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Journal of Materials Science & Technology 2021年第9期68卷 140-146页

作者： Zhi-Jia Zhang Wei-jie li Shu-Lei Chou Chao Han Hua-Kun liu Shi-Xue Dou State Key Laboratory of Separation Membrane and Membrane Processes.Tianjin Municipal Key Laboratory of Advanced Fibers and Energy Storage School of Materials Science and EngineeringTiangong UniversityTianjin 300387China Institute for Superconducting and Electronic Materials University of WollongongWollongongNSW2522Australia School of Civil and Environmental Engineering Faculty of Engineering and Information TechnologyUniversity of Technology Sydney.SydneyNSW2007Australia

Red phosphorus/graphite(P/G) and red phosphorus/carbon nanotube(P/CNT) composites were prepared by ball milling red phosphorus with CNTs and graphite, respectively. The electrochemical results show superior electrochemical performances of the P/G and P/CNT composites compared with that of the reference sample milled with Super-P carbon. After 70 cycles, the P/G and P/CNT composites remained771.6 and 431.7 mA h g^(-1), with 68 % and 50 % capacity retention, respectively. With increasing the milling time(20 h), CNTs were cut into short pieces and then broken into carbon rings and sheets which were well mixed with red phosphorus. The morphology of the P/CNT composite can buffer the large volume changes from alloying and de-alloying during cycling, resulting in the enhanced cycling stability.

关键词： Carbon coating Red phosphorus Anode materials Sodium ion batteries

Low-Intensity Pulsed Ultrasound Promote Schwann Cells Pro-Myelination Activities and Neurite Outgrowth of Dorsal Root Ganglion Neurons

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Wuhan University Journal of Natural Sciences 2021年第3期26卷 278-288页

作者： JIANG Wenli WANG Yuexiang TANG jie WU Meng GUO Fajin li Shan li Mengpu Department of Ultrasound Beijing Hospital/National Center of GerontologyInstitute of Geriatric MedcineChinese Academy of Medical SciencesBeijing 100730China Department of Ultrasound Chinese PLA General HospitalBeijing 100853China Department of Ultrasound Zhongnan Hosptial of Wuhan UniversityWuhan 430071HubeiChina Department of Pediatric Tianjin HospitalTianjin UniversityTianjin 300200China

In this study,we investigate how Schwann cells and dorsal root ganglion(DRG)neurons response to direct low-intensity pulsed ultrasound(liPUS)stimuli in *** Schwann cells and DRG were isolated from rat sciatic nerve and spine,*** with varied dose of intensity(low:250 mW/cm2,medium:500 m W/cm2,high:750 m W/cm2)were applied 5 min per time for every other day,and pro-myelination indicators of Schwann cell as well as neurite outgrowth of dorsal root ganglion neurons were *** results demonstrated that liPUS promoted Schwann cells activity and proliferation from day 3 at the highest intensity,and day 5 at all *** addition,liPUS boosted pro-myelination activities of Schwann cells,as evidenced by increased cell population that positive for immunohistochemical staining against S100,nerve growth factor receptor(NGFR)p75,glial fibrillary acidic protein(GFAP),myelin protein zero(P0),as well as up-regulation of GFAP,Protein 0,nerve growth factor(NGF),and brain derived neurotrophic factor(BDNF)***,liPUS significantly enhanced the neurite outgrowth of DRG,with the highest intensity exhibiting longest neurite *** together,our results strongly improve the understanding of cellular mechanisms of ultrasonic therapies for peripheral nerve repair.

关键词： low-intensity pulsed ultrasound Schwann cell dorsal root ganglion peripheral nerve repair

Influence of Interfacial Enantiomeric Grafting on Melt Rheology and Crystallization of Polylactide/Cellulose Nanocrystals Composites

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Chinese Journal of Polymer Science 2022年第1期40卷 93-106页

作者： Hua-Gao Fang Kang-jie Yang Qi-Zheng Xie Xu Chen Sheng-li Wu Yun-Sheng Ding Department of Polymer Science and Engineering School of Chemistry and Chemical EngineeringHefei University of TechnologyHefei 230009China Anhui Province Key Laboratory of Advanced Functional Materials and Devices Hefei 230009China Anhui Institute for Food and Drug Control Hefei230051China

Incorporating the surface-grafted cellulose nanocrystals(CNCs)with enantiomeric polylactide(PLLA or PDLA)is an effective and sustainable way to modify PLLA,but their difference in promoting matrix crystallization is still *** this study,the CNCs with identical content and length of PLLA and PDLA(CNC-g-L and CNC-g-D)were prepared and blended with *** rheological properties of PLLA/CNC-gD are greatly improved,indicating that the stereocomplexation can significantly improve the interfacial strength as compared with the conventional van der Waals force in PLLA/***,the matrix crystallizes at a higher rate in PLLA/CNC-g-L than PLLA/***/CNC-g-L15 reaches its half crystallinity in 8.26 min while a longer period of 13.41 min is required for PLLA/*** observation reveals that the superior crystallization behavior in PLLA/CNC-g-L is originated from its higher nucleation efficiency and faster growth *** formation of low content of sc-PLA at the interface can restrict the diffusion of PLLA but contribute less to generate crystalline nuclei,which synergistically leads to the retarded crystallization kinetics in PLLA/*** the mechanism of different interfacial enantiomeric grafting on the melt rheology and crystallization of PLLA is of great significance for the development of high-performance polylactide materials.

关键词： Cellulose nanocrystal Polylactide nanocomposite Interfacial stereocomplexation Crystallization Rheology

Robust superconductivity and transport properties in (li_(1-x)Fe_x)OHFeSe single crystals

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Science China(Physics,Mechanics & Astronomy) 2016年第5期59卷 11-15页

作者： Hai lin jie Xing Xi Yu Zhu Huan Yang Hai-Hu Wen Center for Superconducting Physics and Materials National Laboratory of Solid State Microstructures and Department of PhysicsNational Collaborative Innovation Center of Advanced Microstructures Nanjing University

The recently discovered(li_(1-x) Fe_x)OHFe Se superconductor with T c about 40 K provides a good platform for investigating the magnetization and electrical transport properties of Fe Se-based superconductors. By using a hydrothermal ion-exchange method,we have successfully grown crystals of(li_(1-x) Fe_x)OHFe Se. X-ray diffraction on the sample shows the single crystalline Pb O-type structure with the c-axis preferential orientation. Magnetic susceptibility and resistive measurements show an onset superconducting transition at around T c=38.3 K. Using the magnetization hysteresis loops and Bean critical state model, a large critical current J s is observed in low temperature region. The critical current density is suppressed exponentially with increasing magnetic *** dependencies of resistivity under various currents and fields are measured, revealing a robust superconducting current density and bulk superconductivity.

关键词： iron chalcogenides critical currents magnetic properties transport properties

Cloning and identification of a novel RNF6 transcrip-tional splice variant Spg2 in human development

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Science China(life Sciences) 2008年第4期51卷 302-307页

作者： ZHU li TONG GuoQin CHEN jie WANG Yun WANG SaiQun ZHAO MuZhi li JianMin MA JianHua Lab of Reproductive Medicine Department of Cell Biology and Medical GeneticsNanjing Medical UniversityNanjing 210029China Department of Endocrinology Nanjing First HospitalThe Third Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China Dapartment of Obstetrics and Gynecology Nanjing Maternity and Child Health Care HospitalNanjing 210029China

RING finger proteins are zinc finger proteins containing the RING motifs. They act mainly as E3 ubiq-uitin ligases, bind the ubiquitin E2 conjugating enzyme and promote degradation of targeted proteins, Many novel genes have been isolated and differentially expressed in human adult and embryo testis by a testis cDNA-array differential display technique. A novel RING finger cDNA is highly expressed in adult testis and at low level in fetal testis. It was named Spg2. It contains a 2055 nucleotide ORF, en-codes a 685-amino-acid RNF6 protein, and has a RING finger in its C terminal. NCBI Blast shows that the gene is located on chromosome 13 and contains five exons. A multiple tissue expression profile also indicates that it is highly expressed in human testis, so we speculate that it may be associated with human spermatogenesis by virtue of the action of its RING domain.

关键词： alternative splice RING domain ubiquitination spermatogenesis

Interface Hydrogen-bonded Core-Shell Nanofibers by Coaxial Electrospinning

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Chinese Journal of Polymer Science 2017年第8期35卷 1001-1008页

作者： jing nie zhi-liang wang jie-fu li ying gong jia-xing sun 杨曙光 State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Center for Advanced Low-dimension Materials College of Material Science and Engineering Donghua University Shanghai 201620 China

Core-shell nanofibers were prepared by coaxial electrospinning technology,with poly（ethylene oxide）（PEO） as the core while poly（acrylic acid）（PAA） as the *** and PAA can form polymer complexes based on hydrogen *** order to avoid forming strong hydrogen bonding complexes at nozzle and blocking spinning process,a polar aprotic solvent,N,N-dimethylformamide（DMF）,was selected to dissolve PEO and PAA ***,TEM and DSC were utilized to characterize the morphology and structure of PEO-PAA core-shell *** spectra demonstrated that hydrogen bonding was formed at the core-shell *** addition,the PAA shell of the nanofibers can be cross-linked by ethylene glycol（EG） under heat treatment,which increases the stability and extends the potential applications in aqueous environment.

关键词： Coaxial electrospinning Core-shell nanofibers Hydrogen bonding

Protein identification and mRNA analysis of phyto-chrome-regulated genes in Arabidopsis under red light

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Science China(life Sciences) 2009年第4期52卷 371-380页

作者： li Xu YANG YueJun li Yan WANG jie XIAO XiaoJuan GUO XinHong TANG DongYing liU XuanMing Bioenergy and Biomaterial Research Center College of Life Science and BiotechnologyHunan UniversityChangsha 410082China

Phytochromes are a family of plant photoreceptors that mediate physiological and developmental re- sponses to red and far-red light. According to the affymetrix ATH1 microarray, phytochrome A (phyA) and phytochrome B (phyB) together play a key role in transducing the Rc signals to light-responsive genes. In order to select those red light-responsive genes associated with phyA or phyB, a proteomic approach based on two-dimensional gel electrophoresis (2-DE) was used to compare the protein ex- pression patterns of the phyAphyB double mutant and the wild type of Arabidopsis thaliana (col-4) which grew under constant red light conditions for 7 d. Thirty-two protein spots which exhibited dif- ferences in protein abundance were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. The expression of ten genes corresponding to ten protein spots was analyzed by a semiquantitative reverse transcription-polymerase chain reaction. Two of the ten genes were confirmed by quantitative PCR (Q-PCR). The results showed that phytochromes may exert their function by regulating mRNA or protein expressions. Proteomic analysis may provide a novel pathway for identifying phytochrome-dependent genes.

关键词： Arabidopsis thaliana phyAphyB Proteomic RT-PCR Q-PCR

A Biomimetic Surface for Infection-resistance through Assembly of Metal-phenolic Networks

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Chinese Journal of Polymer Science 2018年第5期36卷 576-583页

作者： Ru-Jian Jiang Shun-jie Yan li-Mei Tian Shi-Ai Xu Zhi-Rong Xin Shi-Fang Luan Jing-Hua Yin Lu-Quan Ren jie Zhao Key Laboratory of Bionic Engineering Ministry of Education Jilin University School of Chemistry and Chemical Engineering Yantai University State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences

Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a technical challenge. As a crucial structural component of biofilm, extracellular DNA（eDNA） can facilitate initial bacterial adhesion, subsequent development, and final maturation. Inspired by the mechanistic pathways of natural enzymes（deoxyribonuclease）, here we report a novel antibacterial surface by employing cerium（Ce（Ⅳ）） ion to mimic theDNA-cleavage ability of natural enzymes. In this process, the coordination chemistry of plant polyphenols and metal ions was exploited to create an in situ metal-phenolic film on substrate surfaces. Tannic acid（TA） works as an essential scaffold and Ce（Ⅳ） ion acts as both a cross-linker and a destructor of eDNA. The Ce（Ⅳ）-TA modified surface exhibited highly enhanced bacteria repellency and biofilm inhibition when compared with those of pristine or Fe（Ⅲ）-TA modified samples. Moreover, the easily produced coatings showed high stability under physiological conditions and had nontoxicity to cells for prolonged periods of time. This as-prepared DNA-cleavage surface presents versatile and promising performances to combat biomaterial-associated infections.

关键词： Antibacterial surface Metal-phenolic coating DNA-cleavage Biomimetic surface

Proteomics of serum exosomes identified fibulin-1 as a novel biomarker for mild cognitive impairment

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Neural Regeneration Research 2023年第3期18卷 587-593页

作者： Bo Chen li Song Juan Yang Wei-Ying Zhou Yuan-Yuan Cheng Yu-jie Lai College of Pharmacy Chongqing Medical UniversityChongqingChina Department of Neurology the First Affiliated Hospital of Chongqing Medical UniversityChongqing Key Laboratory of Major Neurological and Mental DisordersChongqing Key Laboratory of NeurologyChongqingChina Department of Neurology The Third Affiliated Hospital of Chongqing Medical UniversityChongqingChina

Mild cognitive impairment(MCI)is a prodrome of Alzheimer’s disease *** impairment patients often have a delayed diagnosis because there are no early symptoms or conventional diagnostic *** play a vital role in cell-to-cell communications and can act as promising biomarkers in diagnosing *** study was designed to identify serum exosomal candidate proteins that may play roles in diagnosing *** spectrometry coupled with tandem mass tag approach-based non-targeted proteomics was used to show the differentially expressed proteins in exosomes between MCI patients and healthy controls,and these differential proteins were validated using immunoblot and enzyme-linked immunosorbent *** of cognitive performance with the serum exosomal protein level was *** tracking analysis suggested that there was a higher serum exosome concentration and smaller exosome diameter in individuals with MCI compared with healthy *** identified 69 exosomal proteins that were differentially expressed between MCI patients and healthy controls using mass spectrometry ***-nine exosomal proteins were upregulated in MCI patients compared with those in control *** fibulin-1,with an area under the curve value of 0.81,may be a biomarker for an MCI *** exosomal protein signature from MCI patients reflected the cell adhesion molecule *** particular,higher exosomal fibulin-1 levels correlated with lower cognitive ***,this study revealed that exosomal fibulin-1 is a promising biomarker for diagnosing MCI.

关键词： Alzheimer’s disease biomarker diagnosis exosomes fibulin mass spectrometry mild cognitive impairment tandem mass tag cell adhesion molecule nanoparticle tracking analysis

C-type natriuretic peptide stimulates chicken myoblast differentiation through NPRB/NPRC receptors and metabolism pathway

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Journal of Integrative Agriculture 2022年第2期21卷 496-503页

作者： HUANG Hua-yun liANG Zhong liU Long-zhou li Chun-miao HUANG Zhen-yang WANG Qian-bao li Shou-feng ZHAO Zhen-hua Jiangsu Institute of Poultry Science Yangzhou 225125P.R.China College of Animal Science Yangtze UniversityJingzhou 8060550P.R.China

Skeletal muscle development is closely related with the amount of meat production and its quality in chickens. Natriuretic peptides(NPs) play an important role in myotube formation and fat oxidation of skeletal muscle in animals. The effect of C-type natriuretic peptide(CNP), an important member of the NPs, and its underlying molecular mechanisms in skeletal muscle are incompletely understood. Treatment of myoblasts with CNP led to enhanced proliferation/differentiation and significantly upregulated(Plic pathway, and six of them(phospholipase C β4, phospholipase C β2, phosphoglycerate mutase 1, creatine kinase B, peroxiredoxin 6 and CD38) were closely related to skeletal muscle development and differentially expressed. In conclusion, CNP stimulated differentiation of myoblasts by upregulating expression of the NPRB and NPRC receptors and enriching key genes in the metabolic pathway.

关键词： CNP NPRB/NPRC receptor myoblast differentiation metabolism pathway chicken