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Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells

World Journal of Gastroenterology 2016年第44期22卷 9752-9764页

作者： Masahiro Yamamoto Mitsue Nishiyama Seiichi Iizuka Shigeaki Suzuki Norihiro Suzuki Sadakazu Aiso jin nakahara Tsumura Research Laboratories Tsumura & Co. Department of Neurology Keio University School of Medicine Department of Anatomy Keio University School of Medicine

AIM To investigate the possible involvement of transient receptor potential vanilloid 1(TRPV1) in maturation of enteric glial cells(EGCs).METHODS Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus(MP) as well as cultured MP cells and EGCs using TRPV1 knockout(KO) *** We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type(WT) but not KO mice. Expression of glial fibrillary acidic protein(GFAP) immunoreactive signals was lower at postnatal day(PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100 B, another EGC/astrocyte-specific marker. CONCLUSION These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility.

关键词： Enteric glial cells Enteric nervous system Glial fibrillary acidic protein S100B Smooth muscle cells

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A non-alternate 3D structure and its practical security evaluation against differential and linear cryptanalysis

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Science China(Information Sciences) 2018年第5期61卷 306-308页

作者： Qian WANG Chenhui jin Information Science and Technology Institute

The building blocks of the original 3D block cipher structure,first proposed by nakahara[1]and generalized in[2],are four different invertible transformations that are similar to AES(advanced encryption standard[3])but operate in a 3D array A:RoundK ey Additionκi;Byte Substitutionγ;ShiftR owsθ1,which are applied in alternate rounds;and Mix Columnsπ.Owing to the alternate application of two different ShiftR ows transformationsθ1,the 3D block cipher[1]achieves quick diffusion and guarantees enough active S-boxes to resist differential and linear cryptanalysis[4].

关键词： A non-alternate 3D structure and its practical security evaluation against differential and linear cryptanalysis

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