检索结果-南通市图书馆

Acta Pharmaceutica Sinica B 2019年第3期9卷 516-525页

作者： Hong zhang Liyan Huang Liyang Tao jianye zhang Fang Wang Xu zhang Liwu Fu Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangdong Esophageal Cancer InstituteGuangzhou510060China School of Pharmaceutical Sciences Guangzhou Medical UniversityGuangzhou511436China

Secalonic acid D(SAD) could inhibit cell growth in not only sensitive cells but also multidrug resistant(MDR) cells. However, the molecular mechanisms need to be elucidated. Here, we identified that SAD possessed potent cytotoxicity in 3 pairs of MDR and their parental sensitive cells including S1-MI-80 and S1,H460/MX20 and H460, MCF-7/ADR and MCF-7 cells. Furthermore, SAD induced cell G2/M phase arrest via the downregulation of cyclin B1 and the increase of CDC2 phosphorylation. Importantly, JNK pathway upregulated the expression of c-Jun in protein level and increased c-Jun phosphorylation induced by SAD, which was linked to cell apoptosis via c-Jun/Src/STAT3 pathway. To investigate the mechanisms of upregulation of c-Jun protein by SAD, the mR NA expression level and degradation of c-Jun were examined. We found that SAD did not alter the mR NA level of c-Jun but inhibited its proteasome-dependent degradation. Taken together, these results implicate that SAD induces cancer cell death through c-Jun/Src/STAT3 signaling axis by inhibiting the proteasome-dependent degradation of c-Jun in both sensitive cells and ATP-binding cassette transporter sub-family G member 2(ABCG2)-mediated MDR cells.

关键词： Multidrug resistance Secalonic acid D Apoptosis c-Jun ABCG2

成人急性主动脉夹层患者血管置换手术治疗后与住院死亡相关的危险因素分析

在线全文

学校读者我要写书评

暂无评论

中国急救医学 2024年第5期44卷 385-390页

作者：任建伟辛雨张建业温明修朱光发首都医科大学附属北京安贞医院呼吸与危重症医学科北京100029 首都医科大学附属北京安贞医院心脏内科中心北京100029 首都医科大学附属北京安贞医院心脏外科中心北京100029

目的探讨成人急性主动脉夹层(acute aortic dissection,AAD)患者血管置换手术治疗后与住院死亡相关的危险因素。方法连续纳入2018年1月至2023年12月首都医科大学附属北京安贞医院601例明确诊断为成人AAD患者(年龄≥18岁)的完整临床病历... 详细信息

目的探讨成人急性主动脉夹层(acute aortic dissection,AAD)患者血管置换手术治疗后与住院死亡相关的危险因素。方法连续纳入2018年1月至2023年12月首都医科大学附属北京安贞医院601例明确诊断为成人AAD患者(年龄≥18岁)的完整临床病历资料。根据住院结局分为生存组(n=532)和死亡组(n=69),单因素和多因素Logistic回归分析术前基线临床资料、术中和术后相关变量确定血管置换手术治疗后与住院死亡相关的独立危险因素。结果研究期间共601例成人AAD患者接受血管置换手术治疗,男性430例(71.5%),女性171例(28.5%),平均年龄(53.1±12.7)岁,平均体重指数(BMI)(25.6±4.1)kg/m^(2)。多因素Logistic回归分析显示,肾小球滤过率(estimated glomerular filtration rate,eGFR)(P0.05)。将AAD血管置换手术治疗后与住院死亡相关的独立危险因素纳入ROC曲线分析,结果显示,AUC为0.890(95%CI 0.848~0.932,P<0.001),敏感度为88.9%,特异度为68.3%。结论强调对高危人群的规范化围手术期管理,加强医院内感染防控措施,对降低成人AAD住院患者病死率及改善短期和长期预后具有重要意义。

关键词：急性主动脉夹层(AAD) 血管置换手术住院死亡院内感染危险因素

思政引领+能力导向的波谱解析“5E”教学模式思路探讨

在线全文

学校读者我要写书评

暂无评论

中国医药导报 2024年第13期21卷 53-56页

作者：孙明娜张建业陶移文广州医科大学药学院广东省分子靶标与临床药理学重点实验室广东广州511436

波谱解析是应用现代物理手段研究有机化合物结构的一门多学科交叉课程,应用范围广、实践性强。然而,该课程教学中存在学生学习兴趣低、吸收内化难度大、迁移应用训练少、能力价值提升慢等问题。在前期教学经验总结和学生学习效果反馈的... 详细信息

波谱解析是应用现代物理手段研究有机化合物结构的一门多学科交叉课程,应用范围广、实践性强。然而,该课程教学中存在学生学习兴趣低、吸收内化难度大、迁移应用训练少、能力价值提升慢等问题。在前期教学经验总结和学生学习效果反馈的基础上,作者开展将“5E”教学模式应用于药学波谱解析课程的探索研究。课前通过线上发布情景问题或热点案例吸引学生兴趣,引导其进行基础探究;课中进行线下深入探究、解释、知识迁移和初评价;课后通过线上作业/线下实践深化知识迁移并完善评价。“5E”教学模式有利于促进线上与线下的混合,加强理论与实践的结合,实现思政教育与课程的深度融合。

关键词：波谱解析 “5E”教学模式思政教育能力培养

The Impact of Irrigation on Bacterial Community Composition and Diversity in Liaohe Estuary Wetland

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2018年第4期17卷 855-863页

作者： LI Tiantian HU Hong LI Zhengyan zhang jianye LI Dong College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Colleget of Marine Life Sciences Ocean University of ChinaQingdao 26610Q China Institute of Wetland Science Panjin 124000China

In this study, the sequencing of 16S ribosomal DNA was used to characterize the soil bacterial community composition and diversity in Liaohe estuarine wetland. Soil samples were taken from different locations in the wetland dominated by reed. Moreover, the soil quality parameters were evaluated(p H, moisture, organic matter, total nitrogen, available nitrogen, total phosphorus, available phosphorus). The results showed that the organic matter and nutrient contents were significantly higher in irrigated wetland than those in natural wetland. Major phylogenic groups of bacteria in soil samples including Proteobacteria, Acidobacteria, Gemmatimonadetes, Actinobacteria and Cyanobacteria were analyzed and we found that Proteobacteria was the most abundant in the community, and the phylum Acidobacteria was more abundant in irrigated wetland. Beta diversity analyses indicated that the soil bacterial community was mainly affected by sampling sites rather than seasons. In general, the bacterial community in natural wetland was not significantly different with that in artificial irrigated wetland. Artificial hydraulic engineering irrigated according to the water requirement rule of reed, increased the production of reeds, changed the way of wetland soil material input, but the diversity of bacterial community kept stable relatively.

关键词： soil bacterial community Liaohe estuary wetland 16S rDNA sequencing nutrient

Euphorbia factor L2 induces apoptosis in A549 cells through the mitochondrial pathway

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2017年第1期7卷 59-64页

作者： Minting Lin Sili Tang Chao zhang Hubiao Chen Wenjing Huang Yun Liu jianye zhang School of Pharmaceutical Sciences Guangzhou Medical University School of Chinese Medicine Hong Kong Baptist University

Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed(the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced. We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species(ROS) generation,a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway. The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.

关键词： Euphorbia Factor L2 Caper euphorbia seed Euphorbia lathyris L Anticancer agent Apoptosis Mitochondrial pathway

骨化三醇对老年骨质疏松性脊柱骨折病人PKP术后骨折愈合的影响

在线全文

学校读者我要写书评

暂无评论

实用老年医学 2024年第3期38卷 240-244,250页

作者：叶恒张卫华韩俊张建业李永鸿武汉科技大学附属汉阳医院骨一科湖北省武汉市430050

目的研究骨化三醇用于老年骨质疏松性椎体压缩性骨折(OVCF)病人经皮椎体后凸成形术(PKP)后治疗对骨折愈合和血清骨碱性磷酸酶(BALP)、Ⅰ型前胶原N-端前肽(PINP)、胶原羧基端肽β特殊序列(β-CTX)表达水平的影响。方法纳入2019—2020年... 详细信息

目的研究骨化三醇用于老年骨质疏松性椎体压缩性骨折(OVCF)病人经皮椎体后凸成形术(PKP)后治疗对骨折愈合和血清骨碱性磷酸酶(BALP)、Ⅰ型前胶原N-端前肽(PINP)、胶原羧基端肽β特殊序列(β-CTX)表达水平的影响。方法纳入2019—2020年我院接受PKP手术的106例OVCF病人为研究对象,并采用随机数表法均分为观察组和对照组,每组53例。对照组PKP术后给予钙剂+阿仑膦酸钠片进行治疗,观察组在此基础上加用骨化三醇进行治疗,2组疗程均为6个月,比较2组临床疗效、Cobb角、Oswestry功能障碍指数(ODI)、骨密度和骨代谢指标的差异。结果2组治疗6个月有效率分别为90.57%和83.02%,差异无统计学意义(P>0.05),优良率分别为73.58%和52.83%,差异有统计学意义(P<0.05)。2组治疗1、3、6个月时Cobb角和ODI均明显低于治疗前(P<0.05),且观察组治疗6个月时ODI低于对照组,差异有统计学意义(P<0.05)。2组治疗3、6个月时骨密度均明显高于治疗前(P<0.05),且观察组治疗6个月时骨密度高于对照组,差异有统计学意义(P<0.05)。与治疗前相比,2组治疗1、3、6个月时BALP和PINP水平明显升高(P<0.05),β-CTX水平均明显降低(P<0.05),且观察组治疗1、3、6个月时BALP和PINP水平均高于对照组,差异有统计学意义(P<0.05)。结论OVCF病人PKP术后应用骨化三醇进行治疗具有良好效果,有利于改善骨代谢并增加骨密度,对促进骨折愈合、改善脊柱功能和提升日常生活能力具有积极作用。

关键词：骨质疏松性椎体压缩性骨折老年人经皮椎体后凸成形术骨化三醇骨碱性磷酸酶 Ⅰ型前胶原N-端前肽胶原羧基端肽β特殊序列

在线全文

学校读者我要写书评

暂无评论

面向新能源汽车的维修站改建评价方法

交通与运输 2024年第4期40卷 69-74页

作者：王扬张建业赖见辉李宇翔陈艳艳北京工业大学交通工程北京市重点实验室北京100124 北京交通大学经济管理学院北京100091 中和新兴能源研究院北京100162

在发展新能源汽车国家战略的驱动下,从改建现有燃油汽车维修站的角度出发,在兼顾燃油汽车维修需求的基础上,通过构建涵盖社会、环境、企业、政策、交通五大因素20个指标在内的改建评价指标体系,提出面向新能源汽车的TOPSIS-秩和比法维... 详细信息

在发展新能源汽车国家战略的驱动下,从改建现有燃油汽车维修站的角度出发,在兼顾燃油汽车维修需求的基础上,通过构建涵盖社会、环境、企业、政策、交通五大因素20个指标在内的改建评价指标体系,提出面向新能源汽车的TOPSIS-秩和比法维修站改建综合评价方法,对进一步完善维修站评价体系与方法起到积极的作用。同时,为我国汽车维修站转型改建发展提供了基础量化参考。最后,以北京市现有燃油汽车维修站改建为例,对提出方法的可行性进行了应用验证。评价结果表明,最适合改建的燃油汽车维修站主要集中在北京市中心城区,该区交通便利,人口密集,经济发达,有利于新能源汽车维修站的发展。分析建议政府可对相对薄弱的地区采取改建补贴政策、技术人才和维修资源引入等措施。北京市维修站在整体性提升的同时更需关注交通因素相关指标的均等化发展。

关键词：城市交通改建评价 TOPSIS-秩和比法维修站新能源汽车

“双三双四两结合”式药物化学混合式教学体系的建设与实践

在线全文

学校读者我要写书评

暂无评论

生物化工 2024年第3期10卷 138-141,151页

作者：吴文浩刘璨孙明娜季红王玉青张建业张超广州医科大学药学院广东广州511436

随着混合式教学的爆发式增长,自主学习效率低下,线上教学质量难以保障等问题显露无疑。本文针对性地将线上自主学习、远程学习支持、网络实时辅导与线下课堂教学、科学形成性评价、持续建设改进有机融合,通过教学内容重构,构建了教师“... 详细信息

随着混合式教学的爆发式增长,自主学习效率低下,线上教学质量难以保障等问题显露无疑。本文针对性地将线上自主学习、远程学习支持、网络实时辅导与线下课堂教学、科学形成性评价、持续建设改进有机融合,通过教学内容重构,构建了教师“讲、导、评”与学生“学、做、说”三位一体的“双三双四两结合”式药物化学新型混合式教学模式。实践证明,该模式能显著提高学生的学习积极性和参与度,教学质量能够得到有效的保证。

关键词：药物化学混合式教学教学模式教学改革课程思政

An New Computational Method of the Identification Jacobian for Robot Manipulator Calibraiton

在线全文

学校读者我要写书评

暂无评论

An New Computational Method of the Identification Jacobian f...

A Design of the Automatic Power-off after the Storage Battery of Electric Bicycle Is Fully Charged

2011 2nd Asia-Pacific Conference on Wearable Computing Systems

作者： jianye zhang School of Mechanical Engineering Tianjin University

Identification jacobian matrix, which represents the relationship between kinematic parameter error and the positional error of the robot manipulator, is very useful for the model based calibration of robot manipulator. In this paper, a new methodology to calculate the identification jacobian matrix for robot manipulator calibration is presented based on the modified Khalil and Kleinfinger notation. A computer program to calculate the identification jacobian matrix by using symbolic computation has been developed in MATLAB.

关键词： identification jacobian matrix robot manipulator computation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

International Journal of Technology Management 2015年第1期 56-58页

作者： jianye PENG Yi zhang The Center of Information Jiangsu University of Technology Changzhou 213001 Jiangsu China

As the service life of the electric bicycle＇ s storage battery is shortened due to the long-term floating charge, an automatic power- off circuit for the storage battery of electric bicycle is designed, and also the composition and design of the circuit are specifically expounded. After a test, the circuit can achieve a desired effect. Therefore, it can prolong the service life of the electric bicycle＇ s storage battery and save electric energy in the actual applications.

关键词： Electric Bicycle' s Storage Battery Voltage Comparison Circuit Relay