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香榧花粉TgPUX10基因克隆与表达

东北林业大学学报 2025年第2期53卷 58-65页

作者：汤天羽汪承巧刘佳乐曹岳英伦张瑞吴家胜省部共建亚热带森林培育国家重点实验室(浙江农林大学) 杭州311300

以香榧(Torreya grandis‘Merrillii’)花粉RNA反转录的cDNA为模板,利用RT-PCR技术分离出一条类似拟南芥PUX10-like cDNA序列,命名为TgPUX10,并对该基因进行鉴定、克隆、表达分析。结果表明:TgPUX10基因的完整开放阅读框(ORF)为1437 bp... 详细信息

以香榧(Torreya grandis‘Merrillii’)花粉RNA反转录的cDNA为模板,利用RT-PCR技术分离出一条类似拟南芥PUX10-like cDNA序列,命名为TgPUX10,并对该基因进行鉴定、克隆、表达分析。结果表明:TgPUX10基因的完整开放阅读框(ORF)为1437 bp,编码477个氨基酸残基。TgPUX10蛋白的氨基酸序列包含UBA、HP、UAS、UBX功能结构域。通过构建瞬时表达载体,并利用拟南芥悬浮细胞转化体系,发现TgPUX10蛋白定位于油体细胞器。在花粉萌发的0、2、4 d时,油体相关基因TgOLE及TgCLO的表达量呈下降趋势,TgPUX10的表达量显著上升,证明TgPUX10影响香榧花粉萌发,且参与油体的降解过程。TgPUX10在各品种香榧中表达较为保守。与其他品种相比,细榧(Torreya grandis‘xifei’)的花粉活性较高,萌发率达到35.5%。

关键词：香榧 TgPUX10基因生物信息学分析亚细胞定位

New insights into the carotenoid biosynthesis in Torreya grandis kernels

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Horticultural Plant Journal 2023年第6期9卷 1108-1118页

作者： Jingwei Yan Hao Zeng Weijie Chen Jiali Luo Congcong Kong Heqiang Lou jiasheng wu State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhouZhejiang 311300China

Torreya grandis is a characteristic rare economic tree species in subtropical mountainous areas. The nut has a variety of bioactive compounds, such as polyphenols and vitamins. However, the carotenoids compositions and its regulatory mechanisms underlying carotenoid biosynthesis in kernels remain unknown. Here, the major carotenoids in the kernel were analyzed. The result showed that lutein and β-carotene were the predominant carotenoids in the kernel, while low levels of a-carotenoid and zeaxanthin were detected. Lutein and β-carotene were decreased during the maturation of kernel. Expression analysis by RNA-seq and q RT-PCR indicated that the expressions of TgCYP97A3 and TgLCYB were also reduced during the maturation of kernel. The contents of lutein or β-carotene were obviously increased in tobacco transiently overexpressing TgCYP97A3 or TgLCYB. Moreover, DPPH radical scavenging activity and FRAP were also significantly enhanced. In addition, several MYB and WRKY transcription factors TgMYBS3, TgMYB48 and TgWRKY11 were identified to positively regulate the TgCYP97A3 expression, while TgMYB48, TgWRKY2 and TgWRKY11 could upregulate the TgLCYB expression. The illustration of carotenoids biosynthesis and its molecular mechanism in kernels not only provides a basis for understanding carotenoids biosynthesis in kernels, but also enables the use of molecular biotechnology to develop new health products rich in carotenoids based on T. grandis nuts.

关键词： Torreya grandis Kernel Lutein b-carotene TgCYP97A3 TgLCYB

Metabolite profiling and transcriptome analyses reveal novel regulatory mechanisms of melatonin biosynthesis in hickory

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Horticulture Research 2021年第1期8卷 2637-2649页

作者： Wenchao Chen Jiaqi Zhang Shan Zheng Zhanqi Wang Chuanmei Xu Qixiang Zhang jiasheng wu Heqiang Lou State Key Laboratory of Subtropical Silviculture Zhejiang A&F University311300 HangzhouZhejiangChina Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province College of Life SciencesHuzhou University313000 HuzhouChina

Studies have shown that melatonin regulates the expression of various elements in the biosynthesis and catabolism of plant *** contrast,the effects of these different plant hormones on the biosynthesis and metabolism of melatonin and their underlying molecular mechanisms are still *** this study,the melatonin biosynthesis pathway was proposed from constructed metabolomic and transcriptomic libraries from hickory(Carya cathayensis Sarg.)*** candidate pathway genes were further identified by phylogenetic analysis,amino-acid sequence alignment,and subcellular ***,most of the transcription factor-related genes coexpressed with melatonin pathway genes were hormone-responsive ***,dual-luciferase and yeast one‐hybrid assays revealed that CcEIN3(response to ethylene)and CcAZF2(response to abscisic acid)could activate melatonin biosynthesis pathway genes,a tryptophan decarboxylase coding gene(CcTDC1)and an N-acetylserotonin methyltransferase coding gene(CcASMT1),by directly binding to their promoters,*** results provide a molecular basis for the characterization of novel melatonin biosynthesis regulatory mechanisms and demonstrate for the first time that abscisic acid and ethylene can regulate melatonin biosynthesis.

关键词： metabolism mechanisms melatonin

Ultrasound activatable antiangiogenic sonosensitizer for VEGFR associated glioblastoma tumor models

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Aggregate 2021年第4期2卷 137-143页

作者： Subin Son Chuangli Zhang Miae Won Paramesh Jangili Minhyeok Choi jiasheng wu Jong Seung Kim Department of Chemistry Korea UniversitySeoulKorea Key Laboratory of Photochemical Conversion and Optoelectronic Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijingChina

Angiogenic signaling pathway is a major contributing factor in cancer recurrence and progression,which can cause significantly reduced treatment outcomes,especially in the oxygen-dependent photo-and sonodynamic *** and its receptor(VEGFR)play a crucial role in angiogenesis progression;precisely,upregulated VEGF signaling ismainly associated with angiogenesis progression in many types of ***,we report a sunitinib-conjugated sonosensitizer(TK-RB:tyrosine kinase-rose bengal)to enhance the anticancer efficacy through VEGF inhibitionmediated antiangiogenesis in conjunction with cellular/tumor damage by ROS generated under ultrasound ***-RB reveals good selectivity and cytotoxicity toward VEGFR-positive cells(U87MG)over VEGFR-negative cells(MCF-7).The fluorescent imaging analysis in vivo/ex vivo and the tumor growth investigation in nude mice with U87MG glioblastoma tumor xenografts demonstrate that rose bengal having tyrosine kinase inhibitor(TK-RB)provides an enhanced antitumor *** current strategy will make a great contribution to optimizing anticancer performance by utilizing sonodynamic therapy together with antiangiogenics in several different malignancies.

关键词： antiangiogenesis glioblastoma sonodynamic therapy VEGFR blockage

Acceptor-planarized typeⅠphotosensitizer for lipid droplet-targeted two-photon photodynamic therapy by ferroptosis

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Science China Chemistry 2024年第7期67卷 2392-2402页

作者： Jie Sha Weimin Liu jiasheng wu Yanping Wang Xuewei Li Haohui Ren Zhi Pang Wenjun Zhang Chun-Sing Lee Pengfei Wang Key Laboratory of Photochemical Conversion and Optoelectronic Materials CityU-CAS Joint Laboratory of Functional Materials and DevicesTechnical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China Center of Super-Diamond and Advanced Films&Department of Materials Science and Engineering City University of Hong KongHong KongChina

Two-photon photodynamic therapy(TP-PDT)has garnered significant attention because of its excellent depth of tissue penetration and high spatiotemporal ***,the limited targeting ability and oxygen dependency of photosensitizers(PSs)significantly hinder the effectiveness of photodynamic therapy in hypoxic tumor ***,we designed and synthesized two lipid droplet(LD)-targeted two-photon PSs(TBPCP and TBCP)by reducing benzene rings to achieve“acceptor planarization”.Notably,acceptor planarization not only enhanced the intramolecular charge transfer but also transferred the photochemical reaction from typeⅡ(TBPCP)to typeⅠ(TBCP).Under the irradiation of 940 nm femtosecond pulsed laser,TBPCP and TBCP showed bright two-photon-excited fluorescence and excellent LD targeting in living *** TBPCP(typeⅡPS),the outstanding TP-PDT efficacy of TBCP(typeⅠPS)under hypoxic conditions could be obtained in both cellular experiments and multicellular tumor spheroids(MCTS)***,both TBPCP and TBCP could induce the lipid peroxidation in the typeⅠor typeⅡPDT due to the location of LD,depleting GSH and inactivating GPX4 to induce nonprogrammed ferroptosis in cells.

关键词： two-photon photodynamic therapy lipid droplet targeting acceptor planarization hypoxia tumor ferroptosis

The effects of different nitrogen sources on camptothecin content and related gene expression in Camptotheca acuminata seedlings

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Journal of Forestry Research 2020年第4期31卷 1347-1357页

作者： Xiaode Wang Sainan Bian Pengjie Chang Ninghang Wang Lingjuan Xuan Mingru Zhang Bin Dong Chao Zhang jiasheng wu Yeqing Ying Xiazhen Lin Yamei Shen School of Landscape Architecture Zhejiang Agriculture and Forestry UniversityHangzhou 310000People’s Republic of China Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang Agriculture and Forestry UniversityHangzhou 310000People’s Republic of China Zhejiang Radio and Television University Hangzhou 310000People’s Republic of China

Camptotheac acuminata Decne is a unique tree species in China with an important secondary metabolite,camptothecin(CPT),used in the treatment of ***(N)is an important element that affects plant growth and the accumulation of *** on the effect of N on CPT synthesis from a genetic perspective are *** explore the effects of different N sources and levels on CPT synthesis in ***,two-year-old seedlings were fertilized with different concentrations of pure ammonium sulphate,source of ammonium N(NH4+-N),and potassium nitrate for nitrate N(NO3--N).Concentrations of 2.5,5,7.5,and10 g pot-1 NH4+-N and NO3--N were *** results showed that 7.5 g NH4+-N and NO3--N treatments were best for growth and fresh weight of *** with the other treatments,the CPT content,tryptophan synthase and tryptophan decarboxylase activities,and expression of the CaTSB and CaTDCl genes under the 2.5 g NH4+-N and NO3--N treatments peaked significantly at 30 ***,the expression of CaTDC2 surpassed that of the other two genes at 60 ***,compared with NH4+-N source,the NO3--N source was more beneficial for growth,and NO3--N was better for CPT ***,leaves of *** treated with 2.5 g NO3--N could be harvested after 30 days to obtain maximum CPT ***1 is more closely linked to CPT *** results of this study improved the production of CPT in *** via fertilization.

关键词： Ammonium sulfate Camptotheca acuminata Camptothecin Gene expression Potassium nitrate

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淹水胁迫对杨桐幼苗生理生化性质的影响

东北林业大学学报 2015年第4期43卷 34-36页

作者：吴江吴家胜黄岩区林业特产局黄岩318020 亚热带森林培育国家重点实验室培育基地(浙江农林大学)

以1年生杨桐实生苗为试验材料,设置4个梯度的淹水胁迫,分析其生理生化指标对淹水胁迫的响应。结果表明:淹水条件下杨桐超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、质膜透性、可溶性蛋白质量分数、可溶性糖质量分数显著上升,分别比... 详细信息

以1年生杨桐实生苗为试验材料,设置4个梯度的淹水胁迫,分析其生理生化指标对淹水胁迫的响应。结果表明:淹水条件下杨桐超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、质膜透性、可溶性蛋白质量分数、可溶性糖质量分数显著上升,分别比对照上升了75.0%、30.0%、25.1%、140.3%和101.6%,叶绿素a、叶绿素b质量分数显著下降,分别比对照下降39.8%和29.3%。w(叶绿素a)∶w(叶绿素b)和相对含水量有不同程度的降低,但不显著。对9个抗涝指标进行主成分分析,发现3个主成分贡献率为88.58%。

关键词：杨桐幼苗淹水胁迫渗透调节保护酶

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知识型制造企业的组织架构模型探讨

科技管理研究 2005年第12期25卷 194-196页

作者：吴加胜董雨中国科学技术大学人文与社会科学学院中国科学技术大学管理学院安徽合肥230026

本文从分析知识经济的特征入手,引入知识型企业两种形态:知识型服务企业和知识型制造企业,讨论了知识型制造企业组织架构的原则,分别从知识团队组织架构设计、高层团队组织架构设计和功能性专家团队架构设计三方面对知识型制造企业组织... 详细信息

本文从分析知识经济的特征入手,引入知识型企业两种形态:知识型服务企业和知识型制造企业,讨论了知识型制造企业组织架构的原则,分别从知识团队组织架构设计、高层团队组织架构设计和功能性专家团队架构设计三方面对知识型制造企业组织架构进行了详细的探讨,提出了一个知识型制造企业的组织架构模型。

关键词：知识管理知识型企业制造业组织架构

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能源塔波纹填料间气液两相热质交换模拟

南京理工大学学报 2018年第1期42卷 26-32页

作者：吴慧华曹琳吴加胜南京理工大学能源与动力工程学院江苏南京210094

为了提高能源塔吸热过程塔内波纹填料间液相降膜与逆流气相的热质交换性能,该文分析了波纹填料间气液两相热质交换过程,建立了波纹填料间气液两相逆流接触热质交换模型,模拟了不同气液相入口参数时填料间气液两相热质交换过程,发现相较... 详细信息

为了提高能源塔吸热过程塔内波纹填料间液相降膜与逆流气相的热质交换性能,该文分析了波纹填料间气液两相热质交换过程,建立了波纹填料间气液两相逆流接触热质交换模型,模拟了不同气液相入口参数时填料间气液两相热质交换过程,发现相较于改变其它气液相入口参数,增大气相入口流量能在显著提高气液两相间传热传质量的同时减小潜热比,从提高换热量并减缓溶液稀释的角度出发,是改善能源塔换热性能的好方法。

关键词：能源塔逆流波纹填料气液两相热质交换数值模拟