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Drosophila models used to simulate human ATP1A1 gene mutations that cause Charcot-Marie-Tooth type 2 disease and refractory seizures

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Neural Regeneration Research 2025年第1期20卷 265-276页

作者： Yao Yuan Lingqi Yu Xudong Zhuang Dongjing Wen Jin He Jingmei Hong jiayu xie Shengan Ling Xiaoyue Du Wenfeng Chen Xinrui Wang Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouFujian ProvinceChina NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate(Fujian Maternity and Child Health Hospital) FuzhouFujian ProvinceChina Medical Research Center Fujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology and Institute of Neurology of The First Affiliated Hospital Institute of Neuroscienceand Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouFujian ProvinceChina

Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory *** develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause *** located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)*** addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive *** these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut *** found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in *** heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal *** Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.

关键词： ATP1A1 Atpα bang-sensitive paralysis Charcot-Marie-Tooth disease type 2 CRISPR/Cas9 homology-directed repair Na^(+)/K^(+)-ATPase point mutation seizures sodium pump

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Discrete modulational instability and bright localized spin wave modes in easy-axis weak ferromagnetic spin chains involving the next-nearest-neighbor coupling

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Chinese Physics B 2019年第7期28卷 449-458页

作者： jiayu xie Zhihao Deng Xia Chang Bing Tang College of Physics Mechanical and Electrical Engineering Jishou University Jishou 416000 China The Collaborative Innovation Center of Manganese-Zinc-Vanadium Industrial Technology Jishou University Jishou 416000 China

We report a theoretical work on the properties of modulational instability and bright type nonlinear localized modes in one-dimensional easy-axis weak ferromagnetic spin lattices involving next-nearest-neighbor *** a linear stability analysis, we calculate the growth rates of the modulational instability, and plot the instability *** the strength of the next-nearest-neighbor coupling is large enough, two new asymmetric modulational instability regions appear near the boundary of the first Brillouin ***, analytical forms of the bright nonlinear localized modes are constructed by means of a quasi-discreteness *** influence of the next-nearest-neighbor coupling on the Brillouin zone center mode and boundary mode are *** particular, we discover a reversal phenomenon of the propagation direction of the Brillouin zone boundary mode.

关键词： modulational instability analysis intrinsic localized spin-wave modes weak ferromagnets next-nearest-neighbor couplings

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Modulational instability, quantum breathers and two-breathers in a frustrated ferromagnetic spin lattice under an external magnetic field

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Chinese Physics B 2018年第9期27卷 562-571页

作者： Wanhan Su jiayu xie Tianle Wu and Bing Tang College of Physics Mechanical and Electrical EngineeringJishou UniversityJishou 416000China

The modulational instability, quantum breathers and two-breathers in a frustrated easy-axis ferromagnetic zig-zag chain under an external magnetic field are investigated within the Hartree approximation. By means of a linear stability analysis, we analytically study the discrete modulational instability and analyze the effect of the frustration strength on the discrete modulational instability region. Using the results from the discrete modulational instability analysis, the presence conditions of those stationary bright type localized solutions are presented. On the other hand, we obtain the analytical expressions for the stationary bright localized solutions and analyze the effect of the frustration on their emergence conditions. By taking advantage of these bright type single-magnon bound wave functions obtained, quantum breather states in the present frustrated ferromagnetic zig-zag lattice are constructed. What is more, the analytical forms for quantum two-breather states are also obtained. In particular, the energy level formulas of quantum breathers and two-breathers are derived.

关键词： quantum breathers quantum two-breathers modulational instability frustrated ferromagnetic chains

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The endogenous antioxidant ability of royal jelly in Drosophila is independent of Keap1/Nrf2 by activating oxidoreductase activity

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Insect Science 2024年第2期31卷 503-523页

作者： Dongjing Wen jiayu xie Yao Yuan Lirong Shen Yufeng Yang Wenfeng Chen Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouChina School of Medicine Chongqing UniversityChongqingChina College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhouChina

Royal jelly(RJ)is a biologically active substance secreted by the hypopharyngeal and mandibular glands of worker *** is widely claimed that RJ reduces oxidative ***,the antioxidant activity of RJ has mostly been determined by in vitro chemical detection methods or by external administration drugs that cause oxidative *** RJ can clear the endogenous production of reactive oxygen species(ROS)in cells remains largely ***,we systematically investigated the antioxidant properties of RJ using several endogenous oxidative stress models of *** found that RJ enhanced sleep quality of aging Drosophila,which is decreased due to an increase of oxidative damage with *** supplementation improved survival and suppressed ROS levels in gut cells of flies upon exposure to hydrogen peroxide or to the neurotoxic agent ***,RJ supplementation moderated levels of ROS in endogenous gut cells and extended lifespan after exposure of flies to heat *** deprivation leads to accumulation of ROS in the gut cells,and RJ attenuated the consequences of oxidative stress caused by sleep loss and prolonged ***,RJ prevented cell oxidative damage caused by heat stress or sleep deprivation,with the antioxidant activity in vivo independent of Keap1/Nrf2 *** supplementation activated oxidoreductase activity in the guts of flies,suggesting its ability to inhibit endogenous oxidative stress and maintain health,possibly in humans.

关键词： antioxidant biological oxidations Drosophila oxidative stress royal jelly sleep loss

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GPCR-Gs mediates the protective effects of ginsenoside Rb1 against oxygen-glucose deprivation/re-oxygenation-induced astrocyte injury

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Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 33-43页

作者： Xi Wang Ying Liu Juan Li jiayu xie Yi Dai Minke Tang School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 102488China Taihe Hospital Hubei University of MedicineShiyan 442000China School of Medicine Hyogo Medical UniversityNishinomiya 663-8501Japan

Objectives:To investigate whether the protective actions of ginsenoside Rb1(Rb1)on astrocytes are mediated through the G_(s)-type G-protein-coupled receptor(GPCR-G_(s)).Methods:Primary astrocyte cultures derived from neonatal mouse brain were *** injury was induced via oxygen-glucose deprivation/re-oxygenation(OGD/R).Cell morphology,viability,lactate dehydrogenase(LDH)leakage,apoptosis,glutamate uptake,and brain-derived neurotrophic factor(BDNF)secretion were assessed to gauge cell survival and *** blot was used to investigate the cyclic adenosine monophosphate(cAMP)and protein kinase B(Akt)signaling ***-G_(s)-specific inhibitors and molecular docking were used to identify target ***:Rb1 at concentrations ranging from 0.8 to 5μM did not significantly affect the viability,glutamate uptake,or BDNF secretion in normal ***/R reduced astrocyte viability,increasing their LDH leakage and apoptosis *** also decreased glutamate uptake and BDNF secretion by these ***1 had protective effects of astrocytes challenged by OGD/R,by improving viability,reducing apoptosis,and enhancing glutamate uptake and BDNF ***,Rb1 activated the cAMP and Akt pathways in these *** the GPCR-G_(s) inhibitor NF449 was introduced,the protective effects of Rb1 completely disappeared,and its activation of cAMP and Akt signaling pathways was significantly ***:Rb1 protects against astrocytes from OGD/R-induced injury through GPCR-G_(s) mediation.

关键词： Ginseng Ginsenoside Rb1 Receptor GPCR Astrocytes Neuroprotective effects

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Analyses the Endowment Insurance System of Flexible Employment

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International English Education Research 2015年第6期 122-126页

作者： Rui Wang jiayu Deng Yixuan xie Wenhui road NO. 1000 songiiang district shanghai China

In modem society, flexible employment has become the important channel of improve employment. Workers who transfer into the flexible employment from regular employment easily lost the original endowment insurance rights and interests, which increase the cost of laborer to flexible employment and impede the development of flexible employment. The establishment of the endowment insurance system of flexible employment can eliminate the apprehension of supporting themselves and enjoy in old age well.

关键词： Endowment insurance Government support Flexible employment

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植物源天然产物对黄曲霉和黄曲霉毒素B_1的抑制作用研究进展

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食品与发酵工业 2021年第1期47卷 317-326页

作者：谢佳雨张雯欧杰上海海洋大学食品学院上海201306 上海水产品加工及贮藏工程技术研究中心上海201306 农业农村部水产品贮藏保鲜质量安全风险评估实验室(上海) 上海201306

黄曲霉毒素B1(aflatoxin B1,AFB1)是化学物质中致癌性最强的一种,对人和动物的健康具有极大的威胁,尤其是对肝脏的损害。AFB1是谷物和粮油中最为多见的真菌毒素,危害性也最强,长期低剂量的摄入AFB1除了会导致肝脏损伤和诱发肝癌之外,也... 详细信息

黄曲霉毒素B1(aflatoxin B1,AFB1)是化学物质中致癌性最强的一种,对人和动物的健康具有极大的威胁,尤其是对肝脏的损害。AFB1是谷物和粮油中最为多见的真菌毒素,危害性也最强,长期低剂量的摄入AFB1除了会导致肝脏损伤和诱发肝癌之外,也会损害人体的其他器官。因此,能够有效防护人体免遭AFB1损伤的功能食品和新型保健药物已成为研究重点。近年来,天然产物因其具有生物活性显著、副作用小、多靶点等优点而备受关注,多种植物源天然产物已被报道能够抑制黄曲霉的生长产毒和AFB1的毒性作用。该文主要综述了植物源天然产物对黄曲霉生长产毒和AFB1毒性作用的抑制作用以及其相关抑制机制,为进一步开展AFB1毒性损伤的防护研究工作,开发能降低AFB1对人和动物的毒性损伤的新型保健药物和功能食品提供参考。

关键词：天然产物黄曲霉黄曲霉毒素B_1 生物活性抑制机制

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大采高工作面矿压规律自动分析与仿真研究

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煤炭科学技术 2021年第4期49卷 186-192页

作者：王超谢佳玉徐放艳孔超兖州煤业股份有限公司山东邹城273500 山东科技大学能源与矿业工程学院山东青岛266590 山东思科赛德矿业安全工程有限公司山东泰安271000 山东爱拓软件开发有限公司山东泰安271000

为了高效、科学、经济地对采场顶板运动趋势以及变化情况进行有效分析,掌握其活动规律,借鉴工业生产中"计划-实施-检查-处置"(PDCA)的循环管控思想,研发了基于PDCA循环理念的大采高工作面矿压规律自动分析与仿真系统,系统可根据工作面... 详细信息

为了高效、科学、经济地对采场顶板运动趋势以及变化情况进行有效分析,掌握其活动规律,借鉴工业生产中"计划-实施-检查-处置"(PDCA)的循环管控思想,研发了基于PDCA循环理念的大采高工作面矿压规律自动分析与仿真系统,系统可根据工作面实测矿压数据实现对采场上覆岩层力学参数的反演与修订。系统首先在工作面开采前依据模型中挠曲算法或步距算法判断传递岩梁支托层与随动层,并对相关采场覆岩运动参数进行理论预测;其次在开采中对液压支架阻力实测数据进行在线分析,提炼出顶板运动参数;最后通过反演修订采场顶板关键岩层的力学参数,使系统预测与现场实测分析结果误差逐步降低,并为采场其他相关参数的计算提供基础数据。系统在营盘壕煤矿2201工作面的应用结果表明:模型对工作面传递岩梁初次来压步距与周期来压步距等几个关键参数的计算精度得到了验证,系统仿真数据与现场实测数据的误差不断缩小,实现了对现场矿山压力显现规律进行定量分析。该研究思路对矿压显现观测理论计算与现场实测提供了融合途径。

关键词：矿压分析覆岩运动模拟预测与预报 PDCA循环

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军事信息系统项目军民融合决策评估模型

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系统工程理论与实践 2018年第10期38卷 2713-2720页

作者：孙煜飞解嘉宇苏凯李勇中国人民解放军91977部队北京102249 海军工程大学装备经济管理系武汉430033

针对军事信息系统项目军民融合事前决策评估问题,提出了一种基于改进层次分析法和云模型的军事信息系统项目军民融合需求度评估模型,为研究军民融合评估工作探索一条新的途径.利用正态分布区间数表示评估数据,并利用区间数逆向云发生器... 详细信息

针对军事信息系统项目军民融合事前决策评估问题,提出了一种基于改进层次分析法和云模型的军事信息系统项目军民融合需求度评估模型,为研究军民融合评估工作探索一条新的途径.利用正态分布区间数表示评估数据,并利用区间数逆向云发生器生成各评估指标云.同时采用兼顾形状和距离的正态云模型相似度测算方法对军民融合需求度进行综合评估.仿真实验表明,本文提出的评估模型具有很好的合理性和可用性.

关键词：军民融合决策军民融合评估云模型改进层次分析法

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综采放顶煤工作面过渡支架破网放煤装置的研究与应用

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煤矿机械 2019年第7期40卷 128-130页

作者：谢佳玉赵岩谢国龙山东科技大学矿业与安全工程学院山东青岛266590 山西潞安矿业(集团)有限责任公司山西长治046204

为有效管控综采放顶煤工作面两端头顶煤丢失导致的安全风险,提高工作面采出率,结合两端头支护和后部刮板输送机机头、机尾的要求,研制出一种用于综放工作面过渡支架顶煤回收的实用型破网装置。该装置以现有过渡支架液压系统和尾梁为基础... 详细信息

为有效管控综采放顶煤工作面两端头顶煤丢失导致的安全风险,提高工作面采出率,结合两端头支护和后部刮板输送机机头、机尾的要求,研制出一种用于综放工作面过渡支架顶煤回收的实用型破网装置。该装置以现有过渡支架液压系统和尾梁为基础,通过改造尾梁及插板,采用液压千斤顶为动力驱动插板端头刀齿机构,剪断顶网网丝。试验与应用表明,该装置一次操作可将80%~90%的金属网丝剪断形成破口,顶煤可随破口流入输送机内,调高尾梁伸出插板可减少放煤量直至关闭破口终止放煤,放煤效果良好,解决了工作面两端头过渡支架顶煤无法回收的难题,消除了顶煤丢失带来自然发火的隐患,取得了良好的经济和社会效益。

关键词：综采放顶煤工作面过渡支架顶煤破网放煤装置

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