检索结果-南通市图书馆

同方期刊数据库

Phase separation of S-RNase promotes self-incompatibility in Petunia hybrida

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2024年第5期66卷 986-1006页

作者： Huayang Tian Hongkui Zhang Huaqiu huang Yu'e Zhang Yongbiao Xue The State Key Laboratory of Plant Cell and Chromosome engineering Institute of Genetics and Developmental Biologythe Chinese Academy of SciencesBeijing 100101China University of the Chinese Academy of Sciences Beijing 100049China Beiing Institute of Genomics Chinese Academy of SciencesNational Center for BioinformationBeijing 100101China

Self-incompatibility(SI)is an intraspecific reproductive barrier widely present in *** SI system with the broadest occurrence in angiosperms is based on an S-RNase linked to a cluster of multiple S-locus F-box(SLF)genes found in the Solanaceae,Plantaginaceae,Rosaceae,and *** studies reveal that non-self S-RNase is degraded by the Skip Cullin F-box(SCF)SLF-mediated ubiquitin–proteasome system in a collaborative manner in Petunia,but how self-RNase functions largely remains ***,we show that S-RNases form S-RNase condensates(SRCs)in the self-pollen tube cytoplasm through phase separation and the disruption of SRC formation breaks SI in self-incompatible Petunia *** further find that the pistil SI factors of a small asparagine-rich protein HT-B and thioredoxin h together with a reduced state of the pollen tube all promote the expansion of SRCs,which then sequester several actin-binding proteins,including the actin polymerization factor PhABRACL,the actin polymerization activity of which is reduced by S-RNase in ***,we find that S-RNase variants lacking condensation ability fail to recruit PhABRACL and are unable to induce actin foci formation required for pollen tube growth *** together,our results demonstrate that phase separation of S-RNase promotes SI response in ***,revealing a new mode of S-RNase action.

关键词： self-incompatibility S-RNase phase separation

electricity-Heat-Based Integrated Demand Response Considering Double Auction energy Market with Multi-energy Storage for Interconnected Areas

在线全文

学校读者我要写书评

暂无评论

CSee Journal of Power and energy Systems 2024年第4期10卷 1688-1700页

作者： Dan Wang Deyu huang Qing'e Hu Hongjie Jia Bo Liu Yang Lei Key Laboratory of Smart Grid of Ministry of education Tianjin UniversityTianjin 300072China Key Laboratory of Smart energy&Information Technology of Tianjin Municipality Tianjin 30072China

With development of integrated energy systems and energy markets,transactive energy has received increasing attention from society and academia,and realization of energy distribution and integrated demand response through market transactions has become a current research *** on optimized operation of a distributed energy station as a regional energy supply center is of great significance for improving flexibility and reliability of the *** on retail-side energy trading market,this study first establishes a framework of combined electric and heating energy markets and analyses a double auction market mechanism model of interconnected distributed energy *** study establishes a mechanism model of energy market participants,and establishes the electric heating combined market-clearing model to maximize global surplus considering multi-energy ***,in the case study,a typical user energy consumption scenario in winter is selected,showing market-clearing results and demand response effects on a typical *** of transmission line constraints,energy supply equipment capacity,and other factors on clearing results and global surplus are compared and analyzed,verifying the effects of the proposed method on improving global surplus,enhancing interests of market participants and realizing coordination and optimal allocation of both supply and demand resources through energy complementarity between regions.

关键词： Double auction energy market integrated energy system interconnected energy stations multi-energy storage

Betulinic acid protects against ovarian impairment by decreasing F-2 toxin-induced oxidative stress and inflammation associated with the downregulation of p38 expression in mice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第3期13卷 1292-1302页

作者： Li Kong Xinyu Gao Lijuan Zhu Xing Lin You huang Chunlin huang Wenjiang Yang Yazhi Chen Haoqiang Zhao Jing Wu Zhihang Yuan Jin’e Yi Hunan engineering Research Center of Livestock and Poultry Health Care College of Veterinary MedicineHunan Agricultural UniversityChangsha 410128China

F-2 toxin is an estrogenic mycotoxin that causes reproductive disorders in *** acid(BA)is a natural pentacyclic lupane-structure triterpenoid that has diverse pharmacological *** this study,the antioxidative and anti-inflammatory effects of BA and its underlying mechanism are explored in F-2 toxin-triggered mouse ovarian *** found that BA alleviated the F-2 toxin-induced ovarian impairment by stimulating follicle growth,reducing inflammatory cell infiltration,repairing damaged mitochondria and endoplasmic ***,BA not only reversed F-2 toxin-induced reduction of follicle stimulating hormone(FSH)and luteinizing hormone(LH)levels in the serum,but also restrained the protein expression of the estrogen receptors a(eRa)and eRβ.Moreover,BA restored the balance of F-2 toxin-induced ovarian redox system ***,we found that 0.25 mg/kg BA played an anti-inflammatory role in the F-2 toxin-induced ovarian impairment by decreasing interleukin-1β(IL-1β).IL-6,and tumor necrosis factor-α(TNF-α)mRNA expression,as well as inhibiting p38 protein *** data demonstrated that BA exerts its protective effect on F-2 toxin-induced ovarian oxidative impairment and inflammation by inhibiting p38 expression,which implies a natural product-based medicine to ameliorate F-2 toxin-caused female reproductive toxicity and provides a detoxifying method for food contaminated by mycotoxin.

关键词： Betulinic acid F-2 toxin Ovarian damage p38 MAPK signaling pathway

FPR1 Antagonist (BOC-MLF) Inhibits Amniotic epithelial-mesenchymal Transition

在线全文

学校读者我要写书评

暂无评论

Current Medical Science 2024年第1期44卷 187-194页

作者： Xiao-mei huang e LIAO Jun-qun LIAO Ya-ling LIU Yong SHAO Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070China Medical Laboratory Science The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Yubei Maternity and Child Healthcare HospitalChongqing 400042China

Objective:Premature rupture of membranes(PROM)is a common pregnancy disorder that is closely associated with structural weakening of fetal *** have found that formyl peptide receptor 1(FPR1)activates inflammatory pathways and amniotic epithelial-mesenchymal transition(eMT),stimulates collagen degradation,and leads to membrane weakening and membrane *** purpose of this study was to investigate the anti-inflammatory and eMT inhibitory effects of FPR1 antagonist(BOC-MLF)to provide a basis for clinical prevention of ***:The relationship between PROM,FPR1,and eMT was analyzed in human fetal membrane tissue and plasma samples using Western blotting,PCR,Masson staining,and eLISA ***(LPS)was used to establish a fetal membrane inflammation model in pregnant rats,and BOC-MLF was used to treat the LPS rat *** detected interleukin(IL)-6 in blood from the rat hearts to determine whether the inflammatory model was successful and whether the anti-inflammatory treatment was *** used electron microscopy to analyze the structure and collagen expression of rat fetal ***:Western blotting,PCR and Masson staining indicated that the expression of FPR1 was significantly increased,the expression of collagen was decreased,and eMT appeared in *** rat model indicated that LPS caused the collapse of fetal membrane epithelial cells,increased intercellular gaps,and decreased ***-MLF promoted an increase in fetal membrane collagen,inhibited eMT,and reduced the weakening of fetal ***:The expression of FPR1 in the fetal membrane of PROM was significantly increased,and eMT of the amniotic membrane was ***-MLF can treat inflammation and inhibit amniotic eMT.

关键词： formyl peptide receptor 1 BOC-MLF epithelial-mesenchymal transition premature rupture of membranes

Stimulated Raman scattering tomography for rapid three-dimensional chemical imaging of cells and tissue

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Shock-induced energy localization and reaction growth considering chemical-inclusions effects for crystalline explosives

Advanced Photonics 2024年第2期6卷 20-31页

作者： Weiqi Wang Zhiwei huang National University of Singapore College of Design and EngineeringOptical Bioimaging LaboratoryDepartment of Biomedical EngineeringSingapore

Three-dimensional(3D)imaging is essential for understanding intricate biological and biomedical systems,yet live cell and tissue imaging applications still face challenges due to constrained imaging speed and strong scattering in turbid ***,we present a unique phase-modulated stimulated Raman scattering tomography(PM-SRST)technique to achieve rapid label-free 3D chemical imaging in cells and *** accomplish PM-SRST,we utilize a spatial light modulator to electronically manipulate the focused Stokes beam along the needle Bessel pump beam for SRS tomography without the need for mechanical z *** demonstrate the rapid 3D imaging capability of PM-SRST by real-time monitoring of 3D Brownian motion of polystyrene beads in water with 8.5 Hz volume rate,as well as the instant biochemical responses to acetic acid stimulants in MCF-7 ***,combining the Bessel pump beam with a longer wavelength Stokes beam(NIR-II window)provides a superior scattering resilient ability in PM-SRST,enabling rapid tomography in deeper tissue *** PM-SRST technique providestwofold enhancement in imaging depth in highly scattering media(e.g.,polymer beads phantom and biotissue like porcine skin and brain tissue)compared with conventional point-scan *** also demonstrate the rapid 3D imaging ability of PM-SRST by observing the dynamic diffusion and uptake processes of deuterium oxide molecules into plant *** rapid PM-SRST developed can be used to facilitate label-free 3D chemical imaging of metabolic activities and functional dynamic processes of drug delivery and therapeutics in live cells and tissue.

关键词： stimulated Raman scattering tomography deep tissue Raman imaging spatial light modulation

来源：评论

学校读者我要写书评

暂无评论

Defence Technology（防务技术） 2024年第3期33卷 278-294页

作者： Ruqin Liu Yanqing Wu Xinjie Wang Fenglei huang Xiaona huang Yushi Wen State Key Laboratory of explosion Science and Technology Beijing Institute of TechnologyBeijing100081China School of Power and Mechanical engineering Wuhan UniversityWuhan430072HubeiChina Institute of Chemical Materials China Academy of Engineering Physics(CAEP)Mianyang621999SichuanChina

Chemical inclusions significantly alter shock responses of crystalline explosives in macroscale gap experiments but their microscale dynamics origin remains *** shock-induced energy localization,overall physical responses,and reactions in a-1,3,5-trinitro-1,3,5-triazinane(a-RDX)crystal entrained various chemical inclusions were investigated by the multi-scale shock technique implemented in the reactive molecular dynamics *** indicated that energy localization and shock reaction were affected by the intrinsic factors within chemical inclusions,i.e.,phase states,chemical compositions,and *** atomic origin of chemical-inclusions effects on energy localization is dependent on the dynamics mechanism of interfacial molecules with free space volume,which includes homogeneous intermolecular compression,interfacial impact and shear,and void collapse and *** introducing various chemical inclusions,the initiation of those dynamics mechanisms triggers diverse decay rates of bulk RDX molecules and hereby impacts on growth speeds of final *** chemical inclusions can reduce the effectiveness of the void during the shock *** the shockwave velocity of 9 km/s,the parent RDX decay rate in RDX entrained amorphous carbon decreases the most and is about one fourth of that in RDX with a vacuum void,and solid HMX and TATB inclusions are more reactive than amorphous carbon but less reactive than dry air or acetone *** lessdense shocking system denotes the greater increases in local temperature and stress,the faster energy liberation,and the earlier final reaction into equilibrium,revealing more pronounced responses to the present intense *** quantitative models associated with the relative system density(RD_(sys))were proposed for indicating energy-localization mechanisms and evaluating initiation safety in the shocked crystalline ***_(sys)is defined by the density ratio of defective RDX to perfec

关键词： Shock responses energy localization Crystalline explosives Chemical inclusions Reactive molecular dynamics

Dynamic convergent shock compression initiated by return current in high-intensity laser-solid interactions

在线全文

学校读者我要写书评

暂无评论

Matter and Radiation at extremes 2024年第4期9卷 40-53页

作者： Long Yang Martin Rehwald Thomas Kluge Alejandro LasoGarcia Toma Toncian Karl Zeil Ulrich Schramm Thomas e.Cowan Lingen huang Helmholtz-Zentrum Dresden-Rossendorf 01328 DresdenGermany Technische Universitat Dresden 01062 DresdenGermany

We investigate the dynamics of convergent shock compression in solid cylindrical targets irradiated by an ultrafast relativistic laser *** particle-in-cell simulations and coupled hydrodynamic simulations reveal that the compression process is initiated by both magnetic pressure and surface ablation associated with a strong transient surface return current with density of the order of 10^(17) A/m^(2) and lifetime of 100 *** results show that the dominant compression mechanism is governed by the plasma β,i.e.,the ratio of thermal pressure to magnetic *** targets with small radius and low atomic number Z,the magnetic pressure is the dominant shock compression *** to a scaling law,as the target radius and Z increase,the surface ablation pressure becomes the main mechanism generating convergent ***,an indirect experimental indication of shocked hydrogen compression is provided by optical shadowgraphy measurements of the evolution of the plasma expansion *** results presented here provide a novel basis for the generation of extremely high pressures exceeding Gbar(100 TPa)to enable the investigation of high-pressure physics using femtosecond J-level laser pulses,offering an alternative to nanosecond kJ-laser pulse-driven and pulsed power Z-pinch compression methods.

关键词： shock convergent return

Machine learning-assisted sparse observation assimilation for real-time aerodynamic field perception

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2024年第5期67卷 1458-1469页

作者： ZHAO QingYu huang Jun GUO YuXin PAN YuXuan JI JingJing huang YongAn State Key Laboratory of Intelligent Manufacturing equipment and Technology School of Mechanical Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China

Accurate aerodynamic distribution perception and real-time flight state evaluation are crucial for flight safety,e.g.,stall ***,the observations are usually sparse due to limitations in sensor mounting space and cost,and a reconstruction technology is urgently ***,a machine learning-assisted assimilation method based on sparse observations has been *** from the traditional reconstruction methods focusing on boundary condition correction,the proposed method formulates the flow field pressure distribution as a linear superposition of flow field modes,thereby forming a real-time reconstruction pattern that combines offline modal extraction using computational fluid dynamics(CFD)with real-time determination of modal weights using a neural *** this study,CFD simulations were conducted under 800different operating conditions for common modal extraction and model *** weights of these modes were determined online based on merely five observations for reconstructing the full pressure field.A pressure reconstruction with a relative error of 6.1%and a mean square error of 0.003 was achieved within the prescribed condition *** computational cost was just2 ms for each reconstruction run,significantly faster than the 20 min required by the classical reconstruction ensemble transform Kalman *** also showed that the method maintains almost the same accuracy amidst 1.5%measurement *** practical examples,shock waves and the change of lift coefficient were analyzed using the proposed method,providing remarkable evidence for the capability of the method in supporting stall *** validate the method’s effectiveness and explore its potential in real-time and accurate monitoring of an aircraft.

关键词： aerodynamic force sparse observation neural networks pressure field reconstruction

In situ direct reprogramming of astrocytes to neurons via polypyrimidine tract-binding protein 1 knockdown in a mouse model of ischemic stroke

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第10期19卷 2240-2248页

作者： Meng Yuan Yao Tang Tianwen huang Lining Ke en huang Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Human Anatomy School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Scientific Research Center School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology Fujian Medical University Union HospitalFuzhouFujian ProvinceChina Fujian Key Laboratory of Vascular Aging Fujian Medical UniversityFuzhouFujian ProvinceChina

In situ direct reprogramming technology can directly convert endogenous glial cells into functional neurons in vivo for central nervous system repair. Polypyrimidine tract-binding protein 1(PTB) knockdown has been shown to reprogram astrocytes to functional neurons in situ. In this study, we used AAV-PHP.e B-GFAP-sh PTB to knockdown PTB in a mouse model of ischemic stroke induced by endothelin-1, and investigated the effects of GFAP-sh PTB-mediated direct reprogramming to neurons. Our results showed that in the mouse model of ischemic stroke, PTB knockdown effectively reprogrammed GFAP-positive cells to neurons in ischemic foci, restored neural tissue structure, reduced inflammatory response, and improved behavioral function. These findings validate the effectiveness of in situ transdifferentiation of astrocytes, and suggest that the approach may be a promising strategy for stroke treatment.

关键词： astrocyte in situ direct reprogramming ischemic stroke miR-30 based shRNA neuron polypyrimidine tract-binding protein 1 transdifferentiation