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Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals

Infectious Diseases of Poverty 2017年第1期6卷 754-760页

作者： Xin Zhang Chuan-Chuan He Jin-Ming Liu Hao Li Ke Lu Zhi-Qiang Fu Chuan-Gang Zhu Yi-Ping Liu Lai-Bao Tong De-bao Zhou Li Zha Yang Hong Ya-Mei Jin jiao-jiao lin Shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Animal ParasitologyMinistry of AgricultureShanghai 200241People’s Republic of China Anhui Center for Animal Disease Control and Prevention HefeiPeople’s Republic of China Wangjiang county Center for Animal Husbandry and Veterinary Bureau AnqingPeople’s Republic of China Dongzhi county Center for Animal Husbandry and Veterinary Bureau ChizhouPeople’s Republic of China Huangshan Center for Animal Disease Control and Prevention Huangshan 341000People’s Republic of China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious and Zoonosea YangzhouJiangsu province 225009People’s Republic of China

Background:Schistosomiasis japonica is a common *** animals are the primary source of infection and play an important role in disease *** prevalence and infectivity of this disease in domestic animals in China have significantly decreased and,for this reason,diagnostics with a higher sensitivity have become increasingly *** was reported that polymerase chain reaction(PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and *** present study aimed to develop a PCR-based method for detection of Schistosoma japonicum infection in domestic ***:A specific nested-PCR assay was developed to detect *** infection in domestic animals via amplification of a 231-bp DNA fragment of retrotransposon *** developed assay was first used in sera and dry blood filter paper(DBFP)from goats and buffaloes at different time points of ***,78 DBFPs from 39 artificially-infected bovines at 14 and 28 days post-infection and 42 DBFPs from schistosome-negative bovines from the city of Huangshan in the Anhui province were used to evaluate the diagnostic ***,this assay was used to detect *** infection in domestic animals in Dongzhi and Wangjiang ***:The expected PCR product was detected in eggs and adult worms of *** and blood samples from ***-infected goats and water buffaloes,but not from Fasciola and Haemonchus contortus *** nested-PCR assay could detect the target *** DNA in DBFPs from goats and buffaloes after day 3 *** sensitivity in buffaloes at 14 and 28 days post-infection was 92.30%(36/39)and 100%(39/39),*** specificity was 97.60%(41/42).The positivity rates in Dongzhi and Wangjiang counties were 6.00%and 8.00%in bovines and 22.00%and 16.67%in goats,*** positivity rates in goats in both counties were higher than those in bovines wit

关键词： Schistosomiasis Domestic animals Nested-PCR Diagnosis

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Advances in the Development of Gradient Scaffolds Made of Nano-Micromaterials for Musculoskeletal Tissue Regeneration

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NANO-MICRO LETTERS 2025年第1期17卷 1-46页

作者： Fang, Lei lin, Xiaoqi Xu, Ruian Liu, Lu Zhang, Yu Tian, Feng Li, jiao jiao Xue, Jiajia Beijing Univ Chem Technol Beijing Lab Biomed Mat Beijing 100029 Peoples R China Beijing Univ Chem Technol State Key Lab Organ Inorgan Composites Beijing 100029 Peoples R China Univ Technol Sydney Fac Engn & IT Sch Biomed Engn Sydney NSW 2007 Australia

This review highlights the gradient variations in the structural composition of musculoskeletal tissues and comprehensively examines recent progress in the fabrication and application of biomimetic gradient scaffolds for musculoskeletal *** challenges and prospects of gradient scaffolds for clinical application are discussed. The intricate hierarchical structure of musculoskeletal tissues, including bone and interface tissues, necessitates the use of complex scaffold designs and material structures to serve as tissue-engineered substitutes. This has led to growing interest in the development of gradient bone scaffolds with hierarchical structures mimicking the extracellular matrix of native tissues to achieve improved therapeutic outcomes. Building on the anatomical characteristics of bone and interfacial tissues, this review provides a summary of current strategies used to design and fabricate biomimetic gradient scaffolds for repairing musculoskeletal tissues, specifically focusing on methods used to construct compositional and structural gradients within the scaffolds. The latest applications of gradient scaffolds for the regeneration of bone, osteochondral, and tendon-to-bone interfaces are presented. Furthermore, the current progress of testing gradient scaffolds in physiologically relevant animal models of skeletal repair is discussed, as well as the challenges and prospects of moving these scaffolds into clinical application for treating musculoskeletal injuries.

关键词： Gradient scaffolds Musculoskeletal tissues Advanced manufacturing Biomaterials Tissue regeneration

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Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome(Schistosomajaponicum)by RNA Interference

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 386-390页

作者： Guo-Feng CHENG~1 jiao-jiao lin~1 Yi SHI~2 You-Xin JIN~2 Zhi-Qiang FU~1 Ya-Mei JIN~1 Yuan-Cong ZHOU~2 You-Min CA~1 1 Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Key Lahoratory of Animal Parasitology,Ministry of Agriculture, Shanghai 200232, China 2 State Key Laboratory, of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. Shanghai 200031, China Shanghai Institute of Animal Parasitology Chinese Academy of Agricultural Sciences Key Laboratory of Animal Parasitology Ministry of Agriculture Shanghai 200232 China.

The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary forthe pairing between the male and female worms is specifically expressed in the adult male *** is widely distributed in the adult female worm after *** transcription-polymerase chainreaction(RT-PCR)and immunofluorescence were employed to analyze the relationship between the RNAieffect and dsRNA dosage in the *** results revealed that the inhibition of SjGCP expression bysiRNA is ***-PCR analysis showed that the SjGCP transcript level was reduced by 75%when 100 nM dsRNA was applied.

关键词： RNA interference gynecophoral canal protein gene Schistosoma japonicum dosage

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Mechanical properties and friction behaviors of CNT/AlSi_(10)Mg composites produced by spark plasma sintering

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International Journal of Minerals,Metallurgy and Materials 2017年第5期24卷 584-593页

作者： lin-zhi Wang Ying Liu jiao-jiao Wu Xi Zhang Chongqing Key Laboratory of Additive Manufacturing Technology and Systems Chongqing Institute of Green and Intelligent TechnologyChinese Academy of Sciences College of Material Science and Engineering Sichuan University College of Material Science and Engineering Southwest Jiaotong university

The mechanical properties and friction behaviors of CNT/AlSi10Mg composites produced by spark plasma sintering (SPS) were investigated. The results showed that the densities of the sintered composites gradually increased with increasing sintering temperature and that the highest microhardness and compressive strength were achieved in the specimen sintered at 450A degrees C. CNTs dispersed uniformly in the Al-Si10Mg matrix when the addition of CNTs was less than 1.5wt%. However, when the addition of CNTs exceeded 1.5wt%, the aggregation of CNTs was clearly observed. Moreover, the mechanical properties (including the densities, compressive strength, and microhardness) of the composites changed with CNT content and reached a maximum value when the CNT content was 1.5wt%. Meanwhile, the minimum average friction coefficient and wear rate of the CNT/AlSi10Mg composites were obtained with 1.0wt% CNTs.

关键词： metal matrix composites carbon nanotubes (CNTs) aluminum silicon magnesium alloys spark plasma sintering mechanical properties friction

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Machine learning driven rational design of dual atom catalysts on graphene for carbon dioxide electroreduction

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Nano Research 2025年第1期18卷 186-194页

作者： Dongxu jiao Xinyi Li Mingzi Sun lin Liu Jinchang Fan Jingxiang Zhao Bolong Huang Xiaoqiang Cui State Key Laboratory of Automotive Simulation and Control School of Materials Science and Engineeringand Key Laboratory of Automobile Materials of MOEJilin UniversityChangchun 130012China Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina College of Chemistry and Chemical Engineering and Key Laboratory of Photonic and Electronic Bandgap MaterialsMinistry of EducationHarbin Normal UniversityHarbin 150025China

The development of high-performance atomiccatalysts for the carbon dioxide reduction reaction(CO_(2)RR)is atime-consuming process due to the complexity of the reactionmechanism and the uncertainty of the active ***,wehave proposed combining density functional theory(DFT)andmachine learning(ML)to investigate the potential of topologicalgraphene-based dual-atom catalysts(DACs)as CO_(2)*** analyzing the ML results,we identify thenumber of d-orbital electrons in the active site as a key factorinfluencing the CO_(2)RR catalytic ***,wepropose a simple descriptor to measure the CO_(2)RR activity ofthese *** findings provide plausible explanations for thesynergistic interactions between bimetallic atoms in CO_(2)RR andallow us to screen the homogeneous Ni-Ni pair as the mostpromising dual-atom *** work offers a fast MLapproach based on limited DFT calculations to predict the mostelectroactive and stable DACs on carbon support for CO_(2)RR,facilitating rapid screening of high-performance dual-atomcatalysts.

关键词： CO_(2)reaction reduction dual-atom catalysts density functional theory machine learning

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Stream cipher designs: a review

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Science China(Information Sciences) 2020年第3期63卷 80-104页

作者： lin jiao Yonglin HAO Dengguo FENG State Key Laboratory of Cryptology State Key Laboratory of Computer Science Institute of Software Chinese Academy of Sciences

Stream cipher is an important branch of symmetric cryptosystems, which takes obvious advantages in speed and scale of hardware implementation. It is suitable for using in the cases of massive data transfer or resource constraints, and has always been a hot and central research topic in cryptography. With the rapid development of network and communication technology, cipher algorithms play more and more crucial role in information security. Simultaneously, the application environment of cipher algorithms is increasingly complex, which challenges the existing cipher algorithms and calls for novel suitable designs. To accommodate new strict requirements and provide systematic scientific basis for future designs, this paper reviews the development history of stream ciphers, classifies and summarizes the design principles of typical stream ciphers in groups, briefly discusses the advantages and weakness of various stream ciphers in terms of security and implementation. Finally, it tries to foresee the prospective design directions of stream ciphers.

关键词： stream cipher survey lightweight authenticated encryption homomorphic encryption

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Effects of mask wearing duration and relative humidity on thermal perception in the summer outdoor built environment

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Building Simulation 2023年第9期16卷 1601-1616页

作者： Rong Hu Jianlin Liu Yongxin Xie jiao jiao Zhaosong Fang Borong lin College of Environmental Science and Engineering Donghua UniversityShanghaiChina Department of Building Science School of ArchitectureTsinghua UniversityBeijingChina School of Civil Engineering Guangzhou UniversityGuangzhouChina Department of Building Environment and Energy Engineering The Hong Kong Polytechnic UniversityKowloonHong KongChina

During the pandemic,face masks are one of the most significant self-protection necessities,but they also cause heat *** using the ERA5(ECMWF Reanalysis 5th Generation)database and the local weather bureau data,the effect of mask wearing on outdoor thermal sensation has been investigated by a survey conducted in the hot summer and cold winter region of eastern China in the summer of *** show that wearing a face mask for a longer period result in a higher level of discomfort,and the primary source of discomfort is hot and stuffy *** effect of relative humidity is crucial for mask wearers in warm-biased thermal environments,as mean thermal sensation vote(TSV)peaks when environmental relative humidity reaches the range of 70%to 80%and decreases after this range due to the evaporation within the microclimate created by a face ***,prolonged mask wearing increases participants’hot feelings,especially in warm ***,participants wearing face masks for less than 30 min feel hot at a physiological equivalent temperature(PET)value of 34.4℃,but those who wear them for over 60 min express hot feelings even at a PET value of 24.7℃.The participants who wear a face mask while walking slowly outdoors have similar thermal sensations to those who do not wear a mask,but are in a higher activity *** findings demonstrate that mask wearing has a crucial impact on outdoor thermal comfort assessment in a warm-biased outdoor thermal environment.

关键词： outdoor thermal comfort disposable medical masks mask wearing duration relative humidity physiological equivalent temperature(PET)

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An evaporation duct prediction model coupled with the MM5

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Acta Oceanologica Sinica 2015年第5期34卷 46-50页

作者： jiao lin ZHANG Yonggang Department of Military Oceanography Dalian Naval Academy

Evaporation duct is an abnormal refractive phenomenon in the marine atmosphere boundary layer. It has been generally accepted that the evaporation duct prominently affects the performance of the electronic equipment over the sea because of its wide distribution and frequent occurrence. It has become a research focus of the navies all over the world. At present, the diagnostic models of the evaporation duct are all based on the Monin-Obukhov similarity theory, with only differences in the flux and character scale calculations in the surface layer. These models are applicable to the stationary and uniform open sea areas without considering the alongshore effect. This paper introduces the nonlinear factor a and the gust wind item wg into the Babin model, and thus extends the evaporation duct diagnostic model to the offshore area under extremely low wind speed. In addition, an evaporation duct prediction model is designed and coupled with the fifth generation mesoscale model （MMS）. The tower observational data and radar data at the Pingtan island of Fujian Province on May 25-26, 2002 were used to validate the forecast results. The outputs of the prediction model agree with the observations from 0 to 48 h. The relative error of the predicted evaporation duct height is 19.3% and the prediction results are consistent with the radar detection.

关键词： evaporation duct diagnosis mesoscale model (MM5) evaporation duct prediction model numerical simulation

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Two-Level linear Relaxation Method for Generalized linear Fractional Programming

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Journal of the Operations Research Society of China 2023年第3期11卷 569-594页

作者： Hong-Wei jiao You-lin Shang Postdoctoral Research Base Henan Institute of Science and TechnologyXinxiang453003HenanChina Postdoctoral Research Station of Control Science and Engineering Henan University of Science and TechnologyLuoyang471023HenanChina

This paper presents an efficient algorithm for globally solving a generalized linear fractional programming *** establishing this algorithm,we firstly construct a two-level linear relaxation method,and by utilizing the method,we can convert the initial generalized linear fractional programming problem and its subproblems into a series of linear programming relaxation *** on the branch-and-bound framework and linear programming relaxation problems,a branch-and-bound algorithm is presented for globally solving the generalized linear fractional programming problem,and the computational complexity of the algorithm is ***,numerical experimental results demonstrate the feasibility and efficiency of the proposed algorithm.

关键词： Generalized linear fractional programming Global optimization Two-level linear relaxation method Branch-and-bound

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Modification of Proliferation and Morphology in Human Umbilical Vein Endothelial Cells via in vitro High-Frequency, Low-Energy, linear-Focused,Continuous Ultrasound Stimulation

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Biomedical and Environmental Sciences 2020年第9期33卷 727-730页

作者： SUN An Yu LAI jiao jiao DU Hui lin State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310007ZhejiangChina Polytechnic Institute Zhejiang UniversityHangzhou 310015ZhejiangChina

Since the bioeffects of ultrasound were discovered, its use has become widespread in the field of therapeutics[1]. Two classes of ultrasound bioeffects on tissues and cells are known: thermal and non-thermal[2]. Ultra... 详细信息

关键词： Ultrasound Umbilical Focus

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