检索结果-南通市图书馆

SNAIL1 is essential for female gametogenesis in Arabidopsis thaliana

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2017年第9期59卷 629-641页

作者： Lihong Hao Xiaolin Wei Jiulei Zhu jiao shi Jingjing Liu Hongya Gu Tomohiko Tsuge Li-Jia Qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesSchool of Life SciencesPeking UniversityBeijing 100871China National Plant Gene Research Center(Beijing) Bejing 100101China Institute for Chemical Research Kyoto UniversityGokasho UjiKyoto 611-0011Japan

Two yeast Brix family members Ssfl and Ssf2, involved in large ribosomal subunit synthesis, are essential for yeast cell viability and mating efficiency. Their putative homologs exist in the Arabidopsis genome; however, their role in plant development is unknown. Here, we show that Arabidopsis thaliana SNAIL1 （AtSNAIL1）, a protein sharing high sequence identity with yeast Ssfl and Ssf2, is critical to mitosis progression of female gametophyte development. The snail1 homozygous mutant was nonviable and its heterozygous mutant was semi-sterile with shorter siliques. The mutation in SNAIL1 led to absence of female transmission and reduced male transmission. Further phenotypic analysis showed that the synchronic development of female gametophyte in the snail1 heterozygous mutant was greatly impaired and the snaih pollen tube growth, in vivo, was also compromised. Furthermore, SNAIL1 was a nucleolar- localized protein with a putative role in protein synthesis. Our data suggest that SNAIL1 may function in ribosome biogenesis like Ssf1 and Ssf2 and plays an important role during megagametogenesis in Arabidopsis.

关键词： SNAIL1 is essential for female gametogenesis in Arabidopsis thaliana Figure

Single-atom Zn confined in hierarchical hollow microstructure as an acid/base-resistant microwave absorption materials

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第8期17卷 6785-6794页

作者： Yue Lou jiao Li Xiaokun Li Zhenyu Zhu Zhan shi Biao Xu School of Chemistry and Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012China

Developing acid/base-resistant and low-price microwave-absorbing materials with lighter weight is highly desired for practical applications in extreme ***,we demonstrate the successful synthesis of the N-doped porous carbon(NC)material with hierarchical pore structure by the spray pyrolysis *** large specific surface area(SBET=707.53 m^(2)·g^(−1))of materials enables multiple scattering of incident electromagnetic waves,and N doping greatly enhances the electrical conductivity of the ***,single-atom Zn can adjust the local electronic structure of adjacent sites such as carbon and nitrogen atoms,induce the center of polarization,and thus change the dielectric and electronic properties of the host *** porous carbon coating of single-atom Zn avoids the deterioration of electromagnetic parameters caused by the accumulation of magnetic particles under high-temperature *** the same time,they can also be used in various complex environments,such as acidic and basic ***,NC-1000,with high surface area,low density,and good chemical stability,obtained a minimum reflection loss(RLmin)of−50.5 dB and an effective absorption bandwidth(EAB)exceeding 5.1 GHz at the thickness of 1.9 *** soaking in the strong acid and base solution,the electromagnetic wave absorption performance of the material decreased by<15%.Widely available raw materials and a simple preparation scheme are expected to expedite industrial mass production for this novel type of materials.

关键词： hierarchical hollow microstructure microwave absorption metal-organic framework(MOF) impedance matching singleatom Zn acid/base-resistant

Antitumor effects of different Ganoderma lucidum spore powder in cell-and zebrafish-based bioassays

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2021年第2期19卷 177-184页

作者： Yue-jiao shi Hua-xian Zheng Zhu-ping Hong Han-bo Wang Ying Wang Ming-yan Li Zhen-hao Li Zhejiang Shouxiangu Pharmaceutical Co. LtdWuyi 321200Zhejiang ProvinceChina Zhejiang Shouxiangu Botanical Drug Institute Co. LtdHangzhou 310012Zhejiang ProvinceChina Zhejiang Engineering Research Center of Rare Medicinal Plants Wuyi 321200Zhejiang ProvinceChina Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310012Zhejiang ProvinceChina

Objective:Ganoderma lucidum spore(GLS)is gaining recognition as a medicinal part of *** and has been reported to possess various pharmacological properties,such as antitumor *** this work,wall-broken GLS powder(BGLSP)and wall-removed GLS powder(RGLSP),two kinds of GLS powder with different manufacturing techniques,were compared in terms of contents of active constituents and in vivo and in vitro antitumor ***:The ultraviolet and visible spectrophotometry method was used to determine the contents of polysaccharides and total triterpenoids in BGLSP and *** individual triterpenoids were further quantified using ultra-high-performance liquid chromatography and quantitative analysis of multicomponents by single *** antitumor effects of BGLSP and RGLSP were evaluated using in vitro cell viability assay against human gastric carcinoma SGC-7901,lung carcinoma A549 and lymphoma Ramos and further validated by in vivo zebrafish xenograft models with transplanted SGC-7901,A549 and Ramos,Results:The results showed that the contents of polysaccharides,total triterpenoids and individual triterpenoids of RGLSP were significantly higher than those of *** both BGLSP and RGLSP inhibited the three tumor cell lines in vitro in a dose-dependent manner,the inhibitory effects of RGLSP were much better than those of *** the in vivo zebra fish assay,RGLSP exhibited more potent inhibitory activities against tumors transpla nted into the zebra fish compared with BGLSP,and the inhibition rates of RGLSP reached approximately 78%,31%and 83%on SGC-7901,A549 and Ramos,***:The results indicated that the antitumor effects of GLS were positively correlated with the contents of the polysaccha rides and triterpenoids and demonstrated that the wall-removing manufacturing technique could significantly improve the levels of active constituents,and thereby enhance the antitumor activity.

关键词： Ganoderma lucidum Tumor Cancer cell Zebrafish Wall-broken Ganoderma lucidum spore powder Wall-removed Ganoderma lucidum spore powder

Role of tumor necrosis factor-alpha in zebrafish retinal neurogenesis and myelination

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2016年第6期9卷 831-837页

作者： Xu-Dan Lei Yan Sun shi-jiao Cai Yang-Wu Fang Jian-Lin Cui Yu-Hao Li Key Laboratory of Tumor Microenvironment and Neurovascular Regulation Nankai University School of Medicine

AIM： To investigate the role of tumor necrosis factoralpha （TNF-α） in zebrafish retinal development and myelination. METHODS： Morpholino oligonucleotides （MO）, which are complementary to the translation start site of the wild-type embryonic zebrafish TNF-α mRNA sequence, were synthesized and injected into one to four-cell embryos. The translation blocking specificity was verified by Western blotting using an anti-TNF-α antibody, whole-mount in sltuhybridization using a hepatocytespecific mRNA probe ceruloplasmin （cp）, and coinjection of TNF-α MO and TNF-α mRNA. An atonel homolog 7 （atoh7） mRNA probe was used to detect neurogenesis onset. The retinal neurodifferentiation was analyzed by immunohistochemistry using antibodies Zn12, Zprl, and Zpr3 to label ganglion cells, cones, and rods, respectively. Myelin basic protein （mbp）was used as a marker to track and observe the myelination using whole-mount in situ hybridization. RESULTS： Targeted knockdown of TNF-α resulted in specific suppression of TNF-α expression and a severely underdeveloped liver. The co-injection of TNF-α MO and mRNA rescued the liver development. Retinal neurogenesis in TNF-cc morphants was initiated on time. The retina was fully laminated, while ganglion cells, cones, and rods were well differentiated at 72 hours post-fertilization （hpf）. mbp was expressed in Schwann cells in the lateral line nerves and cranial nerves from 3 days post -fertilization （dpf） as well as in oligodendrocytes linearly along the hindbrain bundles and the spinal cord from 4 dpf, which closely resembled its endogenous profile. CONCLUSION： TNF-α is not an essential regulator for retinal neurogenesis and optic myelination.

关键词： tumor necrosis factor-alpha retina neurogenesis myelination zebrafish

Adaptively Enhancing Facial Expression Crucial Regions via a Local Non-local Joint Network

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Machine Intelligence Research 2024年第2期21卷 331-348页

作者： Guanghui shi Shasha Mao Shuiping Gou Dandan Yan Licheng jiao Lin Xiong School of Artificial Intelligence Xidian UniversityXi'an 710000China Applied Research Laboratory Sense TimeXi'an 710000China

Facial expression recognition(FER)is still challenging due to the small interclass discrepancy in facial expression *** view of the significance of facial crucial regions for FER,many existing studies utilize the prior information from some annotated crucial points to improve the performance of ***,it is complicated and time-consuming to manually annotate facial crucial points,especially for vast wild expression *** on this,a local non-local joint network is proposed to adaptively enhance the facial crucial regions in feature learning of FER in this *** the proposed method,two parts are constructed based on facial local and non-local information,where an ensemble of multiple local networks is proposed to extract local features corresponding to multiple facial local regions and a non-local attention network is addressed to explore the significance of each local *** particular,the attention weights obtained by the non-local network are fed into the local part to achieve interactive feedback between the facial global and local ***,the non-local weights corresponding to local regions are gradually updated and higher weights are given to more crucial ***,U-Net is employed to extract the integrated features of deep semantic information and low hierarchical detail information of expression ***,experimental results illustrate that the proposed method achieves more competitive performance than several state-of-the-art methods on five benchmark datasets.

关键词： Facial expression recognition deep neural network multiple network ensemble attention network facial crucial regions

Moxibustion modulates working memory in patients with amnestic mild cognitive impairment:a functional magnetic resonance imaging study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中医杂志:英文版 2023年第4期43卷 801-808页

作者： LI Xingjie LIU Qiqi XIA Rui LIU Jun WANG Dan shi jiao KUANG Yuxing DAI Yalan HUANG Haoyu TANG Wei CHEN Shangjie College of Acupuncture and Massage Anhui University of Chinese MedicineHefei 230031China Department of Rehabilitation The People's Hospital of Baoan ShenzhenSouthern Medical Universityand the Second Affiliated Hospital of Shenzhen UniversityShenzhen 518101China

OBJECTIVE:To investigate the correlation between changes in brain activity associated with working memory and assessment scales of memory scores in amnestic mild cognitive impairment(aMCI)before and after moxibustion ***:aMCI patients were randomized into the moxibustion treatment(MT)group and the placebo moxibustion(PM)*** group received either moxibustion therapy or a placebo moxibustion for eight *** performance and functional brain responses to a working memory task were assessed at baseline and at the end of *** function was evaluated individually by the Rivermead behavioral memory test(RBMT),and working memory was assessed by the N-back ***:Compared with the PM group,RBMT score changes were significant(P<0.05).In the MT group,the accuracy of the N-back texts increased compared with those before the *** moxibustion intervention,the right insula,postcentral gyrus,precentral gyrus,superior temporal gyrus,thalamus,lingual gyrus,calcarine sulcus,posterior cingulate gyrus,middle frontal gyrus and anterior frontal gyrus were significantly activated(P=0.01,Cluster-level Family-Wise Error=0.05).Pearson correlation analysis showed that the insula,lingual gyrus and posterior cingulate gyrus were associated with changes in N-back *** correlation analysis showed that the postcentral gyrus,superior temporal gyrus,thalamus,lingual gyrus,and posterior cingulate gyrus were correlated with RBMT score ***:Moxibustion treatment improved memory in aMCI patients and was associated with the activation of the brain region of the insula,lingual gyrus,posterior cingulate gyrus,postcentral gyrus,superior temporal gyrus,and thalamus,which may be an important mechanism by which moxibustion improves the memory function.

关键词： memory short-term cognitive dysfunction magnetic resonance imaging moxibustion task performance and analysis

基于MQTT协议的轻量化文本信息分发技术研究

在线全文

学校读者我要写书评

暂无评论

计算机技术与发展 2024年第2期34卷 90-97页

作者：熊风光陈霖韩慧妍张元庞敏焦世超中北大学计算机科学与技术山西太原030051 机器视觉与虚拟现实山西省重点实验室山西太原030051 山西省视觉信息处理及智能机器人工程研究中心山西太原030051

随着卫星通信网络的发展,基于卫星通信网络实现手机、平板电脑等移动通信设备之间的通信成为研究的热点。由于其采用无线电波进行信号传输,且需要经过多个中继站进行信号的转发和处理,导致存在网络延时大、丢包率高以及信道狭窄等问题... 详细信息

随着卫星通信网络的发展,基于卫星通信网络实现手机、平板电脑等移动通信设备之间的通信成为研究的热点。由于其采用无线电波进行信号传输,且需要经过多个中继站进行信号的转发和处理,导致存在网络延时大、丢包率高以及信道狭窄等问题。针对文本信息在卫星通信网络下的分发过程存在效率低下、可靠性不高的问题,设计一种基于MQTT(Message Queuing Telemetry Transport)协议的轻量化文本信息分发技术。该技术使用MQTT协议作为消息传输协议,在文本信息分发前对MQTT协议进行主题设计、发布订阅机制设计、设备连接设计以及设备心跳设计,确保设备之间的连通性;在文本信息的分发过程中,设计数据校验加密算法、文本信息轻量化处理方法和离线消息存储机制,保证文本信息分发的安全性、可靠性。实验结果表明:相较于传统的基于JSON数据的文本信息分发技术,该技术在提高文本信息分发效率的同时,可确保信息分发的安全性、完整性和稳定性。

关键词： MQTT协议轻量化文本信息分发数据加密离线消息存储

Catalytic asymmetric(3+3)cycloaddition between different 2-indolylmethanols

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2024年第8期67卷 2629-2636页

作者： Tianzhen Li Sijia Liu Shufang Wu Qi Cheng Qun Chen Yinchun jiao Yuchen Zhang Feng shi School of Petrochemical Engineering School of Materials ScienceEngineeringChangzhou UniversityChangzhou 213164China Research Center of Chiral Functional Heterocycles School of Chemistry and Materials ScienceJiangsu Normal UniversityXuzhou 221116China School of Chemistry and Chemical Engineering Hunan University of Science and TechnologyXiangtan 411201China School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007China

The catalytic asymmetric(3+3)cycloaddition between different 2-indolylmethanols has been established,in which a series of chiral indole-fused six-membered heterocycles were constructed in high yields with excellent *** work not only has established the first catalytic asymmetric cycloaddition between different 2-indolylmethanols but also provided a powerful strategy for constructing enantioenriched indole-fused six-membered ***,biological evaluation discovered some products with promising antitumor ***,theoretical calculations performed on the reaction pathway and activation mode provide an in-depth understanding of this catalytic asymmetric(3+3)cycloaddition among different 2-indolylmethanols,which will advance the understanding of the chemistry of indolylmethanols.

关键词： asymmetric organocatalysis chiral phosphoric acid cycloaddition enantioselectivity 2-indolylmethanol

Single-wall carbon nanotube fiber non-woven fabrics with a high electrothermal heating response

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第6期17卷 5621-5628页

作者： Xinyu jiao Lele Xu Xinyang Sun Chao shi Peng-Xiang Hou Chang Liu Hui-Ming Cheng Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaHefei 230026China Faculty of Materials Science and Engineering/Institute of Technology for Carbon Neutrality Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Carbon nanotube(CNT)fibers have great promise for constructing multifunctional fabrics with high electrical conductivity,good electro-heating ability,excellent flexibility,and a low ***,the inter-fiber contacts in the fabric greatly reduce these advantages and limit their ***,a simple pressure-fusing method to fabricate single-wall CNT(SWCNT)fiber non-woven fabrics(NWFs)that are composed of interconnected SWCNT fibers with fused joints is reported,which have good flexibility,a low density of 0.46 g/cm^(3),a high electrical conductivity of 3.7×10^(5)S/m,and a record high specific electrical conductivity of 803(S·m^(2))/*** also showed excellent electrical heating ability,so that a temperature of~160℃was rapidly reached at a low voltage of 2 *** with their low density,the SWCNT fiber NWFs are promising for use as a heating unit for low temperature battery protection and de-icing applications.

关键词： carbon nanotube fiber nonwoven fabrics fusing electrothermal heating