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Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway

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Journal of Integrative Medicine 2022年第2期20卷 163-172页

作者： Hong-xiao Li Ling Shi Shang-jie Liang Chen-chen Fang qian-qian Xu Ge Lu qian Wang jie Cheng jie Shen Mei-hong Shen College of Acupuncture Moxibustion and TuinaNanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina Key Laboratory of Acupuncture and Medicine Research of Ministry of Education Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina School of Traditional Chinese Medicine&School of Integrated Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina

Objective:Moxibustion,a common therapy in traditional Chinese medicine,has potential benefits for treating decreased ovarian reserve(DOR).The present study investigates the protective effect of moxibustion in a rat model of DOR and explores the possible mechanisms.Methods:Sixty-four female Sprague-Dawley rats were randomly divided into four groups:control,DOR,moxibustion(MOX),and hormone replacement therapy(HRT).The DOR rat model was established by intragastric administration of 50 mg/kg Tripterygium glycoside suspension(TGS),once daily for 14 days.MOX and HRT treatments were given from the day TGS administration was initiated.The ovarian reserve function was evaluated by monitoring the estrus cycle,morphological changes in ovaries,levels of serum estradiol(E2),follicle-stimulating hormone(FSH),luteinizing hormone(LH),and anti-Mullerian hormone(AMH),pregnancy rate and embryo numbers.Terminal-deoxynucleotidyl transferase-mediated nickend-labeling staining was used to identify ovarian granulosa cell apoptosis,while the protein and m RNA expressions of Bax,B-cell lymphoma-2(Bcl-2),phosphatidylinositol 3-kinase(PI3 K)and protein kinase B(AKT)in ovarian tissues were examined by immunohistochemistry,Western blot and quantitative reverse transcription-polymerase chain reaction.Results:Compared with the DOR group,MOX improved the disordered estrous cycle,promoted follicular growth,reduced the number of atresia follicles,increased the concentrations of serum E2 and AMH,and decreased serum FSH and LH concentrations.More importantly,the pregnancy rate and embryo numbers in DOR rats were both upregulated in the MOX treatment group,compared to the untreated DOR model.Further,we found that the MOX group had reduced apoptosis of ovarian granulosa cells,increased Bcl-2 expression and reduced expression of Bax.Furthermore,the PI3 K/AKT signaling pathway was triggered by the moxibustion treatment.Conclusion:Moxibustion improved ovarian function and suppressed apoptosis of ovarian granulosa cells in a rat model of DOR induced by TG

关键词： Decreased ovarian reserve Moxibustion Phosphatidylinositol 3-kinase Protein kinase B Apoptosis Infertility

Intelligent design and construction of novel APN-based theranostic probe driven by advanced computational methods

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Chinese Chemical Letters 2024年第2期35卷 352-357页

作者： Yingli Zhu Jie QIAN Kunqian Yu Jing Hou Yeshuo Ma Fei Chen jie Dong Wenbin Zeng Xiangya School of Pharmaceutical Sciences Central South UniversityChangsha 410083China National Engineering Research Center of Rice and Byproduct Deep Processing School of Food Science and EngineeringCentral South University of Forestry and TechnologyChangsha 410004China State Key Laboratory of Drug Research Drug Discovery and Design CenterShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Department of Geriatrics Third Xiangya HospitalCentral South UniversityChangsha 410013China

Multifunctional molecules with both optical signal and pharmacological activity play an important role in drug development,disease diagnosis,and basic theoretical research.Aminopeptidase N(APN),as a representative tumor biomarker with anti-tumor potential,still lacks a high-precision theranostic probe specifically targeting it.In this study,a novel quaternity design strategy for APN theranostic probe was developed.This proposed strategy utilizes advanced machine learning and molecular dynamics simulations,and cleverly employs the strategy of conformation-induced fluorescence recovery to achieve multi-objective optimization and integration of functional fragments.Through this strategy,a unique“Off-On”theranostic probe,ABTP-DPTB,was ingeniously constructed to light up APN through fluorescence restoration,relying on conformation-induced effects and solvent restriction.Differ from the common diagnostic probes,the intelligent design with non-substrated linkage makes ABTP-DPTB for long-term in-situ imaging.The fabricated probe was used for detecting and inhibiting APN in various environments,with a better in vitro inhibitory than golden-standard drug bestatin.

关键词： Theranostic probe Artificial intelligence Conformational restriction Fluorescence restoration Design strategy

Impact of subintimal plaque modification(SPM)technique on failed intervention of coronary artery chronic total occlusion

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Chronic Diseases and Translational Medicine 2021年第2期7卷 135-138页

作者： Na-Qiong Wu Jie QIAN Department of Endocrinology&Ccirdiometabolic Center National Center for Cardiovascular DiseasesFuwai HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100037China

To the editor:The intervention of chronic total occlusion(CTO)of coronary artery is the last castle to be conquered in coronary intervention.1 With the development of techniques and guidewires,even in high-volume centers,the failure rate of CTO is still 10%-15%.Vo MN et al2 described a“subintimal plaque modification”(SPM)that involves use of antegrade and retrograde dissection reentry techniques to treat“balloon-un-crossable”coronary lesion in 2014.The Subintimal Tracking and Reentry(STAR)technique is not suitable for routine use during CTO percutaneous coronary intervention(PCI).SPM is also called STAR without stenting,which can be used as a bailout strategy in CTO PCI when standard crossing attempts fail.Typically,SPM technique is performed as balloon dilation of the subintimal space through the CTO segment.

关键词： coronary balloon routine

Arabidopsis mitochondrial single-stranded DNA-binding proteins SSB1 and SSB2 are essential regulators of mtDNA replication and homologous recombination

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Journal of Integrative Plant Biology 2022年第10期64卷 1952-1965页

作者： Jie QIAN Min Zheng Lingyu Wang Yu Song Jiawen Yan Yi‐Feng Hsu School of Life Sciences Southwest UniversityChongqing 400715China

Faithful DNA replication is one of the most essential processes in almost all living organisms.However,the proteins responsible for organellar DNA replication are still largely unknown in plants.Here,we show that the two mitochondriontargeted single-stranded DNA-binding(SSB)proteins SSB1 and SSB2 directly interact with each other and act as key factors for mitochondrial DNA(mt DNA)maintenance,as their single or double loss-of-function mutants exhibit severe germination delay and growth retardation.The mt DNA levels in mutants lacking SSB1 and/or SSB2 function were two-to four-fold higher than in the wild-type(WT),revealing a negative role for SSB1/2 in regulating mt DNA replication.Genetic analysis indicated that SSB1 functions upstream of mitochondrial DNA POLYMERASE IA(POLIA)or POLIB in mt DNA replication,as mutation in either gene restored the high mt DNA copy number of the ssb1-1 mutant back to WT levels.In addition,SSB1 and SSB2 also participate in mitochondrial genome maintenance by influencing mt DNA homologous recombination(HR).Additional genetic analysis suggested that SSB1 functions upstream of ORGANELLAR SINGLE-STRANDED DNABINDING PROTEIN1(OSB1)during mt DNA replication,while SSB1 may act downstream of OSB1and MUTS HOMOLOG1 for mt DNA HR.Overall,our results yield new insights into the roles of the plant mitochondrion-targeted SSB proteins and OSB1 in maintaining mt DNA stability via affecting DNA replication and DNA HR.

关键词： Arabidopsis mitochondria mtDNA replication mtDNA recombination SSB

A high-safety active/passive hybrid control approach for compressor surge based on nonlinear model predictive control

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Chinese Journal of Aeronautics 2023年第1期36卷 396-412页

作者： Hanlin SHENG qian CHEN jie ZHANG Tianhong ZHANG Jiangsu Province Key Laboratory of Aerospace Power System College of Energy and Power EngineeringNanjing University of Aeronautics and AstronauticsNanjing 210016China

Surge active control can expand the stable operating range of the compressor.However,the difficulty of flow measurement,dynamic uncertainty disturbance,actuator delay characteristics,hard constraints of control variable,and system security measures have not been fully considered in the existing active control system,which significantly hinders its engineering application.Therefore,a nonlinear model predictive surge active control method is first presented based on flow estimator designed by using a continuous-time Kalman filter for dealing with the hard constraint of control variable and the impact of actuator delay of compression system with dynamic uncertainty.Then,a high-safety active/surge passive hybrid control strategy is designed,dominated by the surge active control and supplemented by the surge passive control,to ensure the compression system’s safe and stable operation.Lastly,the simulation results suggest that the flow estimator accurately estimates the compressor flow.When considering the delay impact of the actuators and sensors and measurement noise on the system,the proposed method exhibits stronger robustness than the existing meth-ods.The active/surge passive hybrid control strategy can successfully ensure the compression system’s safe and stable operation.This paper is of high practical significance for the engineering application of future compressor surge active control technologies.

关键词： Active control Compressor surge Flow estimator Hybrid system Model predictive control

Hyperactivity of the Lateral Septum Leads to Hypersensitivity in Susceptible Mice

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Neuroscience Bulletin 2023年第9期39卷 1466-1468页

作者： Fan qian Xia Zhang Bin Zhang jie Li Neuropsychiatry Research Institute The Affiliated Hospital of Qingdao UniversityQingdao266000China Department of Neurology West China Hospital of Sichuan UniversityChengdu610041China

Everyone needs social interactions,not only humans but also most species.Positive social interaction feedback is a reward,promoting more social behaviors and acting as a stress buffer.On the contrary,negative social interactions such as school bullying may become a type of stress that leads to mental illnesses including anxiety and depression disorders.Chronic social defeat stress(CSDS)is a widely accepted social trauma model in rodents based on repeated social attacks,following which the defeated mice can be classified as susceptible(SUS)and resilient(RES)according to their social performance.

关键词： stress illness contrary

Selection of suitable internal controls for gene expression normalization in rats with spinal cord injury

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Neural Regeneration Research 2022年第6期17卷 1387-1392页

作者： Wei Liu jie Yu Yi-Fan Wang qian-qian Shan Ya-Xian Wang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationNational Medical Products Administration(NMPA)Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Department of Nursing The Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Clinical Medicine Medical School of Nantong UniversityNantongJiangsu ProvinceChina Department of Radiotherapy and Oncology The Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In this study,we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes:Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s,Tbp,Ubc,Ubxn11,and Ywhaz.Our results show that the expression of Ankrd27,Ubc,and Tbp were stable after spinal cord injury,while Actb was the most unstable internal control gene.Ankrd27,Ubc,Tbp,and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression.Target gene expression levels and changes over time were similar when Ankrd27,Ubc,and Tbp were used as internal controls but different when Actb was used as an internal control.We recommend that Ankrd27,Ubc,and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research.This study was approved by the Administration Committee of Experimental Animals,Jiangsu Province,China(approval No.20180304-008)on March 4,2018.

关键词： geNorm analysis reference genes internal control genes normalization NormFinder analysis reverse transcription-quantitative polymerase chain reaction spinal cord injury stability of gene expression

Role of Mitophagy in Myocardial Ischemia/Reperfusion Injury and Chinese Medicine Treatment

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Chinese Journal of Integrative Medicine 2023年第1期29卷 81-88页

作者： XIA Jun-yan CHEN Cong LIN qian CUI jie WAN jie LI Yan LI Dong Department of Cardiology Dongfang Hospitalthe Second Affiliated Hospital of Beijing University of Chinese MedicineBeijing100078China Department of Cardiology Dongzhimen Hospitalthe First Affiliated Hospital of Beijing University of Chinese MedicineBeijing100007China

Mitophagy is one of the important targets for the prevention and treatment of myocardial ischemia/reperfusion injury(MIRI).Moderate mitophagy can remove damaged mitochondria,inhibit excessive reactive oxygen species accumulation,and protect mitochondria from damage.However,excessive enhancement of mitophagy greatly reduces adenosine triphosphate production and energy supply for cell survival,and aggravates cell death.How dysfunctional mitochondria are selectively recognized and engulfed is related to the interaction of adaptors on the mitochondrial membrane,which mainly include phosphatase and tensin homolog deleted on chromosome ten(PTEN)-induced kinase 1/Parkin,hypoxia-inducible factor-1α/Bcl-2 and adenovirus e1b19k Da interacting protein 3,FUN-14 domain containing protein 1 receptor-mediated mitophagy pathway and so on.In this review,the authors briefly summarize the main pathways currently studied on mitophagy and the relationship between mitophagy and MIRI,and incorporate and analyze research data on prevention and treatment of MIRI with Chinese medicine,thereby provide relevant theoretical basis and treatment ideas for clinical prevention of MIRI.

关键词： mitophagy myocardial ischemia/reperfusion injury PTEN-induced kinase 1/Parkin hypoxia-inducible factor-1α/Bcl-2 and adenovirus e1b19k Da interacting protein 3 FUN-14 domain containing protein 1 Chinese medicine

Software approaches for resilience of high performance computing systems:a survey

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Frontiers of Computer Science 2023年第4期17卷 43-56页

作者： jie JIA Yi LIU Guozhen ZHANG Yulin GAO Depei qian School of Computer Science and Engineering Beihang UniversityBeijing 100191China Sino-German Joint Software Institute Beihang UniversityBeijing 100191China

With the scaling up of high-performance computing systems in recent years,their reliability has been descending continuously.Therefore,system resilience has been regarded as one of the critical challenges for large-scale HPC systems.Various techniques and systems have been proposed to ensure the correct execution and completion of parallel programs.This paper provides a comprehensive survey of existing software resilience approaches.Firstly,a classification of software resilience approaches is presented;then we introduce major approaches and techniques,including checkpointing,replication,soft error resilience,algorithmbased fault tolerance,fault detection and prediction.In addition,challenges exposed by system-scale and heterogeneous architecture are also discussed.

关键词： resilience high-performance computing fault tolerance challenge