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Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

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World Journal of Gastroenterology 2017年第2期23卷 224-231页

作者： Hong-Bo Shi jin-li lou Hong-lin Shi Feng Ren Yu Chen Zhong-Ping Duan Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical University Clinical Laboratory Center Beijing Youan HospitalCapital Medical University Artificial liver Center Beijing Youan HospitalCapital Medical University

AIM To prepare a Gpm6a/Reelin^(GFPCreERT2) construct with a rapid and reliable strategy using a bacterial artificial chromosome(BAC). METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreE RT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion. Recombinant clones were transformed into SW102 E. coli to remove the kanamycin *** A complete BAC was successfully transformed into SW102 E. coli by electroporation because BAC purified from SW102 E. coli showed the same pattern as the original BAC with Bam H I digestion. The GFPCre ERT2 fragment was deemed to have been prepared successfully because we obtained the same size fragment as expected. Homologous recombination was induced, and GFPCre ERT2 was deemed to have been inserted into the correct site of the BAC because we found the band change was the same as the expected pattern after restriction enzyme digestion. The kanamycin unit was deemed to have been removed successfully because we obtained different sizes of bands that were consistent with the results expected by PCR with different primers. CONCLUSION The construct of Gpm6 a^(GFPCreERT2) or Reelin^(GFPCreERT2) was prepared successfully, which will establish a foundation for tracing the hepatic stellate cell lineage and studying its function.

关键词： Bacterial artificial chromosome Homologous recombination Glycoprotein M6a Reelin

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Retraction Note: Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

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World Journal of Gastroenterology 2019年第25期25卷 3281-3282页

作者： Hong-Bo Shi jin-li lou Hong-lin Shi Feng Ren Yu Chen Zhong-Ping Duan Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical UniversityBeijing 100069China Clinical Laboratory Center Beijing Youan HospitalCapital Medical UniversityBeijing 100069China Artificial liver Center Beijing Youan HospitalCapital Medical UniversityBeijing 100069China

We have decided to retract the above article for further consideration due to some misunderstandings in communication.

关键词： Retraction note

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The Change and Significance of Regulatory T Cells in Mice liver Fibrosis

The Change and Significance of Regulatory T Cells in Mice Li...

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中华医学会第十六次全国病毒性肝炎及肝病学术会议

作者： Xin liu jin-li lou li Bai Xiao-hui Zhang Yu Chen Zhong-ping Duan Clinical Laboratory Center Beijing You'an HospitalCapital Medical University Artificial liver Center Beijing You'an HospitalCapital Medical University

Objective To investigate the changes and significances of CD4 + CD25 + Foxp3 + regulatory T lymphocytes (Tregs ) in the peripheral blood and liver-infiltrating lymphocytes(liLs) in liver fibrosis of *** C57BL/6 mice were randomly divided into the model group and the control *** mice of liver fibrosis were induced by intraperitoneal injection of carbon tetrachloride(CC14),at the same time,the control

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Autophagy in anti-apoptotic effect of augmenter of liver regeneration in HepG2 cells

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World Journal of Gastroenterology 2015年第17期21卷 5250-5258页

作者： Hong-Bo Shi Hai-Qing Sun Hong-lin Shi Feng Ren Yu Chen De-Xi Chen jin-li lou Zhong-Ping Duan Beijing Youan Hospital Capital Medical University Beijing Institute of Hepatology

AIM:To investigate the role of autophagy in the antiapoptotic effect of augmenter of liver regeneration(ALR).METHODS:Autophagy was induced through serum *** ALR-expressing plasmid was transfected into HepG2 cells,and autophagic flux was determined using fluorescence microscopy,electron microscopy,Western blot and quantitative polymerase chain reaction(q PCR) *** ALR-expressing plasmid transfection,an autophagy inhibitor [3-methyladenine(3-MA)] was added to HepG2 cells,and apoptosis was observed using fluorescence microscopy and flow ***:Autophagy was activated in HepG2 cells,peaking at 24 h after serum *** protein light chain three-II levels were higher in HepG2 cells treated with ALR than in control cells,fluorescence microscopy,electron microscopy and q PCR studies showed the similar trend,and p62 levels showed the opposite trend,which indicated that ALR may play an important role in increasing autophagy *** numbers of apoptotic cells were substantially higher in HepG2 cells treated with both ALR and 3-MA than in cells treated with ALR ***,the protective effect of ALR was significantly attenuated or abolished when autophagy was inhibited,indicating that the anti-apoptotic effect of ALR may be related to ***:ALR protects cells from apoptosis partly through increased autophagy in HepG2 cells and may be valuable as a new therapeutic treatment for liver disease.

关键词： Autophagy Augmenter of liver regeneration Apoptosis HepG2 cells

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Preparation of silver nanoparticles through the reduction of straw-extracted lignin and its antibacterial hydrogel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 504-514页

作者： lou Zhang Shuo li Fu Tang jingkai Zhang Yuetong Kang Hean Zhang lidong li State Key Laboratory for Advanced Metals and Materials School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083China

Silver nanoparticles(Ag NPs)have attracted attention in the field of biomaterials due to their excellent antibacterial ***,the reducing and stabilizing agents used for the chemical reduction of Ag NPs are usually toxic and may cause water *** this work,Ag NPs(31.2 nm in diameter)were prepared using the extract of straw,an agricultural waste,as the reducing and stabilizing *** analysis revealed that the straw extract contained lignin,the structure of which possesses phenolic hydroxyl and methoxy groups that facilitate the reduction of silver salts into Ag *** surfaces of Ag NPs were negatively charged due to the encapsulation of a thin layer of lignin molecules that prevented their *** the prepared Ag NPs were added to the precursor solution of acrylamide,free radical polymerization was triggered without the need for extra heating or light irradiation,resulting in the rapid formation of an Ag NP-polyacrylamide composite *** inhibition zone test proved that the composite hydrogel possessed excellent antibacterial ability due to the presence of Ag *** prepared hydrogel may have potential applications in the fabrication of biomedical materials,such as antibacterial dressings.

关键词： silver nanoparticles hydrogel straw extraction antibacterial

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介孔ZSM-22沸石负载Pt对十二烷加氢异构化性能的影响

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现代化工 2019年第2期39卷 74-78页

作者：李金楼赵文博傅雯倩常州大学石油化工学院江苏常州213164

以Al_2(SO_4)_3·18H_2O为铝源、硅溶胶为硅源、有机硅季铵盐为介孔模板剂,通过水热晶化方法成功制备了具有较大介孔孔容和较高外表面积的介孔ZSM-22沸石(ZSM-22-M)。利用XRD、N2吸附-脱附、SEM和NH3-TPD等手段对ZSM-22-M沸石进行表征... 详细信息

以Al_2(SO_4)_3·18H_2O为铝源、硅溶胶为硅源、有机硅季铵盐为介孔模板剂,通过水热晶化方法成功制备了具有较大介孔孔容和较高外表面积的介孔ZSM-22沸石(ZSM-22-M)。利用XRD、N2吸附-脱附、SEM和NH3-TPD等手段对ZSM-22-M沸石进行表征。研究了ZSM-22-M和微孔ZSM-22沸石负载Pt催化剂(Pt/HZSM-22-M和Pt/HZSM-22)对正十二烷加氢异构化性能的影响,结果表明,Pt/HZSM-22-M具有非常优异的加氢异构化性能。Pt/HZSM-22-M上具有相互匹配的金属中心-酸中心数目,有利于提高其异构化选择性,同时其上丰富的介孔结构促进了反应分子的扩散。

关键词：介孔ZSM-22 Pt催化剂加氢异构正十二烷

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不同病程的慢性乙型肝炎病毒感染者外周血中调节性T细胞和自然杀伤细胞的表达及相关性

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世界华人消化杂志 2016年第12期24卷 1885-1890页

作者：张晓慧娄金丽段钟平陈煜首都医科大学附属北京佑安医院人工肝中心北京市100069 首都医科大学附属北京佑安医院临检中心北京市100069

目的:观察乙型肝炎病毒(hepatitis B virus,HBV)慢性感染者外周血中调节性T细胞(regulatory T cells,Tregs)和自然杀伤(natural killer,NK)细胞的表达及相关性.方法:选取HBV慢性感染患者共96例,其中慢性乙型肝炎患者42例,乙型肝炎肝硬... 详细信息

目的:观察乙型肝炎病毒(hepatitis B virus,HBV)慢性感染者外周血中调节性T细胞(regulatory T cells,Tregs)和自然杀伤(natural killer,NK)细胞的表达及相关性.方法:选取HBV慢性感染患者共96例,其中慢性乙型肝炎患者42例,乙型肝炎肝硬化患者30例,HBV携带者24例,抽取外周血用流式细胞分析技术检测其Tregs和NK细胞的表达情况.同时选取健康者21例作为对照组.结果:HBV慢性感染者外周血CD4+CD25+Foxp3+Tregs占CD4+T细胞的比例整体高于健康对照组,其中乙型肝炎肝硬化患者外周血CD4+CD25+Foxp3+Tregs占CD4+T细胞的比例最高,其次为慢性乙型肝炎患者,最后为HBV携带者和健康对照组.相反,NK细胞占淋巴细胞的比例在HBV慢性感染者外周血中整体较健康对照组低,其中乙型肝炎肝硬化组表达最低,其次为慢性乙型肝炎组,最后为HBV携带者,但都低于健康对照组.进一步分析显示NK细胞的表达与Tregs呈负相关性(r=-0.3280,P<0.05).结论:不同阶段的HBV慢性感染患者外周血中Tregs和NK细胞表达不同,且两者呈明显的负相关性.

关键词：调节性T细胞自然杀伤细胞乙型肝炎病毒慢性感染

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针药结合治疗对急性脑梗死患者生活质量的影响

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临床医学 2015年第9期35卷 125-126页

作者：李金楼杨梅云河南省林州市人民医院林州456550

目的观察针药结合治疗对急性脑梗死患者神经功能及生活能力的影响。方法将162例急性脑梗死患者随机分为两组,对照组80例,给予西医常规治疗;治疗组82例,在对照组治疗基础上加针药结合治疗。比较两组治疗前后神经功能缺损评分及患者日常... 详细信息

目的观察针药结合治疗对急性脑梗死患者神经功能及生活能力的影响。方法将162例急性脑梗死患者随机分为两组,对照组80例,给予西医常规治疗;治疗组82例,在对照组治疗基础上加针药结合治疗。比较两组治疗前后神经功能缺损评分及患者日常生活能力变化情况,统计临床疗效。结果治疗组基本治愈率+显著进步率为82.93%,对照组为68.75%,差异有统计学意义(P<0.05),治疗组临床疗效优于对照组。两组治疗后神经功能缺损评分与本组治疗前比较明显下降(P<0.05),生活能力评分提高(P<0.05),且两组治疗后组间比较差异均有统计学意义(P<0.05)。结论针药结合治疗可明显提高急性脑梗死患者的生活质量。

关键词：脑梗死针药结合治疗生活质量

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超音速流动条件下Sn/N2O(O2)化学发光反应动力学的实验研究

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物理化学学报 1986年第2期2卷 146-152页

作者：罗静远方尔梯崔学义金朝鹏李学初楼南泉中国科学院大连化学物理研究所

在温度低于900K 和1—10乇腔压范围内,首次采用等离子加热方法产生金属 Sn 蒸气,并利用超音速流动反应装置,研究了载气为 N和 N+Ar 时 Sn+NO 和 Sn+O的化学发光反应。首次采用光学多通道分析仪(OMA Model 1450)获得了不同腔压下 SnO(a~3... 详细信息

在温度低于900K 和1—10乇腔压范围内,首次采用等离子加热方法产生金属 Sn 蒸气,并利用超音速流动反应装置,研究了载气为 N和 N+Ar 时 Sn+NO 和 Sn+O的化学发光反应。首次采用光学多通道分析仪(OMA Model 1450)获得了不同腔压下 SnO(a~3∑~+----X~1∑~+)跃迁的化学发光光谱,得出了不同腔压下 SnO(a~3∑~+)态的相对振动布居,并将本实验结果与低亚音速高温快速流动反应器(HTFFR)中所得的结果做了比较。

关键词：超音速流动化学发光反应超音速流可压缩流动 O2 Sn/N2O 动力学

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Smart Cellulose‑Based Janus Fabrics with Switchable liquid Transportation for Personal Moisture and Thermal Management

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Nano-Micro Letters 2025年第1期17卷 333-347页

作者： Jianfeng Xi Yanling lou liucheng Meng Chao Deng Youlu Chu Zhaoyang Xu Huining Xiao Weibing Wu Jiangsu Co‑Innovation Center of Efficient Processing and Utilization of Forest Resources International Innovation Center for Forest Chemicals and MaterialsJiangsu Provincial Key Lab of Sustainable Pulp and Paper Technology and Biomass MaterialsNanjing Forestry UniversityNanjing 210037People’s Republic of China College of Materials Science and Engineering Nanjing Forestry UniversityNanjing 210037People’s Republic of China Department of Chemical Engineering University of New BrunswickFrederictonNB E3B 5A3Canada Macromolecular Chemistry and Bavarian Polymer Institute University of BayreuthBayreuth 95440Germany

The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human ***,the development of smart and dynamic fabrics capable of managing personal moisture/thermal comfort in response to changing external environments remains a ***,a smart cellulose-based Janus fabric was designed to dynamically manage personal moisture/*** cotton fabric was grafted with N-isopropylacrylamide to construct a temperature-stimulated transport ***,hydrophobic ethyl cellulose and hydrophilic cellulose nanofiber were sprayed on the bottom and top sides of the fabric to obtain wettability *** fabric exhibits anti-gravity directional liquid transportation from hydrophobic side to hydrophilic side,and can dynamically and continuously control the transportation time in a wide range of 3–66 s as the temperature increases from 10 to 40℃.This smart fabric can quickly dissipate heat at high temperatures,while at low temperatures,it can slow down the heat dissipation rate and prevent the human from becoming too *** addition,the fabric has UV shielding and photodynamic antibacterial properties through depositing graphitic carbon nitride nanosheets on the hydrophilic *** smart fabric offers an innovative approach to maximizing personal comfort in environments with significant temperature variations.

关键词： Directional water transport Cotton fabric Anti-gravity directional liquid transportation Janus wettability

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