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Comparative genomic analyses reveal cis-regulatory divergence after polyploidization in cotton

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The Crop Journal 2022年第6期10卷 1545-1556页

作者： jiaqi you Min Lin Zhenping Liu Liuling Pei Yuexuan Long Lili Tu Xianlong Zhang Maojun Wang National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070HubeiChina School of Pharmaceutical Sciences South-Central University for NationalitiesWuhan 430074HubeiChina

Polyploidization has long been recognized as a driver for the evolutionary formation of superior plant traits coupled with gene expression ***,knowledge of the effect of regulatory variation on expression changes following polyploidization remains *** this study,we characterized transcriptional regulatory divergence by comparing tetraploid cotton with its putative diploid *** identified 144,827,99,609,and 219,379 Tn5 transposase-hypersensitive sites(THSs)in Gossypium arboreum,***,and ***,respectively,and found that the conservation of promoter THSs was associated with coordination of orthologous genes *** observation was consistent with analysis of transcription-factor binding sites(TFBS)for 262 known motifs:genes with higher TFBS conservation scores(CS)showed less change than those genes with lower TFBS CS in expression *** influenced by genomic variation were involved in the novel regulation networks between transcriptional factors and target genes in tetraploid *** describe an example showing that the turnover of TFBS was linked to expression pattern divergence of genes involved in fiber development(fiber-related genes).Our findings reveal the regulatory divergence of the transcriptional network in cotton after polyploidization and characterizes the regulatory relationships of genes contributing to desirable traits.

关键词： Cotton Polyploidization Transcriptional regulation Fiber development

Phenylpropanoid metabolism and pigmentation show divergent patterns between brown color and green color cottons as revealed by metabolic and gene expression analyses

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Journal of Cotton Research 2020年第4期3卷 239-249页

作者： LI Zhonghua SU Qian XU Mingqi you jiaqi KHAN Anam Qadir LI Junyi ZHANG Xianlong TU Lili you Chunyuan National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Cotton Research Institute Shihezi Academy of Agriculture ScienceShihezi 832000XinjiangChina

Background:Naturally-colored cotton has become increasingly popular because of their natural properties of coloration,UV protection,flame retardant,antibacterial activity and mildew *** poor fiber quality and limited color choices are two key issues that have restricted the cultivation of naturally-colored *** identify the possible pathways participating in fiber pigmentation in naturally-colored cottons,five colored cotton accessions in three different color types(with green,brown and white fiber)were chosen for a comprehensive analysis of phenylpropanoid metabolism during fiber ***:The expression levels of flavonoid biosynthesis pathway genes in brown cotton fibers were significantly higher than those in white and green cotton *** flavonoids and proanthocyanidin were higher in brown cotton fibers relative to those in white and green cotton fibers,which suggested that the flavonoid biosynthesis pathway might not participate in the pigmentation of green cotton *** expression analysis indicated that the genes encoding enzymes for the synthesis of caffeic acid derivatives,lignin and lignan were activated in the developing fibers of the green cotton at 10 and 15 days ***:Our results strengthen the understanding of phenylpropanoid metabolism and pigmentation in green and brown cotton fibers,and may improve the breeding of naturally-colored cottons.

关键词： Naturally-colored cotton Flavonoids Lignin Lignan Suberin

Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2025年第01期 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao jiaqi Wang Weilan Wu Linlin you Yonggui Yuan Qian Xu Rong Gao Key Laboratory of Environmental Medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University Department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

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拟康宁木霉T-51几丁质酶活性及内切几丁质酶基因克隆与分析

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植物保护 2017年第4期43卷 21-27页

作者：尤佳琪李国庆华中农业大学作物病害监测和安全控制湖北省重点实验室武汉430070

木霉是一类重要的生防真菌,木霉产生的几丁质酶在其生物防治的重寄生过程中起着重要作用。拟康宁木霉Trichoderma koningiopsis T-51是一株对番茄灰霉病有生防潜力的木霉菌株,本文测定了T-51菌株产生几丁质酶的活性,结果表明T-51在PDB... 详细信息

木霉是一类重要的生防真菌,木霉产生的几丁质酶在其生物防治的重寄生过程中起着重要作用。拟康宁木霉Trichoderma koningiopsis T-51是一株对番茄灰霉病有生防潜力的木霉菌株,本文测定了T-51菌株产生几丁质酶的活性,结果表明T-51在PDB中液体培养以及与灰霉病菌在PDA上对峙培养时产生的几丁质酶活性显著受到灰霉病菌的诱导。采用RACE技术首次克隆了拟康宁木霉T-51中一个几丁质酶基因Tkchit42,全长为1 817bp,包含4个外显子和3个内含子。预测该基因有一个1 275bp的开放阅读框,编码424个氨基酸,预测的蛋白总分子量为46.378kDa,预测的等电点(pI)为5.16,与***中42kDa内切几丁质酶的氨基酸序列相似性达到99%。

关键词：几丁质酶拟康宁木霉基因克隆序列分析

拟康宁木霉T-51菌株发酵液对灰葡萄孢的抑菌活性

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植物保护 2021年第1期47卷 74-78,83页

作者：尤佳琪李国庆上海市农业科学院园艺研究所上海201106 华中农业大学农业微生物学国家重点实验室武汉430070

拟康宁木霉菌株T-51是从土壤中分离得到的一株优秀生防菌株,在前期的研究与应用中表现出对番茄灰霉病的良好防效。本文研究了T-51菌株的PDB发酵液对灰葡萄孢的抑菌活性,结果表明T-51发酵液在0.5%的低浓度下的抑菌率达到52.1%,与高浓度(1... 详细信息

拟康宁木霉菌株T-51是从土壤中分离得到的一株优秀生防菌株,在前期的研究与应用中表现出对番茄灰霉病的良好防效。本文研究了T-51菌株的PDB发酵液对灰葡萄孢的抑菌活性,结果表明T-51发酵液在0.5%的低浓度下的抑菌率达到52.1%,与高浓度(10%)发酵液的抑菌率(59.7%)没有显著差异。当发酵液浓度下降为0.05%时失去抑菌活性。T-51摇培至第5天时的发酵液的抑菌活性稳定。T-51发酵液中的抑菌物质对高温不敏感,沸水浴处理10 min后抑菌活性没有明显降低。T-51发酵液pH为3.3,呈酸性,当调节pH至7时,发酵液抑菌活性从57.89%下降到46.61%。T-51发酵液能够显著抑制灰葡萄孢侵染番茄叶片后产生的病斑大小,接种后叶片病斑直径比对照减小53.9%。

关键词：拟康宁木霉T-51 灰葡萄孢生物防治抗真菌活性

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论预防金融职务犯罪的意义和途径

中国农业银行武汉培训学院学报 2003年第2期 58-59页

作者：游家齐湖北省宜昌市农村信用合作联社湖北宜昌430300

本文研究探讨金融职务犯罪及其预防问题

关键词：预防金融职业犯罪意义与途径

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基于模糊核优化的图像盲去模糊算法

电子科技 2018年第1期31卷 54-57,74页

作者：尤佳旗上海理工大学机械工程学院上海200093

针对图像的模糊算法优化问题,首先选取高斯分布拟合自然图像的分布特性,利用双边滤波器从模糊图像中提取出清晰的图像边缘。针对降噪进行参数设置,在初步估计出模糊核之后,对模糊核进行正规化修正工作。最后在图像复原阶段,利用优化的... 详细信息

针对图像的模糊算法优化问题,首先选取高斯分布拟合自然图像的分布特性,利用双边滤波器从模糊图像中提取出清晰的图像边缘。针对降噪进行参数设置,在初步估计出模糊核之后,对模糊核进行正规化修正工作。最后在图像复原阶段,利用优化的凸函数拟合自然图像分布,并利用快速傅里叶变换提高算法计算速度。实验结果,表明该模糊核优化算法与现有的其他算法相比,复原后的图像具有更好的视觉效果,且计算时间减少约20%。

关键词：高斯分布滤波器模糊核傅里叶变换

Tubastatin A通过降低氧化应激减轻脓毒症所致心肌损伤的体外研究

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中国医科大学学报 2023年第7期52卷 633-637页

作者：尤佳琪刘畅崇巍中国医科大学附属第一医院急诊科沈阳110001

目的探讨Tubastatin A(Tub A)对脂多糖(LPS)刺激RAW264.7巨噬细胞导致心肌细胞氧化应激损伤的作用。方法将H9C2心肌细胞分为3组:Sham组(用无LPS刺激的巨噬细胞所获上清液培养心肌细胞)、MCM组(用LPS刺激的巨噬细胞所获上清液培养心肌细... 详细信息

目的探讨Tubastatin A(Tub A)对脂多糖(LPS)刺激RAW264.7巨噬细胞导致心肌细胞氧化应激损伤的作用。方法将H9C2心肌细胞分为3组:Sham组(用无LPS刺激的巨噬细胞所获上清液培养心肌细胞)、MCM组(用LPS刺激的巨噬细胞所获上清液培养心肌细胞,建立体外脓毒症心肌损伤模型)、Tub A组(Tub A预处理后,再用含Tub A经LPS刺激的巨噬细胞所获上清液培养心肌细胞)。3组心肌细胞培养24 h后检测细胞活性、细胞氧化应激水平及心肌损伤标志物。结果与Sham组比较,MCM组巨噬细胞上清液中一氧化氮水平显著增高(P=0.0001);MCM组心肌细胞活性氧(ROS)、脂质过氧化产物丙二醛(MDA)含量显著升高,而心肌细胞活性明显下降,心肌细胞释放的乳酸脱氢酶(LDH)及心肌型肌酸激酶同工酶明显升高(均P<0.05)。与MCM组比较,Tub A组ROS、MDA明显下降,细胞活性明显升高,LDH明显下降(均P<0.05)。结论Tub A可以减轻LPS刺激巨噬细胞导致的心肌细胞损伤,其作用机制可能是通过降低氧化应激来完成的。

关键词： Tubastatin A 脓毒症心肌细胞氧化应激体外

超声振动磨削氧化锆陶瓷表面损伤模型的实验研究

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工具技术 2022年第6期56卷 52-56页

作者：尤佳旗黄久超丁霖上海航天精密机械研究所上海市201600

以压痕断裂力学模型和硬脆材料去除机理为理论基础,对氧化锆陶瓷的表面损伤进行研究,结合砂轮特征及磨削力公式建立了普通磨削和超声振动磨削的横向裂纹和表面破碎率模型,并对实验所得材料表面进行图像处理。结果表明:横向裂纹和表面破... 详细信息

以压痕断裂力学模型和硬脆材料去除机理为理论基础,对氧化锆陶瓷的表面损伤进行研究,结合砂轮特征及磨削力公式建立了普通磨削和超声振动磨削的横向裂纹和表面破碎率模型,并对实验所得材料表面进行图像处理。结果表明:横向裂纹和表面破碎率随着磨削深度增大而增大,与普通磨削相比,超声振动磨削降低了45%裂纹长度,降低了23%表面破碎率,具有更完整的表面形貌特征。

关键词：超声振动磨削横向裂纹表面破碎率