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Selenium-induced Changes in Activities of Antioxidant Enzymes and Content of Photosynthetic Pigments in Spirulina platensis

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Journal of Integrative Plant Biology 2008年第1期50卷 40-48页

作者： Tian-Feng Chen wen-jie zheng Yum-Shing Wong Fang Yang Department of Biology The Chinese University of Hong Kong Hong Kong SAR China Department of Chemistry Jinan University Guangzhou 510632 China

Spirulina platensis exposed to various selenium （Se） concentrations （0, 10, 20, 40, 80, 150, 175, 200, 250 mg/L） accumulated high amounts of Se in a dose- and time-dependent manner. Under low Se concentrations （〈150 mg/L）, Se induced increases in biomass concentration, content of photosynthetic pigments, and activities of glutathione peroxidase （GPX）, superoxide dismutase （SOD）, catalase （CAT） and Gua-dep peroxidases （POD）, which indicates that antioxidant enzymes play an important role in protecting cells from Se stress. Higher Se concentrations （≥175 mg/L） led to higher Se accumulation and increases in activities of GPX, SOD, CAT and POD, but also induced lipid peroxidation （LPO） coupled with potassium leakage and decreases in biomass concentration and contents of photosynthetic pigment. The results indicate that increases in activities of the antioxidant enzymes were not sufficient to protect cell membranes against Se stress. Time-dependent variations in the activities of antioxidant enzymes, contents of chlorophyll a and carotenoid and the LPO level were also investigated under representative Se concentrations of 40 and 200mg/L. Opposite variation trends between SOD-CAT activities, and GPX-POD-APX activities were observed during the growth cycles. The results showed that the prevention of damage to cell membranes of S. platensis cells could be achieved by cooperative effects of SOD-CAT and GPX-POD-APX enzymes. This study concludes that S. platensis possessed tolerance to Se and could protect itself from phytotoxicity induced by Se by altering various metabolic processes.

关键词： accumulation antioxidant enzymes photosynthetic pigments selenium Spirulina platensis.

Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation

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Hepatobiliary & Pancreatic Diseases International 2020年第5期19卷 449-454页

作者： wen-Li Sai Min Yao Shui-jie Shen wen-jie zheng Jian-Ying Sun Meng-Na Wu Li Wang Deng-Fu Yao Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityNantong 226001China Departments of Medical Immunology&Medical Informatics Medical College of Nantong UniversityNantong 226001China Department of Oncology Nantong Hospital of Traditional Chinese MedicineNantong 226001China Department of Oncology Affiliated Hospital of Nantong UniversityNantong 226001China

Background:Hepatic Golgi protein-73(GP73)expression is related to hepatocellular carcinoma(HCC)*** aim of this study was to investigate the dynamic expression of GP73 mRNA and protein during hepatocytes malignant ***:Human GP73 expressions in 88 HCC tissues and their self-control surrounding tissues were examined by immunohistochemistry,and survival time of HCC patients was evaluated by the Kaplan-Meier *** model of Sprague-Dawley rats was made by diet containing *** rats were divided into the control,hepatocyte degeneration,precanceration,and HCC groups to observe GP73 protein and mRNA alterations during hepatocytes malignant ***:The GP73 expression was significantly higher in the cancerous tissues than that in the surrounding tissues,with shorter survival time,and the positive rates of GP73 protein in human HCC tissues were 53.3%at stage I,84.0%at stage II,84.6%at stage III,and 60.0%at stage IV,*** positive rates of hepatic GP73 protein and mRNA in the rat models were none in the control group,66.7%and 44.4%in the hepatocytes degeneration group,88.9%and 77.8%in the hepatocytes precanceration group,and 100%in the HCC group,*** was a positive correlation(r=0.91,P<0.01)between hepatic GP73 and serum GP73 during rat hepatocytes malignant ***:Abnormal GP73 expression may be a sensitive and valuable biomarker in hepatocarcinogensis.

关键词： Golgi protein-73 Hepatocellular carcinoma Dynamic alteration Early prediction Biomarkers

Abnormal CD44 activation of hepatocytes with nonalcoholic fatty accumulation in rat hepatocarcinogenesis

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World Journal of Gastrointestinal Oncology 2020年第1期12卷 66-76页

作者： Miao Fang Min Yao jie Yang wen-jie zheng Li Wang Deng-Fu Yao Medical School of Nantong University Nantong 226001Jiangsu ProvinceChina Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Medical Informatics Medical School of Nantong UniversityNantong 226001Jiangsu ProvinceChina

BACKGROUND Prevalence of nonalcoholic fatty liver disease(NAFLD)is rapidly increasing,and NAFLD has become one of the most common chronic liver diseases *** abnormal CD44 activation,the severe form of NAFLD can progress to liver cirrhosis and hepatocellular carcinoma(HCC).Thus,the molecular mechanism of CD44 in NAFLD needs to be *** To investigate the relationship between CD44 activation and malignant transformation of rat hepatocytes under nonalcoholic lipid *** Sprague-Dawley rats were fed a high-fat(HF)for 12 wk to entice NAFLD and then with HF plus 2-fluorenylacetamide(0.05%)to induce *** were sacrificed every 2 wk,and subsequently divided into the groups based on liver pathological examination(hematoxylin and eosin staining):NAFLD,denaturation,precancerosis,HCC,and *** CD44 mRNA was detected by *** fat as assessed by Oil red O staining or CD44 by immunohistochemical assay was compared with their integral optic *** CD44,alanine aminotransferase,aspartate aminotransferase,triglyceride,total cholesterol,and AFP levels were quantitatively *** Elevated CD44 was first reported in hepatocarcinogenesis,with increasing expression from NAFLD to HCC at the protein or mRNA *** CD44 integral optic density values were significantly different between the control group and the NAFLD(t=25.433,P<0.001),denaturation(t=48.822,P<0.001),precancerosis(t=27.751,P<0.001),and HCC(t=16.239,P<0.001)groups,*** CD44 can be secreted into the blood,and serum CD44 levels in HCC or precancerous rats were significantly higher(P<0.001)than those in any of the other *** correlations were found between liver CD44 and CD44 mRNA(rs=0.373,P=0.043)and serum CD44(rs=0.541,P=0.002)and between liver CD44 mRNA and serum CD44(rs=0.507,P=0.004).Moreover,significant correlations were found between liver CD44 and liver AFP(rs=0.572,P=0.001),between serum CD44 and serum AFP(rs=0.608,P<0.001),an

关键词： Hepatocarcinogenesis CD44 Nonalcoholic fatty liver disease Animal model Dynamic expressions

Constitutive Modeling for Flow Behaviors of Superaustenitic Stainless Steel S32654 during Hot Deformation

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Journal of Iron and Steel Research International 2016年第2期23卷 178-184页

作者： En-xiang PU Han FENG Min LIU wen-jie zheng Han DONG Zhi-gang SONG Central Iron and Steel Research Institute Beijing 100081China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

Hot deformation behavior of superaustenitic stainless steel S32654 was investigated with hot compression tests at temperatures of 950-1250 C and strain rates of 0. 001-10 s-1. Above 1150 ℃, with strain rate lower than 0.1 s -1 , the flow curves exhibit nearly steady state behavior, while at higher strain rate, continuous flow softening occurs. To provide a precise prediction of flow behavior for the alloy, the constitutive modeling considering effect of strain was derived on the basis of the obtained experimental data and constitutive relationship which incorporated Ar- rhenius term and hyperbolic sine type equation. The material constants α, n, Q and lnA are found to be functions of the strain and can be fitted employing eighth-order polynomial. The developed constitutive model can be employed to describe the deformation behavior of superaustenitic stainless steel S32654.

关键词： S32654 superaustenitic stainless steel hot deformation constitutive modeling

Grain Growth Behavior of Inconel 625 Superalloy

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Journal of Iron and Steel Research International 2016年第10期23卷 1111-1118页

作者： Min LIU wen-jie zheng Jin-zhong XIANG Zhi-gang SONG En-xiang PU Han FENG School of Physics Science and Technology Yunnan University Kunming 650093 Yunnan China Central Iron and Steel Research Institute Beijing 100081 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China

The grain growth（GG）behavior of Inconel 625 superalloy was studied in the temperature range of 900-1 250 ℃and holding time range of 10-80 *** of the alloy were characterized by optical metallography,scanning electron microscopy（SEM）and transmission electron microscopy（TEM）.Grains grew obviously with either increasing temperature or extending holding time at temperatures above 1 050℃.However,at temperatures lower than 1 050 ℃,the GG was sluggish due to the pinning effect of carbide particles on grain boundary（GB）.Threshold temperature for transition from mixed grain structure to uniform one was considered to be around 1 100℃.Once the temperatures surpassed 1 200℃,an instant increase in the grain size occurred showing no dependence on holding *** analysis showed that the dominant second phase formed heterogeneously on the GB was M6 C,which significantly impeded grain *** the basis of experimental data,the mathematical model of GG was established,which can describe GG behavior of Inconel 625 alloy during solution treatment（ST）at 1 100-1 250 ℃.The activation energy for GG of Inconel 625 alloy was 207.3kJ,which suggested that the GG of Inconel 625 alloy was controlled by the process of GB diffusion.

关键词： Inconel 625 alloy growth kinetics solution treatment precipitation

Oncogenic tuftelin 1 as a potential molecular-targeted for inhibiting hepatocellular carcinoma growth

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World Journal of Gastroenterology 2021年第23期27卷 3327-3341页

作者： Meng-Na Wu wen-jie zheng wen-Xin Ye Li Wang Ying Chen jie Yang Deng-Fu Yao Min Yao Research Center of Clinical Medicine The Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Medical Immunology Medical School of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Medical Informatics Medical School of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Oncology The Affiliated Second Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Molecular Biology Life Science School of Nantong UniversityNantong 226009Jiangsu ProvinceChina

BACKGROUND Abnormal tuftelin 1(TUFT1)has been reported in multiple cancers and exhibits oncogenic roles in tumor ***,limited data are available on the relationship between TUFT1 and hepatocellular carcinoma(HCC),and the exact biological mechanism of TUFT1 is still poorly understood in *** To investigate TUFT1 expression in HCC and how interfering TUFT1 transcription affects HCC *** TUFT1 in HCC and non-HCC tissues based on databases of the Cancer Genome Atlas and Oncomine were analyzed,and TUFT1 in human HCC tissues on microarray were detected by immunohistochemistry for clinicopathological features,overall survival,and disease-free *** cells were transfected with constructed vectors of TUFT1 that interfere or over-express TUFT1 for analyzing the biological behaviors of HCC ***,invasion,migration,and apoptosis of cells were detected by cell counting kit-8,scratch assay,transwell tests,and flow cytometry and confirmed by Western blotting,*** Abnormal TUFT1 levels in databases expressed in HCC at messenger RNA(mRNA)level and HCC tissues were mainly located in cytoplasm and *** level of TUFT1 expression in the HCC group was significantly higher(χ2=18.563,P<0.001)than that in the non-cancerous group,closely related to clinical staging,size,vascular invasion of tumor,hepatitis B e-antigen positive,and ascites(P<0.01)of HCC patients,and negatively to HCC patients’overall survival and disease-free survival(P<0.001).After interfering with TUFT1 transcription at mRNA level in the MHCC-97H cells by the specific TUFT1-short hairpin RNA,cell proliferation,invasion,and metastasis were significantly inhibited with increasing apoptosis *** contrast,proliferation,invasion,and migration were significantly enhanced after over-expression of TUFT1 mRNA in Hep3B cells in *** Oncogenic TUFT1 was associated with the progression of HCC and could be a potential molecular-target for inhibiting

关键词： Hepatocellular carcinoma Tuftelin 1 Prognosis Molecular-target Growth

Optimization of Hot Workability in Superaustenitic Stainless Steel 654SMO

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Journal of Iron and Steel Research International 2014年第10期21卷 975-982页

作者： En-xiang PU wen-jie zheng Zhi-gang SONG Jin-zhong XIANG Xian-ping WEI School of Physics Science and Technology Yunnan University Central Iron and Steel Research Institute

Hot compression tests were conducted on a Gleeble-3800 machine in a temperature range of 950 to 1200 ℃ and a strain rate range of 0. 001 to 10 s-1 in order to study the hot deformation behaviour of superaustenitic stainless steel 654SMO. The results show that peak stress increases with decreasing temperature and increasing strain rate, and the apparent activation energy of this alloy was determined to be about 494 kJ/mol. The constitutive equation which can be used to relate the peak stress to the absolute temperature and strain rate was obtained. The processing maps for hot working developed on the basis of flow stress data and the dynamic materials model were adopted to op- timize the hot workability. It is found that the features of the maps obtained in the strain range of 0.2 to 1.0 are fun- damentally similar, indicating that the strain does not have a substantial influence on processing map. The combina- tion of processing map and mierostructural observations indicates that the favorable hot deformation conditions are located in two domains of processing map. The first domain occurs in the temperature range of 980 to 1035 ℃ and strain rate range of 0. 001 to 0.01 s-1 with a peak efficiency of 55%. The second domain appears in the temperature range of 1 120 to 1 180 ℃ and strain rate range of 0.3 to 3 s-1 with peak efficiency of 35%. Compared to other stable domains, the specimens deformed in these two domains exhibit full dynamic recrystallization grains with finer and more uniform sizes. An instability domain occurs at temperatures below 1 100 ℃ and strain rate above 0.1 s-1 , and flow instability is manifested in the form of flow localization.

关键词： 654SMO steel superaustenitic stainless steell processing mapl hot deformationl dynamic recrystallization

Induction of Endothelial Cell Apoptosis by Anti-alpha-enolase Antibody

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Chinese Medical Sciences Journal 2011年第3期26卷 152-157页

作者： Hong-bo Yang wen-jie zheng Xuan Zhang Fu-lin Tang Department of Endocrinology Peking Union Medical College HospitalChinese Academy of Medical Sciences ＆ Peking Union Medical CollegeBeijing 100730China Department of Rheumatology Peking Union Medical College HospitalChinese Academy of Medical Sciences ＆ Peking Union Medical CollegeBeijing 100730China

Objective To assess the prevalence of anti-alpha-enolase antibody in systemic autoimmune diseases in Chinese patients and its role in endothelial cell *** The reactivity of anti-alpha-enolase antibody in a variety of autoimmune disorders in Chinese patients was evaluated by dot blot *** cell apoptosis was investigated by in vitro incubation of endothelial cells with IgG purified from anti-alpha-enolase antibody-positive sera,with or without pre-incubation with recombinant *** Anti-alpha-enolase antibody was prevalent in different systemic autoimmune diseases with relatively high reactivity in Chinese *** vitro incubation of endothelial cells with IgG containing anti-alpha-enolase antibody induced apoptosis in a time-and dose-dependent *** was partly inhibited by pre-incubation of the endothelial cells with recombinant *** Our data suggest that alpha-enolase is a common auto-antigen recognized by anti-endothelial cell antibodies in connective tissue *** between alpha-enolase and its autoantibody plays a role in endothelial cell *** other than cell killing may contribute to the pathogenesis of endothelial damage and microvascular lesions.

关键词： anti-alpha-enolase antibody apoptosis endothelial cell

Effects of Carbon Content on Mechanical Properties of Inconel 718 Alloy

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Journal of Iron and Steel Research International 2015年第1期22卷 78-83页

作者： wen-jie zheng Xian-ping WEI Zhi-gang SONG Qi-long YONG Han FENG Qing-cheng XIE Central Iron and Steel Research Institute Materials Research Center Dong Fang Steam Turbine Works No.704 Institute of China Shipbuilding Industry Corporation

For improving the service performance of Inconel 718 alloy, especially used as a corrosion-resistant alloy for special environment, the microstructure and mechanical property of different carbon-containing Inconel 718 alloys were investigated by the Thermo-Calc software and experiments. The experimental results indicated that the mor- phology, distribution and types of carbides mainly existing in the form of MC＇were hardly influenced by solution treatment at 1050 ℃ for 1 h. The precipitation amount and particle size of carbides decreased with the decrease of carbon content, which was the main reason resulting in the increase of ductility and toughness. In addition, moving dislocation could be restrained by the precipitation of carbides. Therefore, the strength could benefit from the precip- itation strengthening of carbides when the precipitation of γ′/γ″ phase was not influenced by the precipitation of carbides.

关键词： Inconel 718 alloy microstructure carbide mechanical property precipitation strengthening