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The role of extracellular vesicles in animal reproduction and diseases

Journal of Animal Science and Biotechnology 2022年第6期13卷 1634-1656页

作者： Sangiliyandi Gurunathan Min-Hee Kang Hyuk Song Nam Hyung kim jin-hoi kim Department of Stem Cell and Regenerative Biotechnology Konkuk UniversitySeoul 05029Korea Guangdong Provincial Key Laboratory of Large Animal models for Biomedicine Wuyi UniversityJiangmen 529020China

Extracellular vesicles(EVs)are nanosized membrane-enclosed compartments that serve as messengers in cell-to-cell communication,both in normal physiology and in pathological *** can transfer functional proteins and genetic information to alter the phenotype and function of recipient cells,which undergo different changes that positively affect their structural and functional *** fluids are enriched with several subpopulations of EVs,including exosomes,microvesicles(MVs),and apoptotic bodies carrying several cargoes,such as lipids,proteins,and nucleic *** associated with the reproductive system are actively involved in the regulation of different physiological events,including gamete maturation,fertilization,and embryo and fetal *** can influence follicle development,oocyte maturation,embryo production,and endometrial-conceptus *** loaded with cargoes are used to diagnose various diseases,including pregnancy disorders;however,these are dependent on the type of cell of origin and pathological ***-derived microRNAs(miRNAs)and proteins in the placenta regulate inflammatory responses and trophoblast invasion through intercellular delivery in the placental *** review presents evidence regarding the types of extracellular vesicles,and general aspects of isolation,purification,and characterization of EVs,particularly from various types of ***,we discuss EVs as mediators and messengers in reproductive biology,the effects of EVs on placentation and pregnancy disorders,the role of EVs in animal reproduction,in the male reproductive system,and mother and embryo *** addition,we emphasize the role of microRNAs in embryo implantation and the role of EVs in reproductive and therapeutic ***,we discuss the future perspectives of EVs in reproductive biology.

关键词： Embryos Extracellular vesicles Implantation MicroRNA Pregnancy disorders

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A highly efficient method for enriching TALEN or CRISPR/Cas9-edited mutant cells

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Journal of Genetics and Genomics 2016年第12期43卷 705-708页

作者： Hideyo Yasuda Eunsu kim Abu Musa Md Talimur Reza jin-hoi kim Department of Stem Cell and Regenerative Biology Konkuk University

In recent years,genome editing with site-specific nucleases,such as ZFN（zinc finger nuclease）,TALEN（transcription activatorlike effector nucleases）,and CRISPR/Cas9（the type II bacterial clustered,regularly interspaced,short palindromic repeats-associated protein 9）,has gained popularity for use in cell lines,animals,and plants（Urnov et al.,2010;Miller et al.,2011;Cong et al.

关键词： A highly efficient method for enriching TALEN or CRISPR/Cas9-edited mutant cells gene DNA RNA ZFN

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Interstitial tissue-specific gene expression in mouse testis by intra-tunica albuguineal injection of recombinant baculovirus

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Asian Journal of Andrology 2009年第3期11卷 342-350页

作者： Hyun Jung Park Won Young Lee jin hoi kim Jae Hwan kim Hun Jong Jung Nam Hyung kim Bo Kyung kim Hyuk Song Department of Animal Science College of Natural Science Konkuk University Chung-ju 380-701 Korea CHA Stem Cell Institute Graduate School of Life Science and Biotechnology Pochon CHA University Seoul 135-907 Korea Department of Animal Biotechnology College of Animal Biotechnology Konkuk University Seoul 143-701 Korea Occupation and Environment Department Medical School Konkuk University Chung-ju 380-704 Korea Department of Animal Science College of Agriculture Chungbuk National University Choung-ju Chung-buk 361-763 Korea Department of Physiology Medical School Konkuk University Chung-ju 380-701 Korea

The purpose of this study is to establish a gene delivery system for interstitial tissue-specific protein expression in mice testes using modified recombinant baculovirus. Green fluorescent protein （GFP）-expressing recombinant baculovirus （GFP-baculovirus）, in which the insect cell-specific polyhedron promoter was replaced by the cytomegalovirus （CMV）-IE promoter, was used to transfect testicular cells in vitro, and for intra-tunica albuguineal injection of the interstitial tissue of the testis. GFP expression was monitored in frozen testes sections by fluorescence microscopy. Expression of GFP in testicular tissues was also assessed by reverse transcription polymerase chain reaction （RT-PCR）, and protein expression was assessed by Western blot. Testicular cells in vitro were infected efficiently by modified recombinant GFP-baculovirus. lntra-tunica albuguineal injection of GFP- baculovirus into the mouse testis resulted in a high level of GFP expression in the interstitial tissues. RT-PCR analysis clearly showed GFP gene expression in the testis, particularly interstitial tissues. Intra-tunica albuguineal injection of a modified baculovirus that encoded recombinant rat insulin-like growth factor binding protein （IGFBP）-5 resulted in an increase in IGFBP-5 in testis and semen. In conclusion, we have developed an efficient delivery system for gene expression in vivo in testicular cells, particularly cells of the interstitial tissue using intratunica albuguineal injection of a modified recombinant baculovirus. This method will be particularly relevant for application that requires gene delivery and protein expression in the testicular cells of the outer seminiferous tubule of the testis.

关键词： intra-tunica albuguineal injection recombinant baculovirus testis gene delivery

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THE CYTOPLASM OF MOUSE GERMINAL VESICLE （GV） STAGE OOCYTES CAN ENHANCE SOMATIC CELL NUCLEAR REPROGRAMMING

THE CYTOPLASM OF MOUSE GERMINAL VESICLE （GV） STAGE OOCYTES...

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The 5th Annual Conference of the Asian Reproductive Biotechnology Society

作者： Hong-Thuy BUI Sayaka WAKAYAMA Satoshi KISHIGAMI jin-hoi kim Nguyen Van THUAN Teruhiko WAKAYAMA Center for Developmental Biology RIKEN Kobe2-2-3 Minatojima-minamimachiKobe 6500047Japan Department of Animal Biotechnology College of Animal Bioscience & BiotechnologyKONKUK University1 Hwayang-dongGwangjin-guSeoul 143-701Korea

＜正＞In mammalian cloning,evidence suggests that genomic reprogramming factors are located in the nucleus rather than the cytoplasm of oocytes or ***,little is known about the mechanisms,and new methods using nuclear...

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Enabling Digital Earth simulation models using cloud computing or grid computing-two approaches supporting high-performance GIS simulation frameworks

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International Journal of Digital Earth 2013年第4期6卷 383-403页

作者： Ick-hoi kim Ming-Hsiang Tsou Department of Geography San Diego State UniversitySan DiegoCAUSA

Geospatial simulation models can help us understand the dynamic aspects of Digital *** implement high-performance simulation models for complex geospatial problems,grid computing and cloud computing are two promising computational *** research compares the benefits and drawbacks of both in Web-based frameworks by testing a parallel Geographic Information System(GIS)simulation model(Schelling’s residential segregation model).The parallel GIS simulation model was tested on XSEDE(a representative grid computing platform)and Amazon EC2(a representative cloud computing platform).The test results demonstrate that cloud computing platforms can provide almost the same parallel computing capability as high-end grid computing ***,cloud computing resources are more accessible to individual scientists,easier to request and set up,and have more scalable software architecture for on-demand and dedicated Web *** advantages may attract more geospatial scientists to utilize cloud computing for the development of Digital Earth simulation models in the future.

关键词： grid computing cloud computing parallel computing simulation model geospatial cyberinfrastructure

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Solid-state phase transformations toward a metal-organic framework of 7-connected Zn_(4)0 secondary building units

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Nano Research 2021年第2期14卷 411-416页

作者： Jaehui kim Junsu Ha Jae Hwa Lee hoi Ri Moon Department of Chemistry Ulsan National Institute of Science and Technology(UNIST) Ulsan 44919Republic of Korea

In the development of metal-organic frameworks(MOFs),secondary building units(SBUs)have been utilized as molecular modules for the construction of nanoporous materials with robust *** solvothermal synthetic conditions,dynamic changes in the metal coordination environments and ligand coordination modes of SBUs determine the resultant product ***,MOF phases with new topologies can also be achieved by post-synthetic treatment of as-synthesized MOFs via the introduction of acidic or basic moieties that cause the simultaneous cleavage/reformation of coordination bonds in the solid *** this sense,we studied the solid-state transformation of two ndc-based Zn-MOFs(ndc=1,4-naphthalene dicarboxylate)with different SBUs but the same pcu topology to another MOF with sev *** of the chosen MOFs with pcu nets is[Zn_(2)(ndc)_(2)(bpy)]_(n)(bpy=4,4,-bipyridine),(6C_(bpy)-MOF)consisting of a 6-connected pillared-paddlewheel SBU,and the other is IRMOF-7 composed of 6-connected Zn40(C 00)6 SBUs and *** post-structural modification,these pcu MOFs were converted into the same MOF with sev topology constructed from the uncommon 7-connected Zri_(4)O(COO)_(7)SBU(7C-MOF).The appropriate post-synthetic conditions for the transformation of each SBUs were systematically *** addition,the effect of the pillar molecules in the pillared-paddlewheel MOFs on the topology conversion was studied in terms of the linker basicity,which determines the inertness during the solid-state phase *** post-synthetic modification approach is expected to expand the available methods for designing and synthesizing MOFs with controlled topologies.

关键词： metal-organic framework secondary building units solid-state transform ation linker basicity ligand addition reaction

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Fluoropolymer-based organic memristor with multifunctionality for flexible neural network system

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npj Flexible Electronics 2021年第1期5卷 296-303页

作者： Min-Hwi kim Hea-Lim Park Min-hoi kim Jaewon Jang jin-Hyuk Bae In Man Kang Sin-Hyung Lee School of Electrical and Computer Engineering Seoul National University1 Gwanak-roGwanak-guSeoul 08826Republic of Korea Department of Materials Science and Engineering Seoul National University of Science and TechnologySeoul 01811Republic of Korea Department of Creative Convergence Engineering Hanbat National UniversityYuseong-guDaejeon 305-719Republic of Korea School of Electronics Engineering and School of Electronic and Electrical EngineeringKyungpook National University80 Daehak-roBuk-guDaegu 702-701Republic of Korea

In this study,we propose an effective strategy for achieving the flexible one organic transistor–one organic memristor(1T–1R)synapse using the multifunctional organic *** dynamics of the conductive nanofilament(CF)in a hydrophobic fluoropolymer medium is explored and a hydrophobic fluoropolymer-based organic memristor is *** flexible 1T–1R synapse can be fabricated using the solution process because the hydrophobic fluorinated polymer layer is produced on the organic transistor without degradation of the underlying *** developed flexible synapse exhibits multilevel conductance with high reliability and stability because of the fluoropolymer film,which acts as a medium for CF growth and an encapsulating layer for the organic ***,the synapse cell shows potential for high-density memory systems and practical neural *** effective concept for developing practical flexible neural networks would be a basic platform to realize the smart wearable electronics.

关键词： transistor network neural

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Influence of Tropical Cyclone Landfalls on Spatiotemporal Variations in Typhoon Season Rainfall over South China

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Advances in Atmospheric Sciences 2010年第2期27卷 443-454页

作者： Min-Hee LEE Chang-hoi HO Joo-Hong kim Computational Science and Technology Seoul National University School of Earth and Environmental Sciences Seoul National University Met Office Hadley Centre

This study examined the impact of tropical cyclone (TC) landfalls on the spatiotemporal variations in the rainfall over South China for the period 1957–2005. The target region was selected to show the noteworthy contribution of TC landfalls to the total rainfall during the typhoon season (July–October). Two prevailing spatial variations in the rainfall were obtained from an EOF analysis. The first EOF mode displays singlesign variability over South China with an explained variance of 23.4%. The associated time series of this mode fluctuates on a decadal timescale and was found to be correlated with TC genesis in the South China Sea. The second EOF mode shows a seesaw pattern between Hainan Island/Guangdong Province and the remaining regions with an explained variance of 11.4%. This seesaw pattern results from an anti-correlation in seasonal TC landfalls between the two regions, which was found in previous studies. This is related to the strengthening (weakening) of the upper tropospheric jets and the corresponding development of a massive anticyclonic (cyclonic) circulation over East Asia. The EOF analysis was also conducted using just the data for rainfall caused by landfalling TCs. This revealed that the first EOF mode using just the TC-induced rainfall is nearly identical to the second mode from the total rainfall. The obvious seesaw pattern of the first mode when employing just the TC-induced rainfall in the EOF analysis implies that this pattern has larger temporal variability than the single-signed pattern (i.e., the first EOF mode using the total rainfall) in terms of TC landfalls. This study suggests that TC landfalls over South China and the accompanying rainfall significantly modulate the spatial variation of the typhoon season rainfall there.

关键词： rainfall tropical cyclone South China typhoon season

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Association of chronic viral hepatitis B with insulin resistance

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World Journal of Gastroenterology 2012年第42期18卷 6120-6126页

作者： Jeong Gyu Lee Sangyeoup Lee Yun jin kim Byung Mann Cho Joo Sung Park Hyung hoi kim JaeHun Cheong Dong Wook Jeong Yu Hyun Lee Young Hye Cho Mi jin Bae Eun Jung Choi Department of Family Medicine Pusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Family Medicine Medical Research InstitutePusan National University HospitalBusan 602-739South Korea Medical Education Unit Pusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Family Medicine Clinic Yangsan Pusan National University HospitalBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Preventive Medicine and Occupational Medicine Pusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Family Medicine Dong-A University Medical CenterBusan 602-715South Korea Department of Laboratory Medicine Biomedical Informatics UnitPusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Molecular Biology College of Natural SciencesPusan National UniversityBusan 602-739South Korea

AIM:To investigate the relationship between chronic viral hepatitis B(CVHB) and insulin resistance(IR) in Korean ***:A total of 7880 adults(3851 men,4029 women) who underwent a comprehensive medical examination were enrolled in this *** diagnosed with either diabetes mellitus,or any other disorder that could influence their insulin sensitivity,were ***,metabolic risk factors,hepatitis B surface antigen,hepatitis B surface antibody,hepatitis B core antibody,fasting plasma glucose and insulin were measured for all *** model assessment(HOMA),quantitative insulin check index(QUICKI),and Mf fm index were used for determining insulin *** participant was categorized into a negative,recovery,or CVHB *** compare variables between groups,a t-test and/or one-way analysis of variance were *** correlation coefficients were computed to present the association between insulin resistance and other *** logistic regression analysis was used to assess the independent association between CVHB and ***:The mean age of men and women were 48.9 and 48.6 years,*** in the CVHB group had significantly higher waist circumference [(86.0 ± 7.7 cm vs 87.3 ± 7.8 cm,P = 0.004 in men),(78.3 ± 8.6 cm vs 80.5 ± 8.5 cm,P < 0.001 in women)],cystatin C [(0.96 ± 0.15 mg/dL vs 1.02 ± 0.22 mg/dL,P < 0.001 in men),(0.84 ± 0.15 mg/dL vs 0.90 ± 0.16 mg/dL,P < 0.001 in women)],fasting insulin [(5.47 ± 3.38 U/mL vs 6.12 ± 4.62 U/mL,P < 0.001 in men),(4.57 ± 2.82 U/mL vs 5.06 ± 3.10 U/mL,P < 0.001 in women)] and HOMA index [(1.24 ± 0.86 vs 1.43 ± 1.24,P < 0.001 in men),(1.02 ± 0.76 vs 1.13 ± 0.87,P = 0.033 in women)] compared to control *** HOMA index revealed a positive correlation with body mass index(BMI)(r = 0.378,P < 0.001),waist circumference(r =0.356,P < 0.001),percent body fat(r = 0.296,P < 0.001),systolic blood pressure(r = 0.202,P < 0.001),total cholesterol(r = 0.134,P < 0.00

关键词：胰岛素抵抗病毒性肝炎慢性 Logistic回归分析协会胰岛素敏感性单向方差分析表面抗原

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Characteristics of Atmospheric Circulation Associated with Cold Surge Occurrences in East Asia:A Case Study During 2005/06 Winter

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Advances in Atmospheric Sciences 2008年第5期25卷 791-804页

作者： Tae-Won PARK Jee-Hoon JEONG Chang-hoi HO Seong-Joong kim School of Earth and Environmental Sciences Seoul National University Seoul Korea Department of Earth Sciences University of Gothenburg Gothenburg Sweden Korea Polar Research Institute Incheon Korea

The characteristics of the upper-level circulation and thermodynamical properties for the period when two distinct cold surges broke out over East Asia during the 2005/06 winter are investigated. From early December 2005 to early January 2006, exceptionally cold weather lasted for approximately one month due to two successive cold surges that took place on 2 December 2005 and 2 January 2006, respectively. This study reveals that both involve the upper-tropospheric circulation, which induces the amplification and expansion of the surface Siberian high toward East Asia, but arose from different causes： the former is caused by the upper-level blocking originated from the North Pacific and the latter is caused by the upper-level wave train across the Eurasian Continent. In particular, it is suggested that the lower-tropospheric anomalous wind caused by upper-level circulation anomalies and a steep meridional temperature gradient amplified by phase-locked annual cycle combined to induce very strong cold advection in East Asia, which resulted in exceptionally cold weather that lasted for several weeks. The present results emphasize that the characteristics of the upper-tropospheric circulation can be considered as important precursors to cold surge occurrences in East Asia.

关键词： cold surge Siberian high East Asia East Asian winter monsoon upper-tropospheric circulation

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