检索结果-南通市图书馆

The Splicing Factor PRP31 Is Involved in Transcriptional Gene Silencing and Stress Response in Arabidopsis

Molecular Plant 2015年第7期8卷 1053-1068页

作者： jin-Lu du Su-Wei Zhang Huan-Wei Huang Tao Cai Lin Li She Chen Xin-Jian He College of Life Sciences Beijing Normal University Beijing 100875 China National Institute of Biological Sciences Beijing 102206 China

Although DNA methylation is known to play an important role in the silencing of transposable elements （TEs） and introduced transgenes, the mechanisms that generate DNA methylation-independent transcrip- tional silencing are poorly understood. Previous studies suggest that RNA-directed DNA methylation （RdDM） is required for the silencing of the RD29A-LUC transgene in the Arabidopsis rosl mutant back- ground with defective DNA demethylase. Loss of function of ARGONAUTE 4 （AGO4） gene, which encodes a core RdDM component, partially released the silencing of RD29A-LUC in the rosl/ago4 double mutant plants. A forward genetic screen was performed to identify the mutants with elevated RD29A-LUC trans- gene expression in the rosl/ago4 mutant background. We identified a mutation in the homologous gene of PRP31, which encodes a conserved pre-mRNA splicing factor that regulates the formation of the U4/ U6.U5 snRNP complex in fungi and animals. We previously demonstrated that the splicing factors ZOP1 and STA1 contribute to transcriptional gene silencing. Here, we reveal that Arabidopsis PRP31 associates with ZOP1, STA1, and several other splicing-related proteins, suggesting that these splicing factors are both physically and functionally connected. We show that Arabidopsis PRP31 participates in transcrip- tional gene silencing. Moreover, we report that PRP31, STA1, and ZOP1 are required for development and stress response. Under cold stress, PRP31 is not only necessary for pre-mRNA splicing but also for regulation of cold-responsive gene expression. Our results suggest that the splicing machinery has multiple functions including pre-mRNA splicing, gene regulation, transcriptional gene silencing, and stress response.

关键词： PRP31 splicing factor DNA methylation transcriptional gene silencing stress response

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microneedle-array patch with pH-sensitive formulation for glucoseresponsive insulin delivery

引用

Nano Research 2021年第8期14卷 2689-2696页

作者： Feng-Qin Luo Guojun Chen Wei Xu Daojia Zhou Jia-Xian Li Yong-Cong Huang Run Lin Zhen Gu jin-Zhi du Guangzhou First People’s Hospital and Institutes for Life SciencesSchool of MedicineSouth China University of TechnologyGuangzhou 510006China Department of Bioengineering University of CaliforniaLos AngelesLos AngelesCA 90095-1413USA School of Biomedical Sciences and Engineering Guangzhou International Campus South China University of TechnologyGuangzhou 510006China Department of Radiology the First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Materials and Engineering of the Ministry of EducationKey Laboratory of Biomedical Engineering of Guangdong ProvinceInnovation Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhou 510006China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou 510005China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310058China

Glucose-responsive insulin delivery systems show great promise to improve therapeutic outcomes and quality of life for people with ***,a new microneedle-array patch containing pH-sensitive insulin-loaded nanoparticles(NPs)(SNP(I))together with glucose oxidase(GOx)-and catalase(CAT)-loaded pH-insensitive NPs(iSNP(G+C))is constructed for transcutaneous glucose-responsive insulin ***(I)are prepared via double emulsion from a pH-sensitive amphiphilic block copolymer,and undergo rapid dissociation to promote insulin release at a mild acidic environment induced by GOx in iSNP(G+C)under hyperglycemic *** in iSNP(G+C)can further consume excess H_(2)O_(2) generated during GOx oxidation,and thus reduce the risk of inflammation toward the normal *** in vivo study on type 1 diabetic mice demonstrates that the platform can effectively regulate blood glucose levels within normal ranges for a prolonged period.

关键词： drug delivery diabetes glucose-responsive pH-sensitive microneedle

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pediatric syncope： where are we now？

引用

Chinese Medical Journal 2014年第21期127卷 3681-3683页

作者： jin Hongfang du Junbao Department of Pediatrics Peking University First Hospital Beijing100034 China

Syncope is precisely defined as a temporary loss of consciousness due to global cerebral hypoperfusion,often accompanied with loss of muscular tension and the failure to maintain active position. Pre-syncope is a state consisting of transient lightheadedness, loss of or weaker muscle tension, but not accompanied with loss of consciousness. Syncope and pre-syncope are common symptoms occurring in children and adolescents. Girls present more morbidity than boys. And patients with syncope accounted for about 1%-2% of all pediatric patients in the emergency department.l＇2 Among medical students at an average age of 20 years, one survey found that about 20% of males and 50% of females reported to have syncope at least once.3 The onset of syncope greatly affects the physical and psychological health of children and adolescents, which is a research hotspot in the field of pediatric cardiology at home and abroad.

关键词： syncope child diagnosis management

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pseudo Almost Automorphic Solutions for Non-autonomous Stochastic Differential Equations with Exponential Dichotomy

引用

Communications in Mathematical Research 2014年第2期30卷 139-156页

作者： du jin-shi Sun Kai Wang Yan Li Yong School of Mathematics Jilin University

In this paper, we consider the existence and uniqueness of the solutions which are pseudo almost automorphic in distribution for a class of non-autonomous stochastic differential equations in a Hilbert space. In conclusion, we use the Banach contraction mapping principle and exponential dichotomy property to obtain our main results.

关键词： pseudo almost automorphy exponential dichotomy non-autonomousstochastic differential equation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Crosslinking Kinetics of Polyethylene with Small Amount of Peroxide and Its Influence on the Subsequent Crystallization Behaviors

引用

Chinese Journal of Polymer Science 2016年第9期34卷 1117-1128页

作者：牛艳华 Wen-bin Liang Yin-ling Zhang Xue-lian Chen Shih-yaw Lai Guang-xian Li du-jin Wang College of Polymer Science and Engineering State Key Laboratory of Polymer Materials Engineering of ChinaSichuan UniversityChengdu 610065China Performance Material Center National Institute of Clean-and-low-carbon Energy(NICE)Beijing 102209China CAS Key Laboratory of Engineering Plastics Beijing National Laboratory for Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China

Crosslinking reactions of high density polyethylene with low peroxide concentrations ranging from 0.1 wt% to 1.0 wt% at temperatures of 170, 180 and 190 ℃ were monitored by rheological measurements. A critical gel forms at the peroxide concentration of 0.2 wt%, where the transition from long chain branching generation to crosslinking network formation could occur. Rheokinetics of crosslinking can be fitted well by Ding-Leonov＇s model. The curing rate k2 at the earlier stage exhibits about 3 times acceleration per 10 ~C with increasing temperature, while the equilibrium modulus G＇ at the fully cured stage is almost independent of temperature. Influences of crosslinking on the subsequent crystallization behaviors were detected by DSC measurements. Above the critical gel concentration, crystallization is largely retarded as evidenced by the lower crystallization temperature Tc and crystallinity Xc due to the network formation. The secondary crystallization valley located at the temperature near 80 ℃ can be observed above the critical concentration, which becomes more evident with the increasing peroxide concentration and curing temperature. This phenomenon provides another evidence of crystallization retardation by the crosslinking network.

关键词： Polyethylene Rheology Crosslinking kinetics Crystallization.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prognostic markers for idiopathic pulmonary arterial hypertension

引用

Chinese Medical Journal 2014年第21期127卷 3798-3802页

作者： Guo Xiaomin jin Hongfang du Junbao Department of Pediatrics Peking University First Hospital Beijing 100034 China

Objective The objective of this study is to review the research on the prognostic markers of idiopathic pulmonary arterial hypertension （IPAH）.Date sources We searched literature from PubMed and CNKI databases both in English and Chinese up to *** selection Data about mortality and cut-off value are from clinical trials and identified by *** IPAH is an unexplained,progressive,and rare disease characterized by increased pulmonary artery pressure and pulmonary vascular *** diagnosis is difficult,mortality of IPAH is high,and the survival periods are only 2-3 years after *** in recent years have identified a range of prognostic markers for IPAH,including the 6-minute walking test,red blood cell distribution width,and platelet levels,as well as imaging *** in these markers are important sources of information to predict the prognosis of patients with IPAH,which carries significant benefits for treatment *** Even though the prognosis of IPAH has been investigated,the mortality is also *** accurate and meaningful assessment for the prognosis of IPAH is required.

关键词： pulmonary arterial hypertension prognosis prognostic markers

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis　of　（2E）－α，β－Unsaturated　Amides　Catalyzed　by　Micro　Palladium　Under　Solid－Liquid　Phase－Transfer　Conditions

引用

Chemical Research in Chinese Universities 1995年第1期11卷 71-74页

作者： du jin-xing(Deparrment of Chemistry , Zhejiang Nimal University , jinhua ,321004 )HUANG Xian(Deparrment of Chernistry, Hangzhou Uniuersity , Hangzhou , 310028) ZHEJIANG NORMAL UNIV DEPT CHEMJINHUA 321004PEOPLES R CHINA HANGZHOU UNIV DEPT CHEMHANGZHOU 310028PEOPLES R CHINA

Ｓｙｎｔｈｅｓｉｓｏｆ（２Ｅ）－α，β－ＵｎｓａｔｕｒａｔｅｄＡｍｉｄｅｓＣａｔａｌｙｚｅｄｂｙＭｉｃｒｏＰａｌｌａｄｉｕｍＵｎｄｅｒＳｏｌｉｄ－ＬｉｑｕｉｄＰｈａｓｅ－ＴｒａｎｓｆｅｒＣｏｎｄｉｔｉｏｎｓＤＵＪｉｎ... 详细信息

Ｓｙｎｔｈｅｓｉｓｏｆ（２Ｅ）－α，β－ＵｎｓａｔｕｒａｔｅｄＡｍｉｄｅｓＣａｔａｌｙｚｅｄｂｙＭｉｃｒｏＰａｌｌａｄｉｕｍＵｎｄｅｒＳｏｌｉｄ－ＬｉｑｕｉｄＰｈａｓｅ－ＴｒａｎｓｆｅｒＣｏｎｄｉｔｉｏｎｓＤＵＪｉｎ－ｘｉｎｇ（Ｄｅｐａｒｒｍｅｎｔｏ...

关键词： β-Unsaturated amides Palladium catalyst Phase transfer condi-tions Synthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Special Issue:The Youth Innovation Promotion Association of the Chinese Academy of Sciences

引用

Chinese Journal of Polymer Science 2023年第4期41卷 475-475页

作者： Guo-Ming Liu du-jin Wang 不详

We are pleased to announce the special issue of the Youth Innovation Promotion Association(YIPA)of the Chinese Academy of Sciences(CAS).Established in 2011,YIPA aims to enhance interaction,encourage interdisciplinary collaboration among members,and promote the career development of young researchers,under the age of *** date,YIPA has more than 5000 regular members and more than 600 advanced members,including many researchers actively working in the polymer field.

关键词： Youth encourage collaboration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Sumoylation of SUVR2 contributes to its role in transcriptional gene silencing

引用

Science China(Life Sciences) 2018年第2期61卷 235-243页

作者： Yu-Xi Luo Yong-Feng Han Qiu-Yuan Zhao jin-Lu du Kun Dou Lin Li She Chen Xin-Jian He Graduate School of Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100730 China National Institute of Biological Sciences Beijing 102206 China

The SU(VAR)-3-9-related protein family member SUVR2 has been previously identified to be involved in transcriptional gene silencing both in RNA-dependent and-independent pathways. It interacts with the chromatin-remodeling proteins CHR19,CHR27, and CHR28(CHR19/27/28), which are also involved in transcriptional gene silencing. Here our study demonstrated that SUVR2 is almost fully mono-sumoylated in vivo. We successfully identified the exact SUVR2 sumoylation site by combining in vitro mass spectrometric analysis and in vivo immunoblotting confirmation. The luminescence imaging assay and quantitative RT-PCR results demonstrated that SUVR2 sumoylation is involved in transcriptional gene silencing. Furthermore, we found that SUVR2 sumoylation is required for the interaction of SUVR2 with CHR19/27/28, which is consistent with the fact that SUMO proteins are necessary for transcriptional gene silencing. These results suggest that SUVR2 sumoylation contributes to transcriptional gene silencing by facilitating the interaction of SUVR2 with the chromatin-remodeling proteins CHR19/27/28.

关键词： SUVR2 sumoylation transcriptional gene silencing chromatin-remodeling proteins CHR19

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Chemical Composition and Cooling Process on Structures and Properties of Direct-Quenched Steels

引用

Journal of Iron and Steel Research International 1995年第1期2卷 35-40页

作者： DONG Han LI Gui Fen GAO Wei du jin Kai Central Iron and Steel Research Institute Dalian Steel Works

The microstructures and mechanical properties of a series of direct-quenched steels containing,in weight percent,from 0.05 to 0.20 carbon,0.20 to 0.40 silicon,1.10 to 1.70 manganese and at least one microalloying element of vanadium,niobium,titanium and boron have been *** controlled rolling,these steels were cooled in spray water to 400,300 and 200℃,respectively and then cooled in still *** of the Charpy V-notch impact toughness of direct-quenched steels without subsequent tempering was made with that of direct-quenched steels tempered at 600℃.It is found that an attractive combination of strength and toughness is achived by means of *** exists two types of microstructures in accordance with carbon *** martensitic microstructure is obtained for C>0.4%,granular bainitic microstructures for C.< 0.35%and mixtures of martensite and bainite for C,.in the range of 0.35— 0.40%.The effect of interrupted quenching temperature on microstructure is not significant for low ***,but interrupted quenching temperature has a strong effect on microstructure for high C,.steel.

关键词： direct-quenched steel chemical composition cooling process

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：