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N6-methyladenosine-modified circRIMS2 mediates synaptic and memory impairments by activating GluN2B ubiquitination in Alzheimer’s disease

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Translational Neurodegeneration 2023年第1期12卷 111-129页

作者： Xiong Wang jiazhao xie Lu Tan Yanjun Lu Na Shen Jiaoyuan Li Hui Hu Huijun Li Xiaoguang Li Liming Cheng Department of Laboratory Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Departments of Pathophysiology Guangxi Medical UniversityNanning 530021China Department of Pathophysiology School of Basic MedicineKey Laboratory of Education Ministry of China/Hubei Province for Neurological DisordersTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hepatic Biliary Pancreatic Surgery Department The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China Clinic Center of Human Gene Research Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Background Synaptic degeneration occurs in the early stage of Alzheimer’s disease(AD)before devastating symptoms,strongly correlated with cognitive *** RNAs(circRNAs)are abundantly enriched in neural tissues,and aberrant expression of circRNAs precedes AD symptoms,significantly correlated with clinical dementia ***,the direct relationship between circRNA dysregulation and synaptic impairment in the early stage of AD remains poorly *** Hippocampal whole-transcriptome sequencing was performed to identify dysregulated circRNAs and miRNAs in 4-month-old wild-type and APP/PS1 *** antisense purification and mass spectrometry were utilized to unveil interactions between circRIMS2 and methyltransferase 3,N6-adenosine-methyltransferase complex catalytic subunit(METTL3).The roles of circRIMS2/miR-3968 in synaptic targeting of UBE2K-mediated ubiquitination of GluN2B subunit of NMDA receptor were evaluated via numerous lentiviruses followed by morphological staining,co-immunoprecipitation and behavioral ***,a membrane-permeable peptide was used to block the ubiquitination of K1082 on GluN2B in AD *** circRIMS2 was significantly upregulated in 4-month-old APP/PS1 mice,which was mediated by METTL3-dependent N6-methyladenosine(m6A)*** of circRIMS2 led to synaptic and memory impairments in 4-month-old C57BL/6 ***-3968/UBE2K was validated as the downstream of *** UBE2K induced synaptic dysfunction of AD through ubiquitinating K1082 on *** METTL3 or blocking the ubiquitination of K1082 on GluN2B with a short membrane-permeable peptide remarkably rescued synaptic dysfunction in AD *** In conclusion,our study demonstrated that m6A-modified circRIMS2 mediates the synaptic and memory impairments in AD by activating the UBE2K-dependent ubiquitination and degradation of GluN2B via sponging miR-3968,providing novel therapeutic strategies for AD.

关键词： Alzheimer’s disease circRNA Synaptic dysfunction GluN2B Ubiquitination

A novel transgenic mouse line with hippocampus-dominant and inducible expression of truncated human tau

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Translational Neurodegeneration 2023年第1期12卷 154-169页

作者： Yang Gao Yuying Wang Huiyang Lei Zhendong Xu Shihong Li Haitao Yu jiazhao xie Zhentao Zhang Gongping Liu Yao Zhang Jie Zheng Jian-Zhi Wang Department of Pathophysiology Key Laboratory of Ministry of Education for Neurological DisordersSchool of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Neurology Renmin Hospital of Wuhan UniversityWuhan 430030China Key Laboratory of Ministry of Education for Neurological Disorders Department of EndocrineLiyuan HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430077China Neuroscience Research Institute and Department of Neurobiology School of Basic Medical SciencesPeking UniversityBeijingChina Key Laboratory for Neuroscience Ministry of Education/National Health CommissionPeking UniversityBeijing 100083China Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226000China

Background Intraneuronal accumulation of hyperphosphorylated tau is a defining hallmark of Alzheimer’s disease(AD).However,mouse models imitating AD-exclusive neuronal tau pathologies are *** We generated a new tet-on transgenic mouse model expressing truncated human tau N1-368(termed hTau368),a tau fragment increased in the brains of AD patients and aged mouse ***(dox)was administered in drinking water to induce hTau368 *** and Western blotting were performed to measure the tau *** sequencing was performed to evaluate gene expression,and several behavioral tests were conducted to evaluate mouse cognitive functions,emotion and *** Dox treatment for 1-2 months at a young age induced overt and reversible human tau accumulation in the brains of hTau368 transgenic mice,predominantly in the ***,the transgenic mice exhibited AD-like high level of tau phosphorylation,glial activation,loss of mature neurons,impaired hippocampal neurogenesis,synaptic degeneration and cognitive *** This study developed a well-characterized and easy-to-use tool for the investigations and drug development for AD and other tauopathies.

关键词： Alzheimer’s disease Animal model Tau hyperphosphorylation Truncated tau Tet-on system

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对我国浮选机的分析及其研究的探讨

江西理工大学学报 1985年第Z1期 84-92页

作者：谢家剑

本文对我国目前使用的和试用的几种主要浮选机作了设计理论基础的陈述,结合生产实践进行了优缺点的分析;并对我国浮选机的研究现状、研究方法、测试手段和研究成果等方面进行了分析和探讨。

关键词：浮选机主要优点射式 CHF 矿浆选矿机械叶轮转速选别指标

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JZF浮选机工作原理

南方冶金学院学报 1989年第1期10卷 33-39页

作者：谢家钊南方冶金学院矿业系

本文利用流体动力学的原理,结合试验研究,对JZF浮选机的槽体结构、叶轮结构、吸气原理,搅拌原理和矿浆运动轨迹作了初步分析,从理论上证实了该机结构的合理性和工作原理的先进性.

关键词：浮选机流体动力学矿浆

细胞程序性死亡因子10调控Notch1信号介导胶质瘤细胞的迁移

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中华实验外科杂志 2023年第10期40卷 2026-2029页

作者：万学焱谢家钊漆艺玮吴思思鄢子盛赵恺孔繁丽梅齐张所军舒凯雷霆华中科技大学同济医学院附属同济医院神经外科武汉430030 华中科技大学同济医学院基础医学院病理生理学系武汉430030 武汉儿童医院麻醉科武汉430019 华中科技大学同济医学院附属同济医院肿瘤中心武汉430030

目的探讨程序性细胞因子10(PDCD10)介导胶质瘤迁移的分子机制。方法采取慢病毒感染法构建沉默PDCD10的U251胶质瘤细胞系,同时采用Notch信号通路抑制剂DAPT处理细胞,观察沉默PDCD10细胞对胶质瘤细胞行为的影响;采用免疫荧光染色、实时定... 详细信息

目的探讨程序性细胞因子10(PDCD10)介导胶质瘤迁移的分子机制。方法采取慢病毒感染法构建沉默PDCD10的U251胶质瘤细胞系,同时采用Notch信号通路抑制剂DAPT处理细胞,观察沉默PDCD10细胞对胶质瘤细胞行为的影响;采用免疫荧光染色、实时定量聚合酶链反应和蛋白免疫印迹法分别检测胶质瘤细胞系对照组和沉默组PDCD10和Notch1的表达,探讨PDCD10调控Notch1蛋白水平的机制。两组间比较采用t检验,多组间均数比较采用多因素方差分析。结果免疫荧光染色结果显示,胶质瘤细胞的PDCD10表达水平随着胶质瘤级别的提高而降低,划痕试验和Transwell实验表明沉默PDCD10促进肿瘤细胞的迁移[(100±28)%比(152±25)%比(113±9)%、(100±3)%比(115±2)%比(99±2)%,F=7.15、88.30,P0.05],但抑制Notch1蛋白的降解速率(t=3.22,P<0.05),导致细胞内Notch1蛋白水平提高[(100±12)%比(153±16)%,F=10.05,P<0.05],进而促进胶质瘤细胞的迁移。结论PDCD10可能通过调控Notch1在细胞内的降解进而介导胶质瘤细胞的迁移。

关键词：胶质瘤程序性细胞因子10 Notch1信号通路迁移

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多层复合材料屏蔽电磁波的理论模型

四川联合大学学报（工程科学版） 1998年第6期2卷 114-118页

作者：毛健黄婉霞陈家钊吴行谢宁涂铭旌四川联合大学材料科学与工程学院

本文从基本的电磁波传播理论出发，考虑了电磁波在屏蔽层中的反射、衰减及多次反射，提出了多层复合材料屏蔽效能（ＳｈｉｅｌｄｉｎｇＥｆｅｃｔｉｖｅｎｅｓ，简称ＳＥ）的理论模型和计算公式。公式的应用简便、快捷、适用范围广，可... 详细信息

本文从基本的电磁波传播理论出发，考虑了电磁波在屏蔽层中的反射、衰减及多次反射，提出了多层复合材料屏蔽效能（ＳｈｉｅｌｄｉｎｇＥｆｅｃｔｉｖｅｎｅｓ，简称ＳＥ）的理论模型和计算公式。公式的应用简便、快捷、适用范围广，可用于不同入射方向的电磁波入射多层复合材料的屏蔽效能计算及预测。最后对公式进行了验证，计算结果与实验数据基本吻合。

关键词：多层复合材料电磁波屏蔽材料屏蔽效能

Deep Segmentation Feature-Based Radiomics Improves Recurrence Prediction of Hepatocellular Carcinoma

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Biomedical Engineering Frontiers 2022年第1期3卷 126-137页

作者： Jifei Wang Dasheng Wu Meili Sun Zhenpeng Peng Yingyu Lin Hongxin Lin jiazhao Chen Tingyu Long Zi-Ping Li Chuanmiao xie Bingsheng Huang Shi-Ting Feng Department of Radiology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Medical AI Lab School of Biomedical EngineeringHealth Science CentreShenzhen UniversityShenzhenChina Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions ShenzhenChina Department of Medical Imaging and Interventional Radiology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouChina

Objective and Impact *** study developed and validated a deep semantic segmentation feature-based radiomics(DSFR)model based on preoperative contrast-enhanced computed tomography(CECT)combined with clinical information to predict early recurrence(ER)of single hepatocellular carcinoma(HCC)after curative *** prediction is of great significance to the therapeutic decision-making and surveillance strategy of *** prediction is important for ***,it cannot currently be adequately ***,208 patients with single HCC after curative resection were retrospectively recruited into a model-development cohort(n=180)and an independent validation cohort(n=28).DSFR models based on different CT phases were *** optimal DSFR model was incorporated with clinical information to establish a DSFR-C *** integrated nomogram based on the Cox regression was *** DSFR signature was used to stratify high-and low-risk ER ***.A portal phase-based DSFR model was selected as the optimal model(area under receiver operating characteristic curve(AUC):development cohort,0.740;validation cohort,0.717).The DSFR-C model achieved AUCs of 0.782 and 0.744 in the development and validation cohorts,*** the development and validation cohorts,the integrated nomogram achieved C-index of 0.748 and 0.741 and time-dependent AUCs of 0.823 and 0.822,respectively,for recurrence-free survival(RFS)*** RFS difference between the risk groups was statistically significant(P<0.0001 and P=0.045 in the development and validation cohorts,respectively).***-based DSFR can predict ER in single HCC after curative resection,and its combination with clinical information further improved the performance for ER prediction.

关键词： Carcinoma signature Deep

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对地观测卫星任务规划策略及应用研究综述

无线电工程 2021年第8期51卷 681-690页

作者：许宇栋周敬博尹嘉昭谢剑锋武炳阳北京市遥感信息研究所北京100192

针对观测卫星任务规划过程中的4个关键环节,分析总结具有代表性的研究成果和结论。分析了观测任务预处理技术,包括需求分析、区域目标分解和点目标聚类;对任务规划的模型构建和算法优化方法进行梳理,对多星协同、快速响应和星上自主3种... 详细信息

针对观测卫星任务规划过程中的4个关键环节,分析总结具有代表性的研究成果和结论。分析了观测任务预处理技术,包括需求分析、区域目标分解和点目标聚类;对任务规划的模型构建和算法优化方法进行梳理,对多星协同、快速响应和星上自主3种规划方法作了重点论述。研究了规划策略的实际应用情况,对规划策略效能评估体系归类评述。

关键词：对地观测遥感卫星观测任务任务规划效能评估

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化合物半导体薄膜太阳能电池研究进展

广东化工 2017年第22期44卷 103-105页

作者：谢欣荣李京振李嘉兆广东先导稀材股份有限公司国家稀散金属工程技术研究中心广东清远511517

与第一代晶硅太阳能电池相比,柔性衬底薄膜太阳能电池具有原料消耗少、工艺简单、衬底廉价、能耗低、可弯曲、不易破碎、弱光性好、受高温影响小、易于光伏建筑一体化等特性。本文详细阐述了目前主流柔性衬底薄膜太阳能电池——化合物... 详细信息

与第一代晶硅太阳能电池相比,柔性衬底薄膜太阳能电池具有原料消耗少、工艺简单、衬底廉价、能耗低、可弯曲、不易破碎、弱光性好、受高温影响小、易于光伏建筑一体化等特性。本文详细阐述了目前主流柔性衬底薄膜太阳能电池——化合物半导体薄膜太阳能电池的基本结构、制备方法、研究进展及产业化现状,指出了存在的不足和解决的途径,并对其发展趋势和应用前景进行了展望。

关键词：薄膜太阳能电池化合物半导体