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时珍国医国药 2008年第5期19卷 1066-1067页

作者：石杜鹃凌丽薛金伟黑龙江中医药大学黑龙江哈尔滨150040 吉林大学白求恩医学部吉林长春130021

目的研究补阳还五汤药浴对周围神经损伤后修复的作用。方法采用雌性Wistar大鼠90只,A组为补阳还五汤药浴组,B组为补阳还五汤口服组,C组为单纯端端缝合组,每组30只。造模采用大鼠坐骨神经横断损伤模型,造模成功后7 d起,A组大鼠放入补阳... 详细信息

目的研究补阳还五汤药浴对周围神经损伤后修复的作用。方法采用雌性Wistar大鼠90只,A组为补阳还五汤药浴组,B组为补阳还五汤口服组,C组为单纯端端缝合组,每组30只。造模采用大鼠坐骨神经横断损伤模型,造模成功后7 d起,A组大鼠放入补阳还五汤温热药液中游泳,隔日1次,5 min/次;B组用补阳还五汤制剂灌胃,隔日1次,3 ml/次;C组大鼠进行单纯端端缝合。3组均于术后6周取材进行电生理学、组织形态学观察。结果补阳还五汤药浴可以明显减少神经损伤后运动神经元和感觉神经元的死亡,防止支配肌肉的萎缩变性,促进损伤后功能的恢复。结论补阳还五汤药浴可以在神经元、损伤局部、终末效应器多个平面、多个角度对神经修复产生积极作用。

关键词：周围神经损伤与再生补阳还五汤药浴