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Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay

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Food Quality and Safety 2022年第4期6卷 597-607页

作者： Jiaxu Xiao Hongfei Yang Liangni Qin Jixiang Liang Long Li xiaohui Fan Dapeng Peng National Reference Laboratory of Veterinary Drug Residues/Key Laboratory of Ministry of Agriculture for the Detection of Veterinary Drug Residues in Foods Huazhong Agricultural UniversityWuhanChina Wuhan Shangcheng Biotechnology Co. Ltd.WuhanChina Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhenChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Objectives:Fluoroquinolones(FQs)are widely used in aquaculture,and their residues have caused many problems threatening human health.Here,this study aims to develop a colloidal gold immunochromatographic strip based on gold-labeled microwells to screen the residues of FQs on site.Materials and methods:The Protein A Magarose Beads affinity chromatography method was adopted to purify the ascites against FQs.By using a strategy of heterologous coating antigen,different coating antigens are applied to detect FQs.The gold-labeled microwell immunochromatographic assay was used to improve the sensitivity of the test strip by the advanced reaction of antigen and antibody.Results:The antibodies were verified to be of high purity up to 99%,and the titer reached 1:1024000.The combination(enoxacin-OVA and the antibody)detected the 4 banned FQs(pefloxacin,PEF;norfloxacin,NOR;lomefloxacin,LOM;ofloxacin,OFL)with the 50%inhibiting concentration(IC50)values ranging from 1.3 to 2.1 ng/mL and cross-reactions ranging from 67.3%to 106.1%.The analysis of spiked crucian carp,silver carp,grass carp,and shrimp samples showed that the limit of detection for PEF,NOR,LOM,and OFL was 4μg/kg.A comparative study with liquid chromatography tandem mass spectrometry(LC-MS/MS)demonstrated that the assay provided an effective screening tool for the rapid detection of FQs residues.Conclusions:The results indicated that the test strip can realize full coverage recognition of the 4 banned FQs and has good accuracy,specifi-city,reproducibility,and stability;therefore,they are more suitable for rapid detection of FQs in aquatic products.

关键词： fluoroquinolones colloidal gold microwell purification residues

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Experimental study of the damage characteristics of rocks containing non-penetrating cracks under cyclic loading

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International Journal of Mining Science and Technology 2024年第2期34卷 197-210页

作者： Jun Xu xiaochun xiao Lu Ma Sen Luo jiaxu Jin Baijian Wu School of Mechanics and Engineering Liaoning Technical UniversityFuxin 123000China School of Civil Engineering Liaoning Technical UniversityFuxin 123000China Department of Engineering Mechanics Southeast UniversityNanjing 211189China

The damage evolution process of non-penetrating cracks often causes some unexpected engineering disasters.Gypsum specimens containing non-penetrating crack(s)are used to study the damage evolution and characteristics under cyclic loading.The results show that under cyclic loading,the relationship between the number of non-penetrating crack(s)and the characteristic parameters(cyclic number,peak stress,peak strain,failure stress,and failure strain)of the pre-cracked specimens can be represented by a decreasing linear function.The damage evolution equation is fitted by calibrating the accumulative plastic strain for each cycle,and the damage constitutive equation is proposed by the concept of effective stress.Additionally,non-penetrating cracks are more likely to cause uneven stress distribution,damage accumulation,and local failure of specimen.The local failure can change the stress distribution and relieve the inhibition of non-penetrating crack extension and eventually cause a dramatic destruction of the specimen.Therefore,the evolution process caused by non-penetrating cracks can be regarded as one of the important reasons for inducing rockburst.These results are expected to improve the understanding of the process of spalling formation and rockburst and can be used to analyze the stability of rocks or rock structures.

关键词： Damage characteristics Constitutive model Fissured rocks Non-penetrating crack Cyclic loading

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Realization of Oriented and Nanoporous Bismuth Chalcogenide Layers via Topochemical Heteroepitaxy for Flexible Gas Sensors

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Research 2022年第4期2022卷 211-221页

作者： Zhiwei Wang Jie Dai Jian Wang Xinzhe Li Chengjie Pei Yanlei Liu jiaxu Yan Lin Wang Shaozhou Li Hai Li xiaoshan Wang xiao Huang Wei Huang Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)30 South Puzhu RoadNanjing 211816China Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi'an 710072China Key Laboratory for Organic Electronic e Information Displays(KLOEID)and Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM)Nanjing University of Posts and Telecommunications9 Wenyuan RoadNanjing 210023China

Most van der Waals two-dimensional(2D)materials without surface dangling bonds show limited surface activities except for their edge sites.Ultrathin Bi_(2)Se_(3),a topological insulator that behaves metal-like under ambient conditions,has been overlooked on its surface activities.Herein,through a topochemical conversion process,ultrathin nanoporous Bi_(2)Se_(3) layers were epitaxially deposited on BiOCl nanosheets with strong electronic coupling,leading to hybrid electronic states with further bandgap narrowing.Such oriented nanoporous Bi_(2)Se_(3) layers possessed largely exposed active edge sites,along with improved surface roughness and film forming ability even on inkjet-printed flexible electrodes.Superior room-temperature NO_(2) sensing performance was achieved compared to other 2D materials under bent conditions.Our work demonstrates that creating nanoscale features in 2D materials through topochemical heteroepitaxy is promising to achieve both favorable electronic properties and surface activity toward practical applications.

关键词： chemical bonds roughness

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Assessment on the Diagnostic Capacity for Parasitic Diseases of Health Facilities—China,2019

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China CDC weekly 2021年第35期3卷 731-735页

作者： Qiang Wang Jing Xu Yuwan Hao Tian Tian Zelin Zhu Xueqiang Zhang Liying Wang Shaohong Chen jiaxu Chen Ning xiao Shizhu Li National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesShanghaiChina.

What is already known about this topic?Morbidity and prevalence of several major parasitic diseases have been declining in China.To reduce the disease burden of parasitic diseases and protect public health security,conducting accurate diagnoses following timely treatment is important.What is added by this report?In the national competition held in 2019.

关键词： China. conducting burden

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自适应深层残差3D-CNN高光谱图像快速分类算法

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计算机辅助设计与图形学学报 2019年第11期31卷 2017-2029页

作者：肖志云蒋家旭倪晨内蒙古工业大学电力学院呼和浩特010080 内蒙古自治区机电控制重点实验室呼和浩特010080

为了实现高光谱图像的快速训练、分类和超参数自适应寻优,提出基于深层残差3D卷积神经网络(3D-CNN)的高光谱图像识别分类算法.由于采用的3D特征提取算法更适合高光谱3D数据结构,使得网络可以快速地从完整的高光谱图像样本中同时提取丰... 详细信息

为了实现高光谱图像的快速训练、分类和超参数自适应寻优,提出基于深层残差3D卷积神经网络(3D-CNN)的高光谱图像识别分类算法.由于采用的3D特征提取算法更适合高光谱3D数据结构,使得网络可以快速地从完整的高光谱图像样本中同时提取丰富的空间和光谱特征;此外,通过对高光谱图像样本平面空间方向的旋转和翻转操作进行数据增强的方法;以及运用TPE超参数优化算法对设定的超参数选择空间自适应寻优的方法,都可以有效地提高分类准确率.通过在TensorFlow框架下对Pavia University, Indian Pines和KSC等标准高光谱数据集上的实验结果表明,与SSRN等其他算法相比,文中算法在加深网络结构的同时,提高了分类准确率;与人工设定超参数网络相比,以TPE自适应超参数优化算法优化的网络参数数量减少约一半,训练时间缩短约10%.

关键词：残差网络 3D卷积神经网络自适应超参数优化高光谱图像分类

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Atomically precise metal chalcogenide supertetrahedral clusters:frameworks to molecules,and structure to function

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National Science Review 2022年第1期9卷 128-143页

作者： jiaxu Zhang Pingyun Feng Xianhui Bu Tao Wu College of Chemistry Chemical Engineering and Material Science Soochow University Department of Chemistry University of California College of Chemistry and Materials Science Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications Jinan University Department of Chemistry and Biochemistry California State University

Metal chalcogenide supertetrahedral clusters(MCSCs) are of significance for developing crystalline porous framework materials and atomically precise cluster chemistry.Early research interest focused on the synthetic and structural chemistry of MCSC-based porous semiconductor materials with different cluster sizes/compositions and their applications in adsorption-based separation and optoelectronics.More recently,focus has shifted to the cluster chemistry of MCSCs to establish atomically precise structure-composition-property relationships,which are critical for regulating the properties and expanding the applications of MCSCs.Importantly,MCSCs are similar to Ⅱ-Ⅵ or Ⅰ-Ⅲ-Ⅵ semiconductor nanocrystals(also called quantum dots,QDs) but avoid their inherent size polydispersity and structural ambiguity.Thus,discrete MCSCs,especially those that are solution-processable,could provide models for understanding various issues that cannot be easily clarified using QDs.This review covers three decades of efforts on MCSCs,including advancements in MCSC-based open frameworks(reticular chemistry),the precise structure-property relationships of MCSCs(cluster chemistry),and the functionalization and applications of MCSC-based micro crystals.An outlook on remaining problems to be solved and future trends is also presented.

关键词： metal chalcogenide nanocluster open framework semiconductor reticular chemistry atomically precise nanochemistry

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共轭参数驱动的谐波传动齿廓设计与分析方法

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机械工程学报 2022年第3期58卷 131-139页

作者：唐挺李俊阳王家序曾星宇肖科重庆大学机械与运载工程学院重庆400044 重庆大学机械传动国家重点实验室重庆400044

以谐波传动中凸轮轮廓曲线为共轭齿廓形成的原始驱动参数,基于运动轨迹映射与共轭方程,提出了共轭参数驱动的谐波传动齿廓设计方法。对非共轭参数驱动与共轭参数驱动设计方法设计的共轭齿廓进行了啮合特性对比分析,研究了凸轮径向变形... 详细信息

以谐波传动中凸轮轮廓曲线为共轭齿廓形成的原始驱动参数,基于运动轨迹映射与共轭方程,提出了共轭参数驱动的谐波传动齿廓设计方法。对非共轭参数驱动与共轭参数驱动设计方法设计的共轭齿廓进行了啮合特性对比分析,研究了凸轮径向变形量系数和凸轮形式对谐波传动啮合与接触特性的影响规律。结果表明:共轭参数驱动的齿廓设计方法无须参数调整便可同时实现二次共轭和两点共轭,并且不会出现无解;凸轮径向变形量系数的减小可增大啮合区间与啮入区的啮合侧隙,并使齿面接触的综合曲率半径减小;凸轮形式虽然对啮合区间影响较小,但是对啮合侧隙和综合曲率半径影响较大,选取凸轮形式及结构参数时应综合考虑对谐波传动啮合、接触与润滑特性的影响。

关键词：谐波传动啮合原理齿廓设计啮合特性

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少齿差行星减速器非线性耦合振动研究

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华中科技大学学报（自然科学版） 2016年第2期44卷 40-46页

作者：王家序李奇肖科石珍重庆大学机电传动与运载装备研究所重庆400030 四川大学空天科学与工程学院四川成都610065

以少齿差行星减速器为研究对象,综合考虑时变啮合刚度、齿轮传递误差、齿侧间隙及轴承支撑刚度和阻尼等因素,采用集中质量法建立了弯-扭耦合8自由度非线性振动模型,运用四阶五级的RKF法对动力学方程进行求解;研究了减速器的非线性耦合振... 详细信息

以少齿差行星减速器为研究对象,综合考虑时变啮合刚度、齿轮传递误差、齿侧间隙及轴承支撑刚度和阻尼等因素,采用集中质量法建立了弯-扭耦合8自由度非线性振动模型,运用四阶五级的RKF法对动力学方程进行求解;研究了减速器的非线性耦合振动,并进行实验分析对比.结果表明:双联齿轮振动位移和速度最小,固定齿轮扭转角位移和角速度最小,且扭转方向比y方向的振动平缓;固定齿轮、输出齿轮振动为近混沌状态,双联齿轮振动复杂为混沌运动状态;齿轮动态啮合力和轴承动载荷均呈现周期性.该耦合动力学模型仿真结果与实验测试结果基本符合.

关键词：行星减速器集中质量法非线性振动齿轮振动特性混沌运动

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The First Reported Case of COVID-19 and Plasmodium ovale Malaria Coinfection—Guangdong Province,China,January 2021

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China CDC weekly 2021年第21期3卷 454-455页

作者： Muxin Chen Shitong Gao Lin Ai jiaxu Chen Tiejian Feng Zhigao Chen xiaomin Zhang Shaoyu Deng Zidan Lin Yijun Tang Qian Zhang Gengcheng He Huawei Xiong xiao-nong Zhou Renli Zhang Dana Huang Institute of pathogenic biology Shenzhen Center for Disease Control and PreventionShenzhenGuangdongChina National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector Biology(National Institute of Parasitic DiseasesChinese Center for Disease Control and Prevention)National Center for International Research on Tropical DiseasesMinistry of Science and TechnologyWHO Collaborating Center for Tropical DiseasesShanghaiChina Department of Communicable Diseases Control and Prevention Shenzhen Center for Disease Control and PreventionShenzhenGuangdongChina National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention-Shenzhen Center for Disease Control and Prevention Joint Laboratory for Imported Tropical Disease ControlShanghaiChina Shenzhen Center for Disease Control and Prevention ShenzhenGuangdongChina.

The current coronavirus disease 2019(COVID-19)pandemic has posed an unprecedented challenge to global health(1–3).The clinical symptoms of COVID-19 are similar to those of malaria,such as fever,myalgia,fatigue,headache,and gastrointestinal symptoms.Due to the heavy burden of medical services and the possible shortage of resources caused by the long-term COVID-19 pandemic,the risk of coinfection of malaria and COVID-19 is a matter of particular concern.Here we reported the first case of COVID-19 coinfection with Plasmodium ovale(P.ovale)malaria in a 47-year-old man who had been previously diagnosed with Plasmodium infection and incompletely treated with antimalarial drugs.

关键词： incomplete COV services

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Single-atom catalysts for neutral zinc-air batteries

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Science China Materials 2024年第5期67卷 1674-1675页

作者： jiaxu Zhang Minghao Yu Faculty of Chemistry and Food Chemistry & Center for Advancing Electronics Dresden(CfAED) Technische Universit?t Dresden

The escalating global energy demand underscores the urgency of developing sustainable battery technologies. Zinc-air batteries(ZABs) have emerged as promising candidates due to their costeffectiveness, safety, and high energy density [1–4]. Conventional ZABs typically rely on a four-electron(4e-) oxygen reduction reaction(ORR) in strongly alkaline electrolytes,

关键词： battery neutral typically

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