检索结果-南通市图书馆

Genome transfer for the prevention of female infertility caused by maternal gene mutation

Journal of Genetics and Genomics 2020年第6期47卷 311-319页

作者： Dandan Bai Jin Sun Yanping Jia Jiqing Yin Yalin Zhang Yanhe Li Rui Gao Xiling Du Kunming Li Jiaming Lin Zhifen Tu Yu Wang jiaping pan shanshan liang Yi Guo Jingling Ruan Xiaochen Kou Yanhong Zhao Hong Wang Cizhong Jiang Fengchao Wang Xiaoming Teng Wenqiang Liu Shaorong Gao Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital.Shanghai Key Laboratory of Signaling and Disease Research Frontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai200092China National Institute of Biological Sciences.NIBS.Beijing 102206China

Poor oocyte quality is associated with early embryo developmental arrest and *** gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female ***,how to improve oocyte quality and develop effective therapy for maternal gene mutation remains ***,we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutationecaused female *** first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation(ZGA)during the maternal-zygotic *** next perform genome transfer at the oocyte(spindle transfer or polar body transfer)and zygote(early pronuclear transfer or late pronuclear transfer)stages to validate the feasibility of preventing Zar1 mutationecaused *** finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live ***,those pups grow to adulthood and show normal ***,our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation.

关键词： Infertility Oocytes Zar1 Maternal-zygotic transition ZGA Spindle transfer Genome transfer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Costunolide covalently targets NACHT domain of NLRP3 to inhibit inflammasome activation and alleviate NLRP3-driven inflammatory diseases

引用

Acta Pharmaceutica Sinica B 2023年第2期13卷 678-693页

作者： Haowen Xu Jiahao Chen pan Chen Weifeng Li Jingjing Shao shanshan Hong Yi Wang Lingfeng Chen Wu Luo Guang liang Chemical Biology Research Center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035China School of Pharmaceutical Sciences Hangzhou Medical CollegeHangzhou 311399China Medical Research Center the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China

The NLRP3 inflammasome’s core and most specific protein,NLRP3,has a variety of functions in inflammation-driven ***(COS)is the major active ingredient of the traditional Chinese medicinal herb Saussurea lappa and has anti-inflammatory activity,but the principal mechanism and molecular target of COS remain ***,we show that COS covalently binds to cysteine 598 in NACHT domain of NLRP3,altering the ATPase activity and assembly of NLRP3 *** declare COS’s great anti-inflammasome efficacy in macrophages and disease models of gouty arthritis and ulcerative colitis via inhibiting NLRP3 inflammasome *** also reveal that theα-methylene-γ-butyrolactone motif in sesquiterpene lactone is the certain active group in inhibiting NLRP3 *** together,NLRP3 is identified as a direct target of COS for its anti-inflammasome ***,especially theα-methylene-γ-butyrolactone motif in COS structure,might be used to design and produce novel NLRP3 inhibitors as a lead compound.

关键词： Costunolide NLRP3 inflammasome Inflammation Molecular target NACHT domain α-Methylene-γ-butyrolactone Gouty arthritis Ulcerative colitis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Cloning and Bioinformatics Analysis of TypeⅢSecretion System Effector Protein HY 322 Gene of Vibrio alginolyticus

引用

Agricultural Biotechnology 2018年第6期7卷 1-5,8页

作者： shanshan liang Mingjie FAN Weijian liang Huanying panG Chuanhao pan Dawei SONG Mingsheng QIU Jichang JIAN Shenzhen Institute of Guangdong Ocean University Shenzhen 510000China Fisheries College of Guangdong Ocean University Zhanjiang524088China Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemilogy for Aquatic Economic Animals/Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524088China

In this study,Hy322 gene was cloned from Vibrio *** total length of its gene was 969 bp,and it could encode 322 amino *** physicochemical properties,protein structure,genetic evolutionary relationship and antigenic characteristics of the effector protein Hy322 of *** HY9901 type Ⅲ secretion system were studied and analyzed by bioinformatics methods and *** results showed that Hy322 is an unstable hydrophilic and acidic protein without a transmembrane region and a signal peptide,and secondary structure to α-*** evolutionary analysis showed that *** HY9901 and *** were clustered together,which indicated that the genetic relationship between the two species was ***322 contains a FliN super family conserved domain associated with Flagellar motor *** analysis showed that the B-cell preponderant epitopes of Hy322 might be localized in the regions of 32-33,100-102,138-140,215-216,235-238 and *** 3D structure model of Hy322 subunit was simulated by SWISS-MODEL software and itwas found that the yscQ of Yersinia were similar and the similarity was 42.25%.In this study,the feasibility of Hy322 as a common antigen of Vibrio was verified from the perspective of bioinformatics,which laid the foundation for the next step in vaccine development.

关键词： Vibrio alginolyticus TypeⅢsecretory system Effector protein Bioinformatics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microbiota-mediated shaping of mouse spleen structure and immune function characterized by scRNA-seq and Stereo-seq

引用

Journal of Genetics and Genomics 2023年第9期50卷 688-701页

作者： Yin Zhang Juan Shen Wei Cheng Bhaskar Roy Ruizhen Zhao Tailiang Chai Yifei Sheng Zhao Zhang Xueting Chen Weiming liang Weining Hu Qijun Liao shanshan pan Wen Zhuang Yangrui Zhang Rouxi Chen Junpu Mei Hong Wei Xiaodong Fang College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China BGI-Shenzhen ShenzhenGuangdong 518083China College of Animal Sciences and Technology Huazhong Agricultural UniversityWuhanHubei 430070China BGI-Qingdao BGI-ShenzhenQingdaoShandong 266555China BGI-Sanya BGI-ShenzhenSanyaHainan 572025China Precision Medicine Institute The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510080China

Gut microbes exhibit complex interactions with their hosts and shape an organism's immune system throughout its *** the largest secondary lymphoid organ,the spleen has a wide range of immunological *** explore the role of microbiota in regulating and shaping the spleen,we employ scRNA-seq and Stereo-seq technologies based on germ-free(GF)mice to detect differences in tissue size,anatomical structure,cell types,functions,and spatial molecular *** identify 18 cell types,9 subtypes of T cells,and 7 subtypes of B *** differential expression analysis reveals that the absence of microorganisms results in alterations in erythropoiesis within the red pulp region and congenital immune deficiency in the white pulp ***-seq results demonstrate a clear hierarchy of immune cells in the spleen,including marginal zone(MZ)macrophages,MZ B cells,follicular B cells and T cells,distributed in a well-defined pattern from outside to ***,this hierarchical structure is disturbed in GF ***7 and Cxcl13 chemokines are specifically expressed in the spatial locations of T cells and B cells,*** speculate that the microbiota may mediate the structural composition or partitioning of spleen immune cells by modulating the expression levels of chemokines.

关键词： ScRNA-seq Spatial transcriptome GF mouse Spleen structure Microbiota Immune function

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于特征再分解的数据稀疏表示

引用

北京工业大学学报 2017年第11期43卷 1666-1672页

作者：高亮于佳平潘积远西安理工大学自动化与信息工程学院西安710048 东华大学理学院上海201620 中国电子科技集团公司第二十研究所西安710068

针对因非负矩阵分解模型目标函数非凸而出现局部次优基特征平滑的现象,提出基于特征再分解的数据稀疏表示方法,在多种先验正则信息约束下初步挖掘原始数据的潜在特征,再秉承非负加性线性表示方式的"局部构成整体"的认知优势,利用非负矩... 详细信息

针对因非负矩阵分解模型目标函数非凸而出现局部次优基特征平滑的现象,提出基于特征再分解的数据稀疏表示方法,在多种先验正则信息约束下初步挖掘原始数据的潜在特征,再秉承非负加性线性表示方式的"局部构成整体"的认知优势,利用非负矩阵分解对特征突显的信息再次凝练,获取数据潜在本征信息,实现非负数据稀疏表示.算法在合成的Swimmer和人脸图像数据的实验结果表明,与传统非负矩阵分解方法相比,该方法的基特征稀疏性得到增强,且判别能力也获得显著提高.

关键词：非负矩阵分解特征再分解稀疏表示人脸识别

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Famitinib in combination with concurrent chemoradiotherapy in patients with locoregionally advanced nasopharyngeal carcinoma: a phase 1, open-label, dose-escalation Study

引用

Cancer Communications 2018年第1期38卷 701-713页

作者： Qiuyan Chen Linquan Tang Na Liu Feng Han Ling Guo shanshan Guo Jianwei Wang Huai Liu Yanfang Ye Lu Zhang Liting Liu pan Wang Yingqin Li Qingmei He Xiaoqun Yang Qingnan Tang Yang Li YuJing liang XueSong Sun Chuanmiao Xie Yunxian Mo Ying Guo Rui Sun Haoyuan Mo Kajia Cao Xiang Guo Musheng Zeng Haiqiang Mai Jun Ma State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-sen University Cancer CenterGuangzhou 510060P.R.China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer CenterGuangzhou 510060P.R.China Department of Ultrasound Sun Yat-sen University Cancer CenterGuangzhou 510060P.R.China Department of Radiation Oncology Key Laboratory of Translational Radiation OncologyHunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityChangsha 410013P.R.China Department of Science and Education Sun Yat-sen Memorial HospitalGuangzhou 510120P.R.China Department of Radiation Oncology The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510120P.R.China Department of Imaging Sun Yat-sen University Cancer CenterGuangzhou 510060P.R.China Department of Clinical Trial Center Sun Yat-sen University Cancer CenterGuangzhou 510060P.R.China Department of Radiation Oncology Sun Yat-sen University Cancer CenterGuangzhou 510060P.R.China

Background:Famitinib is a tyrosine kinase inhibitor against multiple targets,including vascular endothelial growth factor receptor 2/3,platelet-derived growth factor receptor,and stem cell factor receptor(c-kit).Previous studies have demonstrated anti-tumour activities of famitinib against a wide variety of advanced-stage solid *** aimed to determine the safety and efficacy of famitinib with concurrent chemoradiotherapy(CCRT)in patients with locoregionally advanced nasopharyngeal carcinoma(NPC).We also evaluated the feasibility of contrast-enhanced ultrasound(D-CEUS)as a predictor of early tumour response to famitinib and to correlate functional parameters with clinical ***:The trial was conducted in subjects with stage III or IVa-b NPC using a 3+3 design of escalating fami-tinib ***,subjects received 2 weeks of famitinib monotherapy followed by 7 weeks of famitinib plus CCRT.D-CEUS of the neck lymph nodes was performed at day 0,8 and 15 after famitinib was administered before starting concurrent *** points included safety,tolerability and anti-tumour ***:Twenty patients were enrolled(six each for 12.5,16.5 and 20 mg and two for 25 mg).Two patients in the 25 mg cohort developed dose-limiting toxicities,including grade 4 thrombocytopenia and grade 3 *** most common grade 3/4 adverse events were leukopenia,neutropenia and radiation mucositis.D-CEUS tests showed that more than 60%of patients achieved a perfusion parameter response after 2 weeks taking famitinib alone,and the parameter response was associated with disease *** the famitinib monotherapy stage,three patients(15%)showed partial *** complete response rate was 65%at the completion of treatment and 95%3 months after the treatment *** a median follow-up of 44 months,the 3-year progression-free survival(PFS)and distant metastasis-free survival were 70%and 75%,*** with a decrease of perfusion p

关键词： Nasopharyngeal carcinoma Famitinib Concurrent chemoradiotherapy Phase I dynamic contrast-enhanced ultrasound

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

北部湾钦江响应极端天气的水沙变化过程

引用

热带地理 2023年第11期43卷 2135-2145页

作者：许珊珊杨夏玲黎树式潘嘉嘉梁喜幸南宁师范大学地理科学与规划学院南宁530001 广西北部湾海洋环境变化与灾害研究重点实验室广西钦州535011 广西沿海水文中心广西钦州535000 华东师范大学河口海岸学国家重点实验室上海200241

文章基于近60年来的钦江水沙数据,通过变差系数和贡献率计算法分析钦江在极端天气下的水沙变化。结果表明:1)热带气旋年正常天气的平均流量为32 m^(3)/s,热带气旋过境的平均流量为290 m^(3)/s,是正常天气的9.06倍;热带气旋年的正常天气... 详细信息

文章基于近60年来的钦江水沙数据,通过变差系数和贡献率计算法分析钦江在极端天气下的水沙变化。结果表明:1)热带气旋年正常天气的平均流量为32 m^(3)/s,热带气旋过境的平均流量为290 m^(3)/s,是正常天气的9.06倍;热带气旋年的正常天气的平均输沙量是0.05×10^(4)t,热带气旋过境期间的平均输沙量为1.15×10^(4)t,是正常天气期间的23倍。2)洪水年正常天气的平均流量在375 m^(3)/s,而洪水期平均流量在2725 m^(3)/s左右,是正常天气平均流量的7.27倍。洪水年的正常天气的平均输沙量为0.07×10^(4) t,而洪水期的平均输沙量为1.14×10^(4)t,是正常天气输沙量的16.28倍。3)热带气旋对钦江的径流量平均多年贡献率为10.75%,输沙量贡献率为20.95%;洪水对钦江的径流量平均多年贡献率为16.75%,输沙量贡献率为30.07%。极端天气对钦江的水沙变化具有重要贡献,极端天气发生期间钦江具有“水沙激增”的特征,且极端天气对钦江水沙的影响具有年际变化特点,其热带气旋过境时径流量与输沙量的峰值有逐渐减小趋势,受热带气旋活动影响的减小可能是其中的原因之一。

关键词：极端天气水沙变化钦江北部湾

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

哺乳母猪精准饲喂下料控制系统的设计与试验

引用

农业工程学报 2017年第20期33卷 177-182页

作者：熊本海杨亮郑姗姗曹沛潘晓花吴郭涛中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室北京100193 河南南商农牧科技股份有限公司郑州453500

为满足哺乳母猪获得最大采食量并达到精准饲喂控制等需求,以哺乳母猪为试验对象,设计了一种新型哺乳母猪精准下料控制系统。研究通过控制电动杆的推杆速率、输入电压及电源功率的协同工作,以获得稳定的下料量;采用预设的个性化的采食量... 详细信息

为满足哺乳母猪获得最大采食量并达到精准饲喂控制等需求,以哺乳母猪为试验对象,设计了一种新型哺乳母猪精准下料控制系统。研究通过控制电动杆的推杆速率、输入电压及电源功率的协同工作,以获得稳定的下料量;采用预设的个性化的采食量模型与变容积的精确控制技术,实现对预设饲喂量的准确投喂。系统设计了下料控制系统,主要由定量仓体、电动推杆、堵料上球及堵料下球等部件及嵌入式控制系统组成。试验结果表明,1)当电源功率为150 W时,推杆启动时电压变化略小,对单机下料的精准性影响较小,且当电动推杆速率为60 mm/s,输入电压为11.5 V,下料效果最好(P<0.001),变异系数(CV=3.526%)最小;2)预设的采食量曲线接近哺乳母猪的采食规律,且采食量曲线收敛于对数曲线;3)智能饲喂系统采用4次/d的饲喂频率及与采食曲线的协同工作,与人工饲喂对比,能进一步促进采食量,且采用变化的饲喂时间间隔(06:00,10:00,15:00及22:00),并在不同时间点的饲喂采食量比例分别为30%、20%、20%、30%时,总的采食量最大。综上,该文设计低成本的哺乳母猪精准下料控制系统,采用基于电动推杆的控制机构与嵌入式系统的协同工作,设备控制简单,下料稳定,计量准确,与进口设备及以螺旋输送原理为基础的过往系统比较,成本具有明显优势,适合在中国大、中、小型的种猪场的哺乳车间推广应用。

关键词：控制系统设计嵌入式系统哺乳母猪电动推杆采食量

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

冻融胚胎移植中辅助孵化对妊娠结局及安全性的影响

引用

中华生殖与避孕杂志 2017年第9期37卷 693-697页

作者：赵美吴海霞陈智勤潘家坪梁珊珊黄媚媛滕晓明同济大学附属第一妇婴保健院生殖医学中心上海200040

目的探讨在玻璃化冻融胚胎移植(FET)中,激光辅助孵化(AH)操作后对妊娠结局及母儿安全性的影响。方法回顾性分析2014年1月—2015年10月接受体外受精-胚胎移植(IVF-ET)失败后行FET患者的临床资料,根据患者年龄进行分组后比较AH操作后妊娠... 详细信息

目的探讨在玻璃化冻融胚胎移植(FET)中,激光辅助孵化(AH)操作后对妊娠结局及母儿安全性的影响。方法回顾性分析2014年1月—2015年10月接受体外受精-胚胎移植(IVF-ET)失败后行FET患者的临床资料,根据患者年龄进行分组后比较AH操作后妊娠结局及母儿并发症的发生情况。结果在正常年龄组(0.05);分娩周期中,AH组与对照组早产率、死胎率、母体并发症、新生儿男女比例、胎龄、出生体质量、新生儿缺陷率等均无统计学差异(P<0.05)。结论对于正常年龄患者,卵裂期FET时不建议行AH操作;对于高龄患者,AH操作可提高胚胎种植率及临床妊娠率,但不能明显改善其妊娠结局;AH对母儿安全暂无不良影响。

关键词：辅助生殖技术(ART) 辅助孵化(AH) 冻融胚胎移植(FET)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

覆膜棉田蒸散发日变化规律及其影响因子分析

引用

安全与环境工程 2018年第5期25卷 1-8页

作者：潘杉杉梁杏刘延锋卢昱含李严中国地质大学(武汉)环境学院湖北武汉430074 中国地质大学(武汉)盆地水文过程与湿地生态恢复学术创新基地湖北武汉430074

田间蒸散发是西北干旱地区水文循环的重要组成部分,精确监测田间蒸散发量对该地区农田水量平衡计算和水资源评价具有重要的意义。为探究干旱地区棉田蒸散发量的动态变化规律及其影响因素,在新疆生产建设兵团炮台土壤改良试验站,利用LCp... 详细信息

田间蒸散发是西北干旱地区水文循环的重要组成部分,精确监测田间蒸散发量对该地区农田水量平衡计算和水资源评价具有重要的意义。为探究干旱地区棉田蒸散发量的动态变化规律及其影响因素,在新疆生产建设兵团炮台土壤改良试验站,利用LCpro+光合作用仪测量了膜下滴灌棉田田间蒸散发量。结果表明:棉花叶片蒸腾速率和膜间土面蒸发速率日内均呈现中午高、早晚低的单峰变化规律;棉花铃期到吐絮期期间棉花叶片日均蒸腾量为2.20mm,膜间土面日均蒸发量为0.16mm,单膜下棉田日均蒸散发量为2.21mm,蒸腾是土壤水分散失的主要方式;受灌水、气象等因素的影响,土壤温度和土壤含水率均呈现波动性变化;棉花叶片蒸腾速率和膜间土面蒸发速率与土壤温度在日内按正弦曲线变化,膜间土面蒸发速率与土壤温度的相关性较棉花叶片蒸腾速率更为显著;膜间土面蒸发量与土壤含水率的相关性也较棉花叶片蒸腾量显著。

关键词：覆膜棉田田间蒸散发棉花叶片蒸腾膜间土面蒸发动态变化影响因素

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：