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MicroRNA-375 Suppresses the Tumor Aggressive Phenotypes of Clear Cell Renal Cell Carcinomas through Regulating YWHAZ

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Chinese Medical Journal 2018年第16期131卷 1944-1950页

作者： Xiang Zhang Nai-Dong Xing Cheng-Jun Lai Rui Liu wei jiao Jue Wang Jie Song Zhong-Hua Xu Department of Urology Qilu Hospital of Shandong University Jinan Shandong 250012 China Teaching and Research Institute of Urology School of Medicine Shandong University Jinan Shandong 250012 China Department of Urology Qingyun People's Hospital Dezhou Shandong 253700 China Department of the First Operation Room Qilu Hospital of Shandong University Jinan Shandong 250012 China Central Laboratory The Second Hospital of Shandong University Jinan Shandong 250012 China Medicaid and Health Care Service Office Qilu Hospital of Shandong University Jinan Shandong 250012 China

Background： MicroRNAs （miRNAs） are key regulators during tumor initiation and progression. MicroRNA-375 （MiR-375） has been proven to play a tumor-suppressive role in various types of human malignancies; however, its biological role in clear cell renal cell carcinoma （ccRCC） remains unclear. The purpose of this study was to explore the biologic role as well as the underlying mechanism of miR-375 in ccRCC progression. Methods： Quantitative polymerase chain reaction （qPCR） was applied to test the expression of miR-375 in tissues and cell lines by t-test. Functional experiments were used to investigate the biological role of miR-375 utilizing a gain-of-function strategy. The target of miR-375 was investigated by bioinformatic analysis and further verified by luciferase reporter assay, qPCR, Western blotting, and functional experiments in vitro. Results： Our study demonstrated that miR-375 was significantly downregulated in ccRCC tissues （cancer vs. normal, 0.804 ±0.079 vs. 1.784 ± 0.200, t = 5.531 P 〈 0.0001 ） and cell lines, and loss ofmiR-375 expression significantly associated with advanced Fuhrman nuclear grades （Grade Ⅲ and Ⅳ vs. Grade Ⅰ and Ⅱ, 1.000 ± 0.099 vs. 1.731 ± 0.189, t = 3.262 P = 0.003）. Functional studies demonstrated that miR-375 suppressed ccRCC cell proliferation, migration, and invasion （all P 〈 0.05 in both 786-0 and A498 cell lines）. Multiple miRNA target prediction algorithms indicated the well-studied oncogene YWHAZ as a direct target ofmiR-375, which was further confirmed by the luciferase reporter assay, qPCR, and Western blotting. Moreover, restoration of YWHAZ could rescue the antiproliferation effect ofmi R-375. Conclusions： The data provide the solid evidence that miR-375 plays a tumor-suppressive role in ccRCC progression, partially through regulating YWHAZ. This study expands the antitumor profile ofmiR-375, and supports its role as a potential therapeutic target in ccRCC treatment.

关键词： Clear Cell Renal Cell Carcinoma MicroRNA-375 Proliferation YWHAZ

Mapping of qGL7-2, a grain length QTL on chromosome 7 of rice

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Journal of Genetics and Genomics 2010年第8期37卷 523-531页

作者： Gaoneng Shao Shaoqing Tang Ju Luo Guiai jiao Xiangjin wei Ao Tang Jianli Wu Jieyun Zhuang Peisong Hu State Key Laboratory of Rice Biology China National Rice Research lnstitt~te Hangzhou 310006 China

A residual heterozygous line （RHL） carrying a heterozygous segment between two S SR loci RM 11 and RM134 on the rice chromo- some 7 was selected from a set of recombinant inbred lines from the cross D50 （javanica）/HB277 （indica）. The former parent produces much longer grains than the latter. Selfed progenies of this selection were analyzed genotypically （SSRs） and phenotypically （grain length）. Grain length was discontinuously variable in the mapping populations, allowing for the placement of this QTL qGL7-2 within a -4.8 cM interval defined by RM351 and RM234. A set of new markers within this region were developed, which narrowed the QTL to a 278 kb region defined by the markers Indell and RM21945. This region contains 49 predicted genes. The results also suggest that the novel allele for grain length will be used for the application of marker assisted selection for the improvement of grain length.

关键词： grain length mapping quantitative trait locus residual heterozygous line rice

New bisabolane-type phenolic sesquiterpenoids from the marine sponge Plakortis simplex

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Chinese Journal of Natural Medicines 2021年第8期19卷 626-631页

作者： WANG Jie LIU Li HONG Li-Li ZHAN Kai-Xuan LIN Zheng-Jiang jiao wei-Hua LIN Hou-Wen School of Food and Pharmacy Zhejiang Ocean UniversityZhoushan 316000China Research Center for Marine Drugs State Key Laboratory of Oncogenes and Related GenesDepartment of PharmacyRen Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications Liaoning University of Traditional Chinese MedicineShenyang 110847China

Six new bisabolane-type phenolic sesquiterpenoids,including plakordiols A-D(1-4),(7 R,10 R)-hydroxycurcudiol(5)and(7 R,10 S)-hydroxycurcudiol(6)were isolated from the marine sponge Plakortis simplex collected from the South China *** structures were determined based on extensive analysis of spectroscopic *** configurations were assigned by coupling constant analysis,NOESY correlations,and the modified Mosher’s ***,their cytotoxic and antibacterial activities were evaluated.

关键词： Marine sponge Plakortis simplex Sesquiterpene phenols Plakordiols

Vaginal enterocele after cystectomy:A case report

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World Journal of Clinical Cases 2022年第6期10卷 2045-2052页

作者： Shuai-Hong Liu Yu-Hao Zhang Hai-Tao Niu Dong-Xu Tian Fei Qin wei jiao Department of Urology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina Department of Clinical Medicine Qingdao UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND After undergoing radical cystectomy combined with hysterectomy,female patients may suffer from pelvic organ prolapse due to the destruction of pelvic structures,which mainly manifests as the prolapse of tissues of the vulva to varying degrees and can be accompanied by symptoms,such as bleeding and *** this complication is present,surgical intervention is needed to resolve ***,preventing and managing this complication is especially *** SUMMARY The postoperative occurrence of acute enterocele is rare,and a case of acute small bowel vaginosis 2 mo after radical cystectomy with hysterectomy is *** the patient was admitted,physical examination revealed that the small bowel was displaced approximately 20 cm because of vaginocele.A team of gynecological,general surgery,and urological surgeons was employed to return the small bowel and repair the lacerated vaginal wall during the emergency ***,the patient recovered,and no recurrence was seen in the half year of *** We review the surgical approach for such patients,analyze high-risk factors for the disease and suggest corresponding preventive measures.

关键词： Acute enterocele Complication Pelvic organ prolapse Prevention Repair Case report

Cultural Exchanges Go Beyond National Boundaries Shanghai Arts Group Tours Western Africa

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China & The World Cultural Exchange 2012年第12期78卷 30-31页

作者： wei jiao

From September 11 to 29, 2012, Shanghai Cultural and Arts Delegation made a tour to Western Africa, a follow-up action of the China-Africa Cultural Ministers’ Forum. During the tour, the 27-artists delegation presented six performances in Togo, Cape Verde and Senegal, showcasing China’s unique performing arts to Western African audiences. Celebrating the 40th Anniversary of China-Togo Diplomatic

关键词：文化艺术西非旅游国界交流塞内加尔表演艺术代表团

Medical service fees-a key step forward

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Chinese Medical Journal 2019年第15期132卷 1890-1890页

作者： wei-Ping jiao Department of Medical Insurance Management Xuanwu Hospital Capital Medical University Beijing 100053 China

First,the MSF and a set of strategies have facilitated an orderly flow of patients and have remodeled the patient distribution configuration among different levels of *** to increase financial and personnel support to community health service centers (CHSCs) have been implemented for many ***,the “inverted pyramid” distribution of patients has not been *** the implementation of the MSF,the out-of-pocket cost for attending physicians was increased at tertiary hospitals and reduced or even free at primary hospitals and *** contrast,the costs for deputy chief physicians (DCPs),chief physicians (CPs),or honor specialist physicians (HSPs) stayed the same at all levels of hospitals.

关键词： Medical service fees health service centers

Can Cadmium Uptake by Chinese Cabbage be Reduced After Growing Cd-Accumulating Rapeseed?

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Pedosphere 2010年第1期20卷 90-95页

作者： SU De-Chun , jiao wei-Ping, ZHOU Man and CHEN Xia College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193 (China) College of Resources and Environmental Sciences China Agricultural University Beijing 100193 (China)

Rapeseed （Brassica campestris L.）, a Cd-accumulating crop, is effective in reducing plant-available Cd from soil. A pot experiment was conducted to test the hypothesis that the Cd uptake by Chinese cabbage （Brassica pekinensis Rupr.） grown in rotation with rapeseed would be reduced due to the efficient removal of bioavailable Cd from soil solution by the rapeseed crops. The Cd removal by shoot for the rapeseed cultivar Zhucang Huazi ranged from 0.24% to 0.99% in natural Cd-contaminated soil （soil A） and from 0.63% to 1.23% in artificial Cd-contaminated soil （soil B） during the growth period of 4 7 weeks; whereas that for the rapeseed cultivar Chuanyou Ⅱ-93 ranged from 0.25% to 0.66% and from 0.38% to 1.02%, respectively. Chinese cabbage grown in the pots with soil A for five weeks after harvesting rapeseed exhibited a significantly lower Cd concentration compared to that grown in the control pots （uncropped with rapeseed）. However, the rotation of rapeseed did not lower the Cd concentration of Chinese cabbage on soil B. Although an increase of Cd uptake was observed in the rapeseed cultivars with growth time, and the decrease of soil NH4OAc-extractable Cd was found only in the natural rather than artificial Cd-contaminated soil.

关键词： Cd-contaminated soil crop rotation heavy metal phytoremediation plant-available Cd

布地格福吸入气雾剂与氟替美维吸入粉雾剂治疗中重度慢性阻塞性肺疾病稳定期有效性与安全性比较

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中国药业 2024年第11期33卷 84-87页

作者：任婷婷焦伟丁成红王梦珂柯兵徐丙发安徽省庐江县人民医院安徽合肥231500 安徽医科大学第三附属医院安徽合肥230032

目的比较布地格福吸入气雾剂与氟替美维吸入粉雾剂治疗中重度慢性阻塞性肺疾病(COPD)稳定期患者的有效性与安全性。方法选取安徽省庐江县人民医院2020年3月至2023年2月收治的中重度COPD稳定期患者120例,按随机数字表法分为对照组和研... 详细信息

目的比较布地格福吸入气雾剂与氟替美维吸入粉雾剂治疗中重度慢性阻塞性肺疾病(COPD)稳定期患者的有效性与安全性。方法选取安徽省庐江县人民医院2020年3月至2023年2月收治的中重度COPD稳定期患者120例,按随机数字表法分为对照组和研究组,各60例。对照组患者予氟替美维吸入粉雾剂治疗,研究组患者予布地格福吸入气雾剂治疗,两组患者均治疗3个月。结果研究组和对照组患者的总有效率、不良反应发生率均相当(86.67%比88.33%,11.67%比13.33%,P>0.05)。治疗后,两组患者的肺功能指标第1秒用力呼气容积、用力肺活量、用力肺活量占预计值百分比、第1秒用力呼气容积与用力肺活量的比值均显著升高(P0.05);两组患者的动脉血气分析指标动脉血氧分压、血氧饱和度均显著升高(P0.05);两组患者的疾病相关指标圣乔治呼吸问卷(SGRQ)评分、COPD评估测试量表(CAT)评分均显著降低(P0.05);两组患者的炎性因子C反应蛋白、白细胞介素6、降钙素原水平均显著降低(P0.05)。结论布地格福吸入气雾剂与氟替美维吸入粉雾剂治疗中重度COPD稳定期的临床疗效均良好且相当,可有效改善患者的肺功能和动脉血气指标,降低炎性因子水平,且安全性良好,临床应用时应根据患者的疾病特点及使用需求选用。

关键词：中重度慢性阻塞性肺疾病稳定期布地格福吸入气雾剂氟替美维吸入粉雾剂有效性安全性

特厚煤层坚硬顶板井下定向深孔水力压裂卸压防冲技术

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煤炭技术 2024年第9期43卷 213-216页

作者：焦伟马文涛尚楠司雷马江鹏汪永乐陕西彬长文家坡矿业有限公司陕西咸阳712000 中煤科工开采研究院有限公司北京100013

为进一步解决坚硬难垮顶板导致的冲击风险升高问题,以文家坡矿为例,提出了一种井下定向深孔水力压裂卸压防冲技术,并在4106工作面开展了试验研究,结果表明:提前对厚硬顶板进行卸压处理,岩层强度和完整性大大降低,有利于根本上消除冲击... 详细信息

为进一步解决坚硬难垮顶板导致的冲击风险升高问题,以文家坡矿为例,提出了一种井下定向深孔水力压裂卸压防冲技术,并在4106工作面开展了试验研究,结果表明:提前对厚硬顶板进行卸压处理,岩层强度和完整性大大降低,有利于根本上消除冲击地压对安全生产的严重威胁。压裂期间泵压-时间曲线呈现锯齿状,定向深孔水力压裂能够使得裂缝起裂与扩展,实现顶板有效致裂。在水力压裂作用下,工作面微震活动平稳变化,高级别能量微震事件频次占比显著降低,未超过冲击危险预警临界指标,工作面顶板活动性及微震能量释放保持在较稳定状态,围岩积聚能量能够有序释放,冲击风险显著降低。

关键词：特厚煤层坚硬顶板深孔压裂防治冲击地压