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Propagation of Recombinant Genes through Complex Microbiomes with Synthetic Mini-RP4 Plasmid Vectors

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BioDesign Research 2022年第1期2022卷 193-207页

作者： Tomás Aparicio jillian Silbert Sherezade Cepeda Víctor de Lorenzo Systems and Synthetic Biology Department Centro Nacional de Biotecnología(CNB-CSIC)Campus de Cantoblanco Madrid 28049Spain

The promiscuous conjugation machinery of the Gram-negative plasmid RP4 has been reassembled in a minimized, highlytransmissible vector for propagating genetically encoded traits through diverse types of naturally occurring microbialcommunities. To this end, the whole of the RP4-encoded transfer determinants (tra, mob genes, and origin of transfer oriT)was excised from their natural context, minimized, and recreated in the form of a streamlined DNA segment borne by anautoselective replicon. The resulting constructs (the pMATING series) could be self-transferred through a variety ofprokaryotic and eukaryotic recipients employing such a rationally designed conjugal delivery device. Insertion of GFP reporterinto pMATING exposed the value of this genetic tool for delivering heterologous genes to both specific mating partners andcomplex consortia (e.g., plant/soil rhizosphere). The results accredited the effective and functional transfer of the recombinantplasmids to a diversity of hosts. Yet the inspection of factors that limit interspecies DNA transfer in such scenarios uncoveredtype VI secretion systems as one of the factual barriers that check otherwise high conjugal frequencies of tested RP4derivatives. We argue that the hereby presented programming of hyperpromiscuous gene transfer can become a phenomenalasset for the propagation of beneficial traits through various scales of the environmental microbiome.

关键词： otherwise rational transferred

Maternal reproductive health: expression patterns of antioxidant enzyme selenoproteins of post-implantation embryos conceived by ethanol-treated murine mothers supplemented with α-tocopherol

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Asian pacific Journal of Reproduction 2017年第1期6卷 21-25页

作者： Gliceria B Ramos Noel F Alfonso Glenn G Oyong Aleli jillian L Sia Biology Department De La Salle UniversityTaft AvenueManilaPhilippines1004 University Science Foundation-University Research Coordination Office (USF-URCO)De La Salle UniversityManilaPhilippines Molecular Biology Unit CENSERDe La Salle UniversityTaft AvenueManilaPhilippines Department of Biology College of Arts and SciencesUniversity of the Philippines-Manila

Objective:To investigate if the protective effect ofα-tocopherol against the impact of ethanol on brain morphogenesis involved the activity of the selenoproteins phospholipid hydroperoxide glutathione peroxidase (PHGPx;GPx4) and selenoprotein P (SelPP) that have roles against oxidative ***:Forty female mice were randomly assigned into natural control (CON), positive control (eTOH), low-, medium-, and high-α-tocopherol-supplemented-ethanol groups (LTOC, MTOC, HTOC, respectively). CON received drinking water without ethanol while eTOH, LTOC, MTOC and HTOC groups received 20% ethanol in drinking water. The supplemented groups were given respective dosages ofα-tocopherol, 0.410, 0.819, and 1.640 mg/g body weight, at day 14 before mating onwards to the day 9 of gestation. At 10.5 eD of gestation (1100 h), the pregnant females were sacrificed by cervical dislocation and the embryos were harvested. Total RNA were extracted, cDNA synthesis and qRT- PCR analyses were carried ***: The level of expression of PHGPx in the positive control was significantly lower than that of the natural control. Among the threeα-tocopherol-supplemented groups, only the medium dose- group was significantly higher than the positive control. The level of expression of SelPP in the positive control was significantly lower than those of the natural control, the low- and medium- dose -tocopherol supplemented groups. In the high dose-α-tocopherol supplemented group, the level of expression was not significantly different from the positive control but significantly lower than the natural ***: The activity of the selenoproteins PHGPx and SelPP are involved in the internetwork of antioxidative enzymes with vitamin e when given up to a medium dose only and is one of the possible pathways of shielding embryonic development against the impact of ethanol on brain morphogenesis. This study strengthens the impact of dietaryα-tocopherol and Selenium supplement during the criti

关键词： α-tocopherol Dysmorphogenesis ethanol PHGPx Postimplantation embryos Selenoproteins SelPP

ACTIVATION OF NATURAL KILLeR CeLLS WITH 3,6-BIS (2-PIPeRIDINOeTHOXY) ACRIDINe TRIHYDROCHLORIDe (CL 246, 738)

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中华医学杂志(英文版) 1987年第3期100卷 192-197页

作者： Bosco Shang Wang Araceli L. Lumanglas jillian Silva Veronica M. Ruszala-Mallon Frederick e. Durr Laboratory of Tumor Immunology Department of Chemotherapy Research Medical Research Division American Cyanamid Company Lederle Laboratories Pearl River New York 10965

The mechanism of action of 3,6-bis(***-thoxy) acridine trihydrochloride (CL 246,738) in the potentiation of natural killer (NK) cells was investigated. Orally administered CL 246,738 consistently produced enhancement of NK cell activity in mice. Using anti-asialo GM-1 (ASGM-1) antibody as an NK cell mar.er, it was determined that the compound increased the number of ASGM-1 positive cells in mice as indicated by immunofluorescence staining. The compound at concentrations of 0.02-0.2 μg/ml also induced NK cell activity in vitro. This effect was dependent upon the presence of macrophages and was inhibited by anti-IFN (alpha+beta), but not anti-lFN (beta) antibody. Taken together, it is postulated that the compound functions by stimulating macrophages to release IFN (alpha) which subsequently activates NK cells. As an effective stimulator of IFN and NK cells, CL 246,738 may prove clinically useful in the immunotherapy of certain types of malignancy.

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Acupuncture for the Treatment of Hot Flashes in Men with Advanced Prostate Cancer

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International Journal of Clinical Medicine 2011年第1期2卷 51-55页

作者： jillian L. Capodice Philippa Cheetham Mitchell C. Benson James M. McKiernan Aaron e. Katz 不详

PURPOSe: To test the safety and efficacy of acupuncture in reducing hot flashes in men with advanced prostate cancer (CaP) undergoing androgen deprivation therapy. MeTHODS: A single-arm, pilot study was undertaken to evaluate acupuncture treatment for hot flashes experienced by CaP patients undergoing hormonal therapy. Sixteen advanced CaP patients undergoing androgen deprivation therapy (ADT) received standardized full body and auricular acupunc-ture 1x/week for 14 weeks. Patients were evaluated at 0, 7, 14, and an additional 14-wk follow-up (F/U) (28-wks). Safety was measured by monitoring for adverse events over the treatment period. Serum testosterone was measured at 0 and 14-wks. Quality of life (QOL) was measured by the hormone domain of the expanded Prostate Index Composite (ePIC), at the 0, 7, 14, and 28-wks and patient reported hot flash frequency was assessed weekly. Safety endpoints were serum testosterone and analysis of adverse events. efficacy endpoints were scores on the ePIC and the number of pa-tient reported hot flashes. ReSULTS: 17 patients were enrolled and 15 completed the trial. Of 15 evaluable patients the median age was 68 ± 8.19. Serum analysis demonstrated no change in testosterone at baseline 9.5 ng/ml ± 8.9 vs. end-point 14 ± 8.78 (p = 0.101). No adverse events were reported. Data analysis of the ePIC demonstrated a trend toward improvement after 7 wks and a significant improvement following 14 wks of acupuncture (p = 0.01). Analysis of patient reported frequency showed a significant reduction in the number of hot flashes at 7-wk (6, p = 0.04), 14-wk (2.6, p = 0.001), and 28-wk F/U (3.2, p = 0.01) as compared to baseline 9.57 ± 3.98. CONCLUSIONS: The administration of acupuncture in men with advanced CaP appears to significantly decrease the frequency of hot flashes. No serious ad-verse events were noted and serum testosterone levels were unchanged from baseline suggesting that the mechanism of action of acupuncture for hot flash ameliora

关键词： Prostate Cancer Acupuncture Hot Flashes

Sieving characteristics of cytokine-and peroxide-induced epithelial barrier leak:Inhibition by berberine

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World Journal of Gastrointestinal Pathophysiology 2016年第2期7卷 223-234页

作者： Katherine M DiGuilio Christina M Mercogliano jillian Born Brendan Ferraro Julie To Brittany Mixson Allison Smith mary Carmen Valenzano James M Mullin Lankenau Institute for Medical Research Lankenau Medical Center Departments of Biology and Biomedical engineering Drexel University

AIM: To study whether the inflammatory bowel disease (IBD) colon which exhibits varying severity and cytokine levels across its mucosa create varying types of transepithelial leak. MeTHODS: We examined the effects of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1-β (IL1β) and hydrogen peroxide (H2O2) - singly and in combinations - on barrier function of CACO-2 cell layers. Our focus was on the type (not simply the magnitude) of transepithelial leak generated by these agents as measured by transepithelial electrical resistance (TeR) and transepithelial flux of 14C-D-mannitol, 3H-Lactulose and 14C-Polyethylene glycol as radiolabeled probe molecules. The isoquinoline alkaloid, berberine, was then examined for its ability to reduce specific types of transepithelial leak. ReSULTS: exposure to TNF-α alone (200 ng/mL; 48 h) induced a 50% decrease in TeR, i.e., increased leak of Na+ and Cl- - with only a marginal but statistically significant increase in transepithelial leak of 14C-mannitol (Jm). exposure to TNF-α + IFN-γ (200 ng/mL; 48 h) + IL1β (50 ng/mL; 48 h) did not increase the TeR change (from TNF-α alone), but there was now a 100% increase in Jm. There however was no increase in transepithelial leak of two larger probe molecules, 3H-lactulose and 14C-polyethylene glycol (PeG). However, exposure to TNF-α + IFN-γ + IL1β followed by a 5 h exposure to 2 mmol/L H2O2 resulted in a 500% increase in 14C-PeG leak as well as leak to the luminal mitogen, epidermal growth factor. CONCLUSION: This model of graded transepithelial leak is useful in evaluating therapeutic agents reducing IBD morbidity by reducing barrier leak to various luminal substances.

关键词： Intestine Crohn’s disease Tight junction Ulcerative colitis CACO-2 Berberine Micronutrient Cytokine

所有考古学者请注意:稳定同位素在考古学中的应用研究和综述研究中相关术语、方法论、数据处理和报告的指导意见

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东方考古 2022年第1期 212-232页

作者： Patrick Roberts Ricardo Fernandes Oliver e.Craig Thomas Larsen Alexandre Lucquin jillian Swift Jana Zech 毕晓光(译) 董豫(校) 德国马克斯-普朗克人类历史研究所英国剑桥大学麦克唐纳考古研究所英国约克大学考古系德国基督教-阿尔布雷希茨大学莱布尼茨同位素研究实验室山东大学文化遗产研究院

虽然稳定同位素分析方法自20世纪70年代就已应用到考古学中,但仍然缺乏对术语、取样、预处理评价、校正、质量控制、数据呈现,以及图形、统计学处理等方面的标准化方案。本文中,我们针对考古学应用中以下方面逐一提出建议和要求,包括有... 详细信息

虽然稳定同位素分析方法自20世纪70年代就已应用到考古学中,但仍然缺乏对术语、取样、预处理评价、校正、质量控制、数据呈现,以及图形、统计学处理等方面的标准化方案。本文中,我们针对考古学应用中以下方面逐一提出建议和要求,包括有机物总碳、氮稳定同位素分析,碳酸盐总碳、氧稳定同位素分析,胶原蛋白和角质蛋白中单体化合物稳定碳、氮同位素分析,脂肪酸中单体化合物碳、氢稳定同位素分析等。这些规范依据国际纯粹与应用化学联合会(IUPAC)同位素丰度和原子量委员会的建议,以及不断增长的地球化学和科技考古实验文献而制定。考古学领域稳定同位素应用和数据不断增加,我们希望这些规范为相关作者和审稿人提供有价值的参考。

关键词：稳定同位素分析校正分析不确定度统计检验

发育过程中的环境烟草烟雾暴露改变行为并扰乱线粒体能量代谢

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环境与职业医学 2013年第3期30卷 166-166页

作者： Brian F.Fuller Diego F.Cortes Miranda K.Landis Hiyab Yohannes Hailey e.Griffin jillian e.Stafflinger M.Scott Bowers mark H.Lewis Michael A.Fox Andrew K.Ottens

[背景]环境烟草烟雾(eTS)暴露与发育缺陷和疾病相关,已知包括影响小脑的功能。然而,直接的生物学效应及内在的神经化学机制仍不清楚。[目的]明确并评估在大鼠小脑发育的关键时期,eTS暴露所导致的潜在神经化学变化。[方法]将出生后第8天(... 详细信息

[背景]环境烟草烟雾(eTS)暴露与发育缺陷和疾病相关,已知包括影响小脑的功能。然而,直接的生物学效应及内在的神经化学机制仍不清楚。[目的]明确并评估在大鼠小脑发育的关键时期,eTS暴露所导致的潜在神经化学变化。[方法]将出生后第8天(PD8)到第PD23的大鼠,每天暴露于eTS(总悬浮颗粒浓度为300、100和0μg/m3),然后测定行为、神经蛋白质组和细胞水平的反应。[结果]产后eTS暴露诱导在新环境中的运动反应升高,等同于安非他明刺激诱发的效果。eTS暴露大鼠小脑的线粒体亚蛋白质组明显受到干扰。研究结果显示,呈剂量依赖性的需氧过程上调是通过修饰和增加HK1转运至线粒体,伴随ATP合成酶表达的相应提高。eTS暴露也诱导总Dnm1l线粒体分裂因素呈剂量依赖性增加;虽然发现更活跃的膜结合Dnm1l呈低剂量。Dnm1l激活与线粒体染色更强相关,特别是在分子层,它独立于应力诱导的Bcl-2家族的动态。此外,电子显微镜观察显示,Dnm1l介导的线粒体分裂与生物合成增加而非分裂相关。[结论]出生后小脑发育的关键时期对eTS暴露的影响易感,会导致行为改变。eTS的生物效应部分是因为在通常严格控制的情况下,Dnm1l介导的线粒体活跃应答。这些结果显示环境暴露影响神经系统发育和功能的一种新的机制。

关键词：环境烟草烟雾线粒体能量代谢烟雾暴露发育过程神经化学变化行为小脑发育生物学效应