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bZIP71 delays flowering by suppressing Ehd1 expression in rice

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Journal of Integrative Plant Biology 2022年第7期64卷 1352-1363页

作者： Xiufeng Li Xiaojie Tian Mingliang He Xinxin Liu Zhiyong Li jiaqi tang Enyang Mei Min Xu Yingxiang Liu Zhenyu Wang Qingjie Guan Wei Meng Jun Fang Jian Zhang Qingyun Bu Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design Breedingthe Chinese Academy ofSciencesHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China College of Life Science Northeast Forestry UniversityHarbin 150040China Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China State Key Lab of Rice Biology China National Rice Research InstituteHangzhou 311400China The Innovative Academy of Seed Design the Chinese Academy of SciencesBeijing 100101China

Flowering time is a fundamental factor determining the global distribution and final yield of rice(Oryza sativa).Although diverse flowering time genes have been reported in this crop,the transcriptional regulation of its key flowering genes are poorly understood.Here,we report that a basic leucine zipper transcription factor,bZIP71,functions as a flowering repressor.The overexpression of bZIP71 delays flowering,while the bzip71 mutant flowers early in both long-day and short-day conditions.A genetic analysis showed that the regulation of flowering by bZIP71 might be independent of Heading date 2(Hd2),Hd4,and Hd5.Importantly,bZIP71 directly associates with the Early heading date 1(Ehd1)promoter and represses its transcription,and genetically the function of bZIP71 is impaired in the ehd1 mutant.Moreover,bZIP71 interacts with major components of polycomb repressive complex 2(PRC2),SET domain group protein 711(SDG711),and Fertilization independent endosperm 2(FIE2),through which bZIP71 regulates the H3K27me3 level of Ehd1.Taken together,we present a transcriptional regulatory mechanism in which bZIP71 enhances the H3K27me3 level of Ehd1 and transcriptionally represses its expression,which not only offers a novel insight into a flowering pathway,but also provides a valuable putative target for the genetic engineering and breeding of elite rice cultivars.

关键词： flowering time H3K27me3 rice transcriptional regulation

Oryza sativa mediator subunit OsMED25 interacts with OsBZR1 to regulate brassinosteroid signaling and plant architecture in rice

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Journal of Integrative Plant Biology 2020年第6期62卷 793-811页

作者： Yuekun Ren Xiaojie Tian Shuyu Li Enyang Mei Mingliang He jiaqi tang Min Xu Xiufeng Li Zhenyu Wang Chuanyou Li Qingyun Bu Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design BreedingChinese Academy of SciencesHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Brassinosteroids(BRs)are plant-specific steroid hormones which regulate plant growth,development,and adaptation.Transcriptional regulation plays key roles in plant hormone signaling.A mediator can serve as a bridge between gene-specific transcription factors and the RNA polymerase machinery,functioning as an essential component in regulating the transcriptional process.However,whether a mediator is involved in BR signaling is unknown.Here,we discovered that Oryza sativa mediator subunit 25(Os MED25)is an important regulator of rice BR signaling.Phenotypic analyses showed that the Os MED25-RNAi and osmed25 mutant presented erect leaves,as observed in BR-deficient mutants.In addition,the Os MED25-RNAi and osmed25 mutant exhibited decreased BR sensitivity.Genetic analysis indicated that Os MED25-RNAi could suppress the enhanced BR signaling phenotype of Osbzr1-D.Further biochemical analysis showed that Os MED25 interacts with Os BZR1 in vivo,and Os MED25 is enriched on the promoter of Os BZR1 target genes.RNA sequencing analysis indicated that Os MED25 affects the expression of approximately 45%of Os BZR1-regulated genes and mainly functions as a corepressor of Os BZR1.Together,these findings revealed that Os MED25 regulates rice BR signaling by interacting with Os BZR1 and modulating the expression of Os BZR1 target genes,thus expanding our understanding of the roles of mediators in plant hormone signaling.

关键词： plant Together analysis

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调查研究在统战工作中的运用和创新研究

湖南省社会主义学院学报 2024年第2期25卷 65-70页

作者：周批改唐佳琪湘潭大学公共管理学院、南亚研究中心湖南湘潭411105

中国共产党在统战工作中始终高度重视调查研究,淬炼出了统战调研的系列宝贵经验。中国特色社会主义进入新时代,统一战线处于新的历史方位,肩负新的时代使命,迫切需要通过调查研究来把握统战发展方向、完善统战理论政策、开创统战工作新... 详细信息

中国共产党在统战工作中始终高度重视调查研究,淬炼出了统战调研的系列宝贵经验。中国特色社会主义进入新时代,统一战线处于新的历史方位,肩负新的时代使命,迫切需要通过调查研究来把握统战发展方向、完善统战理论政策、开创统战工作新局面、纠正统战调研实践偏差。做好新时代统战调研工作,必须校准靶向,选好统战调研焦点课题;求真务实,推动统战调研走深走实;创新拓展,促进统战调研取得实效;内外联动,保障统战调研常态开展。

关键词：统一战线调查研究守正创新

OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4-OsMAPK6-OsWRKY53 signaling pathway

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Journal of Integrative Plant Biology 2021年第12期63卷 2043-2057页

作者： Zhiqi Liu Enyang Mei Xiaojie Tian Mingliang He jiaqi tang Min Xu Jiali Liu Lu Song Xiufeng Li Zhenyu Wang Qingjie Guan Qijiang Xu Qingyun Bu Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China College of Life Science Northeast Forestry UniversityHarbin 150040China Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design BreedingCASHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China School of Laboratory Medicine Youjiang Medical University for NationalitiesBaise 533000China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Grain size and leaf angle are key agronomic traits that determine final yields in rice.However,the underlying molecular mechanisms are not well understood.Here we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK70 regulates grain size and leaf angle in rice.Overexpressing OsMKKK70 caused plants to produce longer seeds.The osmkkk62/70 double mutant and the osmkkk55/62/70 triple mutant displayed significantly smaller seeds and a more erect leaf angle compared to the wild type,indicating that OsMKKK70 functions redundantly with its homologs Os MKKK62 and Os MKKK55.Biochemical analysis demonstrated that OsMKKK70 is an active kinase and that OsMKKK70 interacts with Os MKK4 and promotes Os MAPK6 phosphorylation.In addition,the osmkkk62/70 double mutant showed reduced sensitivity to Brassinosteroids(BRs).Finally,overexpressing constitutively active Os MKK4,Os MAPK6,and Os WRKY53 can partially complement the smaller seed size,erect leaf,and BR hyposensitivity of the osmkkk62/70 double mutant.Taken together,these findings suggest that OsMKKK70 might regulate grain size and leaf angle in rice by activating Os MAPK6 and that OsMKKK70,Os MKK4,Os MAPK6,and Os WRKY53 function in a common signaling pathway that controls grain shape and leaf angle.

关键词： Brassinosteroids MAPK signaling OsMKKK70 rice seed size

鱼骨型分支井SAGD分支形态优化及采出程度变化规律

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特种油气藏 2024年第1期31卷 57-65页

作者：周志军张祺衣犀唐佳琦张国青东北石油大学提高油气采收率教育部重点实验室黑龙江大庆163318

风城油田低物性段普遍发育,储层非均质性严重。鱼骨型分支井SAGD通过分支深入储集层可改善常规SAGD蒸汽腔扩展受限的问题。为了达到最佳开采效果,有必要对鱼骨型分支井SAGD的分支形态进行优化,并掌握影响采出程度变化的规律。为此,利用... 详细信息

风城油田低物性段普遍发育,储层非均质性严重。鱼骨型分支井SAGD通过分支深入储集层可改善常规SAGD蒸汽腔扩展受限的问题。为了达到最佳开采效果,有必要对鱼骨型分支井SAGD的分支形态进行优化,并掌握影响采出程度变化的规律。为此,利用油藏数值模拟及正交实验设计的手段,确定分支参数对提高采出程度具有敏感性,并优化分支参数,分析不同储层物性下分支参数对采出程度的影响规律。将研究成果应用于风城油田,该研究区块的最佳井型部署方案为:分支长度为120 m、分支数量为4条、分支角度为75°、分支异侧为非对称分布、分支间距为100 m。通过研究可知,各分支参数的影响权重以储层渗透率2.0 D为明显的分界点:当储层渗透率小于2.0 D时,主要影响因素为分支长度、分支数量;储层渗透率为2.0 D时,分支角度、分支间距的作用开始变强。研究成果对风城油田SAGD驱进一步提高采收率具有重要的指导意义。

关键词：鱼骨型分支井SAGD 分支形态优化数值模拟提高采收率风城油田

Measuring Dependence Risk of Funds with Copula in China

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Applied Mathematics 2014年第13期5卷 1863-1869页

作者： jiaqi tang Guohua Sun College of Science Guilin University of Technology Guilin China School of Management Guilin University of Technology Guilin China

The aim of this paper is to measure dependence risk of fund market with copulas in China. Firstly, we introduce several common copula functions, then estimate parameters of copula function, and discuss how to select the optimal copula function. Finally, according to Shanghai and Shenzhen fund data, empirical analysis was done. Different combinations of risk values were obtained.

关键词： Copula Dependence Risk Monte Carlo Simulation Parameter Estimation

在岸与离岸人民币汇率多尺度溢出效应研究——基于MODWT-DGCt-MSV模型

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金融与经济 2024年第4期 66-76页

作者：汤家奇严志勇江国良戴锐豪王雪茹中国人民银行江西省分行中国人民银行鹰潭市分行中国人民银行上饶市分行中国人民银行萍乡市分行

随着人民币国际化的稳慎扎实推进,在岸和离岸人民币汇率双向波动逐渐成为常态,市场风险传递较以往也愈发迅速。构建MODWT-DGCt-MSV模型,测量在岸与离岸人民币汇率在不同频次交易下形成多个尺度的波动溢出效应。研究表明,离岸人民币汇率... 详细信息

随着人民币国际化的稳慎扎实推进,在岸和离岸人民币汇率双向波动逐渐成为常态,市场风险传递较以往也愈发迅速。构建MODWT-DGCt-MSV模型,测量在岸与离岸人民币汇率在不同频次交易下形成多个尺度的波动溢出效应。研究表明,离岸人民币汇率的波动性比在岸更强,在岸人民币汇率长期尺度与离岸人民币汇率中期、长期的动态相关性较强;在岸和离岸人民币汇率波动存在明显的集聚效应,各尺度之间大多存在波动传递的格兰杰因果关系,离岸人民币市场更多地作为波动溢出的源头;在岸人民币汇率在美联储加息时对离岸的波动溢出效应较非加息周期有所增强。

关键词：小波分析波动溢出资本流动宏观审慎

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AZ80挤压镁合金微弧涂层的耐蚀性能

金属热处理 2024年第1期49卷 256-261页

作者：汤嘉琪薛玉娜王灿坤申毅刘晓龙姬蕴卓方烨王博瑶西安石油大学材料科学与工程学院陕西西安710065 新疆油田公司工程技术处新疆克拉玛依834009 西安福莱特热处理有限公司陕西西安710065

针对镁合金裸露动件的服役环境及表面防护特点,采用微弧氧化(MAO)表面处理技术在AZ80挤压合金表面制备MAO涂层,通过连续盐雾加速腐蚀试验,研究了AZ80合金基体及MAO涂层的静态腐蚀性能,并对AZ80合金基体和MAO涂层试样进行预腐蚀,通过慢... 详细信息

针对镁合金裸露动件的服役环境及表面防护特点,采用微弧氧化(MAO)表面处理技术在AZ80挤压合金表面制备MAO涂层,通过连续盐雾加速腐蚀试验,研究了AZ80合金基体及MAO涂层的静态腐蚀性能,并对AZ80合金基体和MAO涂层试样进行预腐蚀,通过慢应变速率拉伸试验研究了MAO涂层及预腐蚀时间对AZ80镁合金腐蚀行为的影响。结果表明,MAO涂层具有较好的静态防护性能,盐雾腐蚀6天后出现点蚀现象,涂层破裂后的腐蚀行为取决于AZ80合金基体的耐蚀性。在慢应变速率拉伸状态下,随着预腐蚀时间的延长,AZ80基体和MAO涂层的应力腐蚀敏感性增加,MAO涂层的应力腐蚀敏感性指数明显小于AZ80合金基体。MAO涂层试样对AZ80基体有明显的防护作用,可有效提高镁合金的耐蚀性。

关键词： AZ80挤压镁合金微弧氧化盐雾腐蚀应力腐蚀

一种筛选水稻Mutmap+突变位点的简易方法

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扬州大学学报（农业与生命科学版） 2024年第1期45卷 27-32页

作者：王延妍李颖颖王子瑞毛馨晨唐家琪于恒秀张超扬州大学农学院/江苏省作物基因组学和分子育种重点实验室/植物功能基因组学教育部重点实验室江苏扬州225009

利用基因组重测序技术进行基因定位目前已在水稻功能基因组学研究中得到广泛应用。其中,Mutmap+技术因无需进行杂交操作,且无需背景亲本的基因组信息,具有更广阔的应用前景。然而,目前Mutmap+技术筛选候选突变位点的方法依赖于复杂的数... 详细信息

利用基因组重测序技术进行基因定位目前已在水稻功能基因组学研究中得到广泛应用。其中,Mutmap+技术因无需进行杂交操作,且无需背景亲本的基因组信息,具有更广阔的应用前景。然而,目前Mutmap+技术筛选候选突变位点的方法依赖于复杂的数据计算,要求研究者具有较高的生物信息学知识。根据Mutmap+的实验原理,设计一种简单的筛选候选突变位点的方法。该方法对混池测序的表型池与非表型池的突变指数分别加以限定,即表型池突变指数等于1,非表型池突变指数小于0.5。对测序所得突变位点进行简单排序,即可得到候选突变位点。运用该筛选方法,从6个水稻不育突变体成功克隆突变基因,并对其中1个突变体进行了细胞学表型的验证。该方法可简化Mutmap+技术的数据分析流程,便于将Mutmap+技术更好地运用到水稻功能基因组学研究中。

关键词：水稻 Mutmap+ 突变位点筛选基因克隆