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PECTATE LYASE-LIKE10 is associated with pollen wall development in Brassica campestris

Journal of Integrative Plant Biology 2014年第11期56卷 1095-1105页

作者： Jingjing Jiang Lina Yao Youjian Yu Meiling Lv Ying Miao jiashu cao Laboratory of Cell and Molecular Biology Institute of Vegetable ScienceZhejiang University State Key Laboratory of Agrobiotechnology Shenzhen Base Shenzhen Research InstituteThe Chinese University of Hong Kong The Center of Molecular Cell and Systems Biology College of Life ScienceFujian Agriculture and Forestry University

PECTATE LYASE‐LIKE10（PLL10） was previously identified as one of the differentially expressed genes both in microspores during the late pollen developmental stages and in pistils during the fertilization process in Chinese cabbage（Brassica campestris ssp. chinensis）. Here, antisense‐RNA was used to study the functions of BcPLL10 in Chinese cabbage. Abnormal pollen was identified in the transgenic lines（bcpll10‐4, ‐5, and ‐6）. In fertilization experiments, fewer seeds were harvested when the antisense‐RNA lines were used as pollen donor. In vivo and in vitro pollen germination assays less germinated pollen tubes were observed in bcpll10 lines. Scanning electron microscopy observation verified that the tryphine materials were over accumulated around the pollen surface and sticked them together in ***, transmission electron microscopy observation revealed that the internal endintine was overdeveloped and predominantly occupied the intine, and disturbed thenormal proportional distribution of the two layers in the non‐germinal furrow region; and no obvious demarcation existed between them in the germinal furrow region in the bcpll10 pollen. Collectively, this study presented a novel PLL gene that played an important role during the pollen wall development in B. campestris, which may also possess potential importance for male sterility usage in agriculture.

关键词： Brassica campestris Brassica rapa Chinese cabbage exine intine pectate lyase PLL pollen wall

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WRKY transcription factors in plant responses to stresses

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Journal of Integrative Plant Biology 2017年第2期59卷 86-101页

作者： Jingjing Jiang Shenghui Ma Nenghui Ye Ming Jiang jiashu cao Jianhua Zhang State Key Laboratory of Agrobiotechnology Shenzhen Base Shenzhen Research Institute The Chinese University of Hong Kong Shenzhen 518057 China Laboratory of Cell ＆ Molecular Biology Institute of Vegetable Science Zhejiang University Hangzhou 310058 China Ecology Key Discipline of Zhejiang Province College of Life Science Taizhou University Jiaojiang 318000 China School of Life Sciences and State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong Hong Kong China

The WRKY gene family is among the largest families of transcription factors （TFs） in higher plants. By regulating the plant hormone signal transduction pathway, these TFs play critical roles in some plant processes in response to biotic and abiotic stress, Various bodies of research have demonstrated the important biological functions of WRKY TFs in plant response to different kinds of biotic and abiotic stresses and working mecha- nisms. However, very li2ttle summarization has been done to review their research progress. Not iust important TFs function in plant response to biotic and abiotic stresses, WRKY also participates in carbohydrate synthesis, senes- cence, development, and secondary metabolites synthesis. WRKY proteins can bind to W-box （TGACC （A/T）） in the promoter of its target genes and activate or repress the expression of downstream genes to regulate their stress response. Moreover, WRKY proteins can interact with other TFs to regulate plant defensive responses. In the present review, we focus on the structural characteristics of WRKY TFs and the research progress on their functions in plant responses to a variety of stresses.

关键词： WRKY transcription factors in plant responses to stresses

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Polyamine Accumulation in Transgenic Tomato Enhances the Tolerance to High Temperature Stress

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Journal of Integrative Plant Biology 2009年第5期51卷 489-499页

作者： Lin Cheng Yijing Zou Shuli Ding Jiajing Zhang Xiaolin Yu jiashu cao Gang Lu Key Laboratory of Horticultural Plant Growth Development and Biotechnology Ministry of Agriculture Department of Horticulture Zhejiang University Hangzhou 310029 China

Polyamines play an important role in plant response to abiotic stress. S-adenosyl-l-methionine decarboxylase （SAMDC） is one of the key regulatory enzymes in the biosynthesis of polyamines. In order to better understand the effect of regulation of polyamine biosynthesis on the tolerance of high-temperature stress in tomato, SAMDC cDNA isolated from Saccharomyces cerevisiae was introduced into tomato genome by means of Agrobacterium tumefaciens through leaf disc transformation. Transgene and expression was confirmed by Southern and Northern blot analyses, respectively. Transgenic plants expressing yeast SAMDC produced 1.7- to 2.4-fold higher levels of spermidine and spermine than wildtype plants under high temperature stress, and enhanced antioxidant enzyme activity and the protection of membrane lipid peroxidation was also observed. This subsequently improved the efficiency of CO2 assimilation and protected the plants from high temperature stress, which indicated that the transgenic tomato presented an enhanced tolerance to high temperature stress （38℃） compared with wild-type plants. Our results demonstrated clearly that increasing polyamine biosynthesis in plants may be a means of creating high temperature-tolerant germplasm.

关键词： heat stress Lycopersicon esculentum polyamines Saccharomyces cerevisiae S-adenosylmethionine decarboxylase transformation.

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ELECTRODE SELECTION OF ELECTROLYSIS WITH MEMBRANE FOR SODIUM TUNGSTATE SOLUTION

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Journal of Central South University 1999年第2期11卷 107-110页

作者： Xiao, Liansheng Wang, Wei Zhang, Qixiu Gong, Bofan cao, jiashu Department of Metallurgical Science and Engineering Central South University of Technology Changsha China

Xiao Liansheng Wang Wei Zhang Qixiu Gong Bofan cao jiashu (Department of Metallurgical Science and Engineering, Central South University of Technology, Changsha 410083, China) Abstrac By measuring the electrode polarization curves of many kinds of materials and life spans of electrodes through intensifying electrolysis in the process, the appropriate electrode materials for different stages of electrolysis of sodium tungstate solution with membrane have been selected The effects of the electrodes with different shapes on electrolysis have been investigated. The result shows that network electrode is more suitable to electrolysis of sodium tungstate solution.

关键词： sodium tungstate electrolysis membrane electrode

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TinII intron, an enhancer to affect the function of the cytoplasmic male sterility related gene T in Brassica juncea

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Science China(Life Sciences) 2013年第1期56卷 1107-1112页

作者： JIN ZhuPing WU LingLing cao jiashu CHEN ZhuJun PEI YanXi School of Life Science Shanxi University School of Chemical Engineering and Environment North University of China Institute of Vegetable Science Zhejiang University

The T gene, which was cloned from the mitochondria of tumorous stem mustard （Brassica juncea var. tumida）, is a cytoplas- mic male sterility （CMS）-related gene that can produce two transcripts, T1170 and T1243. The latter is transcribed with the un- cleaved intron Tinll. In our previous study, transgenic Arabidopsis thaliana plants over-expressing the T1243 transcript （OE-T1243） showed a severe male-sterile phenotype, whereas OE-Tll70 plants did not. However, the functional mechanism of the T gene in B. Juncea remained unknown. In this study, microscopic analyses of paraffin sections of anthers confirmed that OE-T1243 plants did not produce normal pollen, whereas OE-T1170 plants did. We analyzed the transcription of 15 anther development-related genes and found that transcript levels of nozzle/sporocyteless and barely any meristem 1 and 2 were markedly lower in OE-T1243 plants than those in wild type, while the transcript levels of these genes in OE-Tll70 plants were unchanged. To investigate the potential roles of TinH, we inserted the TinH sequence upstream of a minimal region （-60） of the cauliflower mosaic virus 35S promoter fused to the 5＇ end of the 13-glucuronidase （GUS） reporter gene. Analysis of the transgenic plants suggested that TinH acted as an enhancer to significantly increase GUS expression. The potential action mechanism is that the TinH intron acts as an enhancer to affect the function of the CMS-related gene T.

关键词： cytoplasmic male sterility (CMS) alternative splicing enhancer intron

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Characterization of a novel gene, BcMF7,that is expressed preferentially in pollen of Brassica campestris L. ssp. chinensis Makino

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Science China(Life Sciences) 2007年第4期50卷 497-504页

作者： HUANG Li, cao jiashu, ZHANG YuChao & YE YiQun Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, China Laboratory of Cell & Molecular Biology Institute of Vegetable Science Zhejiang University Hangzhou China

Pollen formation is important for plant sexual reproduction. To identify the genes that are involved in pollen formation, we performed the genome-wide transcriptional profiling in the flower buds of both male meiotic cytokinesis (mmc) mutant and its wild-type plants of Brassica campestris L. ssp. chinen-sis, syn. B. rapa L. ssp. chinensis. cDNA-amplified fragment length polymorphism (cDNA-AFLP) analy-sis showed that the mmc mutation resulted in changes in expression of a variety of genes. BcMF7, a transcript-derived fragment (TDF) accumulated in the wild-type flower buds was further characterized. The BcMF7 gene has 1161 bp in length with two introns. The full-length BcMF7 cDNA has 609 bp in length and encodes a protein of 129 amino acids. The deduced amino acid sequence of BcMF7 protein shares no similarity to any function-known protein in Swiss-Prot database, but has 8 protein kinase C phosphorylation sites, 2 casein kinase II phosphorylation sites, 2 tyrosine kinase phosphorylation sites, 2 N-glycosylation sites and 2 N-myristoylation sites. Spatial and temporal expression patterns analysis showed that BcMF7 was expressed exclusively in pollen. The expression signal of BcMF7 was first detected at the tetrad stage of microspore development, reached a peak level at the uninucleate stage, and decreased to a slightly low level at the mature pollen stage. All these results show that BcMF7 may play a certain role in the signal transduction during pollen development.

关键词： Brassica campestris ssp. chinensis differentially expressed pollen development flower buds signal transduction

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Combinatorial development of antibacterial FeCoCr-Ag medium entropy alloy

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Materials Futures 2023年第2期2卷 134-141页

作者： jiashu cao Xin Jiang Qinghua Zhang Fusen Yuan Jihao Yu Fan Yang Mingxing Li Chao Wang Ying Lu Ming Li Weihua Wang Yanhui Liu Institute of Physics Chinese Academy of SciencesBeijing 100190People’s Republic of China School of Physical Science University of Chinese Academy of SciencesBeijing 100049People’s Republic of China Songshan Lake Materials Laboratory Guangdong 523808People’s Republic of China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049People’s Republic of China Beijing Advanced Innovation Center for Materials Genome Engineering Beijing 100083People’s Republic of China

Antibacterial activity and mechanical properties of FeCoCr-Ag medium entropy alloys were studied via combinatorial fabrication paired with high-throughput *** was found that the antibacterial activity and mechanical properties exhibit non-linear dependence on the content of Ag *** the studied alloys,(FeCoCr)_(80)Ag_(20) possesses an optimized combination of different properties for potential applications as antibacterial coating *** underlying mechanism is ascribed to the formation of a dual-phase structure that leads to competition between the role of Ag phase and FeCoCr phase at different Ag *** results not only demonstrate the power and effectiveness of combinatorial methods in multi-parameter optimization but also indicate the potential of high entropy alloys as antibacterial materials.

关键词： antibacterial metals and alloys medium entropy alloy combinatorial method

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白菜花茎发育相关基因BcPG17的表达特征分析

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园艺学报 2021年第3期48卷 505-517页

作者：吕美玲曹家树福建农林大学园艺学院福州350002 浙江大学蔬菜研究所细胞与分子生物学实验室杭州310058

在从白菜‘矮脚黄’自交系Bcajh97-01中克隆获得多聚半乳糖醛酸酶基因BcPG17及其启动子序列的基础上,采用qRT-PCR、原位杂交和启动子融合表达载体瞬时转化技术,分析了该基因的表达特征。BcPG17的DNA全长为2105 bp,包含9个外显子和8个内... 详细信息

在从白菜‘矮脚黄’自交系Bcajh97-01中克隆获得多聚半乳糖醛酸酶基因BcPG17及其启动子序列的基础上,采用qRT-PCR、原位杂交和启动子融合表达载体瞬时转化技术,分析了该基因的表达特征。BcPG17的DNA全长为2105 bp,包含9个外显子和8个内含子。ORF序列长度为1344 bp,编码447个氨基酸残基。预测的编码蛋白的相对分子量为48.61kD,理论等电点为8.39,含有1个跨膜结构域,N末端具有信号肽序列,具有被分泌到细胞膜外起作用的特征;其氨基酸序列具有PG蛋白的4个典型结构域,与十字花科芸薹属的其他物种亲缘关系较近。通过qRT-PCR分析发现,BcPG17在开花期花茎中的表达水平最高,且在花粉发育的四分体时期和单核小孢子时期有表达。原位杂交试验结果也显示,BcPG17在开花期花茎的所有组织中均存在强烈的表达信号。通过对克隆获得的1442 bp的BcPG17启动子序列分析,发现该序列含有与分生组织表达有关的顺式作用元件1个、与植物激素响应相关的作用基序和元件多个,以及花药特异基序4个和绒毡层降解延迟蛋白(TDR)结合位点2个;能够启动GUS信号在开花期花茎的第2节间和节处强烈表达,在花发育中期的花药中也存在明显的GUS信号。上述结果表明,BcPG17可能通过与植物激素代谢相关蛋白相互作用调控花茎的伸长,受到绒毡层降解相关蛋白的调控参与花粉的发育。

关键词：白菜 BcPG17 多聚半乳糖醛酸酶花茎表达分析

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拟南芥霜霉病抗性分子机制研究进展

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植物保护 2006年第5期32卷 16-20页

作者：王神云曹家树浙江大学蔬菜研究所杭州310029

随着近年分子生物学技术的发展与应用,植物霜霉病抗性的研究有了长足的进展。本文就拟南芥抗霜霉病基因的克隆与结构分析,抗病信号传导,防卫反应和系统获得抗性,以及寄主-寄生菌共进化冲突方面进行了综述,并就今后的研究进行了展望。

关键词：拟南芥霜霉病抗病性分子生物学

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Oxidation of a Zr_(46)Cu_(46)Al_(7)Gd_(1)bulk metallic glass detected via optical characterizations

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Science China Materials 2021年第4期64卷 973-978页

作者： Weijie Xie Mingxing Li jiashu cao Liwei Hu Chao Wang Wei-Hua Wang Yanhui Liu Institute of Physics Chinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing 100083China

Oxidation of metallic glasses has been shown to be different from crystalline alloys due to the disordered atomic structures and far-from-equilibrium state,and quick characterization is required to reveal their early stage *** this study,we investigated the oxidation behavior of a Zr_(46)Cu_(46)Al_(7)Gd_(1)bulk metallic glass by measuring the changes of optical constants via spectroscopic *** with X-ray diffraction,scanning electron microscopy,and thermogravimetric analyzer confirmed that the changes of pseudo-optical constants reflected not only the onset temperature at which the alloys underwent obvious oxidation,but also the subtle oxidation which was tedious to *** study suggests a more efficient and accurate approach to understand the oxidation of metallic glasses with temperature,time and compositions.

关键词： metallic glasses spectroscopic ellipsometry oxidation

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