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Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4

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Journal of Animal Science and Biotechnology 2017年第1期8卷 43-50页

作者： Yan Li Di Lian Shoulong Deng Xiaosheng Zhang Jinlong Zhang Wenting Li Hai Bai Zhixian Wang Hongping Wu Juncai Fu Hongbing Han jianzhong feng Guoshi Liu Ling Lian Zhengxing Lian Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture Beijing Key Laboratory for Animal Genetic Improvement College of Animal Science and Technology China Agricultural University Beijing 100193 China Department of Public Health Benedictine University Lisle IL 60532 USA State Key Laboratory of Reproductive Biology institute of Zoology Chinese Academy of Sciences Beijing 100101 China Tianjin Institute of Animal Sciences Tianjin 300381 China

Background： Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons（breeding and non-breeding season） on superovulation and the imported exogenous gene on ***： In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic *** results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons（P 〉 0.05）. The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group（P 〈 0.05）. The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any ***： Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep.

关键词： Growth Microinjection Pronuclear embryos Sheep Superovulation

Protoplast Culture and Its Application in Fruit Breeding

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Agricultural Biotechnology 2019年第3期8卷 147-149,152页

作者： Xiangmin SUO Jie HAO Xinmin YAN Xiange WANG jianzhong feng Xueying LI Shijiazhuang Pomology Institute Hebei Academy of Agriculture and Forestry Sciences

The progress in protoplast technology and its application in fruit breeding were summarized,and the existing problems and application prospect were also discussed.

关键词： Protoplast culture Protoplast fusion Cybrids Fruit breeding

Application of Biotechnology on Apple Breeding

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Agricultural Biotechnology 2019年第2期8卷 55-58页

作者： Xueying LI Jie HAO Xinmin YAN Xian'ge WANG jianzhong feng Xiangmin SUO Shijiazhuang Pomology Institute Hebei Academy of Agriculture and Forestry Sciences

Modern biotechnology is a kind of potential technology. The advantages of this method have aroused much attention. Recently, biotechnology has evolved very fast in apple tree, and has got much progress in production and breeding of seedlings. This paper summarized the advances in tissue culture, molecular marker and gene engineering. The emphases and direction which need further investigation were also discussed.

关键词： Apple Biotechnology Tissue culture Molecular marker Gene engineering

Metallogenic characteristics of epithermal gold deposits in China

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Chinese Science Bulletin 1999年第S2期44卷 136-138页

作者： feng jianzhong~1 and WANG Jingbin~2 1. Department of Geology, Peking University, Beijing 100871, China 2. Beijing Institute of Geology For Mineral Resources, Beijing 100012, China Department of Geology Peking University Beijing 100871 China Beijing Institute of Geology for Mineral Resources Beijing 100012 China

VOLCANIC-SUBVOLCANIC rocks are extensively distributed in China, in which a series of epithermal golddeposits related to volcanism have been discovered. 1 Metallogenic tectonic settings of epithermal gold deposits The epithermal gold deposits are mainly formed under the following tectonic settings: (i) ancient island arc collision, such as Xingxingxia island arc, Armantai island arc, Beishan island arc and Jueluotage island arc, etc. in periphery of Gunggar, Altai, Baishan, etc. in ancient Europe domain; (ii) extension area of continental margin of back arc, such as Fujian, Zhejiang, Jiangsu and Henlongjiang, *** circum-Pacific metallogenic domain; (iii) Mesozoic-Cenozoic island arc, such as the Tethys-Himalayametallogenic domain and Taiwan; (iV) intracontinental faulting depression or intraplate deep-seatedfaults, such as northern Hebei, Liaoning, Inner Mongolia at the north margin of the North China platformand periphery of Taicheng-Loujiang fault.

关键词： metallogenic characteristics gold deposits China.

Analysis on Factors Related with Slender Spindle Shaped Apple Trees

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Agricultural Biotechnology 2019年第1期8卷 28-30,33页

作者： Jie HAO Xiange WANG Xueying LI Xiangmin SUO Liang WEI Xinmin YAN jianzhong feng Shijiazhuang Pomology Institute Hebei Academy of Agriculture and Forestry Sciences Department of Natural Resources of Hebei province

[Objectives] This study was to determine the correlations of stem taperingness with small central shaft total roughness,average roughness,total length,and average length by slender spindle pruning mode on different apple cultivars. [Methods]A total of 2 tree structures were selected,namely,the slender spindleshaped Zhongqiuwang from Shen County of Hebei Province and slender spindle-shaped short shoot Fuji from Xingtang County of Hebei Province,to investigate the growth indicators of the small central shafts. SPSS analysis was used to compare the correlations between the taperingness with the growth factors of the small central shafts. [Results]The stem taperingness on slender spindle-shaped Zhongqiuwang was positively correlated with the various growth factors of the small central shaft,and the difference in the positive correlation with average roughness reached the extremely significant level. The stem taperingness of slender spindle-shaped short shoot Fuji was negatively correlated with the total roughness of small central shaft,but was positively correlated with the average roughness,total length and average length. [Conclusions] The results provided scientific and theoretical basis for guiding the dense planting of dwarfing apple tree pruning technology.

关键词： Apple Slender spindle pruning Related factors analysis

Technical Specifications for Integrated Prevention and Control of Yellow Aphids

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Asian Agricultural Research 2021年第3期13卷 52-56页

作者： Jie HAO Jinxin WANG Jingmiao HUANG Xueying LI Xinmin YAN jianzhong feng Xiangmin SUO Shijiazhuang Pomology Institute Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050061China

[Objectives]The purpose was to standardize the scientific prevention and control of yellow aphids and effectively control their occurrence and spread.[Methods]On the basis of consulting domestic and foreign data and standards extensively,a series of related trials were carried out.[Results]Technical specifications for integrated prevention and control of yellow aphids were formulated.[Conclusions]It is of great significance to the healthy and sustainable development of the fruit trees’industry,the implementation of standardized management,the reduction of pesticide uses,and the reduction of damage caused by yellow aphids to fruit trees,and also provides urgent-needed technical support and guarantee for the prevention and control of yellow aphids.

关键词： Yellow aphids Integrated prevention and control Technical specification

农村振兴背景下信息服务投入效应对创业绩效影响机制研究

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农业图书情报学报 2022年第6期34卷 50-60页

作者：王剑高峰冯建中中国农业科学院农业信息研究所北京100081 农业部农业信息服务技术重点实验室北京100081 天津社会科学院经济社会预测研究所天津300191

[目的 /意义]在农村创业热潮不断推进的背景下,探索农业信息服务赋能农民创业绩效的影响关系,对加速科技成果转化、驱动农村创业具有重要意义。[方法 /过程]在全信息转化的理论框架下,基于农村返乡入乡创业创新试点监测工作的相关调查数... 详细信息

[目的 /意义]在农村创业热潮不断推进的背景下,探索农业信息服务赋能农民创业绩效的影响关系,对加速科技成果转化、驱动农村创业具有重要意义。[方法 /过程]在全信息转化的理论框架下,基于农村返乡入乡创业创新试点监测工作的相关调查数据,以新型高素质农民为研究对象,搭建以信息服务供给水平为主变量的门限模型,从服务转化能力的角度,深入探索信息服务对新型高素质农民创业绩效的影响机制,以剖析信息服务与创业绩效的内在作用机理。[结果 /结论]研究结果显示,农业信息服务对新型高素质农民创业绩效的影响并不是简单的线性促进关系,而是呈现出一种“单一门限”效应的非线性关系,当农业信息服务供给水平超过一定的门限阀值时,信息服务对创业绩效的正向促进作用降低了43.6%。这表明,IT生产率悖论也存在于农村创业发展过程中,因此,应当根据实际情况制定农业信息服务供给策略,避免过度投入,同时还应结合提升农民教育与信息化素质、加快农村创业基础设施建设等措施,实现农村创业质量的有效提升。

关键词：创业绩效信息服务供给水平门限模型新型高素质农民信息扶贫农民创业

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Effects of Different Dwarfing Rootstocks on Growth,Yield and Fruit Quality of‘Tianhong 2’Apple Trees

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Asian Agricultural Research 2023年第5期15卷 36-40页

作者： Jinxin WANG Jingmiao HUANG Jianming LI Xiangmin SUO Xueying LI jianzhong feng Jie HAO Shijiazhuang Pomology Institute Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050061China

[Objectives]The paper was to provide a reference for screening dwarfing rootstock suitable for main spur-type Fuji cultivars in central and southern Hebei Province.[Methods]With spur-type Fuji‘Tianhong 2’as the material,the vegetative growth,yield and fruit quality of 8 different rootstock-scion combinations were compared.[Results]‘Tianhong 2’/SH6 as self-rooted rootstock had large average single fruit weight(256.33 g),large number of fruits per plant(188.68),the highest yield[(3250.08±23.42)kg/667 m ^(2)]and the highest colored area(93.5%),and the soluble solid content reached the requirement of high quality fruit(15.78%).[Conclusions]In central and southern Hebei Province,‘Tianhong 2’grafted on SH6 self-rooted rootstock has moderate growth,high yield and good fruit quality,so it can be considered as the preferred rootstock-scion combination in the local area.

关键词： Apple Dwarfing rootstock Growth characteristics Yield Quality

Analysis of Short-Shoot Fuji Apple Tree Structure and Correlative Factors

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Asian Agricultural Research 2017年第6期9卷 86-88页

作者： Jie HAO Xiangmin SUO Xueying LI Liang WEI Xinmin YAN Xian’ge WANG jianzhong feng Shijiazhuang Pomology Institute Hebei Academy of Agriculture and Forestry Sciences Hebei Provincial Department of Land and Resources

In order to clarify the correlation between short-shoot Fuji apple tree structure and different factors under different trimming and pruning modes,we investigate the trunk taperingness of free-spindle short-shoot Fuji and slender-spindle short-shoot Fuji,respectively,as well as the total thickness,average thickness,total length and average length of small main branches in the standard demonstration apple garden in Xingtang County of Hebei Province. By SPSS analysis,we study the correlation between trunk taperingness of trees with different shapes and the growth indices of their small main branches. The results show that the trunk taperingness of free-spindle short-shoot Fuji apple is negatively correlated with the total thickness,average thickness,total length and average length of small main branches,but the correlation is not significant; the trunk taperingness of slender-spindle short-shoot Fuji apple is negatively correlated with the total thickness but positively correlated with other factors,and the correlation with average length reaches a significant level. The results of this study can provide a scientific basis for guiding the high-density dwarf rootstock short-shoot Fuji apple tree trimming technology.

关键词： Short-shoot Fuji Tree structure Analysis of correlative factors