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Optimal containment control of continuous-time multi-agent systems with unknown disturbances using data-driven approach

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Science China(Information Sciences) 2020年第10期63卷 270-272页

作者： Zhinan PENG jiefu zhang Jiangping HU Rui HUANG Bijoy Kumar GHOSH School of Automation Engineering University of Electronic Science and Technology of China Department of Mathematics and Statistics Texas Tech University

Dear editor,Containment control problems of multi-agent systems (MASs) have attracted considerable attention in recent years owing to their widespread use in applications pertaining to spacecraft control,sensor networ... 详细信息

关键词： High-order multi-agent systems unmatched disturbances Containment control critic- actor network model transformation

Development and molecular analysis of a novel acetohydroxyacid synthase rapeseed mutant with high resistance to sulfonylurea herbicides

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The Crop Journal 2022年第1期10卷 56-66页

作者： Yue Guo Changle Liu Weihua Long Jianqin Gao jiefu zhang Song Chen Huiming Pu Maolong Hu Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Key Laboratory of Cotton and Rapeseed(Nanjing)Ministry of AgricultureNanjing 210014JiangsuChina Institute of Life Sciences Jiangsu UniversityZhenjiang 212013JiangsuChina School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina

With the increasing promotion of simplified rapeseed cultivation in recent years,the development of cultivars with high resistance to herbicides is urgently *** previously developed M342,which shows sulfonylurea herbicide resistance,by targeting acetohydroxyacid synthase(AHAS),a key enzyme in branched-chain amino acid *** the present study,we used a progeny line derived from M342 for an additional round of ethyl methane sulfonate mutagenesis,yielding the novel mutant DS3,which harbored two mutations in AHAS genes and showed high sulfonylurea *** mutation was the substitution Trp574 Leu,as in M342,according to Arabidopsis protein *** other site was a newly recognized substitution,Pro197 Leu.A KASP marker targeting Pro197 Leu was developed and reliably predicted the response to sulfonylurea herbicides in the F2 *** combination of Trp574 Leu and Pro197 Leu in DS3 produced a synergistic effect that greatly increased herbicide *** of the protein structures of AHAS1 and AHAS3 in wild-type and single-gene mutant plants revealed three-dimensional protein conformational changes that could account for differences in herbicide resistance characteristics including toxicity tolerance,AHAS enzyme activity,and AHAS gene expression.

关键词： Acetohydroxyacid synthase High-resistance rapeseed Additional round of mutagenesis Sulfonylurea herbicide Synergistic mutations

***2 regulates plant height by affecting the main inflorescence length and first effective branch height in Brassica napus L.

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The Crop Journal 2024年第4期12卷 1102-1111页

作者： Chengke Pang Jun Yu Liang zhang Min Tang Hongfang Liu Ying Cai Feng Chen jiefu zhang Wei Hua Xiaodong Wang Ming Zheng Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture and Rural AffairsWuhan 430062HubeiChina Key Laboratory of Cotton and Rapeseed Ministry of Agriculture and Rural AffairsKey Laboratory of Jiangsu Province for AgrobiologyInstitute of Industrial CropsJiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Rapeseed(Brassica napus L.)is one of the main oil crops in the world,and increasing its yield is of great significance for ensuring the safety of edible ***,improving rapeseed plant architecture is an effective way to increase rapeseed yield with higher planting ***,the regulatory mechanism of rapeseed plant architecture is poorly *** this study,a dwarf rapeseed mutant dwarf08(df08)is obtained by ethyl methane sulfonate(EMS)-*** decrease in plant height of df08 is mainly caused by the reduction in main inflorescence length and first effective branch height and controlled by a single semi-dominant *** hybrid plants(F1)show a semi-dwarf *** map-based cloning and transgenic assay,we confirm that the nonsynonymous single nucleotide variant(SNV)(C to T)in ***2,which is homologous with Arabidopsis(Arabidopsis thaliana)BIN2,is responsible for the dwarfism of ***2 interacts with BnaBZR1/BES1 and involves in brassinosteroids(BRs)signal *** to Leucine substitution in 284(P284L)enhances the protein stability of ***2-D,disrupts BRs signal transduction and affects the expression of genes regulating cell division,leading to dwarfism of *** study provides a new insight for the mechanism of rapeseed plant height regulation and creates an elite germplasm that can be used for genetic improvement of rapeseed architecture.

关键词： Brassica napus Plant height Brassinosteroids BIN2

全日制远程教学有效促进边远、民族地区教育发展的成因与启示

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中国电化教育 2016年第12期 59-66页

作者：张杰夫中国教育科学研究院北京100088

为加快边远、民族地区教育发展,寻找其有效发展途径,通过访谈、问卷调查、质性研究等方法,对我国开展的全日制远程教学进行了深入调查研究。全日制远程教学通过卫星、网络等信息技术,将城市优秀教师智慧辐射到边远、民族地区,为该地区... 详细信息

为加快边远、民族地区教育发展,寻找其有效发展途径,通过访谈、问卷调查、质性研究等方法,对我国开展的全日制远程教学进行了深入调查研究。全日制远程教学通过卫星、网络等信息技术,将城市优秀教师智慧辐射到边远、民族地区,为该地区学生成长和教师专业发展开辟出新的天地,有效地解决了困扰我国多年的偏远地区教育发展中的问题。其成功经验带来许多启示,边远、民族地区教育要实现大的发展,关键在于敢于突破"梯度理论"束缚,树立学生自信,引入城市巨大发展动力,名校带领薄弱校发展。全日制远程教学已经探索出一条利用信息技术促进边远、民族地区优质教育资源共享、实现跨越式发展的新路。

关键词：边远民族地区教育远程教学跨越式发展

视觉文化时代动漫的育人价值研究——基于小学生动漫活动现状调查

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教育研究 2014年第10期35卷 112-118页

作者：张杰夫中国教育科学研究院

随着人类加速走进视觉文化时代,动漫作为视觉语言,已经成为影响儿童学习和成长的重要媒介。动漫契合儿童的心理特征,具有巨大的教育潜力,它有利于儿童构建学习和成长的新环境,为儿童提供了观察学习的新路径和独特视角,有可能成为儿童学... 详细信息

随着人类加速走进视觉文化时代,动漫作为视觉语言,已经成为影响儿童学习和成长的重要媒介。动漫契合儿童的心理特征,具有巨大的教育潜力,它有利于儿童构建学习和成长的新环境,为儿童提供了观察学习的新路径和独特视角,有可能成为儿童学习的一种新的语言,有助于开发儿童右脑、培养创新人才。应利用动漫媒介培养创新人才,将动漫作为一种教育途径和工具与学校课程深度融合,营造动漫校园文化环境。

关键词：视觉文化动漫创新人才动漫语言

Identification of genes differentially expressed in female sterile mutant FS-M1 inBrassica napus by suppressive subtractive hybridization and microarray analysis

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Identification of genes differentially expressed in female s...

Identification and physical mapping of QTLs associated with flowering time in Brassica napus L.

江苏省遗传学会2016年学术年会

作者： Sanxiong Fu Xiaoying Zhou Song Chen jiefu zhang Nanjing Sub-Center(Rapeseed) of National Center of Oilseeds Crop Improvement Key Laboratory of Cotton and Rapeseed (Nanjing)Ministry of AgricultureP.R.ChinaInstitute of Industrial CropsJiangsu Academy of Agricultural Sciences

The female sterile mutant FS-M1 was isolated from a spontaneous mutation of Brassica napus *** the mutant is crossed as a female,very poor seed set is obtained,whereas it is fertile as a pollen *** floret of the mutant consisted of almost equal-length stamens,a short pistil,a flat style and ovary,and the stigma was *** order to screen the genes involved in embryogenesis and flower development,the suppressive subtraction hybridization(SSH) and microarray techniques were meanwhile *** differential expressing cDNA libraries were constructed with the c DNAs of the mutant FS-M1 as driver and the c DNAs of its wild type Ningyou10 as tester,vice *** recombined efficiencies of the forward and backward subtractive library were 96.2% and 94.5%,*** inserted fragment size ranged from 100 bp to 1000 bp,with an average size of about 500 bp.768 clones in the forward and backward subtractive library were screened by dot blot *** hundred positive clones of forward library and 134 positive clones of backward library were *** sequencing the clones expressing differentially in two cultivars and blasting their sequences,some ESTs regulating the embryogenesis,such as SAMDC,SLSG-6,and SLR1 were obtained from forward *** ESTs regulating flower development,such as YAB5,MYB,and CRABS CLAW(CRC) transcription factor were also obtained from backward *** Brassica microarray(4×44 K)was also used to analyze the gene differential expression of the female sterile mutant FS-M,Transcript levels of 3733 genes with significant differential expression levels(P < 0.05) were altered twofold or more for the female sterile mutant *** the 3733 genes,1872 were repressed and 1861 were ***-time reverse transcription PCR assays of eight of these 3733 genes confirmed the microarray *** important genes,such as Beta-1,3-glucanase,papillar cell-specific pectin methylesterase-like protein and AP2-lik

关键词： Brassica napus L. Suppressive subtraction hybridization Agilent Brassica microarray Female sterile mutant

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Identification and physical mapping of QTLs associated with ...

Indoor Propagation of Transgenic Glyphosate-tolerant Rapeseed (Brassica napus L.) and Transfer Breeding for Herbicide Tolerance

江苏省遗传学会2019年学术研讨会

作者： Kunjiang Yu Xiaodong Wang Feng Chen Wei zhang jiefu zhang Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences

Flowering time regulation is crucial to yield of rapeseed（Brassica napus L.）. However, insight into the loci controlling flowering time variation in rapeseed is not clear enough. In this study, a recombinant inbred line population was used for quantitative trait locus（QTL） analysis associated with flowering time in five different environments. A total of 36 identified QTLs were obtained, and each explained 3.96–24.41% of the phenotypic variation（PV）. The QTL meta-analysis showed that 36 identified QTLs were integrated into 24 consensus QTLs. Of these, eight consensus QTLs were stably expressed in at least two environments, and four with PV ≥ 10% were specifically expressed in a single environment. These 12 consensus QTLs were considered the key QTLs controlling flowering time in the AH population. Physical mapping indicated that the 12 key QTLs were mapped into 12 single genomic fragments in B. rapa and B. oleracea, and physical positions of these QTLs in the B. napus genome were further determined by referring to the B. rapa and B. oleracea genomes. Ultimately, seven candidate genes underlying the confidence intervals of five key QTLs were identified. These findings will contribute to further insight into the genetic architecture of flowering time in rapeseed.

关键词： Brassica napus L Flowering time QTL Physical mapping

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Agricultural Biotechnology 2021年第1期10卷 1-5页

作者： Xiaoying ZHOU Yaoyao WU Qi PENG Sanxiong FU Wei zhang Hongli ZHU jiefu zhang Song CHEN Institute of Industrial Crops Jiangsu Academy of Agricultural SciencesNanjing 210014China Key Laboratory of Cotton and Rapeseed(Nanjing) Ministry of AgricultureNanjing 210014China Suzhou Polytechnic Institute of Agriculture Suzhou 215008China

[Objectives] This study was conducted to investigate the possibility of screening for herbicide resistance in the early stage of rapes growth. [Methods] Eight treatments were set for a herbicide concentration experiment. [Results] Rape sprouts were highly sensitive to the herbicide glyphosate, and even a very low concentration of glyphosate solution(187 mg/L) was sufficient to kill most of the rape seedlings, while the genetically modified herbicide-tolerant rape could tolerate higher concentrations of the herbicide. Low concentrations of glyphosate could be used for screening at the sprout stage, and the results of herbicide-tolerance screening were consistent with PCR testing. [Conclusions] This study lays a technical and material foundation for accelerating the cultivation of new herbicide-resistant rape varieties suitable for rapeseed production areas in the lower reaches of the Yangtze River.

关键词： Transgenic herbicide-tolerant rape Indoor generation adding Transfer breeding Inheritance

Two SNP mutations of two BnFad2 homologue genes jointly determine the high oleic acid trait in rapeseed

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Two SNP mutations of two BnFad2 homologue genes jointly dete...