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Nano Research 2024年第3期17卷 1281-1287页

作者： jiejie ping Danyang He Fei Wang Nan Wang Yi-cheng Fu Zihao Xing Zhiyu Jia Guo-Yu Yang Key Laboratory of Cluster Science Ministry of EducationSchool of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081China Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education Facluty of Chmeistry Northeast Normal UniversityChangchun 130024China Peking University Third Hospital Beijing 100190China

Polyoxometalate-based nanocomposites with electrocatalytic activity have been applied in hydrogen evolution reactions(HER).Seawater as the main water resource on the earth should be developed as the water electrolysis to prepare high-purity hydrogen.In this paper,we used two synthesis strategies to prepare the nanocomposite Co_(4)-POM@Co-PGDY(Co_(4)-POM:the Kegging-type microcrystals of K_(10)[Co_(4)(PW_(9)O_(3)4)2]and Co-PGDY:cobalt-porphyrin linked graphdiyne)with excellent activity for HER.Co-PGDY as the porous material is applied not only as the protection of microcrystals towards the metal ion in seawater but also as the co-electrocatalyst of Co_(4)-POM.Co_(4)-POM@Co-PGDY exhibits excellent HER performance in seawater electrolytes with low overpotential and high stability at high density.Moreover,we have observed a key H_(3)O+intermediate emergence on the surface of nanocomposite during hydrogen evolution process in seawater by Raman synchrotron radiation-based Fourier transform infrared(SR-FTIR).The results in this paper provide an effective strategy for preparing polyoxometalate-based electrocatalysts with high-performance toward hydrogen evolution reaction.

关键词： polyoxometalate graphdiyne seawater hydrogen evolution reaction

数字技能如何影响农民电商参与行为?--基于信息获取的中介效应分析

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中国农业大学学报 2024年第4期29卷 81-93页

作者：蒋洁洁青平蔡炜炜黄帆华中农业大学经济管理学院武汉430070

为探究数字技能对农民电商参与行为的影响及作用机制,基于2020年中国乡村振兴综合调查(CRRS)数据,立足“手段-自由-成就”的可行信息能力理论,构建“数字技能-信息获取-电商参与”的理论分析框架,采用二元Probit、Tobit和中介效应等分... 详细信息

为探究数字技能对农民电商参与行为的影响及作用机制,基于2020年中国乡村振兴综合调查(CRRS)数据,立足“手段-自由-成就”的可行信息能力理论,构建“数字技能-信息获取-电商参与”的理论分析框架,采用二元Probit、Tobit和中介效应等分析方法,从信息获取的中介视角实证检验数字技能对农民电商参与决策及程度的影响,并进行稳健性检验和异质性分析。结果表明:1)数字技能对农民电商参与决策和程度均具有显著正向影响;2)信息获取在数字技能对农民电商参与决策和程度的影响中存在部分中介效应,中介效应占总效应的比重分别为11.76%和11.73%;3)数字技能对收入水平较高、受教育水平较高的农民,电商参与决策和参与程度的正向影响更为显著。政府应从有效提升农民数字技能水平,稳步实现信息获取自由,加强关注农民内部分化3方面提升农民电商参与率和程度。

关键词：数字技能电商参与决策电商参与程度信息获取

雌二醇对6-羟基多巴胺诱导PD模型SIRT1表达的影响

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脑与神经疾病杂志 2024年第4期32卷 203-208页

作者：温雅张婕婕张海涛杨一涵李萍王惠娟河北医科大学第二医院神经内科石家庄050000

目的探讨雌二醇(E2)对6-羟基多巴胺(6-OHDA)诱导的PC12细胞帕金森病(PD)模型中沉默信息调节因子1(SIRT1)表达及PD大鼠外周血及脑组织SIRT1的影响。方法(1)将6-OHDA作用的嗜络细胞瘤细胞(PC12),给予不同浓度的E2进行干预,根据E2干预浓度... 详细信息

目的探讨雌二醇(E2)对6-羟基多巴胺(6-OHDA)诱导的PC12细胞帕金森病(PD)模型中沉默信息调节因子1(SIRT1)表达及PD大鼠外周血及脑组织SIRT1的影响。方法(1)将6-OHDA作用的嗜络细胞瘤细胞(PC12),给予不同浓度的E2进行干预,根据E2干预浓度的的不同进行分组:PD模型组、E2低剂量组(E21×10^(-8)mol·L^(-1))、E2中剂量组(E21×10^(-7)mol·L^(-1))、E2高剂量(E21×10^(-6)mol·L^(-1)),正常细胞作为对照组。采用四甲基偶氮唑蓝(MTT)法检测各组细胞存活率,流式细胞仪检测凋亡率,免疫细胞化学法检测络氨酸羟化酶(TH)和SIRT1表达;(2)采用6-OHDA诱导并制备PD大鼠模型,将PD大鼠分为PD模型组、E2低剂量组(25μg·kg^(-1))、E2中剂量组(50μg·kg^(-1))和E2高剂量组(100μg·kg^(-1)),将健康大鼠作为对照组,10只/组。对照组和PD模型组灌胃给予蒸馏水,E2处理组分别灌胃给予E2。采用ELISA法检测外周血及脑组织的TH及SIRT1蛋白表达。结果(1)MTT法显示,与对照组比较,模型组的凋亡率(AR)和细胞生长抑制率(CGIR)显著增高,与模型组比较,E2低、中和高剂量组AR和CGIR明显降低,且呈E2浓度依赖性趋势。免疫细胞化学染色显示,TH和SIRT1蛋白在PC12细胞呈现黄色或者棕黄色染色,与对照组比较,模型组的TH和SIRT1显著降低;与模型组比较,E2低、中和高剂量组TH和SIRT1明显升高,且呈E2浓度依赖性趋势;(2)与对照组比较,PD模型组的TH和SIRT1显著降低;与PD模型组比较,E2低、中和高剂量组TH和SIRT1明显升高,且呈E2浓度依赖性趋势。结论E2可能通过激活SIRT1抑制6-OHDA诱导的PD模型而发挥治疗PD的作用,该作用呈现E2浓度依赖性关系。

关键词：帕金森病雌二醇沉默信息调节因子1 大鼠嗜铬细胞瘤细胞

Serine 707 of APPL1 is Critical for the Synaptic NMDA Receptor-Mediated Akt Phosphorylation Signaling Pathway

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Neuroscience Bulletin 2016年第4期32卷 323-330页

作者： jiejie Wang Wen Lu Lin Chen ping Zhang Tingting Qian Wei Cao Jianhong Luo Department of Neurobiology Zhejiang University School of Medicine The Second Affiliated Hospital Zhejiang University School of Medicine Sir Run Run Shaw Hospital Zhejiang University School of Medicine

Accumulating evidence indicates that the synaptic activation of N-methyl-o-aspartate receptors （NMDARs） has a neuroprotective effect on neurons. Our previous study demonstrated that APPL1 （adaptor protein containing pleckstrin homology domain, phosphotyrosine- binding domain, and leucine zipper motif） mediates the synaptic activity-dependent activation of PI3K-Akt signaling via coupling this pathway with NMDAR-PSD95 （postsynaptic density protein 95） complexes. However, the molecular mechanism underlying this process is still unknown. In the present study, we investigated the inter- action of APPL1 with PSD95 using co-immunocyto- chemical staining and western blotting. We found that the PDZ2 domain of PSD95 is a binding partner of APPL1. Furthermore, we identified serine 707 of APPL1, a pre- dicted phosphorylation site within the PDZ-binding motif at the C-terminus, as critical for the binding of APPL1 to PSD95, as well as for activation of the Akt signaling pathway during synaptic activity. This suggests that serine 707 of APPL1 is a potential phosphorylation site and may be involved in regulating the neuroprotective Akt signaling pathway that depends on synaptic NMDAR activity.

关键词： APPL1 PSD95 Akt NMDA receptors Neuroprotection

Immunological and metabolic characteristics of the Omicron variants infection

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Signal Transduction and Targeted Therapy 2023年第2期8卷 784-794页

作者： jiejie Geng Xu Yang Kun Wang Ke Wang Ruo Chen Zhi-Nan Chen Chuan Qin Guizhen Wu Youchun Wang Ke Xu Peng Du Jiangning Liu Shirui Chen Tao Zhang Xiuxuan Sun Ting Guo Ying Shi Zheng Zhang Ding Wei Peng Lin Qingyi Wang Jing Yuan Jiuxin Qu Jin Zou Yingxia Liu Hongzhou Lu ping Zhu Huijie Bian Liang Chen Department of Cell Biology of National Translational Science Center for Molecular Medicine and Department of Clinical Immunology of Xijing Hospital Fourth Military Medical UniversityXi’an 710032China Key Laboratory of Human Disease Comparative Medicine Chinese Ministry of HealthKey Laboratory for Animal Models of Emerging and Remerging Infectious DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100871China MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Preven-tionChinese Center for Disease Control and PreventionBeijing 100871China Division of HIV/AIDS and Sex-transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)and WHO Collaborating Center for Standardization and Evaluation of BiologicalsBeijing 102629China Beijing Institute of Biotechnology Beijing 100871China School of Basic Medicine Fourth Military Medical UniversityXi’an 710032China The Third People’s Hospital of Shenzhen Shenzhen 518112China School of Medicine Shanghai UniversityShanghai 200444China

The Omicron variants of SARS-CoV-2,primarily authenticated in November 2021 in South Africa,has initiated the 5th wave of global pandemics.Here,we systemically examined immunological and metabolic characteristics of Omicron variants infection.We found Omicron resisted to neutralizing antibody targeting receptor binding domain(RBD)of wildtype SARS-CoV-2.Omicron could hardly be neutralized by sera of Corona Virus Disease 2019(COVID-19)convalescents infected with the Delta variant.Through mass spectrometry on MHC-bound peptidomes,we found that the spike protein of the Omicron variants could generate additional CD8+T cell epitopes,compared with Delta.These epitopes could induce robust CD8+T cell responses.Moreover,we found booster vaccination increased the cross-memory CD8+T cell responses against Omicron.Metabolic regulome analysis of Omicron-specific T cell showed a metabolic profile that promoted the response of memory T cells.Consistently,a greater fraction of memory CD8+T cells existed in Omicron stimulated peripheral blood mononuclear cells(PBMCs).In addition,CD147 was also a receptor for the Omicron variants,and CD147 antibody inhibited infection of Omicron.CD147-mediated Omicron infection in a human CD147 transgenic mouse model induced exudative alveolar pneumonia.Taken together,our data suggested that vaccination booster and receptor blocking antibody are two effective strategies against Omicron.

关键词： infection inhibited neutral

CD98-induced CD147 signaling stabilizes the Foxp3 protein to maintain tissue homeostasis

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Cellular & Molecular Immunology 2021年第12期18卷 2618-2631页

作者： jiejie Geng Ruo Chen Feng-fan Yang Peng Lin Yu-meng Zhu Xianghui Fu Ke Wang Zhuan Feng Jiao Wu Hai Zhang Qi-jing Li Zhi-Nan Chen ping Zhu Department of Clinical Immunology Xijing HospitalFourth Military Medical UniversityXi’anP.R.China National Translational Science Center for Molecular Medicine&Department of Cell Biology Fourth Military Medical UniversityXi’anP.R.China Department of Immunology Duke University Medical CenterDurhamNCUSA

Regulatory T cell(Treg)stability is necessary for the proper control of immune activity and tissue homeostasis.However,it remains unclear whether Treg stability must be continually reinforced or is established during development under physiological conditions.Foxp3 has been characterized as a central mediator of the genetic program that governs Treg stability.Here,we demonstrate that to maintain Foxp3 protein expression,Tregs require cell-to-cell contact,which is mediated by the CD147-CD98 interaction.As Tregs are produced,CD147,which is expressed on their surface,is stimulated by CD98,which is widely expressed in the physiological environment.As a result,CD147’s intracellular domain binds to CDK2 and retains it near the membrane,leading to Foxp3 dephosphorylation and the prevention of Foxp3 degradation.In addition,the optimal distribution of Foxp3+Tregs under both pathological and physiological conditions depends on CD98 expression.Thus,our study provides direct evidence that Foxp3-dependent Treg stability is reinforced in the periphery by the interaction between CD147 and CD98 in the surrounding environment.More importantly,Tregs with high CD147 expression effectively inhibit inflammatory responses and maintain Foxp3 stability,which has guiding significance for the application of Tregs in immunotherapy.

关键词： Treg Stability CD147 CD98 CDK2 Immune therapy

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嘉兴老建委驿站,嘉兴,浙江,中国

世界建筑 2024年第6期 78-83页

作者：无陈夏未柯礼钧沈俊彦王凯金拓汪剑锋李磊黄志勇王杰杰王小军墨亚涛蒋金晖方晓灵胡涛邱平李成虎中国美术学院风景建筑设计研究总院青创中心不详奥观建筑视觉

老建委驿站位于嘉兴市老城核心中山路,场地夹在不同年代的大楼缝隙中,属于典型的城市碎片空间。项目是嘉兴老城重塑计划中的一部分,区别于大规模的整体更新,在碎片空间中引入简·雅各布斯的“街道眼”概念,以“器官化”的点式更新来唤... 详细信息

老建委驿站位于嘉兴市老城核心中山路,场地夹在不同年代的大楼缝隙中,属于典型的城市碎片空间。项目是嘉兴老城重塑计划中的一部分,区别于大规模的整体更新,在碎片空间中引入简·雅各布斯的“街道眼”概念,以“器官化”的点式更新来唤起人们对老城复杂多样生活的热爱。场地最大的特征是无序、边界模糊,以及4棵枝繁叶茂的香樟。项目的目标是重新利用场地,创造活跃的社区活动空间并满足公共卫生间要求。建筑以“大树底下好乘凉”的姿态介入场地的复杂环境,在不规则的场地上建立起3个院子来保留4棵香樟,限定边界,整个屋面都在树荫之下。

关键词：边界模糊公共卫生间嘉兴社区活动驿站中山路香樟

CD147 contributes to SARS-CoV-2-induced pulmonary fibrosis

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Signal Transduction and Targeted Therapy 2022年第12期7卷 4422-4434页

作者： Jiao Wu Liang Chen Chuan Qin Fei Huo Xue Liang Xu Yang Kui Zhang Peng Lin Jiangning Liu Zhuan Feng Jiansheng Zhou Zhuo Pei Yatao Wang Xiu-Xuan Sun Ke Wang jiejie Geng Zhaohui Zheng Xianghui Fu Man Liu Qingyi Wang Zheng Zhang Huijie Bian ping Zhu Zhi-Nan Chen Department of Cell Biology of National Translational Science Center for Molecular Medicine and Department of Clinical Immunology of Xijing Hospital Fourth Military Medical UniversityXi’an710032China Shanghai Engineering Research Center of Organ Repair School of MedicineShanghai UniversityShanghai200444China Institute of Laboratory Animals Science Chinese Academy of Medical SciencesBeijing100071China

COVID‐19 patients can develop clinical and histopathological features associated with fibrosis,but the pathogenesis of fibrosis remains poorly understood.CD147 has been identified as a universal receptor for SARS-CoV-2 and its variants,which could initiate COVID-19-related cytokine storm.Here,we systemically analyzed lung pathogenesis in SARS-CoV-2-and its delta variant-infected humanized CD147 transgenic mice.Histopathology and Transmission Electron Microscopy revealed inflammation,fibroblast expansion and pronounced fibrotic remodeling in SARS-CoV-2-infected lungs.Consistently,RNA-sequencing identified a set of fibrosis signature genes.Furthermore,we identified CD147 as a crucial regulator for fibroblast activation induced by SARS-CoV-2.We found conditional knockout of CD147 in fibroblast suppressed activation of fibroblasts,decreasing susceptibility to bleomycin-induced pulmonary fibrosis.Meplazumab,a CD147 antibody,was able to inhibit the accumulation of activated fibroblasts and the production of ECM proteins,thus alleviating the progression of pulmonary fibrosis caused by SARS-CoV-2.In conclusion,we demonstrated that CD147 contributed to SARS-CoV-2-triggered progressive pulmonary fibrosis and identified CD147 as a potential therapeutic target for treating patients with post-COVID-19 pulmonary fibrosis.

关键词： CD147 inflammation fibrosis

Research of New Cloud Agent Game Model

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Research of New Cloud Agent Game Model

宫颈微小浸润癌宫颈锥切术后子宫病灶残留影响因素分析

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作者： ping Jiang Haixia Li jiejie Chen Computer School Hubei PolyTechnic University College of Computer science and technology Hubei Normal University

In the cloud computing environment,each user expects to achieve higher quality of service.Different quality of service（QoS） requirements for users implies their different preferences.When each user tends to choose the resources with the prime computing power,some resources with strong computing power will be selected by multiple users,resulting in the overload of these resources and the decline of comprehensive performance.At this time,the overall resource allocation of the system is not optimal.Accordingly,a number of users have formed a competitive relationship with some key resources.Aiming at those problems,a cloud agent game model has been designed in this paper.Under the premise of satisfying the user’s QoS multi-objective optimization requirements,all cloud agents in the cloud computing system form a decentralized self organizing cloud agent system.The cloud proxy is created along with the request of the individual task scheduling,and the cloud proxy obtains the optimal resource through the mode of autonomous competition game.Then,revocation occurs as the users’ task scheduling completes,while the whole task scheduling process has dynamic scalability and adaptability,so that the overall system achieves the interest of the whole.

关键词： Cloud computing QoS Agent Game

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浙江医学 2016年第24期38卷 1989-1992页

作者：章杰捷张平浙江省肿瘤医院妇瘤科杭州310022

目的探讨宫颈微小浸润癌(MIC)宫颈锥切术后子宫病灶残留的影响因素。方法收集经宫颈锥切术后病理明确诊断为MIC并在3个月内进一步行子宫切除术的患者149例,将其年龄、绝经情况、临床分期、术前血清鳞状细胞癌抗原(SCC)检测数值、术前阴... 详细信息

目的探讨宫颈微小浸润癌(MIC)宫颈锥切术后子宫病灶残留的影响因素。方法收集经宫颈锥切术后病理明确诊断为MIC并在3个月内进一步行子宫切除术的患者149例,将其年龄、绝经情况、临床分期、术前血清鳞状细胞癌抗原(SCC)检测数值、术前阴道分泌物HPV分型检测结果等与宫颈切缘情况及子宫全切标本的病理结果相结合进行分析,探讨病灶残留的影响因素。结果有无子宫病灶残留的患者在年龄、是否绝经、临床分期、术前SCC水平和浸润情况的比较差异均无统计学意义(均P>0.05),而在宫颈切缘和HPV16/18感染是否阳性的比较差异均有统计学意义(均P0.05)。宫颈切缘阳性与阴性者的HPV16/18阳性率的比较差异有统计学意义(P<0.05)。结论术前高危HPV16/18感染以及宫颈切缘阳性均与子宫病灶残留密切相关,HPV16/18感染为子宫病灶残留的独立危险因素。修改诊断切缘阴性的标准是否能更准确判断病灶残留情况以及是否需要扩大术前高危HPV16/18亚型感染患者的锥切手术范围有待进一步研究。

关键词： MIC 宫颈锥切术宫颈切缘人类乳头瘤病毒病灶残留