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Cytochrome P45026A1 Contributes to the Maintenance of Neuropathic Pain

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Neuroscience Bulletin 2024年第3期40卷 293-309页

作者： De-Li Cao ling-jie Ma Bao-Chun Jiang Qiang Gu Yong-Jing Gao Institute of Pain Medicine and Special Environmental Medicine Co-innovation Center of NeuroregenerationNantong UniversityNantong226019China Nantong University Medical School Nantong226001China Department of Pain Management The Affiliated Hospital of Nantong UniversityNantong226001China

The cytochrome P450 proteins(CYP450s)have been implicated in catalyzing numerous important biological reactions and contribute to a variety of ***26A1,a member of the CYP450 family,carries out the oxidative metabolism of retinoic acid(RA),the active metabolite of vitamin *** we report that CYP26A1 was dramatically upregulated in the spinal cord after spinal nerve ligation(SNL).CYP26A1 was mainly expressed in spinal neurons and *** analysis displayed that the content of all-trans-RA(at-RA),the substrate of CYP26A1,was reduced in the spinal cord on day 7 after *** of CYP26A1 by siRNA or inhibition of CYP26A1-mediated at-RA catabolism by talarozole relieved the SNL-induced mechanical allodynia during the maintenance phase of neuropathic *** also reduced SNL-induced glial activation and proinflammatory cytokine production but increased anti-inflammatory cytokine(IL-10)*** RA receptors RARα,RXRβ,and RXRγwere expressed in spinal neurons and glial *** promoter of Il-10 has several binding sites for RA receptors,and at-RA directly increased Il-10 mRNA expression in ***,intrathecal IL-10 attenuated SNL-induced neuropathic pain and reduced the activation of astrocytes and ***,the inhibition of CYP26A1-mediated at-RA catabolism alleviates SNL-induced neuropathic pain by promoting the expression of IL-10 and suppressing glial ***26A1 may be a potential therapeutic target for the treatment of neuropathic pain.

关键词： CYP26A1 Microglia Astrocytes ERK P38 IL-10 Neuropathic pain

Meibomian glands segmentation in infrared images with limited annotation

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 401-407页

作者： Jia-Wen Lin ling-jie Lin Feng Lu Tai-Chen Lai Jing Zou Lin-ling Guo Zhi-Ming Lin Li Li College of Computer and Data Science Fuzhou UniversityFuzhou 350108Fujian ProvinceChina Fujian Provincial Key Laboratory of Networking Computing and Intelligent Information Processing Fuzhou UniversityFuzhou 350108Fujian ProvinceChina Shengli Clinical Medical College of Fujian Medical University Fuzhou 350001Fujian ProvinceChina Department of Ophthalmology Fujian Provincial Hospital South BranchFujian Provincial HospitalFuzhou 350001Fujian ProvinceChina

●AIM:To investigate a pioneering framework for the segmentation of meibomian glands(MGs),using limited annotations to reduce the workload on ophthalmologists and enhance the efficiency of clinical diagnosis.●METHODS:Totally 203 infrared meibomian images from 138 patients with dry eye disease,accompanied by corresponding annotations,were gathered for the study.A rectified scribble-supervised gland segmentation(RSSGS)model,incorporating temporal ensemble prediction,uncertainty estimation,and a transformation equivariance constraint,was introduced to address constraints imposed by limited supervision information inherent in scribble *** viability and efficacy of the proposed model were assessed based on accuracy,intersection over union(IoU),and dice coefficient.●RESULTS:Using manual labels as the gold standard,RSSGS demonstrated outcomes with an accuracy of 93.54%,a dice coefficient of 78.02%,and an IoU of 64.18%.Notably,these performance metrics exceed the current weakly supervised state-of-the-art methods by 0.76%,2.06%,and 2.69%,***,despite achieving a substantial 80%reduction in annotation costs,it only lags behind fully annotated methods by 0.72%,1.51%,and 2.04%.●CONCLUSION:An innovative automatic segmentation model is developed for MGs in infrared eyelid images,using scribble annotation for *** model maintains an exceptionally high level of segmentation accuracy while substantially reducing training *** holds substantial utility for calculating clinical parameters,thereby greatly enhancing the diagnostic efficiency of ophthalmologists in evaluating meibomian gland dysfunction.

关键词： infrared meibomian glands images meibomian gland dysfunction meibomian glands segmentation weak supervision scribbled annotation

Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A

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Journal of Integrative Plant Biology 2024年第9期66卷 1915-1933页

作者： Miqi Xu Yi‐Yuan Wang Yujie Wu Xiuhong Zhou Ziyan Shan Kunying Tao Kaiqiang Qian Xuncheng Wang Jian Li Qingqing Wu Xing Wang Deng Jun‐jie ling National Engineering Laboratory of Crop Stress Resistance Breeding School of Life SciencesAnhui Agricultural UniversityHefei 230036China Province Key Laboratory of Resource Insect Biology and Innovative Utilization School of Life SciencesAnhui Agricultural UniversityHefei 230036China Biotechnology Center State Key Laboratory of Tea Plant Biology and UtilizationSchool of Tea and Food Sciences and TechnologyAnhui Agricultural UniversityHefei 230036China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Life Sciences Nanjing Normal UniversityNanjing 210023China State Key Laboratory of Protein and Plant Gene Research Peking–Tsinghua Center for Life SciencesSchool of Advanced Agriculture Sciencesand School of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Wheat Improvement Shandong Laboratory of Advanced Agricultural Sciences at WeifangPeking University Institute of Advanced Agricultural SciencesWeifang 261000China

Although green light(GL)is located in the middle of the visible light spectrum and regulates a series of plant developmental processes,the mechanism by which it regulates seedling development is largely *** this study,we demonstrated that GL promotes atypical photomorphogenesis in Arabidopsis thaliana via the dual regulations of phytochrome B(phyB)and *** the Pr-to-Pfr conversion rates of phyB and phyA under GL were lower than those under red light(RL)in a fluence rate-dependent and time-dependent manner,long-term treatment with GL induced high Pfr/Pr ratios of phyB and ***,GL induced the formation of numerous small phyB photobodies in the nucleus,resulting in atypical photomorphogenesis,with smaller cotyledon opening angles and longer hypocotyls in seedlings compared to *** abundance of phyA significantly decreased after short-and long-term GL *** determined that four major PHYTOCHROME-INTERACTING FACTORs(PIFs:PIF1,PIF3,PIF4,and PIF5)act downstream of phyB in GL-mediated cotyledon *** addition,GL plays opposite roles in regulating different *** example,under continuous GL,the protein levels of all PIFs decreased,whereas the transcript levels of PIF4 and PIF5 strongly increased compared with dark *** together,our work provides a detailed molecular framework for understanding the role of the antagonistic regulations of phyB and phyA in GL-mediated atypical photomorphogenesis.

关键词： cotyledon development green light photobody phytochrome PIF3

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梯度聚合增强对抗样本迁移性方法

计算机工程与应用 2024年第14期60卷 275-282页

作者：邓诗芸凌捷广东工业大学计算机学院广州510006

基于深度神经网络的图像分类模型容易受到对抗样本的攻击。现有研究表明,白盒攻击已经能够实现较高的攻击成功率,但在攻击其他模型时对抗样本的可迁移性较低。为提高对抗攻击的可迁移性,提出一种梯度聚合增强对抗样本迁移性方法。将原... 详细信息

基于深度神经网络的图像分类模型容易受到对抗样本的攻击。现有研究表明,白盒攻击已经能够实现较高的攻击成功率,但在攻击其他模型时对抗样本的可迁移性较低。为提高对抗攻击的可迁移性,提出一种梯度聚合增强对抗样本迁移性方法。将原始图像与其他类别图像以特定比例进行混合,得到混合图像。通过综合考虑不同类别图像的信息,并平衡各类别之间的梯度贡献,可以避免局部振荡的影响。在迭代过程中聚合当前点的邻域其他数据点的梯度信息以优化梯度方向,避免对单一数据点的过度依赖,从而生成具有更强迁移性的对抗样本。在ImageNet数据集上的实验结果表明,所提方法显著提高了黑盒攻击的成功率和对抗样本的可迁移性。在单模型攻击上,该方法在四种常规训练模型的平均攻击成功率为88.5%,相比Admix方法提升了2.7个百分点;在集成模型攻击上平均攻击成功率达到了92.7%。此外,该方法可以与基于转换的对抗攻击方法相融合,在三种对抗训练模型上平均攻击成功率相较Admix方法提高了10.1个百分点,增强了对抗攻击的可迁移性。

关键词：深度神经网络对抗攻击可迁移性梯度聚合

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基于稠密连接卷积网络的加密流量分类方法

计算机应用与软件 2024年第9期41卷 363-369页

作者：康健豪凌捷广东工业大学计算机学院广东广州510000

针对传统方法依靠人工提取特征、现有基于深度学习的方法在多分类上性能不够高等问题,提出一种基于稠密连接卷积网络的加密流量分类方法,利用DenseBlock中各层卷积稠密连接的结构,减轻梯度消失、加强特征传递,以提高分类性能。在公开数... 详细信息

针对传统方法依靠人工提取特征、现有基于深度学习的方法在多分类上性能不够高等问题,提出一种基于稠密连接卷积网络的加密流量分类方法,利用DenseBlock中各层卷积稠密连接的结构,减轻梯度消失、加强特征传递,以提高分类性能。在公开数据集“ISCX VPN-nonVPN”上进行实验,结果表明该方法对不同种类的加密流量有更好的分类效果,准确率达到98.56%,F1值达到98.55%,相比于基于一维卷积神经网络模型和ResNet模型的方法,准确率分别提升了8.88百分点和6.54百分点,F1值分别提升了8.86百分点和6.64百分点。

关键词：加密流量分类卷积神经网络稠密连接图像分类深度学习

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基于CNN-BiGRU的恶意域名检测方法

计算机应用与软件 2024年第6期41卷 336-341页

作者：林梓宇凌捷广东工业大学计算机学院广东广州510006

恶意域名检测对于防范僵尸网络等网络攻击具有重要意义。该文提出一种基于CNN和BiGRU的恶意域名检测方法CNN-BiGRU-Focal,利用卷积神经网络和双向门控循环单元网络来进行特征的融合学习,并引入改进的Focal Loss函数用以解决数据不平衡... 详细信息

恶意域名检测对于防范僵尸网络等网络攻击具有重要意义。该文提出一种基于CNN和BiGRU的恶意域名检测方法CNN-BiGRU-Focal,利用卷积神经网络和双向门控循环单元网络来进行特征的融合学习,并引入改进的Focal Loss函数用以解决数据不平衡问题。与LSTM、CNN、GRU、ATT-CNN-BiLSTM方法的对比实验表明,文章方法在多分类实验中检测准确率分别提高1.43百分点、2.89百分点、1.27百分点、2.43百分点,在二分类实验中检测准确率分别提高0.19百分点、0.12百分点、1.41百分点、0.3百分点。实验表明CNN-BiGRU-Focal方法在恶意域名的检测上有着更好的性能。

关键词：域名生成算法深度学习卷积神经网络双向门控循环单元网络

基于UPLC-Q-TOF MS鉴定复方香薷水化学成分

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分析测试学报 2024年第4期43卷 541-554页

作者：林结玲周熙赵昕遆慧慧广东药科大学药学院广东广州510006 广东省科学院测试分析研究所(中国广州分析测试中心) 广东省化学测量与应急检测技术重点实验室广东省中药质量安全工程技术研究中心广东广州510070

采用超高效液相色谱-四极杆串联飞行时间质谱(UPLC-Q-TOF MS)技术对中药复方香薷水的化学成分进行快速分析鉴定。通过色谱峰响应程度和分离效果优化复方香薷水的前处理方法和仪器条件;采用Agi⁃lent Phe Hex(150 mm×2.1 mm,1.9µm)色谱... 详细信息

采用超高效液相色谱-四极杆串联飞行时间质谱(UPLC-Q-TOF MS)技术对中药复方香薷水的化学成分进行快速分析鉴定。通过色谱峰响应程度和分离效果优化复方香薷水的前处理方法和仪器条件;采用Agi⁃lent Phe Hex(150 mm×2.1 mm,1.9µm)色谱柱进行分离,以0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱,柱温35℃,流速0.3 mL/min;通过正、负两种离子模式采集复方香薷水的质谱数据,得到精确质量数和二级碎片,结合对照品、Pubchem、安捷伦中药化合物质谱数据库和文献报道等信息对其化合物进行分析鉴定。鉴定出复方香薷水的112个成分,其中黄酮类22个,苯丙素类20个、有机酸18个、萜类14个、生物碱11个、氨基酸5个以及其他类型化合物22个。该研究全面阐述了复方香薷水的化学物质基础,为其质量控制和药效物质研究提供了理论依据。

关键词：超高效液相色谱-四极杆串联飞行时间质谱(UPLC-Q-TOF MS) 复方香薷水成分分析黄酮类

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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu ling-jie Zhu Xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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基于多窗口注意力机制的中文命名实体识别

小型微型计算机系统 2024年第6期45卷 1325-1330页

作者：占文韬吴晓鸰凌捷广东工业大学计算机学院广州510006

近年来,由于Transformer模型中应用的多头注意力机制能够有效地捕获较长的上下文信息,基于Transformer的模型已经成为主流方法.尽管这些模型能够有效捕获全局上下文信息,它们在局部特征和位置信息提取方面仍然有限.因此,本文提出了一种... 详细信息

近年来,由于Transformer模型中应用的多头注意力机制能够有效地捕获较长的上下文信息,基于Transformer的模型已经成为主流方法.尽管这些模型能够有效捕获全局上下文信息,它们在局部特征和位置信息提取方面仍然有限.因此,本文提出了一种基于多窗口注意力机制的中文命名实体识别模型.首先,通过基于Transformer的预训练语言模型RoBERTa把文本表征为字符级嵌入向量,捕捉深度上下文信息,得到全局上下文信息;其次,局部特征提取模块利用多窗口循环机制,在全局特征提取模块的引导下提取局部特征和位置信息;最后,所提出的多窗口注意力机制有效地融合全局特征和多个局部特征来预测实体标签.在CMeEE和MSRA数据集上进行了实验验证;结果表明,本文所提出的模型分别获得了64.31%和94.14%的F1值,性能优于其他同类模型,验证了其在中文命名实体识别的有效性.

关键词：命名实体识别多窗口注意力机制特征融合 RoBERTa