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Study on Process Technology of Gas Bubbling Curtain Substituting Dam in the Tundish of Slab Casting

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Journal of Iron and Steel Research International 2011年第S2期18卷 256-262页

作者： jin You-lin, SHEN Chang, ZHANG Jian-ping, ZHANG Jian (Technology Center of Ma’anshan Iron and Steel Co, Ltd , Ma’anshan 24300, Anhui, China) Maanshan Iron & Steel Co Ltd Ctr Technol Maanshan 24300 Anhui Peoples R China

This paper presents experimental results from physical and mathematical simulation of liquid steel flow in the tundish adopting gas bubbling curtain substituting dam and industrial trials have been investigated based the experimental results. Physical and mathematical simulation results show the gas bubbling curtain can improve pattern of liquid flow in the tundish , accelerate inclusions flotation,and simultaneity metallurgy effect has been further improved when combining weir and turbulence inhibitor. The industrial trial results confirm that content of oxygen , nitrogen and inclusions in continuous casting billets have been reduced when gas bubbling curtain combining weir and turbulence inhibitor.

关键词： gas bubbling curtain physical simulation flow field optimization mathematical simulation

Effect of bonding temperature and heat treatment on microstructure and mechanical property of Mg−6Gd−3Y alloy vacuum diffusion bonded joints

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Transactions of Nonferrous Metals Society of China 2022年第7期32卷 2205-2215页

作者： ping-yi MA Xu CHEN Xing HAN Zhi-qiang luo He-li PENG Shanghai Spaceflight Precision Machinery Institute Shanghai 201600China Shanghai Engineering Technology Research Center of Near-Net Shape Forming for Metallic Materials Shanghai 201600China

Diffusion bonding of as-cast Mg−6Gd−3Y magnesium alloy was carried out at temperatures of 400−480℃ with bonding pressure of 6 MPa for 90 *** bonded joints were solution treated at 495℃ for 14 h and then aged at 200℃ for 30 *** and mechanical properties of joints were *** results showed that rare earth elements and their compounds gathering at bonding interface hindered the grain boundary migration crossing bonding *** strength of as-bonded and as-solution treated joints increased firstly and then decreased with the bonding temperature increasing due to the combined effects of grain coarsening and solid-solution ***-bonded and solution-treated joints fractured at matrix except the joint bonded at 400℃,while aged joints fractured at bonding *** highest ultimate tensile strength of 279 MPa with elongation of 2.8%was found in joint bonded at 440℃ with solution treatment followed by aging treatment.

关键词： Mg−Gd−Y alloy vacuum diffusion bonding post-weld heat treatment bonding interface bonding strength

Identification and epitope mapping of anti-p72 single-chain antibody against African swine fever virus based on phage display antibody library

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农业科学学报:英文版 2023年第9期22卷 2834-2847页

作者： SONG jin-xing WANG Meng-xiang ZHANG Yi-xuan WAN Bo DU Yong-kun ZHUANG Guo-qing LI Zi-bin QIAO Song-lin GENG Rui WU Ya-nan ZHANG Gai-ping College of Veterinary Medicine Henan Agricultural UniversityZhengzhou 450046P.R.China International Joint Research Center of National Animal Immunology Zhengzhou 450046P.R.China College of Animal Science and Veterinary Medicine Tianjin Agricultural UniversityTianjin 300384P.R.China Longhu Laboratory Zhengzhou 450046P.R.China School of Advanced Agricultural Sciences Peking UniversityBeijing 100871P.R.China Henan Provincial Key Laboratory of Animal Immunology Henan Academy of Agricultural SciencesZhengzhou 450002P.R.China

African swine fever virus(ASFV)is a lethal pathogen that causes severe threats to the global swine industry and it has already had catastrophic socio-economic *** date,no licensed prophylactic vaccine *** knowledge exists about the major immunogens of ASFV and the epitope mapping of the key *** such,there is a considerable requirement to understand the functional monoclonal antibodies(mAbs)and the epitope mapping may be of utmost importance in our understanding of immune responses and designing improved vaccines,therapeutics,and *** this study,we generated an ASFV antibody phage-display library from ASFV convalescent swine PBMCs,further screened a specific ASFV major capsid protein(p72)single-chain antibody and fused with an IgG Fc fragment(scFv-83-Fc),which is a specific recognition antibody against ASFV Pig/HLJ/2018 *** the scFv-83-Fc mAb,we selected a conserved epitope peptide(221MTGYKH226)of p72 retrieved from a phage-displayed random peptide ***,flow cytometry and cell uptake experiments demonstrated that the epitope peptide can significantly promote BMDCs maturation in vitro and could be effectively uptaken by DCs,which indicated its potential application in vaccine and diagnostic reagent ***,this study provided a valuable platform for identifying targets for ASFV vaccine development,as well as to facilitate the optimization design of subunit vaccine and diagnostic reagents.

关键词： ASFV phage display antibody library single chain antibody p72 epitope

Real-world investigation of the efficacy and safety of secukinumab for psoriasis treatment in a Chinese population

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Chinese Medical Journal 2021年第1期134卷 117-119页

作者： jing-Xin Zeng Quan luo Ju Wen Xin Tian Xin Zhou Wei Li Ya-ping Tang San-Quan Zhang Wei-Yu Liu Hui-Lan Zhu Xi-Bao Zhang Department of Dermatology Guangdong Second Provincial General HospitalGuangzhouGuangdong 510317China Department of Dermatology Guangzhou Institute of DermatologyGuangzhouGuangdong 510095China

To the Editor:Psoriasis is a chronic inflammatory proliferative disease mediated by T lymphocytes under a multi-gene genetic background.[1]Moreover,its pathogenesis is not fully *** research shows that T cell-mediated immune inflammation plays a key role in the pathophysiology of psoriasis,and its most common downstream mediator,interleukin(IL)-17,is the primary effector cytokine in this *** is a high-affinitive,fully humanized,G1κmonoclonal antibody that can simultaneously block IL-17A from multiple sources.[2]In a global,core,phase III trial,secukinumab was confirmed to rapidly improve psoriasis symptoms;however,the efficacy of its use in Chinese population remains *** study was performed in a Chinese population for a period of 16 weeks to explore the efficacy and safety of secukinumab after its introduction in clinical settings in May 2019.

关键词： psoriasis secukinumab inflammation

高效液相色谱法同时测定烟酰胺单核苷酸中5种有关物质

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食品安全质量检测学报 2024年第1期15卷 182-188页

作者：白玮丽陈俊辰林欣怡张兰平侍慧慧罗穆潮刘梦婷南京医科大学康达学院连云港222000 江苏信佑生物有限公司连云港222000 连云港杰瑞药业有限公司连云港222000

目的构建同时检测烟酰胺单核苷酸(nicotinamide mononucleotide,NMN)中5种有关物质[烟酰胺氧化物、三磷酸腺苷(adenosine triphosphate,ATP)、二磷酸腺苷(adenosine diphosphate,ADP)、一磷酸腺苷(adenosine monophosphate,AMP)及烟酰胺]含量的高效液相色谱法。方法采用C18色谱柱(250 mm×4.6 mm,5μm),以50 mmol/L磷酸二氢钾缓冲盐溶液(用磷酸调节pH为4.5)-甲醇为流动相进行梯度洗脱,流速为1 mL/min,进样量为20μL,检测波长210 nm,柱温20℃,试样盘控温:5℃,并对方法的专属性、灵敏性、准确性、重复性、耐用性及适用性进行验证。结果烟酰胺氧化物、ATP、ADP、AMP及烟酰胺分别在质量浓度0.4060~16.2402、0.4110~16.4403、0.4150~16.6010、0.4163~16.6507、0.4028~16.1206μg/mL范围内,质量浓度与峰面积呈现良好的线性关系,线性系数r^(2)值均大于0.998;加标回收率范围分别为98.5%~102.6%、95.6%~104.1%、98.9%~104.6%、100.0%~102.8%、100.6%~101.3%;3批样品中5种杂质含量检测结果分别为0.18%、0.20%、0.16%、0.14%、0.26%;5种杂质的检出限范围为0.04028~0.04163μg/mL,定量限范围为0.4028~0.4163μg/mL,灵敏度高;专属性、重复性和耐用性均良好。结论该方法简便易行、适用性广,适用于NMN中5种有关物质的检测,为提升其质量标准奠定基础,为食品安全提供保障。

关键词：烟酰胺单核苷酸烟酰胺氧化物三磷酸腺苷二磷酸腺苷一磷酸腺苷烟酰胺高效液相色谱法

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利用红外光谱鉴别饱和脂肪酸及其盐

光谱学与光谱分析 2007年第2期27卷 250-253页

作者：罗曼关平刘文汇北京大学地球与空间科学学院造山带与地壳演化教育部重点实验室北京100871 中国石化石油勘探开发研究院北京100083

有人认为饱和脂肪酸及其盐是碳酸盐岩中的生烃母质,但将它们从天然沉积物中鉴别出来的方法尚不成熟,对分析评价它们在沉积物中的分布造成困难。文章对几种合成饱和脂肪酸及其盐进行红外光谱测试,获得它们的红外吸收光谱。对特征峰进行指... 详细信息

有人认为饱和脂肪酸及其盐是碳酸盐岩中的生烃母质,但将它们从天然沉积物中鉴别出来的方法尚不成熟,对分析评价它们在沉积物中的分布造成困难。文章对几种合成饱和脂肪酸及其盐进行红外光谱测试,获得它们的红外吸收光谱。对特征峰进行指认,分析了饱和脂肪酸及其盐红外光谱的区别。从基团推电子能力、分子折合质量、离子核外电子构型及碳链长度的角度,解释不同饱和脂肪酸以及不同阳离子形成的饱和脂肪酸盐的特征官能团吸收峰波数的变化规律。为地质体中此类物质的鉴别提供参考。

关键词：饱和脂肪酸饱和脂肪酸盐红外光谱分子折合质量推电子能力离子构型长碳链

Synthesis and Structure of the Complexes of 2-Alkoxycarbonylalkyltin Trichlorides with 2-Salicylideneaminophenol

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Chinese Chemical Letters 1998年第2期9卷 219-221页

作者： Lai jin TIAN Bin ZHAO Zheng Yu ZHOU(Department of Chernistry, Qufu Normal University, Qufu 273165)ping YANG Wen Tao YU(Institute of Crystal Materials, Shandong University, jinan 2250100) Department of Chemistry Qufu Normal University Qufu 273165 China Institute of Crystal Materials Shandong University Jinan 2250100 China

The complexes of 2-Alkoxycarbonylalkyltin Trichlorides with 2-Salicylideneamino-phenol have been synthesized and characterized by means of elemental analysis, lR, 1H-NMR andX-ray single crystal diffraction. The struct... 详细信息

关键词： Complex 2-Alkoxycarbonylalkyltin Trichlorides 2-Salicylideneaminophenol

基于机器学习鉴定支气管肺发育不良相关基因及免疫浸润分析

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中华生物医学工程杂志 2024年第1期30卷 16-23页

作者：胡诤贇江军飞田坪娄泽圆赵志金李小东罗星王永丹徐礼贵上海市松江区中心医院儿科，上海201600 上海市松江区中心医院药剂科，上海201600 云南省昭通市昭阳区中医医院儿科，昭通657000

目的对支气管肺发育不良(BPD)密切相关的基因进行鉴定,为揭示BPD发病机制提供理论依据。方法从基因表达综合数据库(GEO)获取BPD患儿的血标本数据集GSE32472基因微阵列,筛选出差异表达基因并行CIBERSORT反卷积算法免疫浸润分析,再利用加... 详细信息

目的对支气管肺发育不良(BPD)密切相关的基因进行鉴定,为揭示BPD发病机制提供理论依据。方法从基因表达综合数据库(GEO)获取BPD患儿的血标本数据集GSE32472基因微阵列,筛选出差异表达基因并行CIBERSORT反卷积算法免疫浸润分析,再利用加权基因共表达网络分析(WGCNA)筛选出关键颜色模块基因集并与差异表达基因比对后得出交集基因。通过LASSO回归依据lambda惩罚值计算出每个基因系数,筛选出BPD相关的候选基因。基于风险评分中位值构建的高低分险组模型,利用受试者工作特征(ROC)曲线和多因素Logistic回归分析验证候选基因与BPD的关系,并在外部数据集GSE108756中验证诊断效能。结果共筛选出291个差异表达基因,免疫浸润分析揭示了BPD与中性粒细胞、激活CD8^(+)T细胞、效应记忆CD8^(+)T细胞、中央记忆CD4^(+)T细胞以及树突状细胞的激活密切相关。WGCNA分析确定GSE32472中与BPD最密切相关的品红色模块包含169个基因,这些与差异表达基因交集后获得的32个基因通过LASSO回归筛选并建模,最终得出的5个候选基因:UBASH3A、TCF7、CD3E、CD3D、CD6对诊断BPD具有较好的特异性和敏感性[曲线下面积(AUC)为0.825]。多因素Logistic回归分析结果显示,UBASH3A、TCF7、CD3E、CD6对诊断BPD的诊断差异具有统计学意义(均P0.7。结论通过机器学习算法筛选出的5个候选基因,可能成为潜在的BPD基因生物标志物,为深入探索BPD的发病机理和潜在治疗靶点提供了理论基础。

关键词：免疫浸润加权基因共表达网络 LASSO回归支气管肺发育不良候选基因

Change in drug resistance of staphylococcus aureus

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中国应用生理学杂志 2013年第6期29卷 573-576页

作者： Yan LIN Yan LIU Yan-ping luo Chang-ting LIU Department of Respiratory Medicine of Nanlou the General Hospital of PLA Department of Microbiology the General Hospital of PLA

Objective To analyze the change in drug resistance of staphylococcus aureus(SAU) in the PLA general hospital from January 2008 to December 2012, and to provide solid evidence to support the rational use of antibiotics for clinical applications. Methods The SAU strains isolated from clinical samples in the hospital were collected and subjected to the Kirby-Bauer disk diffusion test. The results were assessed based on the 2002 American National Committee for Clinical Laboratory Standards(NCCLS) guidelines. Results SAU strains were mainly isolated from sputum, urine, blood and wound excreta and distributed in penology, neurology wards, orthopedics and surgery ICU wards. Except for glycopeptide drugs, methicillinresistant staphylococcus aureus(MRSA) had a higher drug resistance rate than those of the other drugs and had significantly more resistance than methicillin-sensitive staphylococcus aureus(MSSA)(P<0.05). In the dynamic observation of drug resistance, we discovered a gradual increase in drug resistance to fourteen test drugs during the last five years. Conclusion Drug resistance rate of SAU stayed at a higher level over the last five years; moreover, the detection ratio of MRSA keeps rising year by year. It is crucial for physicians to use antibiotics rationally and monitor the change in drug resistance in a dynamic way.

关键词：金黄色葡萄球菌耐药性 ICU病房合理使用肽类药物 MRSA 临床实验室 SAU