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M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

Science China(Life Sciences) 2024年第3期67卷 579-595页

作者： Yuzhen Hu Li Jiang Guangwen Wang Yangming Song Zhibo Shan Xuyuan Wang Guohua Deng jianzhong Shi Guobin Tian Xianying Zeng Liling Liu Hualan Chen Chengjun Li State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

关键词： influenza A virus M6PR HA membrane fusion late endosome

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从文化视角看机构知识库的建设与创新

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中国图书馆学报 2024年第1期50卷 58-65页

作者：吴建中澳门大学图书馆

学术成果传播是现代大学的核心使命之一，起步于2000年前后的机构知识库在大学履行该使命中扮演了不可或缺的角色。机构知识库初期建设的重心在于对正式发表的学术成果的汇集和开放获取，随着学术研究活动越趋数字化和多样化，机构知识... 详细信息

学术成果传播是现代大学的核心使命之一，起步于2000年前后的机构知识库在大学履行该使命中扮演了不可或缺的角色。机构知识库初期建设的重心在于对正式发表的学术成果的汇集和开放获取，随着学术研究活动越趋数字化和多样化，机构知识库的功能及服务方式进一步深化。近二十年图书馆转型的实践表明，机构知识库建设不只是技术或专业层面的问题，更与价值观念、服务方式以及与用户之间的合作等密切相关。机构知识库在不断深化和创新的同时，也给机构管理及文化带来一系列新的挑战，需要从文化的视角思考和解决发展中的问题。本文从大学及研究型图书馆角度出发，从三个方面探讨机构知识库发展中面临的挑战与机遇：(1)走出图书馆，在与用户沟通中发现新需求；(2)拓宽新渠道，实现馆内资源的纵向整合；(3)拥抱新时代，以全球共享为目标履行新使命。对机构知识库下一轮发展提出三点建议：注重全范围参与，加强集体合作与共享，深化高质量服务。参考文献27。

关键词：机构知识库图书馆转型大学图书馆全球共享知识库学术成果传播

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A novel preimplantation genetic testing strategy for a subtelomeric genetic disorder:A case study

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Genes & Diseases 2024年第4期11卷 13-15页

作者： Songchang Chen Li Wanng Luting Chen Weihui Shi Junyu Zhang Yuting Hu Yinyu Wang Li Jin jianzhong Sheng Feng Zhang Yanting Wu Hefeng Huang Chenming Xu Obstetrics and Gynecology Hospital Institute of Reproduction and DevelopmentFudan UniversityShanghai 200090China The International Peace Maternity and Child Health Hospital School of MedicineShanghai Jiao Tong UniversityShanghai 200030China Research Units of Embryo Original Diseases Chinese Academy of Medical SciencesShanghai 200030China Key Laboratory of Reproductive Genetics(Ministry of Education) Department of Reproductive EndocrinologyWomen's HospitalZhejiang University School of MedicineHangzhouZhejiang 310058China Shanghai Key Laboratory of Embryo Original Diseases Shanghai 200030China Shanghai First Maternity and Infant Hospital Tongji University School of MedicineShanghai 200030China

Preimplantation genetic testing(PGT)is a precise and effective technique for detecting inherited pathogenic mutations to prevent birth ***,there are few reports on PGT for pseudo-autosomal genetic diseases.

关键词： implantation testing precise

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Enhancing m^(6)A modification in the motor cortex facilitates corticospinal tract remodeling after spinal cord injury

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Neural Regeneration Research 2025年第6期20卷 1749-1763页

作者： Tian Qin Yuxin Jin Yiming Qin Feifei Yuan Hongbin Lu jianzhong Hu Yong Cao Chengjun Li Department of Spine Surgery and Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan ProvinceChangshaHunan ProvinceChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Sports Medicine Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

Spinal cord injury typically causes corticospinal tract disruption. Although the disrupted corticospinal tract can self-regenerate to a certain degree, the underlying mechanism of this process is still unclear. N6-methyladenosine(m^(6)A) modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes. However, whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown. We found that expression of methyltransferase 14 protein(METTL14) in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels. Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury. Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction, we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner, thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration. Finally, we administered syringin, a stabilizer of METTL14, using molecular docking. Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14. Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.

关键词： corticospinal tract remodeling epigenetic regulations locomotor cortex m^(6)A modification methyltransferase 14 protein(METTL14) mitogen-activated protein kinase neural regeneration spinal cord injury syringin TRIB2

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生物钟蛋白Rev-erbα激动剂抑制高脂饮食诱导小鼠前列腺组织炎症的实验研究

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中国实验动物学报 2022年第4期30卷 469-475页

作者：何跃徐航冯德超曹德宏艾建忠杨璐魏强遂宁市中心医院泌尿外科四川遂宁629000 四川大学华西医院泌尿外科/泌尿外科研究所成都610041

目的验证生物钟蛋白Rev-erbα激动剂是否能够抑制高脂饮食诱导的小鼠前列腺组织炎症,并探讨其机制。方法C57B/6J雄性小鼠分为4组:普通饮食组;高脂饮食组;高脂饮食+SR9009组;高脂饮食+control组。检测小鼠体重、外周血胆固醇(TC)、甘油三... 详细信息

目的验证生物钟蛋白Rev-erbα激动剂是否能够抑制高脂饮食诱导的小鼠前列腺组织炎症,并探讨其机制。方法C57B/6J雄性小鼠分为4组:普通饮食组;高脂饮食组;高脂饮食+SR9009组;高脂饮食+control组。检测小鼠体重、外周血胆固醇(TC)、甘油三酯(TG)及前列腺重量。前列腺组织HE染色并转录组测序,免疫组化评估IL-6、IL-8、IL-1β、NF-κB-P65和CCL21的表达水平。结果与普通饮食组相比,高脂饮食组小鼠的体重、前列腺指数、血清TG和TC水平均明显升高,高脂饮食+SR9009组小鼠的体重、前列腺指数、血清TG和TC水平明显低于高脂饮食+control组。HE染色,高脂饮食组小鼠前列腺组织内炎性细胞浸润较普通饮食组增多,SR9009干预后的高脂饮食小鼠前列腺组织炎性细胞浸润减少。转录组测序差异基因分析NF-κB通路中的Ccl21a、Ccr7、Tlr4、Il6、Il1b、Cxcr2的表达水平在高脂饮食组较普通饮食组升高,而其表达水平在高脂饮食+SR9009组较高脂饮食+control组降低。IL-6、IL-8、IL-1β、NF-κB-P65和CCL21a在4组小鼠前列腺中的表达强度差异趋势与测序结果的差异趋势一致。结论生物钟蛋白Rev-erbα可能通过NF-κB通路调节高脂饮食诱导的前列腺组织炎症水平。

关键词：组织炎症生物钟蛋白Rev-erbα 前列腺小鼠

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Cretaceous integrative stratigraphy,biotas,and paleogeographical evolution of the Qinghai-Tibetan Plateau and its surrounding areas

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Science China Earth Sciences 2024年第4期67卷 1229-1289页

作者： Dangpeng XI Guobiao LI Shijun JIANG Xin RAO Tian JIANG Tianyang WANG Zuohuan QIN Yasu WANG jianzhong JIA Muhammad KAMRAN Zhongye SHI Yuyang WU Panxi WANG Lixin SUN Qinggao ZENG Xiaoqiao WAN State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesBeijing 100083China College of Oceanography Hohai UniversityNanjing 213200China Key Laboratory of Palaeobiology and Petroleum Stratigraphy Nanjing Institute of Geology and PaleontologyChinese Academy of SciencesNanjing 210008China State Key Laboratory of Tibetan Plateau Earth System Environmentand Resources(TPESER)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China China National Offshore Oil Corporation Limited Beijing 100028China Shanghai Science and Technology Museum Shanghai 200120China Xizang Bureau of Geological and Mineral Exploration and Development Lhasa 850000China Tianjin Geological Survey Center of China Geological Survey Tianjin 300170China

The Cretaceous Period is a vital time interval in deciphering the evolutionary history of the Neo-Tethys Ocean and the convergence of different plates and blocks across the Qinghai-Tibetan Plateau.A detailed stratigraphic framework and paleogeographic patterns are the basis for understanding the evolution of the Neo-Tethys Ocean and the formation of the QinghaiTibetan ***,the Cretaceous stratigraphy,biota,paleogeography,and major geological events in the Qinghai-Tibetan Plateau are analyzed to establish an integrative stratigraphic framework,reconstruct the paleogeography during the Cretaceous Period,and decode the history of the major geological *** Cretaceous rocks of the Qinghai-Tibetan Plateau and its surrounding area are predominantly marine deposits,with a small amount of interbedded marine-terrestrial and terrestrial *** Indus-Yarlung Tsangpo Suture Zone was responsible for the deposition of deep marine sediments dominated by ophiolite suites and radiolarian *** the south,the Tethys Himalayas and Indus Basin received marine sediments of varying depths and lithology;to the north,the Xigaze and Ladakh forearc basins are also filled with marine *** Lhasa Block,Karakorum Block,western Tarim Basin,and West Burma block consist of shallow marine,interbedded marine-terrestrial,and terrestrial *** Qiangtang Basin and other areas are dominated by terrestrial *** Cretaceous strata of the Qinghai-Tibetan Plateau and its surrounding areas are widely distributed and diversified,with abundant foraminifera,calcareous nannofossils,radiolarians,ammonites,bivalves,and *** the basis of integrated lithostratigraphic,biostratigraphic,geochronologic,and chemostratigraphic analyses,we proposed herein a comprehensive stratigraphic framework for the Cretaceous Period of the eastern *** analyzing the Cretaceous biota of different biogeographic zones from eastern Neo-Tethys and its surrounding a

关键词： Qinghai-Tibetan Plateau Tethys Cretaceous Stratigraphy Biota Paleogeography

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二甲双胍的作用机制新进展

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中华糖尿病杂志 2024年第7期16卷 735-739页

作者：肖建中北京清华长庚医院内分泌与代谢科北京100093

二甲双胍在临床应用已超过60年,作为一线降糖治疗药物,在糖尿病管理中发挥重要作用。随着临床证据的积累,不断有研究报道二甲双胍在糖尿病预防、减少糖尿病并发症、抗炎、抗衰老及抗肿瘤方面的获益。二甲双胍作用的核心为激活腺苷酸活... 详细信息

二甲双胍在临床应用已超过60年,作为一线降糖治疗药物,在糖尿病管理中发挥重要作用。随着临床证据的积累,不断有研究报道二甲双胍在糖尿病预防、减少糖尿病并发症、抗炎、抗衰老及抗肿瘤方面的获益。二甲双胍作用的核心为激活腺苷酸活化蛋白激酶(AMPK),另外其还通过调节线粒体稳态、改善炎症、自噬及免疫调节等方式直接或间接发挥作用,减轻不同疾病对靶细胞的损伤。该文汇总分析二甲双胍最新作用机制的研究进展,为其临床获益背后的作用特点提供探讨和思考。

关键词：糖尿病二甲双胍作用机制腺苷酸活化蛋白激酶并发症汇总分析

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Efficacy and safety of bilastine *** for the treatment of chronic idiopathic urticaria:A multicenter,double-blind,double-dummy,phase III,non-inferiority,randomized clinical trial

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Chinese Medical Journal 2024年第12期137卷 1480-1482页

作者： Xue Chen Xiuping Han Bo Cheng Hui Li Lingling Liu Songmei Geng Linfeng Li Jie Li Qing Guo Guoyi Zhang Hong Fang jianzhong Zhang Department of Dermatology Peking University People’s HospitalBeijing 100044China Department of Dermatology Shengjing Hospital of China Medical UniversityShenyangLiaoning 110055China Department of Dermatology The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian 350004China Department of Dermatology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Dermatology Peking University First HospitalBeijing 100034China Department of Dermatology The Second Affiliated Hospital of Xi’an Jiaotong University(Xibei Hospital)Xi’anShaanxi 710006China Department of Dermatology Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Dermatology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Dermatology Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouGuangdong 510120China Department of Dermatology Hospital for Skin DiseasesChinese Academy of Medical SciencesNanjingJiangsu 210042China Department of Dermatology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang 310003China

To the Editor:Chronic urticaria,prevalent globally and increasing in China,predominantly affects young and middle-aged women,impacting their quality of life(QoL).[1]Standard treatment involves second-generation H1 antihistamines,with bilastine showing promise due to its selective action and minimal side ***,direct comparative studies are scarce,particularly in the Chinese *** aimed to evaluate the efcacy and safety of bilastine compared to levocetirizine in Chinese patients.

关键词： treatment double globally

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Transmission of ceftazidime-avibactam-resistant Escherichia coli among pets,veterinarians and animal hospital environment

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Biosafety and Health 2024年第3期6卷 191-198页

作者： Hegen Dai Dongyan Shao Yu Song Qi An Zhenbiao Zhang Haixia Zhang Siyu Chen Congming Wu jianzhong Shen Yanli Lyu Yang Wang Shizhen Ma Zhaofei Xia National Key Laboratory of Veterinary Public Health Safety College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Key Laboratory of Animal Antimicrobial Resistance Surveillance Ministry of Agriculture and Rural AffairsCollege of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Department of Clinical Veterinary Medicine College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Shanxi Key Laboratory for Modernization of TCVM College of Veterinary MedicineShanxi Agricultural UniversityTaiyuan 030801China Ruichen Pet Hospital Group Co. LtdXiamen 361004China

Ceftazidime-avibactam (CZA) is a recently approved combination synthetic β-lactamase inhibitor used in human clinical medicine. Cases of CZA resistance in humans have already been reported, but limited research has investigated CZA resistance in pets. This study explored the prevalence and transmission of CZA-resistant Escherichia coli (CZAREC) among pets, their owners, veterinarians, and the environment in animal hospitals. A total of 5,419 clinical samples were collected from dogs and cats, along with samples from the environment (n = 5,843), veterinarians (n = 557), and pet owners (n = 368) in animal hospitals. From these samples, 760 Escherichia coli (E. coli) isolates were obtained, out of which 60 were identified as CZAREC. These included 34 isolates from the environment (9.14 %, n = 372), three from veterinarians (8.11 %, n = 37), and 23 from animals (6.82 %, n = 337). No CZAREC isolates were found in pet owners. The predominant sequence types of CZARECs were ST156 (n = 20), ST410 (n = 19) and ST101 (n = 7). Bayesian analysis revealed six clusters comprising 47 isolates from the hospital environment, pets, and veterinaries, displaying genetic relatedness of less than 100 core genome single nucleotide polymorphisms (cgSNPs) between any two isolates in each cluster. Some CZAREC isolates with high genetic similarity persisted in the same animal hospital for four to six months. Moreover, discriminant analysis of principal components indicated that most isolates from different hosts shared a genetic source in the human/dog/cat merged cluster. Overall, evidence of CZARECs transmission was found among pets, the environment, and veterinarians in animal hospitals. The findings emphasize the importance of monitoring CZARECs in the veterinary clinical setting to ensure the health of both pets and humans.

关键词： Ceftazidime-avibactam(CZA) Escherichia coli(E.coli) Pets Animal hospital environment Veterinarians

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Key Issues for Modelling, Operation, Management and Diagnosis of Lithium Batteries: Current States and Prospects

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Energy Engineering 2024年第8期121卷 2085-2091页

作者： Bo Yang Yucun Qian jianzhong Xu Yaxing Ren Yixuan Chen Faculty of Electric Power Engineering Kunming University of Science and TechnologyKunming650500China State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources North China Electric Power UniversityBeijing102206China School of Engineering University of LincolnLincolnLN67TSUK Department of Electrical and Electronic Engineering The University of Hong KongHong Kong999077China

1 *** the continuous growth of the global population,the energy demand continues to ***,due to the dominance of fossil fuels in global energy and fossil fuels are non-renewable,it has led to the global energy crisis[1].Besides,the use of fossil fuels will generate a mass of air pollutants(e.g.,carbon dioxide,sulfur dioxide,etc.),which will cause serious environmental pollution,climate change[2],*** resolve the aforementioned issues,countries around the world have implemented a variety of measures hoping to fundamentally adjust the global energy structure and achieve sustainable ***,“Paris Agreement”reached in 2015 under the framework of“United Nations Framework Convention on Climate Change”aims to control the increase in the average temperature of the globe to within 2°C below preindustrial levels,and thereafter to peak global greenhouse gas emissions as soon as possible,continuously decreasing thereafter[3].United Kingdom plans to reduce the average exhaust emissions of“new cars”to approximately 50–70 g/km by 20230,which is roughly half of what it is now[4].In addition,China proposed a plan at“United Nations General Assembly”in 2020 to peak carbon dioxide emissions by 2030 and strive to achieve carbon neutrality by *** is a fact that the whole world is committed to changing the current energy structure,protecting the Earth’s ecology,and achieving global sustainable development[5].

关键词： Lithium batteries optimization operation modelling state estimation life prediction fault diagnosis

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