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Removal of pathogenic indicator microorganisms during partial nitrification:the role of free nitrous acid

Frontiers of Environmental Science & Engineering 2024年第3期18卷 93-102页

作者： jiaojiao xu Xiaotian Chen Rui Tang Jingwei Feng Shoujun Yuan Wei Wang Zhen-Hu Hu Anhui Engineering Laboratory of Rural Water Environment and Resource School of Civil EngineeringHefei University of TechnologyHefei 230009China Hefei 168 High School Hefei 230025China Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power Shanghai University of Electric PowerShanghai 200090China

Digested wastewater contains pathogenic microorganisms and high ammonia concentrations,which can pose a potential risk to public *** removal of pathogens and nitrogen is crucial for the post-treatment of digested *** nitrification-anammox is an energy-saving nitrogen removal *** nitrous acid(FNA),an intermediate product of partial nitrification,has the potential to inactivate ***,the efficiency and mechanisms of FNA-related inactivation in pathogens during partial nitrification remains *** this study,Enterococcus and Escherichia coli(***)were selected to investigate the efficiency and mechanisms of FNA-related inactivation in partial nitrification *** results revealed that 83%±13%and 59%±27%of *** and Enterococcus were removed,respectively,in partial nitrification process at FNA concentrations of 0.023−0.028 mg/*** the concentration of FNA increased from 0 to 0.5 mg/L,the inactivation efficiencies of *** and Enterococcus increased from 0 to 99.9%and 89.9%,*** exhibited a higher resistance to FNA attack compared to ***.3D-laser scanning microscopy(3D-LSM)and scanning electron microscopy(SEM)revealed that FNA exposure caused the surface collapse of *** and Enterococcus,as well as visible pore formation on the surface of *** cells.4',6-Diamidino-2-phenylindole dihydrochloride n-hydrate(DAPI)/propidium iodide(PI)and biomolecule leakage confirmed that inactivation of *** and Enterococcus occurred due to breakdown of cell walls and cell *** findings indicate that partial nitrification process can be used for the removal of residual pathogenic microorganisms.

关键词： Partial nitrification Free nitrous acid Pathogenic indicator microorganism Inactivation Cell structure

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Spatiotemporal Distributions of Bacterioplankton Communities in the Qiantang River (Hangzhou Section), China

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JOURNAL OF OCEAN UNIVERSITY OF CHINA 2024年第4期23卷 1102-1114页

作者： xu, jiaojiao Zhao, Li Lukwambe, Betina Nicholaus, Regan Zhu, Jinyong Yang, Wen Zheng, Zhongming Ningbo Univ Sch Marine Sci Ningbo 315211 Peoples R China Univ Dar Es Salaam Sch Aquat Sci & Fisheries Technol Dar Es Salaam Tanzania Mbeya Univ Sci & Technol Dept Nat Sci Mbeya Tanzania

The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic ecosystems. In this study, we conducted a comprehensive investigation of the bacterioplankton communities in the Qiantang River (Hangzhou section). Water samples were collected quarterly from seven sites over a one-year period. Physical and chemical parameters, including dissolved oxygen (DO), water temperature (WT), chemical oxygen demand (COD), nitrite (NO2-), active phosphate (PO43-) and other indices were determined. In this study, the alpha diversity, beta diversity and abundance differences of bacterial communities were investigated using 16S rRNA high-throughput sequencing analysis. The spatial and temporal distribution characteristics and main driving factors of the bacterioplankton community structure and diversity were discussed. The results showed that a total of 57 phyla were detected in the bacterioplankton community, among which Proteobacteria and Actinomycetes were the two dominant groups with the highest relative abundance. The results of PCoA based on Bray-Curtis distance showed that the sampling season had a slightly greater effect on the changes in bacterioplankton community structure in the Qiantang River. Mantel and partial Mantel test showed that environmental variables (Mantel r = 0.6739, P < 0.0001; partial Mantel r = 0.507, P = 0.0001) were more important than geographical distance (Mantel r = 0.5322, P < 0.001; partial Mantel r = 0.1563, P = 0.001). The distance attenuation model showed that there was significant distance attenuation in all four seasons, and the maximum limit of bacterial community diffusion was found in spring. RDA analysis showed that nine environmental factors, including COD, WT and DO, significantly affected community distribution (P < 0.05). This study provides valuable insights into the spatial and temporal distribution characteristics of bacterioplankton communities,

关键词： Qiantang River bacterioplankton community spatial and temporal distribution 16S rRNA range attenuation MAJOR BACTERIAL GROUPS SEASONAL DYNAMICS PATTERNS WATER BIOGEOGRAPHY SPP.

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极薄IF钢的连续退火工艺研究

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四川冶金 2024年第3期46卷 12-16页

作者：许姣姣宝钢钢铁股份有限公司中央研究院江苏南京210039

在连续退火模拟装置中进行极薄料的模拟退火处理,再通过硬度试验、拉伸试验和金相组织观察,研究不同成分体系IF钢的再结晶温度差异,以及在不同退火温度下的性能变化规律。结果表明,同样压缩比条件下,Mn IF钢先于P IF钢开始再结晶,但两... 详细信息

在连续退火模拟装置中进行极薄料的模拟退火处理,再通过硬度试验、拉伸试验和金相组织观察,研究不同成分体系IF钢的再结晶温度差异,以及在不同退火温度下的性能变化规律。结果表明,同样压缩比条件下,Mn IF钢先于P IF钢开始再结晶,但两者再结晶的过程及速率基本一致;Mn IF钢700℃时完成再结晶,P IF钢720℃时完成再结晶;两种IF钢完全再结晶后达到的力学性能相当,实际生产时可根据生产组织需要选择合适的成分设计。

关键词： IF钢连续退火再结晶温度力学性能镀锡板

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液相色谱-串联质谱-内标法测定尿液中河豚毒素

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卫生研究 2024年第1期53卷 95-101页

作者：徐小民蔡增轩许娇娇郑熠斌徐美佳韩见龙浙江省疾病预防控制中心杭州310051

目的建立液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)-内标法测定尿液中河豚毒素(tetrodotoxin,TTX)的分析方法。方法样品中的TTX经乙酸/甲醇/乙腈(0.005 mL/0.8 mL/1.8 mL)混合溶剂提取,阳离子交换固相萃取(solid phase extraction,SPE)小柱净化,采用含0.3%盐酸的50%乙腈/水洗脱、氨水中和后用XBridgeTMBEH Amide柱(150 mm×3.0 mm,1.7μm)分离,MS/MS测定。通过优化样品提取和SPE净化条件,解决阳离子交换SPE小柱净化尿液基质中TTX回收率偏低、质谱检测基质抑制效应强的难题。结果以春雷霉素为内标定量测定,TTX在0.2~200μg/L范围内线性关系良好,相关系数(r2)0.997,样品基质和纯溶剂中TTX线性相关方程的斜率比为0.95;尿液中TTX检出限和定量限分别为0.1和0.2μg/L;三水平加标(0.2、10.0和200μg/L)平均回收率为89.3%~95.3%,相对标准偏差≤5.1%(n=6)。11例中毒患者尿样中TTX含量为0.4~138μg/L;中毒3天以内获得的尿液中TTX的检出率为100%。结论该方法操作简单、准确、灵敏度高,可以为TTX中毒事件快速处置和毒素体内代谢研究提供可靠的技术支撑。

关键词：液相色谱-串联质谱法固相萃取河豚毒素尿液食物中毒

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A rationally designed cancer vaccine based on NIR-II fluorescence image-guided light-triggered remote control of antigen cross-presentation and autophagy

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药学学报:英文版 2023年第7期13卷 3121-3136页

作者： Aihua Wu Afeng Yang Qinli Tong Guoguang Wei Sihang Zhang Sheng Yu Chen Zhang jiaojiao xu Wei Lu School of Pharmacy&Minhang Hospital Key Laboratory of Smart Drug DeliveryMinistry of Education&State Key Laboratory of Molecular Engineering of PolymersFudan UniversityShanghai 201203China

Cancer vaccines represent a promising immunotherapeutic treatment *** promotion of cross-presentation of extracellular tumor-associated antigens on the major histocompatibility complex(MHC) class I molecules and dendritic cell maturation at the appropriate time and place is crucial for cancer vaccines to prime cytolytic T cell response with reduced side *** vaccination strategies,however,are not able to achieve the spatiotemporal control of antigen ***,we report a liposomal vaccine loading the second near-infrared window(NIR-II,1000—1700 nm) fluorophore BPBBT with an efficient photothermal conversion effect that offers an NIR-light-triggered endolysosomal escape under the imaging *** NIR-II image-guided vaccination strategy specifically controls the cytosolic delivery of antigens for cross-presentation in the draining lymph nodes(DLNs).Moreover,the photothermally induced endolysosomal rupture initiates *** also find that the adjuvant simvastatin acts as an autophagy activator through inhibiting the PI3K/AKT/m TOR *** light-induced autophagy in the DLNs together with simvastatin treatment cooperatively increase MHC class II expression by activating autophagy machinery for dendritic cell *** study presents a paradigm of NIR-II image-guided light-triggered *** approach for remote control of antigen cross-presentation and autophagy represents a new strategy for vaccine development.

关键词： Vaccine Light-triggered NIR-II imaging Cross-presentation Autophagy Simvastatin Endolysosomal escape Dendritic cell maturation

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KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer

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Acta Pharmaceutica Sinica B 2023年第10期13卷 4217-4233页

作者： Cancan Zheng Xiaomei Yu Taoyang xu Zhichao Liu Zhili Jiang jiaojiao xu Jing Yang Guogeng Zhang Yan He Han Yang Xingyuan Shi Zhigang Li Jinbao Liu Wen Wen xu Key Laboratory of Biological Targeting Diagnosis Therapy and Rehabilitation of Guangdong Higher Education Institutesthe Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510230China Department of Thoracic Surgery Shanghai Chest HospitalSchool of MedicineShanghai Jiao Tong University.Shanghai 200030China Department of Radiation Oncology the Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510230China Department of Thoracic Surgery Sun Yat-sen University Cancer CenterGuangzhou 510060China Guangdong Provincial Key Laboratory of Protein Modification and Degradation State Key Laboratory of Respiratory Diseaseand School of Basic Medical SciencesGuangzhou Medical UniversityGuangzhou 511495China

Increasing evidences suggest the important role of calcium homeostasis in hallmarks of cancer,but its function and regulatory network in metastasis remain unclear.A comprehensive investigation of key regulators in cancer metastasis is urgently *** sequencing(RNA-seq)of primary esophageal squamous cell carcinoma(ESCC)and matched metastatic tissues and a series of gain/loss-of-function experiments identified potassium channel tetramerization domain containing 4(KCTD4)as a driver of cancer ***4 expression was found upregulated in metastatic *** KCTD4 expression is associated with poor prognosis in patients with ESCC and contributes to cancer metastasis in vitro and in ***,KCTD4 binds to CLIC1 and disrupts its dimerization,thus increasing intracellular Ca^(2+)level to enhance NFATc1-dependent fibronectin ***4-induced fibronectin secretion activates fibroblasts in a paracrine manner,which in turn promotes cancer cell invasion via MMP24 signaling as positive ***,a lead compound K279-0738 significantly suppresses cancer metastasis by targeting the KCTD4-CLIC1 interaction,providing a potential therapeutic *** together,our study not only uncovers KCTD4 as a regulator of calcium homeostasis,but also reveals KCTD4/CLIC1-Ca^(2+)-NFATc1-fibronectin signaling as a novel mechanism of cancer *** findings validate KCTD4 as a potential prognostic biomarker and therapeutic target for ESCC.

关键词： Cancermetastasis Calcium homeostasis NFAT signaling Fibroblasts Esophageal cancer

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Causal temporal graph attention network for fault diagnosis of chemical processes

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Chinese Journal of Chemical Engineering 2024年第6期70卷 20-32页

作者： jiaojiao Luo Zhehao Jin Heping Jin Qian Li xu Ji Yiyang Dai School of Chemical Engineering Sichuan UniversityChengdu 610065China China Three Gorges Corporation Beijing 100038China

Fault detection and diagnosis(FDD)plays a significant role in ensuring the safety and stability of chemical *** the development of artificial intelligence(AI)and big data technologies,data-driven approaches with excellent performance are widely used for FDD in chemical ***,improved predictive accuracy has often been achieved through increased model complexity,which turns models into black-box methods and causes uncertainty regarding their *** this study,a causal temporal graph attention network(CTGAN)is proposed for fault diagnosis of chemical processes.A chemical causal graph is built by causal inference to represent the propagation path of *** attention mechanism and chemical causal graph were combined to help us notice the key variables relating to fault *** in the Tennessee Eastman(TE)process and the green ammonia(GA)process showed that CTGAN achieved high performance and good explainability.

关键词： Chemical processes Safety Fault diagnosis Causal discovery Attention mechanism Explainability

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Enhanced photocatalytic ammonia oxidation activity and nitrogen selectivity over Ag/AgCl/N-TiO_(2) photocatalyst

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Journal of Environmental Sciences 2024年第4期138卷 395-405页

作者： jiaojiao Guan Daorong Li Jiahui Feng Peng xu Zhaonian Li Siqi Ge Hongxia Chen Kunfeng Zhang College of Environmental Engineering Henan University of TechnologyZhengzhou 450001China CAS Key Laboratory of Standardization and Measurement for Nanotechnology National Center for Nanoscience and TechnologyBeijing 100190China Department of Chemistry Tsinghua UniversityBeijing 100084China

The removal of ammonia(NH_(3))emitted from agricultural and industrial activities is of great significance to protect human health and ecological *** NH_(3)oxidation to N2under mild conditions is a promising ***,developing visible light photocatalysts for NH_(3)oxidation is still in its ***,we fabricate N-TiO_(2)and Ag/AgCl/N-TiO_(2)photocatalysts by sol-gel and photodeposition methods,*** introduction of N not only endows TiO_(2)with visible light response(absorption edge at460 nm)but also results in the formation of heterophase junction(anatase and rutile).Thus,N-TiO_(2)shows 2.0 and 1.8 times higher than those over anatase TiO_(2)and commercial TiO_(2)for NH_(3)oxidation under full spectrum ***,surface modification of Ag can simultaneously enhance visible light absorption(generating localized surface plasmon resonance effect)and charge separation ***,the photocatalytic activity of Ag/AgCl/N-TiO_(2)is further ***,the presence of N and Ag also enhances the selectivity of N2product owing to the change of reaction *** work simultaneously regulates photocatalytic conversion efficiency and product selectivity,providing some guidance for developing highly efficient photocatalysts for NH_(3)elimination.

关键词： TiO_(2) Doping Surface modification Photocatalysis NH_(3)elimination

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中国茶树植物新品种保护现状与分析

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中国种业 2024年第6期 1-6,13页

作者：胡梦芹邓伟刘振田建华龙代彬李乐赵佳许姣姣许晓庆彭云湘西土家族苗族自治州农业科学研究院吉首416000 农业农村部科技发展中心北京102600 湖南省农业科学院茶叶研究所长沙410000

植物新品种保护是维护植物品种权合法权益、促进育种创新的根本保障。自植物新品种保护制度实施到2023年底,我国茶树的品种权申请量为529件,授权量为142件。通过分析申请主体、申请年份和申请地区的申请量和授权量,总结出茶树品种保护... 详细信息

植物新品种保护是维护植物品种权合法权益、促进育种创新的根本保障。自植物新品种保护制度实施到2023年底,我国茶树的品种权申请量为529件,授权量为142件。通过分析申请主体、申请年份和申请地区的申请量和授权量,总结出茶树品种保护申请量和授权量具有波浪式增长、省份之间申请量差异大、申请主体以科研单位为主等几大特点,针对这些特点归纳出存在的问题并提出工作建议,以期为该领域有关人员提供参考。

关键词：茶树植物新品种权申请量授权量保护

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肺癌患者肌少症的影响因素及干预现状

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中国医学创新 2024年第12期21卷 183-188页

作者：韩笑朱思曼张珺艾琼琼徐娇娇易思奇马海萍南昌大学护理学院江西南昌330006 江西科技学院医学院江西南昌330098 南昌大学第二附属医院胸外科江西南昌330006

对肺癌与肌少症之间的关系、肺癌患者肌少症的护理筛查与评估方法、影响因素及护理干预措施进行综述。旨在提高临床护理人员对肺癌患者合并肌少症的认识,同时为肺癌合并肌少症患者制订精准干预方案及实施个体化管理提供思路和参考。

关键词：肺癌肌少症护理评估护理干预

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