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Arenobufagin is a novel isoform-specific probe for sensing human sulfotransferase 2A1

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Acta Pharmaceutica Sinica B 2018年第5期8卷 784-794页

作者： Xiangge Tian Chao Wang Peipei Dong Yue An Xinyu Zhao Weiru Jiang Gang Wang jie Hou Lei Feng Yan Wang Guangbo ge Xiaokui huo Jing Ning Xiaochi Ma Academy of Integrative Medicine College of Pharmacy College of Basic Medical Science Second Affliated Hospital Dalian Medical University Institute of Functional Materials and Molecular Imaging College of Emergency and Trauma Hainan Medical University Institute of Functional Materials and Molecular Imaging College of Emergency and Traum1 Hainan Medical University Shanghai University of Traditional Chinese Medicine

Human cytosolic sulfotransferase 2 A1(SULT2A1) is an important phase II metabolic enzyme. The detection of SULT2A1 is helpful for the functional characterization of SULT2A1 and diagnosis of its related diseases. However, due to the overlapping substrate specificity among members of the sulfotransferase family, it is difficult to develop a probe substrate for selective detection of SULT2A1. In the present study, through characterization of the sulfation of series of bufadienolides, arenobufagin(AB) was proved as a potential probe substrate for SULT2A1 with high sensitivity and specificity. Subsequently, the sulfation of AB was characterized by experimental and molecular docking studies. The sulfate-conjugated metabolite was identified as *** sulfation of AB displayed a high selectivity for SULT2A1 which was confirmed by in vitro reaction phenotyping assays. The sulfation of AB by human liver cytosols and recombinant SULT2A1 both obeyed Michaelis-Menten kinetics, with similar kinetic parameters. Molecular docking was performed to understand the interaction between AB and SULT2A1, in which the lack of interaction with Met-137 and Tyr-238 of SULT2A1 made it possible to eliminate substrate inhibition of AB sulfation. Finally, the probe was successfully used to determine the activity of SULT2A1 and its isoenzymes in tissue preparations of human and laboratory animals.

关键词： Sulfotransferase 2A1 Sulfation Arenobufagin Probe Selective substrate

Simulation study on thermal performance of two turns helium cryogenic oscillating heat pipe for superconducting accelerator

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Radiation Detection Technology and Methods 2023年第3期7卷 427-434页

作者： Yi huo Changcheng Ma Jun Zhang Rui Huang Rui ge University of Chinese Academy of Sciences BeijingChina Institute of High Energy Physics BeijingChina Hefei University of Technology HefeiChina

Purpose In recent years,with the further development of superconducting technology,superconducting devices have been applied in particle accelerators and synchrotron radiation light source *** a new type of heat transfer structure,helium cryogenic oscillating heat pipe can be used to balance local hot spots in superconductors and improve heat transfer *** In this paper,a two-dimensional two-turns helium cryogenic oscillating heat pipe model is *** flow performance of helium cryogenic oscillating heat pipe was analyzed by CFD *** and conclusion The results show that the development of flow pattern in the helium cryogenic oscillating heat pipe first forms bubble flow under the heating of the evaporation section,and then gradually forms slug flow and annular *** process consists of initial stage,transition stage and running *** the running stage,the effective thermal conductivity of COHP oscillates around 15,000 W/(m·K).

关键词： Cryogenic oscillating heat pipe Helium CFD simulation Superconducting accelerator

免疫细胞介导不同脂质与膝骨关节炎的相关性:欧洲个体全基因组关联分析

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中国组织工程研究 2025年第18期29卷 3934-3940页

作者：霍将丁宇原杰山西医科大学第二医院骨科山西省太原市030001 山西省中西医结合医院山西省太原市030001

背景:观察性研究发现脂质与免疫细胞对于骨关节炎进展具有双重影响,但其具体作用机制尚不清楚。目的:探究脂质与膝骨关节炎的因果关系是否受免疫细胞调控。方法:179种脂质来源于Linda Ottensman数据库,从Open GWAS上获取到了731种免疫... 详细信息

背景:观察性研究发现脂质与免疫细胞对于骨关节炎进展具有双重影响,但其具体作用机制尚不清楚。目的:探究脂质与膝骨关节炎的因果关系是否受免疫细胞调控。方法:179种脂质来源于Linda Ottensman数据库,从Open GWAS上获取到了731种免疫细胞相关的单核苷酸多态性作为工具变量,膝骨关节炎的全基因组关联研究数据来自于骨关节炎遗传学联盟。首先采用双样本孟德尔随机化方法从遗传学角度分别探讨脂质与膝骨关节炎、免疫细胞与膝骨关节炎、脂质与免疫细胞的因果关系。另外采用贝叶斯加权孟德尔随机化验证脂质与膝骨关节炎的因果关系,反向孟德尔随机化和Steiger方向性检验评估正向关系。随后进行敏感性分析,包括Cochran’s Q检验消除异质性,MR-PRESSO全局检验和MR-Egger截距排除水平多效性,留一法评估单个单核苷酸多态性驱动因素对随机估计的影响。最后,通过两步孟德尔随机化方法确定特定免疫细胞性状在脂质与膝骨关节炎因果关系中的中介效应。结果与结论:①逆方差加权法和贝叶斯加权算法共同确定8种与膝骨关节炎存在因果关系的脂质种类;②Cochran’s Q、MR-PRESSO和MR-Egger截距结果均无统计学意义(P<0.05);③同时共计算得出了3组中介关系,介导比例最低为11.85%,最高为45.48%;④提示免疫细胞水平在脂质与膝骨关节炎的调节进程中发挥潜在作用,这一发现能为深入探索膝骨关节炎的作用机制提供新的视角。

关键词：脂质膝骨关节炎孟德尔随机化免疫细胞中介分析因果关系工程化组织构建

Expression Analysis of Restorer Alleles-Induced genes in Pepper

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Agricultural Sciences in China 2011年第7期10卷 1010-1015页

作者： GUO Shuang MA Ning YANG Wen-cai SUN Yu-jie SHEN huo-lin Department of Vegetable Science College of Agronomy and Biotechnology China Agricultural University Beijing 100193 P.R.China Guangzhou Academy of Agricultural Sciences Guangzhou 510308 P.R.China Faculty of Life Sciences Department of Agriculture and Ecology University of Copenhagen Hφbakkegaard Alle 13 2630 Taastrup Denmark

Fertility restoration of cytoplasmic male-sterility in pepper （Capsicum annuum L.） is useful for commercial production of hybrid seeds. However, the mechanism of fertility restoration has not been determined. We previously constructed a cDNA library and identified some genes related to fertility restoration in pepper using suppression subtractive hybridization technology. In this study, the expression patterns of 20 genes were investigated using semi-quantitative RT-PCR. Three genes expressed only in restorer lines, but not in sterility lines. Four genes expressed only in anther, but not in other organs. Among these 7 genes, the clone TG31 was observed to specifically express in anther of restorer lines. The work described here provides a comprehensive overview on the expression pattern of the genes that are induced by restorer alleles in pepper. It will also contribute to the current understanding of molecular networks for the regulation of fertility restoration.

关键词： pepper restorer alleles semi-quantitative RT-PCR expression

Experimental study on hypersonic crossflow instability over a swept flat plate by flow visualization

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Acta Mechanica Sinica 2021年第9期37卷 1395-1403,I0002页

作者： Hai-Bo Niu Shi-He Yi Jun-jie huo Wen-Peng Zheng Xiao-Lin Liu College of Aerospace Science and Engineering National University of Defense TechnologyChangsha410073China

An experimental study on the traveling crossflow instability over a 60∘swept flat plate was *** Mach number is 6,the angle of attack of the model is 5∘.The traveling crossflow waves and the secondary instability of the traveling crossflow waves were visualized by nano-tracer-based planar laser scattering(NPLS)*** the spanwise NPLS images,the traveling crossflow waves appeared as regular strikes,and the secondary instability appeared as small eddies attached to *** wavelet transform was used to study the wavelength contents of the traveling crossflow *** most amplified wavelength is stable before the secondary instability happening,which is around 12 mm at Re_(∞)=3.45×10^(6)m^(−1).Besides,the Reynolds number effects on the boundary layer transition and traveling crossflow instability were discussed.

关键词： Crossflow instability Swept flat plate Flow visualization Wavelet analysis Boundary layer transition

Diamines,CS2 and Monoisocyanide-participated Polymerizations for Large-scale Synthesis of Polythioureas and Thioformamide

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Chinese Journal of Polymer Science 2023年第10期41卷 1563-1576页

作者： jie Zhang Fan Ye Jin-Lei huo Jian-Wen Peng Rong-Rong Hu Ben Zhong Tang State Key Laboratory of Luminescent Materials and Devices Guangdong Provincial Key Laboratory of Luminescence from Molecular AggregatesSouth China University of TechnologyGuangzhou510640China Shenzhen Institute of Aggregate Science and Technology School of Science and EngineeringThe Chinese University of Hong KongShenzhenGuangdong518172China AIE Institute Guangzhou510530China

Multicomponent polymerizations(MCPs)are powerful tools to synthesize functional polymers with great structural diversity,low cost and high efficiency,which usually generate single polymer ***,a robust one-pot diamines,CS_(2)and monoisocyanide-participated catalyst-free polymerization was developed at room temperature to produce polythiourea and thioformamide simultaneously in equal equivalent,which was featured with cheap monomers,simple operation and mild condition,affording various polythioureas with high Mws of up to 4.75×10^(4)g/mol in high yields of up to 98%.Polythioureas with varied chain composition and sequence-controlled structure could be synthesized in 62 g-scale from copolymerization or multicomponent tandem polymerization,enabling facile tuning of thermal property,crystallinity,mechanical property,and *** abundant irregular hydrogen bonds endowed the polythioureas excellent glassy state self-healing property at room temperature or below 0℃.This polymerization provided an efficient and economic approach to access functional polythioureas.

关键词： CS_(2) Multicomponent polymerization Sequence-controlled polythiourea Scale-up synthesis Self-healing

Recent advances in nanofiltration,reverse osmosis membranes and their applications in biomedical separation field

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Chinese Journal of Chemical Engineering 2022年第9期35卷 76-99页

作者： Kai Zhang Huan-Huan Wu Hui-Qian huo Yan-Li Ji Yong Zhou Cong-jie Gao Center for Membrane and Water Science&Technology Zhejiang University of TechnologyHangzhou 310014China

In the face of human society's great requirements for health industry,and the much stricter safety and quality standards in the biomedical industry,the demand for advanced membrane separation technologies continues to rapidly grow in the ***(NF)and reverse osmosis(RO)as the highefficient,low energy consumption,and environmental friendly membrane separation techniques,show great promise in the application of biomedical separation *** chemical compositions,microstructures and surface properties of NF/RO membranes determine the separation accuracy,efficiency and operation cost in their ***,recent studies have focused on tuning the structures and tailoring the performance of NF/RO membranes via the design and synthesis of various advanced membrane materials,and exploring universal and convenient membrane preparation strategies,with the objective of promoting the better and faster development of NF/RO membrane separation technology in the biomedical separation *** paper reviews the recent studies on the NF/RO membranes constructed with various materials,including the polymeric materials,different dimensional inorganic/organic nanomaterials,porous polymeric materials and metal coordination polymers,***,the influence of membrane chemical compositions,interior microstructures,and surface characteristics on the separation performance of NF/RO membranes,are comprehensively ***,the applications of NF/RO membranes in biomedical separation field are systematically ***,the perspective for future challenges of NF/RO membrane separation techniques in this field is discussed.

关键词： Nanofiltration Reverse osmosis Membrane Separation Biomedical

An end-capped strategy for crystalline polymer donor to improve the photovoltaic performance of non-fullerene solar cells

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Science China Chemistry 2022年第5期65卷 964-972页

作者： Bing Zheng Yuchen Yue Jianling Ni Rui Sun jie Min Jingxia Wang Lei Jiang Lijun huo School of Chemistry Beihang UniversityBeijing 100191China Key Laboratory of Bioinspired Smart Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China The Institute for Advanced Studies Wuhan UniversityWuhan 430072China

The effects of end-capped modifications of a polymer donor with high molecular weight on non-fullerene solar cells are largely ignored,even if the chain-end-functionalized method of conjugated polymers is an effective strategy in modulating polymeric optical-electronic *** this study,we design and synthesize an end-capped polymer,PM6TPO,via a reaction with the parent polymer ***,the conventional detection methods of X-ray photoelectron spectroscopy(XPS),matrix-assisted laser desorption/ionization time-of-flight(MALDI-TOF),and ^(1)H nuclear magnetic resonance(^(1)H NMR) were replaced by simple solution-based inductively coupled plasma-mass spectrometry(ICP-MS) to evaluate the end-capped efficacy of *** introducing end-capped groups on a high molecular weight polymer donor,we could finely tune the aggregated behavior,strengthen the miscibility between the donor and acceptor without sacrificing the strong aggregated properties,and reduce the non-radiative recombination with a lower energy ***,the PM6TPO-based organic solar cell(OSC)realized a higher open-circuit voltage of 0.843 V and PCE of 17.26% than that of the non-end-capped parent polymer,PM6(0.824 V and 16.21%,respectively).This work not only provides a straightforward method for verifying the end-capped efficacy of a high molecular weight polymer but also indicates a new research direction for improving the photovoltaic performance of non-fullerene-based solar cells.

关键词： end-capped polymer ICP-MS miscibility energy loss non-fullerene solar cells

Tight Carbonate Microstructure and its Controls: A Case Study of Lower Jurassic Da'anzhai Member, Central Sichuan Basin

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Acta geologica Sinica(English Edition) 2020年第2期94卷 305-321页

作者： LI Bo WANG Xingzhi LIU Hongqi WANG Yongjun TIAN jie huo Fei GAO Zhaolong State Key Laboratory of Oil and Gas Reservoir geology and Exploitation Chengdu 610500China School of geoscience and Technology Southw est Petroleum UniversityChengdu 610500 China Research Institute of Petroleum Exploration&Development Beijing 100083China The Institute of Exploration and Development of Southwest Oil and Gas Field Branch.PetroChina.Chengdu 610059 China

Cast thin section observation, scanning electron microscopy(SEM), high-pressure mercury injection(HPMI), and nuclear magnetic resonance(NMR) were used to examine the microstructure of tight carbonate reservoirs in the Lower Jurassic Da’anzhai Member, the central Sichuan Basin. The pore space in the Da’anzhai Member is classified into 2 types and 17 subtypes, with nano-scale pore throats of ‘O’, ‘S’, ‘Z’, and ‘I’ shapes. Poorly sorted pore throats vary greatly in diameter;thus, it is difficult for fluid flow to pass through these pore throats. There are three classes of pore throats in carbonate reservoirs, i.e. isolated pores, pores coexisting with fractures, and large pores and fractures. Isolated pores may provide some pore space, but the permeability is low. Pores and fractures coexisting in the reservoir may have a great impact on porosity and permeability;they are the major pore space in the reservoir. Large pores and fractures have a great impact on reservoir properties, but they only account for a limited proportion of total pore space. The microstructure of Da’anzhai reservoirs, which dominates fluid mobility, is dependent on sedimentary environment, diagenesis, and tectonic process. Pore structure is related to sedimentary environment. The occurrence of microfractures, which may improve reservoir properties, is dependent on tectonic process. Diageneses are of utmost importance to pore evolution, cementation and growth of minerals have played an important role in destroying reservoir microstructure.

关键词： tight carbonate rocks microscopic pore structure diagenesis Da'anzhai Member Lower Jurassic Sichuan Basin