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A LexA-based yeast two-hybrid system for studying light- switchable interactions of phytochromes with their interacting partners

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aBIOTECH 2021年第2期2卷 105-116页

作者： Hong Li Xinyan Qin Pengyu Song Run han jigang Li State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Phytochromes are a family of photoreceptors in plants that perceive the red(R)and far-red(FR)components of their light *** exist in vivo in two forms,the inactive Pr form and the active Pfr form,that are interconvertible by treatments with R or FR *** is believed that phytochromes transduce light signals by interacting with their signaling partners.A GAL4-based lightswitchable yeast two-hybrid(Y2H)system was developed two decades ago and has been successfully employed in many studies to determine phytochrome interactions with their signaling ***,several pairs of interactions between phytochromes and their interactors,such as the phyACOP1 and phyA-TZP interactions,were demonstrated by other assay systems but were not detected by this GAL4 Y2H ***,we report a modified LexA Y2H system,in which the LexA DNA-binding domain is fused to the C-terminus of a phytochrome *** conformational changes of phytochromes in response to R and FR light are achieved in yeast cells by exogenously supplying phycocyanobilin(PCB)extracted from *** well-defined interaction pairs,including phyA-FHY1 and phyB-PIFs,are well reproducible in this ***,we show that our system is successful in detecting the phyA-COP1 and phyA-TZP ***,our study provides an alternative Y2H system that is highly sensitive and reproducible for detecting light-switchable interactions of phytochromes with their interacting partners.

关键词： Phytochrome Interaction Yeast two-hybrid LexA Light-switchable

Twinning Behaviour in the Intermetallic Compound Al_(18)Cr_2Mg_3

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Acta Metallurgica Sinica(English Letters) 2014年第4期27卷 677-681页

作者： Maohua Li Yanqing Yang Ming han Wei Zhang Bin Huang Xian Luo jigang Ru State Key Laboratory of Solidification Processing Northwestern Polytechnical University Beijing Institute of Aeronautical Materials

The twinning in the intermetallic compound Al18Cr2Mg3 was observed and identified by transmission electron *** geometric description of the twinning in Al18Cr2Mg3 with 184 atoms per unit cell was investigated at atomic *** classical theory of deformation twinning states that the net atomic displacements are divided into shear and shuffles during *** on the theory and the hypothesis of full mirror reflection symmetry,the displacement and angle of shuffle were calculated for each *** geometric calculation results show that all of the atoms in single crystal Al18Cr2Mg3 have to ***,the calculation results can be divided into 22 groups,which do not show obvious regularity.

关键词： Intermetallic compound Al18Cr2Mg3 Twinning Shear Shuffle

Impairment of the autophagy-lysosomal pathway in Alzheimer’s diseases: Pathogenic mechanisms and therapeutic potential

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Acta Pharmaceutica Sinica B 2022年第3期12卷 1019-1040页

作者： Wei Zhang Chengchao Xu Jichao Sun han-Ming Shen jigang Wang Chuanbin Yang Department of Geriatrics Shenzhen People’s Hospital(the Second Clinical Medical CollegeJinan Universitythe First Affiliated HospitalSouthern University of Science and Technology)Shenzhen 518020China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Artemisinin Research Center Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Faculty of Health Sciences University of MacaoTaipaMacao 999078China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China

Alzheimer’s disease(AD),the most common neurodegenerative disorder,is characterized by memory loss and cognitive *** accumulation of misfolded protein aggregates including amyloid beta(Aβ)peptides and microtubule associated protein tau(MAPT/tau)in neuronal cells are hallmarks of *** far,the exact underlying mechanisms for the aetiologies of AD have not been fully understood and the effective treatment for AD is *** is an evolutionarily conserved cellular catabolic process by which damaged cellular organelles and protein aggregates are degraded via ***,there is accumulating evidence linking the impairment of the autophagy-lysosomal pathway with AD ***,the enhancement of autophagy to remove protein aggregates has been proposed as a promising therapeutic strategy for ***,we first summarize the recent genetic,pathological and experimental studies regarding the impairment of the autophagy-lysosomal pathway in *** then describe the interplay between the autophagy-lysosomal pathway and two pathological proteins,Aβand MAPT/tau,in ***,we discuss potential therapeutic strategies and small molecules that target the autophagy-lysosomal pathway for AD treatment both in animal models and in clinical ***,this article highlights the pivotal functions of the autophagy-lysosomal pathway in AD pathogenesis and potential druggable targets in the autophagy-lysosomal pathway for AD treatment.

关键词： Alzheimer’s disease(AD) Amyloid beta(Aβ)peptides MAPT/tau Autophagy-lysosomal pathway Autophagy enhancers Autophagy Mitophagy Neurodegenerative diseases

难降解生活源废物填料生物反应器预处理老龄垃圾渗滤液

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山东化工 2021年第1期50卷 245-247,249页

作者：韩继刚张建旻徐跃鹰吴俊哲袁彭胜赵由才周涛李天上海城投环境(集团)有限公司上善分公司上海201799 同济大学环境科学与工程学院上海200092

渗滤液具有成分复杂、含盐量高、毒性大等特点,如果直接排放会对周围环境造成严重危害,生物处理因其成本低,是目前主要处理技术。基于生物反应床工艺,将生活垃圾转运废弃集装箱改制为生物反应床体,以废旧织物、动物骨料、泡沫混凝土、... 详细信息

渗滤液具有成分复杂、含盐量高、毒性大等特点,如果直接排放会对周围环境造成严重危害,生物处理因其成本低,是目前主要处理技术。基于生物反应床工艺,将生活垃圾转运废弃集装箱改制为生物反应床体,以废旧织物、动物骨料、泡沫混凝土、矿化垃圾等难降解生活源废物为填料,用于预处理渗滤液,当进水量为1400 L/d时,对老龄渗滤液COD去除率在40%以上,总氮去除率稳定在80%左右。该装置系统简单、成本低且无需复杂维护,同时对调节池总氮具有显著的去除效果,应用前景良好。

关键词：老龄垃圾渗滤液生物反应床难降解生物源废物填料脱氮

Task Graph Reduction Algorithm for Hardware/Software Partitioning

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Wuhan University Journal of Natural Sciences 2012年第2期17卷 126-130页

作者： LI Hui LIU Wenjui WU jigang JIANG Guiyuan han Honglei School of Computer Science and Software Tianjin Polytechnic University Tianjin 300387 China State Key Laboratory of Computer Science Institute of Software Chinese Academy of Sciences Beijing 100190 China

Hardware/software（HW/SW） partitioning is one of the key processes in an embedded *** is used to determine which system components are assigned to hardware and which are processed by *** contrast with previous research that focuses on developing efficient heuristic,we focus on the pre-process of the task graph before the HW/SW partitioning in this paper,that is,enumerating all the sub-graphs that meet the *** results showed that the original graph can be reduced to 67% in the worst-case scenario and 58% in the best-case *** conclusion,the reduced task graph saved hardware area while improving partitioning speed and accuracy.

关键词： HW/SW partitioning task graph algorithm embedded system

Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2300-2314页

作者： Piao Luo Dandan Liu Qian Zhang Fan Yang Yin-Kwan Wong Fei Xia Junzhe Zhang Jiayun Chen Ya Tian Chuanbin Yang Lingyun Dai han-Ming Shen jigang Wang Artemisinin Research Center Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Central People’s Hospital of Zhanjiang Zhanjiang 524037China Department of Urology the Second Clinical Medical CollegeJinan University(Shenzhen People’s Hospital)Shenzhen 518020China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Faculty of Health Sciences University of MacaoTaipaMacao 999078China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China

Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron-and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species(ROS) and inducing ferroptosis in activated hepatic stellate cells(HSCs). By using activity-based protein profiling(ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay(CETSA), we show that celastrol directly binds to peroxiredoxins(PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6,through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1(HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2,PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis.

关键词： Celastrol Ferroptosis Peroxiredoxin HO-1 Hepatic fibrosis ABPP Anti-oxidant Reactive oxygen species

Traditional Chinese medicine in COVID-19

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Acta Pharmaceutica Sinica B 2021年第11期11卷 3337-3363页

作者： Ming Lyu Guanwei Fan Guangxu Xiao Taiyi Wang Dong Xu Jie Gao Shaoqin Ge Qingling Li Yuling Ma han Zhang jigang Wang Yuanlu Cui Junhua Zhang Yan Zhu Boli Zhang State Key Laboratory of Component-based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Artemisinin Research Center Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion First Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300193China Oxford Chinese Medicine Research Centre University of OxfordOxford OX13PTUK College of Traditional Chinese Medicine Hebei UniversityBaoding 071002China Institute of Basic Medicine and Cancer the Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)Chinese Academy of SciencesHangzhou 310022China

COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2(SARSCo V-2)has spread across the globe,posing an enormous threat to public health and *** Chinese medicine(TCM),in combination with Western medicine(WM),has made important and lasting contributions in the battle against *** this review,updated clinical effects and potential mechanisms of TCM,presented in newly recognized three distinct phases of the disease,are summarized and *** integrating the available clinical and preclinical evidence,the efficacies and underlying mechanisms of TCM on COVID-19,including the highly recommended three Chinese patent medicines and three Chinese medicine formulas,are described in a *** hope that this comprehensive review not only provides a reference for health care professionals and the public to recognize the significant contributions of TCM for COVID-19,but also serves as an evidence-based in-depth summary and analysis to facilitate understanding the true scientific value of TCM.

关键词： COVID-19 SARS-CoV-2 Traditional Chinese medicine Clinical evidence Potential mechanism Viral infection Cytokine storm Multiple organ dysfunction

Synergistic effects of autophagy/mitophagy inhibitors and magnolol promote apoptosis and antitumor efficacy

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Acta Pharmaceutica Sinica B 2021年第12期11卷 3966-3982页

作者： Yancheng Tang Liming Wang Tao Yi Jun Xu jigang Wang Jiang-Jiang Qin Qilei Chen Ka-Man Yip Yihang Pan Peng Hong Yingying Lu han-Ming Shen Hu-Biao Chen School of Chinese Medicine Hong Kong Baptist UniversityHong Kong SAR 999077China School of Biomedical Sciences Hunan UniversityChangsha 410082China Department of Physiology Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Artemisinin Research Center China Academy of Chinese Medical SciencesBeijing 100700China The First Affiliated Hospital of Southern University of Science and Technology the Second Clinical Medical College of Jinan UniversityShenzhen People’s HospitalShenzhen 518020China The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou 310022China epartment of Medical Research Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China Department of Biomedical Science City University of Hong KongHong Kong SAR 999077China Faculty of Health Sciences University of MacaoMacao SAR 999078China

Mitochondria as a signaling platform play crucial roles in deciding cell *** classic anticancer agents are known to trigger cell death through induction of mitochondrial ***,one selective autophagy,is the key mitochondrial quality control that effectively removes damaged ***,the precise roles of mitophagy in tumorigenesis and anticancer agent treatment remain largely ***,we examined the functional implication of mitophagy in the anticancer properties of magnolol,a natural product isolated from herbal Magnolia ***,we found that magnolol induces mitochondrial depolarization,causes excessive mitochondrial fragmentation,and increases mitochondrial reactive oxygen species(mtROS).Second,magnolol induces PTEN-induced putative kinase protein 1(PINK1)-Parkin-mediated mitophagy through regulating two positive feedforward amplification ***,magnolol triggers cancer cell death and inhibits neuroblastoma tumor growth via the intrinsic apoptosis ***,magnolol prolongs the survival time of tumor-bearing ***,inhibition of mitophagy by PINK1/Parkin knockdown or using inhibitors targeting different autophagy/mitophagy stages significantly promotes magnolol-induced cell death and enhances magnolol's anticancer efficacy,both in vitro and in ***,our study demonstrates that magnolol can induce autophagy/mitophagy and apoptosis,whereas blockage of autophagy/mitophagy remarkably enhances the anticancer efficacy of magnolol,suggesting that targeting mitophagy may be a promising strategy to overcome chemoresistance and improve anticancer therapy.

关键词： PINK1-Parkin-mediated mitophagy Magnolol Combination therapy Apoptosis Tumor suppression

Distributed Malware Detection based on Binary File Features in Cloud Computing Environment

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Distributed Malware Detection based on Binary File Features ...