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Mesenchymal-epithelial Transition Factor Regulates Monocyte Function during Mycobacterial Infection via Indoleamine 2,3-dioxygenase

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Current Medical Science 2022年第2期42卷 407-416页

作者： Bing-fen YANG Fei ZHAI Hong-juan AN jing JIANG Zhi-hong CAO Yan-hua LIU jin-wen su Ruo WANG Xiao-xing CHENG Tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and TreatmentSenior Department of Tuberculosisthe Eighth Medical Center of Chinese PLA General HospitalBeijing100091China

Objective Mycobacterium tuberculosis(Mtb),the causative agent of tuberculosis(TB),causes an estimated 1.6 million human deaths annually,but the pathogenesis of TB remains *** plays a critical role in the onset and outcome of *** study aimed to uncover the roles of innate and adaptive immunity in *** The gene expression profiles generated by RNA sequencing from human peripheral blood mononuclear cells(PBMCs)stimulated with or without Mtb strain H37Rv antigens were analyzed.A total of 973 differentially expressed mRNAs were *** The differentially expressed genes were enriched in innate immunity signaling *** mesenchymal-epithelial transition factor(MET)gene was significantly upregulated in CD14^(+)monocytes.A MET inhibitor improved the uptake of the BCG strain by monocytes and macrophages as well as inhibited the expression of indoleamine 2,3-dioxygenase(IDO).The expression of IDO was increased in PBMCs stimulated with Mtb antigens,and the IDO inhibitor promoted the expression of CD40,CD83,and *** Our results might provide clues regarding the immunomodulatory mechanisms used by Mtb to evade the host defense system.

关键词： monocytes mycobacteria mesenchymal-epithelial transition factor indoleamine 2,3-dioxygenase

The effect pathway of retinoic acid through regulation of retinoic acid receptor α in gastric cancer cells

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World Journal of Gastroenterology 2001年第5期7卷 662-666页

作者： su Liu Qiao Wu Zheng-Ming Chen wen-jin su The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering,The School of Life Sciences,Xiamen University,Xiamen 361005,Fujian Province,China Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering The School of Life Sciences Xiamen University Xiamen 361005 Fujian Province China.

AIM To evaluate the role of RARα gone in mediating the growth inhibitory effect of all-trans retinoic acid(ATRA) on gastric cancer cells. METHODS The expression levels of retinoic acid receptors(PARs)in gastric cancer cells were detected by Northern *** transfection and chlorophenicol acetyl transferase(CAT)assay were used to show the transcriptional activity of β retinoic acid response element (βPARE)and AP-1 *** growth inhibition was determined by MTT assay and anchorage-independent growth assay,*** transfection was performed by the method of Lipofectamine,and the cells were screened by G418. REsuLTS ATRA could induce expression level of RARα in MGC80-3,BGC-823 and SGC-7901 cells obviously, resulting in growth inhibition of these cell *** sense RARα gone was transfected into MKN-45 cells that expressed rather low level of RARα and could not be induced by ATPA,the cell growth was inhibited by ATPA *** contrast,when antisense RARα gone was transfected into BGC-823 cells,a little inhibitory effect by ATPA was seen,compared with the parallel BGC-823 *** transient transfection assay,ATPA effectively induced transcriptional activity of βRARE in MGC80-3, BGC-823,SGC-7902 and MKN/RARα cell lines,but not in MKN-45 and BGC/aRARα cell *** results were observed in measuring anti-AP-1 activity by ATPA in these cancer cell lines. CONCLUSION ATRA inhibits the growth of gastric cancer cells by up-regulating the level of RARα; RARα is the major mediator of ATRA action in gastric cancer cells;and adequate level of RARα is required for ATRA effect on gastric cancer cells.

关键词： receptor retinoic acid/pharmacology stomach neoplasm/drug therapy stomach neoplasm/pathology

Effect of noninvasive, positive pressure ventilation on patients with severe, stable chronic obstructive pulmonary disease： a meta-analysis

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Chinese Medical Journal 2013年第1期126卷 140-146页

作者： SHI Jia-xin XU jin suN wen-kui su Xin ZHANG Yan SHI Yi Department of Respiratory and Critical Care Medicine JinlingHospital Nanjing University School of Medicine Nanjing 210002China

Background This meta-analysis evaluated the effect of noninvasive, positive pressure ventilation on severe, stable chronic obstructive pulmonary disease （COPD）. Methods PUBMED, CNKI, Wanfang, EMBASE and the Cochrane trials databases were searched. Randomized controlled trials of patients with severe, stable COPD and receiving noninvasive positive pressure ventilation, compared with sham ventilation or no ventilation, were reviewed. The mortality, physiological and health related parameters were pooled to yield odds ratio （OR）, weighted mean differences or standardized mean differences （SMD）, with 95% confidence interval （CI）. Results Eight parallel and three crossover randomized controlled trials met the inclusion criteria. Pooled analysis for parallel, randomized controlled trials showed noninvasive positive pressure ventilation：（1） Did not affect the 12- or 24-month mortality （OR 0.82, 95% Ch 0.48 to 1.41）; （2） Improved the arterial carbon dioxide tension （SMD -0.88, 95% Ch -1.43 to -0.34）; （3） Did not improve forced expiratory volume in one second （SMD 0.20, 95% CI： -0.06 to 0.46）, maximal inspiratory pressure （SMD 0.01, 95% Ch -0.28 to 0.29） or 6-minute walk distance （SMD 0.17, 95% Ch -0.16 to 0.50）; （4） subgroup analysis showed noninvasive positive pressure ventilation improved the arterial carbon dioxide tension in hypercapnic patients. Pooled analysis for crossover randomized controlled trials did not show improvement in arterial blood gas or forced expiratory volume in one second with noninvasive positive pressure ventilation. Conclusions Noninvasive positive pressure ventilation improves the arterial carbon dioxide tension but does not improve the mortality, pulmonary function, or exercise tolerance and should be cautiously used in severe stable chronic obstructive pulmonary disease.

关键词： chronic obstructive pulmonary disease meta-analysis noninvasive positive pressure ventilation stable

Degradation of retinoid X receptor α by TPA through proteasome pathway in gastric cancer cells

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World Journal of Gastroenterology 2003年第9期9卷 1915-1919页

作者： Xiao-Feng Ye su Liu Qiao Wu Xiao-Feng Lin Bing Zhang Jia-Fa Wu Ming-Qing Zhang wen-jin su Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,School of Life Sciences,Xiamen University,Xiamen 361005,Fujian Province,China Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen 361005 Fujian Province China.

AIM: To investigate and determine the mechanism and signal pathway of tetradecanoylphorbol-1, 3-acetate (TPA) in degradation of RXRα.METHODS: Gastric cancer cell line, BGC-823 was used in the experiments. The expression level of R XRα protein was detected by Western blot. Nuclear and cytoplasmic protein fractions were prepared through lysis of cell and *** and translocation of RXRα were observed under laser-scanning confocal microscope through labeling specific anti-RXRα antibody and corresponding immunofiuorescent antibody as secondary antibody. Different inhibitors were used as ***: In BGC-823 cells, RXRα was expressed in the nucleus. When cells were treated with TPA, expression of RXRα was repressed in a time-dependent and TPAconcentration-dependent manner. Meanwhile, translocation of RXR from the nucleus to the cytoplasm occurred, also in a time-dependent manner. When cells were pre-incubated with proteasome inhibitor MG132 for 3 hrs, followed by TPA for another 12 hrs, TPA-induced RXRα degradation was inhibited. Further observation of RXRα translocation in the presence of MG132 showed that MG-132 could block TPAinduced RXRα redistribution. Conversely, when RXRαtranslocation was inhibited by LMB, an inhibitor for blocking protein export from the nucleus, TPA could not repress expression of RXRα.CONCLUSION: TPA could induce the degradation of RXRα protein in BGC-823 cells, and this degradation is time-and TPA-concentration-dependent. Furthermore, the degradation of RXRα by TPA is via a proteasome pathway and associated with RXRα translocation from the nucleus to the cytoplasm.

关键词：胃癌类维生素A受体X TPA BGC-823细胞

High precision measurement of formaldehyde in air with differential optical absorption spectroscopy

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Optoelectronics Letters 2007年第6期3卷 451-454页

作者： PENG Fu-min, XIE Pin-hua, LIN Yi-hui, LI Yu-jin, LI su-wen, QIN Min, and LIU wen-qing Key Laboratory of Environmental Optics and Technology, Chinese Academy of Sciences Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China Key Laboratory of Environmental Optics and Technology Chinese Academy of Sciences Hefei China

The formaldehyde (HCHO) measurement is described by differential optical absorption spectroscopy (DOAS) technique and the data processing method is discussed in detail, including the selection of retrieval wavelength band, the removing of interfering structures, the steps of data processing and the spectrum dealing with nonlinear method. The HCHO concentra- tion retrieved in different wavelength bands were compared, including relative error, repeatability and detection limit. As a result, the band from 314 to 332 nm was most suitable for the retrieval of HCHO. With these methods, high measurement precision of 1.9% was obtained and the detection limit of HCHO is less than 1.5 ppb. In addition, high linear relativity 0.9999 is achieved by measuring five different concentrations of HCHO with this system.

关键词：光检测技术精密度甲醛微分光学

Degradation of myofibrillar proteins by a myofibril-bound serine proteinase in the skeletal muscle of crucian carp

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Degradation of myofibrillar proteins by a myofibril-bound se...

基于Logistic回归建立霜自动判识模型

中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： Min-Jie Cao~(a,*) Xin-jin Jiang~(a,b) Hui-Chang Zhong~a Zhi-Jun Zhang~c wen-jin su~a

Myofibril-bound serine proteinase(MBSP)in the skeletal muscle of crucian carp was *** of myofibrillar proteins by the endogenous MBSP was *** heavy chain(MHC)degraded markedly when crucian carp myofibril was incubated at around 55℃ as shown by *** incubation of myofibril at 55℃ also caused the obvious degradation of tropomyosin,while the decomposition of other myofibrillar proteins such as α-actinin and actin was slight as detected by Western *** results suggest the existence of an endogenous myofibril associated proteinase in crucian carp myofibril,which efficiently cleaves MHC and *** proteinase inhibitors(Lima bean trypsin inhibitor,PMSF and benzamidine)greatly suppressed the degradation of MHC caused by the enzyme,while inhibitors for cysteine,metallo,and asparatic proteinases only partially or completely did not show any inhibitory effect,indicating that the endogenous proteinase is a serine *** specificity analysis using partial purified crucian carp MBSP suggested that the enzyme is a trypsin-like serine proteinase.

关键词： crucian carp degradation myofibril inhibition serine proteinase

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中国农业气象 2019年第8期40卷 534-542页

作者：朱华亮温华洋华连生金素文陈菁菁安徽省气象信息中心

利用安徽省23个典型气象站2003-2017年观测数据,根据无霜日的气象要素阈值条件进行质量控制,在此基础上,构建各气象站基于Logistic回归的霜自动判识模型,并对模型的霜判识效果进行评估。结果表明:(1)通过气温、风速和降水量等气象要素阈... 详细信息

利用安徽省23个典型气象站2003-2017年观测数据,根据无霜日的气象要素阈值条件进行质量控制,在此基础上,构建各气象站基于Logistic回归的霜自动判识模型,并对模型的霜判识效果进行评估。结果表明:(1)通过气温、风速和降水量等气象要素阈值,能够有效判定出安徽各站当日无霜现象;(2)各气象站的霜判识模型均入选了温度、湿度和风速等相关要素作为判识因子,入选要素的时次多集中在4:00-8:00区间;(3)独立样本检验表明,基于Logistic回归的霜判识模型对安徽地区霜的平均判识准确率、命中率、漏判率、空判率和TS评分分别为89.0%、91.6%、8.4%、15.8%和78.2%,表明模型对安徽地区的霜具有较好的判识能力;(4)与Bayes判别模型对比发现,基于Logistic回归的霜判识模型在准确率、空判率和TS评分方面表现更优,可以使用Logistic回归模型实现霜的自动化判识。

关键词：霜 Logistic回归自动判识

紫外高级还原技术还原降解水中N-亚硝基二甲胺

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应用化学 2022年第3期39卷 451-460页

作者：查晓松张琳翁元婕冯智梁金苏雯厦门大学嘉庚学院环境科学与工程学院漳州363105 厦门大学嘉庚学院河口生态与环境健康福建省高校重点实验室漳州363105

采用2种高级还原技术(UV/Na_(2)SO_(3)和UV/Na_(2)S_(2)O_(4))还原降解N-亚硝基二甲胺(NDMA),考察pH值、光照强度、还原剂质量浓度和溶解氧等因素对NDMA还原降解效果的影响,计算还原降解反应过程中的表观反应动力学常数,推断NDMA的还原... 详细信息

采用2种高级还原技术(UV/Na_(2)SO_(3)和UV/Na_(2)S_(2)O_(4))还原降解N-亚硝基二甲胺(NDMA),考察pH值、光照强度、还原剂质量浓度和溶解氧等因素对NDMA还原降解效果的影响,计算还原降解反应过程中的表观反应动力学常数,推断NDMA的还原降解机理。研究结果表明,弱酸性条件下有利于2种高级还原技术对NDMA的还原降解。增加光照强度会显著促进高级还原技术对NDMA的还原降解效果,而增加还原剂质量浓度对NDMA去除效果的影响较小。当pH=6.76,光照强度为8.7×10^(-11) E/s,Na_(2)SO_(3)质量浓度为10 mg/L时,经过60 min的反应后,NDMA去除率达到了94.4%。溶解氧会与还原性自由基发生反应,从而抑制了高级还原技术对NDMA的还原降解效果。高级还原技术与NDMA的还原降解反应主要存在3种可能的反应途径:光致水解、光致消去和光氧化。

关键词：紫外高级还原技术 N-亚硝基二甲胺还原降解

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国内外图书馆危机管理研究

天津城市建设学院学报 2010年第1期16卷 69-73页

作者：王广庆金苏文天津城市建设学院图书馆天津300384

阐明了图书馆危机管理的概念,介绍了图书馆所面临的危机类别,论述了国内外图书馆危机管理研究,分析了目前图书馆危机管理研究中存在的一些问题,并指出今后研究的重点,为图书馆危机管理提供参考.

关键词：图书馆危机危机管理危机管理研究