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The genome of Medicago polymorpha provides insights into its edibility and nutritional value as a vegetable and forage legume

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Horticulture Research 2021年第1期8卷 565-576页

作者： jiawen cui Zhaogeng Lu Tianyi Wang Gang Chen Salma Mostafa Hailong Ren Sian Liu Chunxiang Fu Li Wang Yingfang Zhu Jinkai Lu Xiang Chen Zhenwu Wei Biao Jin College of Horticulture and Plant Protection Yangzhou University225009 YangzhouChina College of Animal Science and Technology Yangzhou University225009 YangzhouChina Novogene Bioinformatics Institute 100083 BeijingChina College of Bioscience and Biotechnology Yangzhou University225009 YangzhouChina Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences266101 QingdaoChina State Key Laboratory of Crop Stress Adaptation and Improvement Key Laboratory of Cotton BiologySchool of Life SciencesHenan University475001 KaifengChina

Medicago polymorpha is a nutritious and palatable forage and vegetable plant that also fixes ***,we reveal the chromosome-scale genome sequence of *** using an integrated approach including Illumina,PacBio and Hi-C *** combined PacBio full-length RNA-seq,metabolomic analysis,structural anatomy analysis and related physiological indexes to elucidate the important agronomic traits of *** for forage and vegetable *** assembled *** genome consisted of 457.53Mb with a long scaffold N50 of 57.72Mb,and 92.92%(441.83Mb)of the assembly was assigned to seven *** genomic analysis revealed that expansion and contraction of the photosynthesis and lignin biosynthetic gene families,respectively,led to enhancement of nutritious compounds and reduced lignin biosynthesis in *** addition,we found that several positively selected nitrogen metabolism-related genes were responsible for crude protein ***,the metabolomic results revealed that a large number of flavonoids,vitamins,alkaloids,and terpenoids were enriched in *** results imply that the decreased lignin content but relatively high nutrient content of *** enhance its edibility and nutritional value as a forage and *** genomic data provide a genetic basis that will accelerate functional genomic and breeding research on *** as well as other Medicago and legume plants.

关键词： forage breeding value

The Jasmine(Jasminum sambac)Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates

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Genomics, Proteomics & Bioinformatics 2023年第1期21卷 127-149页

作者： Gang Chen Salma Mostafa Zhaogeng Lu Ran Du jiawen cui Yun Wang Qinggang Liao Jinkai Lu Xinyu Mao Bang Chang Quan Gan Li Wang Zhichao Jia Xiulian Yang Yingfang Zhu Jianbin Yan Biao Jin College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China College of Bioscience and Biotechnology Yangzhou UniversityYangzhou 225009China Department of Floriculture Faculty of AgricultureAlexandria UniversityAlexandria 21526Egypt Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou UniversityYangzhou 225009China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China College of Landscape Architecture Nanjing Forestry UniversityNanjing 210037China Institute of Plant Stress Biology State Key Laboratory of Cotton BiologyDepartment of BiologyHenan UniversityKaifeng 475001China

Jasminum sambac(jasmine flower),a world-renowned plant appreciated for its exceptional flower fragrance,is of cultural and economic ***,the genetic basis of its fragrance is largely ***,we present the first de novo genome assembly of *** with 550.12 Mb(scaffold N50=40.10 Mb)assembled into 13 *** synthase(TPS)genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the *** clusters within the salicylic acid/benzoic acid/theobromine(SABATH)and benzylalcohol O-acetyltransferase/anthocyanin O-hydroxycinnamoyltransferases/anthranilate N-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-O-acetyltransferase(BAHD)superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid *** key genes involved in jasmonate biosynthesis were duplicated,causing an increase in copy *** addition,multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis ***,the roles of JsTPS3 in b-ocimene biosynthesis,as well as JsAOC1 and JsAOS in jasmonic acid biosynthesis,were functionally *** genome assembled in this study for *** offers a basic genetic resource for studying floral scent and jasmonate biosynthesis,and provides a foundation for functional genomic research and variety improvements in Jasminum.

关键词： Jasmine flower Jasminum sambac Genome Jasmonate Flower fragrance

Genome-wide identification of circular RNAs in Arabidopsis thaliana across the lifespan

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Genome-wide identification of circular RNAs in Arabidopsis t...

2016年全国植物生物学大会

作者： Gang Chen jiawen cui Li Wang Zhaogen Lu Biao Jin College of Bioscience and Biotechnology Yangzhou University College of Horticulture and Plant Protection Yangzhou University

Circular RNAs(circ RNAs) are a newly identified class of non-coding RNA with the presence of a covalent bond linking the 3′ and 5′ ends generated by backsplicing. Large amount of circ RNAs has been identified in humans,mammals,fish,insects,worms,fungi,and protists,whereas little is known about plant circ RNAs especially in tissue/developmental-stage-specific expression. Here,we conducted a genome-wide identification of circ RNAs in Arabidopsis thaliana across lifespan and presented the profile of complete circ RNAs via deep sequencing of RNA samples with r RNA and other linear RNA removed. In total,1217 circ RNAs were identified from the mixed samples which covered seven developmental stages in A. thaliana,using the strict threshold of at least two unique backspliced supporting reads. The identified circ RNAs showed a non-random distribution in all of the chromosomes,and most were exonic circ RNAs or located within a transcript. Functional annotation analysis indicated that many circ RNAs were related to photosynthesis. In addition,large proportions of circ RNAs were potential miRNA targets,and almost half of the identified circ RNAs had more than two different miRNA-binding sites,which possibly affecting the post-transcriptional regulation of genes by acting as miRNA sponges. Overall,our data provide an important resource for the emerging circ RNA research in A. thaliana and should contribute to enhanced understanding of circ RNAs in plants.

关键词： Circular RNA Arabidopsis deep sequence back-splice

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天宫二号数据大气细颗粒物遥感反演

遥感信息 2023年第5期38卷 122-129页

作者：李芮陈健崔嘉文南京信息工程大学遥感与测绘工程学院南京210044

针对天宫二号(TG-2)大气细颗粒物遥感反演及可行性问题,利用TG-2宽波段成像仪的可见近红外波段数据,结合MOD09A1地表反射率产品构建地表反射率库,使用6S传输模型和深蓝算法反演气溶胶光学厚度(aerosol optical depth,AOD),并结合能见度... 详细信息

针对天宫二号(TG-2)大气细颗粒物遥感反演及可行性问题,利用TG-2宽波段成像仪的可见近红外波段数据,结合MOD09A1地表反射率产品构建地表反射率库,使用6S传输模型和深蓝算法反演气溶胶光学厚度(aerosol optical depth,AOD),并结合能见度和相对湿度数据对AOD进行订正,利用统计模型估算了鲁豫皖典型地区的大气细颗粒物PM_(2.5)浓度,并对PM_(2.5)浓度的空间分布进行分析。结果表明:天宫二号数据在AOD和PM_(2.5)遥感反演中具有较高的可行性,拟合度分别达到0.910和0.902;经过垂直订正和湿度订正,PM_(2.5)和AOD之间的相关性大幅度提高;PM_(2.5)的空间分布呈“污染岛”特征,这在气溶胶排放和扩散研究中起着重要作用。天宫二号PM_(2.5)反演为区域大气污染监测提供了一种有效手段。

关键词：气溶胶光学厚度天宫二号深蓝算法地表反射率 PM_(2.5) 垂直订正湿度订正

基于天宫二号宽波段成像仪数据的气溶胶光学厚度遥感反演研究

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载人航天 2019年第6期25卷 817-824页

作者：杨越陈健崔嘉文南京信息工程大学遥感与测绘工程学院

为探究天宫二号数据用于空气溶胶光学厚度(AOD)反演的可行性,基于天宫二号宽波段成像仪数据,结合MOD09A1地表反射率产品,利用深蓝算法进行了黄河三角洲上AOD的遥感反演,并与Himawari-8 AOD产品进行了对比。结果表明:天宫二号宽波段成像... 详细信息

为探究天宫二号数据用于空气溶胶光学厚度(AOD)反演的可行性,基于天宫二号宽波段成像仪数据,结合MOD09A1地表反射率产品,利用深蓝算法进行了黄河三角洲上AOD的遥感反演,并与Himawari-8 AOD产品进行了对比。结果表明:天宫二号宽波段成像仪数据可用于AOD反演,与同时期Himawari-8产品的拟合度为0.739,两者具有一致的空间分布;天宫二号宽波段成像仪获取的数据空间分辨率较高,能反映研究区AOD空间分布细节,在气溶胶监测方面有较大的潜力。

关键词：气溶胶光学厚度天宫二号地表反射率深蓝算法

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塑料套管混凝土桩荷载-沉降特性现场试验

地下空间与工程学报 2017年第5期13卷 1194-1199,1257页

作者：鲍佳文齐昌广崔允亮陈庚宁波大学建筑工程与环境学院浙江宁波315211 浙江大学城市学院土木工程系杭州310015 河海大学岩土工程科学研究所南京210098

塑料套管混凝土桩(以下简称TC桩)是由预先打设在地基中的塑料套管内浇注混凝土组成的。以TC桩软基处理的交通运输工程为依托,对TC桩单桩和复合地基进行了静载试验以了解TC桩的荷载-沉降特性。试验结果分析表明:TC桩单桩和复合地基的荷载... 详细信息

塑料套管混凝土桩(以下简称TC桩)是由预先打设在地基中的塑料套管内浇注混凝土组成的。以TC桩软基处理的交通运输工程为依托,对TC桩单桩和复合地基进行了静载试验以了解TC桩的荷载-沉降特性。试验结果分析表明:TC桩单桩和复合地基的荷载-沉降曲线中无明显陡降且沉降量较低,现有规范难以确定其极限承载力,故引入了5种单桩极限承载力的经验判定方法和复合地基极限承载力经典计算公式,并提出了3条TC桩单桩极限承载力的基本判定原则,表明在实测荷载-沉降曲线的基础上,Chin法较适合判定TC桩单桩和复合地基的极限承载力。

关键词：塑料套管混凝土桩现场试验极限承载力荷载-沉降曲线复合地基

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初中英语以“读”促“写”教学的问题与对策

英语教师 2022年第12期22卷 67-69页

作者：崔嘉雯广东省佛山市南海区西樵镇樵北中学广东佛山528200

针对初中英语以“读”促“写”教学存在的问题,从教学设计、策略、思维等三个方面提出对策。认为教师在开展以“读”促“写”教学时,要精心设计,引导学生在充分掌握基础语言知识、技能的基础上,学会从语篇角度分析作者的写作意图,文章... 详细信息

针对初中英语以“读”促“写”教学存在的问题,从教学设计、策略、思维等三个方面提出对策。认为教师在开展以“读”促“写”教学时,要精心设计,引导学生在充分掌握基础语言知识、技能的基础上,学会从语篇角度分析作者的写作意图,文章的文化内涵、写作技巧;注重策略培养,引导学生养成通过阅读积累知识的习惯,提升运用所学英语知识写作的兴趣和积极性;注重思维能力培养,使学生形成多角度、深度解读文本的能力,促使其高质量地完成写作,从而实现以“读”促“写”,提升综合语言运用能力。

关键词：以“读”促“写” 初中英语读写结合

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中泰课堂文化差异分析及对策研究

文化创新比较研究 2022年第3期6卷 157-160页

作者：崔佳文河南理工大学文法学院河南焦作454000

课堂文化是实践活动的产物,是师生长期在课堂教学实践中协作而产生的价值理念和行为规范。中国和泰国的课堂有很大的区别,这些区别具体表现为教育理念、时间理念、学生的课堂秩序等方面。这些截然不同的课堂文化反映了两国人民不同的价... 详细信息

课堂文化是实践活动的产物,是师生长期在课堂教学实践中协作而产生的价值理念和行为规范。中国和泰国的课堂有很大的区别,这些区别具体表现为教育理念、时间理念、学生的课堂秩序等方面。这些截然不同的课堂文化反映了两国人民不同的价值观、思维方式和行为准则。在泰国的中国汉语教师应采取相关策略,只有积极了解和适应泰国汉语课堂文化,才可以提高对泰汉语课堂的教学效率。

关键词：课堂文化中泰对比文化差异教学建议

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电针治疗坐骨神经损伤修复机制的研究进展

中国医药导报 2023年第17期20卷 53-56页

作者：王杰崔佳雯上海中医药大学康复医学院上海201203

关于神经损伤的治疗一直是临床上的难点问题之一。电针作为一种传统中医与现代医学相结合的治疗手法,被广泛用于治疗坐骨神经损伤,但其机制尚不完全清楚。因此,本文通过查阅近年来关于电针治疗坐骨神经损伤的文献研究,介绍了电针对于坐... 详细信息

关于神经损伤的治疗一直是临床上的难点问题之一。电针作为一种传统中医与现代医学相结合的治疗手法,被广泛用于治疗坐骨神经损伤,但其机制尚不完全清楚。因此,本文通过查阅近年来关于电针治疗坐骨神经损伤的文献研究,介绍了电针对于坐骨神经损伤后疼痛和运动功能的疗效,并从电针促进施万细胞增殖、抑制炎症反应、促进神经营养因子分泌、抑制神经元凋亡4个方面归纳电针可能的治疗机制,为临床推广应用和基础研究提供参考和依据。

关键词：坐骨神经损伤电针神经修复机制