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Frontiers in Biology 2012年第1期7卷 57-64页

作者： Yan LIU Yang SONG Ling jiang jiayu LIA Department of Bioengineering Bourns College of Engineering University of California at Riverside 900 University Avenue Riverside CA92521 USA

Forster resonance energy transfer （FRET） techniques have been widely used in biological studies in vitro andin vivo and are powerful tools for elucidating protein interactions in many regulatory cascades. FRET occurs between oscillating dipoles of two fluorophores with overlapping emission and excitation wavelengths and is dependent on the spectroscopic and geometric properties of the donor-acceptor pair. Various efforts have been made to develop quantitative FRET methods to accurately determine the interaction affinity and kinetics parameters. SUMOylation is an important post-translational protein modification with key roles in multiple biological processes. Conjugating SUMO to substrates requires an enzymatic cascade. Sentrin/SUMO-specific proteases （SENP） act as endopeptidases to process the pre-SUMO or an isopeptidase to deconjugate SUMO from its substrate. Here we also summarize recent developments of theoretical and experimental procedures for determining the protein interaction dissociation constant, Kd, and protease kinetics parameters, kcat and Kin, in the SUMOylation pathway. The general principles of these quantitative FRET-based measurements can be applied to other protein interactions and proteases.

关键词： quantitative FRET analysis protein affinity determination kinetics analysis

IGF2BP3 Enhances the Growth of Hepatocellular Carcinoma Tumors by Regulating the Properties of Macrophages and CD8^(+)T Cells in the Tumor Microenvironment

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Journal of Clinical and Translational Hepatology 2023年第6期11卷 1308-1320页

作者： Lingyu Ma jiayu jiang Qin Si Chong Chen Zhaojun Duan Department of Immunology Institute of Basic Medical SciencesChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijingChina

Background and Aims:Overexpression of IGF2BP3 is associated with the prognosis of hepatocellular carcinoma(HCC).However,its role in regulating tumor immune microenvironment(TME)is not well ***,we investigated the effects of IGF2BP3 on macrophages and CD8^(+)T cells within the TME of ***:The relationship between IGF2BP3 and immune cell infiltration was analyzed using online bioinformatics *** of IGF2BP3 in mouse hepatoma cell line Hepa1-6 was established using CRISPR/Cas9 *** vitro cell coculture and subcutaneously implanted hepatoma mice model were used to explore the effects of IGF2BP3 on immune *** of CCL50l transforming growth factor beta 1(TGF-β1)was detected with quantitative real-time polymerase chain reaction,western blotting,and enzyme-linked immunosorbent *** binding of IGF2BP3 and its target RNA was verified by trimolecular fluorescence complementation system and RNA immunoprecipitation followed by quantitative or semiquantitative polymerase chain ***:IGF2BP3 expression was elevated in HCC and was positively correlated with macrophage *** with higher IGF2BP3 expression and lower macrophage infiltration had a better survival *** found that IGF2BP3 could bind to the mRNA of CCL5 or TGF-β1,increasing their expression,and inducing macrophage infiltration and M2 polarization while inhibiting the activation of CD8^(+)T ***,inhibition of IGF2BP3 combined with anti-CD47 antibody treatment significantly suppressed the growth of hepatoma in Hepa1-6 xenograft tu-mor ***:IGF2BP3 promoted the infiltration and M2-polarization of macrophages and suppressed CD8^(+)T activation by enhancing CCL5 and TGF-β1 expression,which facilitated the progression of Hepa1-6 xenograft tumor.

关键词： Hepatocellular carcinoma IGF2BP3 TGF-β1 CCL5 M2 macrophage CD8^(+)T cell

Targeting carnitine palmitoyl transferase 1A(CPT1A)induces ferroptosis and synergizes with immunotherapy in lung cancer

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1675-1689页

作者： Lei Ma Chong Chen Chunxing Zhao Tong Li Lingyu Ma jiayu jiang Zhaojun Duan Qin Si Tsung-Hsien Chuang Rong Xiang Yunping Luo Department of Immunology Institute of Basic Medical SciencesChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing 100005China Collaborative Innovation Center for Biotherapy Institute of Basic Medical SciencesChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing 100005China Department of Lung Cancer Surgery Tianjin Medical University General HospitalTianjin 300052China Immunology Research Center National Health Research InstitutesZhunanMiaoliTaiwanROC Department of Immunology Nankai UniversityTianjin 300071China

Despite the successful application of immune checkpoint therapy,no response or recurrence is typical in lung *** stem cells(CSCs)have been identified as a crucial player in immunotherapy-related ***,a form of cell death driven by iron-dependent lipid peroxidation,is highly regulated by cellular metabolism remolding and has been shown to have synergistic effects when combined with *** adaption of CsCs drives tumor resistance,yet the mechanisms of their ferroptosis defense in tumor immune evasion remain ***,through metabolomics,transcriptomics,a lung epithelialspecific Cptla-knockout mouse model,and clinical analysis,we demonstrate that CPT1A,a key rate-limiting enzyme of fatty acid oxidation,acts with L-carnitine,derived from tumor-associated macrophages to drive ferroptosis-resistance and CD8^(+)T cells inactivation in lung ***,CPT1A restrains ubiquitination and degradation of c-Myc,while c-Myc transcriptionally activates CPT1A *** CPT1A/c-Myc positive feedback loop further enhances the cellular antioxidant capacity by activating the NRF2/GPX4 system and reduces the amount of phospholipid polyunsaturated fatty acids through ACSL4 downregulating,thereby suppressing ferroptosis in ***,targeting CPT1A enhances immune checkpoint blockadeinduced anti-tumor immunity and tumoral ferroptosis in tumor-bearing *** results illustrate the potential of a mechanismguided therapeutic strategy by targeting a metabolic vulnerability in the ferroptosis of CsCs to improve the efficacy of lung cancer immunotherapy.

关键词： immunotherapy metabolism peroxidation

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赫梯国王名字的演变及族群认同构建

世界民族 2020年第1期 104-114页

作者：蒋家瑜首都师范大学历史学院

赫梯人所建立的赫梯文明及赫梯王国具有多元文化交融和多民族共存的特点,这在赫梯国王名字的历史演变中得以窥见。赫梯国王名字的由来包含了城市名、神灵名、高山名和祖先名等多种来源,而赫梯国王名字的由来及其历史演变同时也反映出国... 详细信息

赫梯人所建立的赫梯文明及赫梯王国具有多元文化交融和多民族共存的特点,这在赫梯国王名字的历史演变中得以窥见。赫梯国王名字的由来包含了城市名、神灵名、高山名和祖先名等多种来源,而赫梯国王名字的由来及其历史演变同时也反映出国王名字在文化上的多元性,在寓意上的积极性和时代性,以及赫梯人对王权权威性、合法性和王位正统性的追求。赫梯国王名字的演变及其文化特征是赫梯族群认同构建的必然结果。在多民族文化并存的状态下,赫梯人通过文化选择的方式逐渐形成了服务于赫梯王权的族群认同体系,这一体系在赫梯帝国灭亡之后甚至也产生了一定程度的影响。

关键词：赫梯国王名字多元文化文化选择族群认同

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赫梯宗教节日的“节中节”现象

世界历史 2019年第5期 122-134,155,156页

作者：蒋家瑜首都师范大学历史学院

赫梯的安塔赫舒节日和努恩塔瑞亚斯哈节日里存在着一种"节中节"现象,即在一个节日的举办过程中还庆祝着其它的节日。"节中节"的运行机制表现为主节日包含了副节日,但双方并行不悖、先后衔接、主次分明。"节中节"的作用在于不仅整合了赫... 详细信息

赫梯的安塔赫舒节日和努恩塔瑞亚斯哈节日里存在着一种"节中节"现象,即在一个节日的举办过程中还庆祝着其它的节日。"节中节"的运行机制表现为主节日包含了副节日,但双方并行不悖、先后衔接、主次分明。"节中节"的作用在于不仅整合了赫梯宗教节日,加速了赫梯节日的一体化,还在一定程度上维护了赫梯王国的政治统治,客观上促进了各节日及其背后不同文化间的交流与融合。"节中节"现象及其运行机制的形成是赫梯王国历史发展的必然结果,也是赫梯国王进行政治统治与文化管理的产物,因此它本质上是赫梯统治者们"有心为之"的一种文化统治手段。然而,它仅仅停留在对各个节日形式上的"嫁接",并未能形成一个起着主流作用的节日文化体系。

关键词：赫梯宗教节日安塔赫舒节日努恩塔瑞亚斯哈节日节中节

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赫梯节日空间及其国家治理策略

历史研究 2023年第2期 141-160,M0007页

作者：蒋家瑜首都师范大学历史学院北京100089

赫梯在一定空间范围内周期性举办节日庆典。赫梯国家性节日中的各类庆祝仪式,在都城和其他城市、地区辗转举办。城市与城内的神庙、宫殿等场所构成节日举办的现实空间。在节日活动中还出现想象空间,与现实空间相互交织,二者通过“巡幸... 详细信息

赫梯在一定空间范围内周期性举办节日庆典。赫梯国家性节日中的各类庆祝仪式,在都城和其他城市、地区辗转举办。城市与城内的神庙、宫殿等场所构成节日举办的现实空间。在节日活动中还出现想象空间,与现实空间相互交织,二者通过“巡幸京畿”和“四方来贺”的形式得以呈现。赫梯统治者借此扩大了节日活动的空间内涵,延展了国家宗教文化管理的空间范围,实现了掌控和威慑核心区,笼络腹地外围区、附属国以及封侯国的治理策略。

关键词：赫梯节日空间国家治理安纳托利亚文明史

Effects of Different Rootstocks on the Growth and Fruit Quality of Greenhouse Cucumber in Early Spring

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Journal of Northeast Agricultural University(English Edition) 2009年第1期16卷 10-13页

作者： LIU Bing ZHANG Tianwei ZHAO jiayu jiang Xinmei College of Horticulture Northeast Agricultural University Harbin 150030 China

Different pumpkin rootstocks were used to study the effects of different rootstocks on the growth and fruit quality of greenhouse cucumber in early spring. The results showed that the grafted cucumber could significantly improve the production, and different rootstock had a certain effect on the survival rate and fruit quality. Jinhuanghou and Fengyijiajiewang as rootstock had the highest survival rate, which were 79.0% and 70.7%, respectively. As rootstocks of greenhouse cucumber, Jinhuanghou, Lvzhoujuxing, Fengyi, Huofenghuang, and Dawei No.17 were better than others according to taste, and Heizinangua, Jinhuanghou, Fengyi, Huofenghuang, and Dawei No. 17 were better according to output.

关键词： cucumber rootstock graft quality growth

因俗制礼:赫梯节日视角下的吐塔里亚四世"宗教改革"再审视

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历史教学（下半月） 2023年第6期 21-32页

作者：蒋家瑜首都师范大学历史学院北京100089

赫梯节日是一个"因俗制礼"的长期过程,它的内容形式丰富.现存记录节日的泥板残片数量众多,文献时间跨度长,覆盖层面广,因而能够反映赫梯王国在不同历史时期的宗教文化和国家统治状况,我们可以借此来考察吐塔里亚四世"宗教改革"这一问题... 详细信息

赫梯节日是一个"因俗制礼"的长期过程,它的内容形式丰富.现存记录节日的泥板残片数量众多,文献时间跨度长,覆盖层面广,因而能够反映赫梯王国在不同历史时期的宗教文化和国家统治状况,我们可以借此来考察吐塔里亚四世"宗教改革"这一问题.从赫梯节日的角度看,吐塔里亚四世"宗教改革"是赫梯新王国晚期的"因俗制礼",它始于哈吐什里三世,经由普都海帕,直至吐塔里亚四世最终完成,本质上是赫梯统治者们为了应对赫梯新王国晚期的严峻统治形势而对宗教统治策略所采取的积极调整.

关键词：赫梯节日地方性节日赫梯王国晚期吐塔里亚四世

Rheological Behaviors and Processing Windows of Low Viscosity Epoxy Resin for VIMP

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Journal of Wuhan University of Technology(Materials Science) 2011年第5期26卷 931-934页

作者：刘卓峰 College of Aerospace and Material Engineering National University of Defense Technology

The chemorheological behaviors of a low viscosity epoxy resin system （Huntsman 1564/3486） for vacuum infusion moulding process （VIMP） were studied with viscosity *** dual-Arrhenius rheological model and the engineering viscosity model were established and compared with the experimental *** result showed that the viscosity in the earlier stage calculated by dual-Arrhenius model were smaller than the experimental data,while the data calculated by the engineering model were *** the two models together can predict the rheological behaviors of the resin system in a more credible *** processing windows of the resin system for VIMP were determined based on the two *** optimum processing temperature is 30-45 ℃.

关键词： vacuum infusion molding process(VIMP) epoxy resin rheological behaviors

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不同沥青结合料水损害的纳米尺度研究

材料研究学报 2016年第10期30卷 773-780页

作者：刘克非邓林飞郑佳宇蒋康中南林业科技大学土木工程与力学学院长沙410004 湖南省交通科学研究院长沙410015

为了从微观尺度分析不同沥青结合料的抗水损害性能,分别制备了不同状态下(干燥/潮湿、原样/老化)的70#A基质沥青、SBS改性沥青、Sasobit温拌沥青和橡胶改性沥青试样,采用原子力显微镜(AFM)分析了各试样的表面特性,测试了沥青与矿料间的... 详细信息

为了从微观尺度分析不同沥青结合料的抗水损害性能,分别制备了不同状态下(干燥/潮湿、原样/老化)的70#A基质沥青、SBS改性沥青、Sasobit温拌沥青和橡胶改性沥青试样,采用原子力显微镜(AFM)分析了各试样的表面特性,测试了沥青与矿料间的粘附力值。结果表明:干燥状态下,基质沥青与矿料间的粘附力大于改性沥青,但其测定结果的离散程度和波动性最大。潮湿状态下,原样基质沥青、Sasobit温拌沥青和橡胶改性沥青的粘附力值均有不同程度的下降,原样SBS改性沥青的对应值有较大的提高,原样SBS改性沥青具有较好的抗水损能力。沥青老化过程极大地降低基质沥青的抗水分侵蚀能力,基质沥青对水分更敏感,更易产生水损害;沥青老化也会降低SBS改性沥青的抗水损能力。

关键词：无机非金属材料沥青结合料原子力显微镜水损害微观