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A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2025年第2期20卷 503-517页

作者： Qi Liu jianye xie Runxue Zhou Jin Deng Weihong Nie Shuwei Sun Haiping Wang Chunying Shi Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdaoShandong ProvinceChina Department of Neurology The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Department of General Practice The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina

Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic ***,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in ***,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic *** metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ***,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel ***-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in *** results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor *** findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.

关键词： angiogenesis biomaterial blood-brain barrier cerebral ischemia/reperfusion injury control release drug delivery inflammation QK peptides matrix metalloproteinase-2 neuroprotection self-assembling nanofiber hydrogel

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T形钢管混凝土组合柱-钢筋混凝土梁外伸端板连接节点力学性能研究

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建筑结构 2016年第12期46卷 80-87页

作者：黄俊刘记雄蔡越谢建业戴绍斌武汉理工大学土木工程与建筑学院武汉430070 武汉理工大设计研究院武汉430070

通过静力加载试验及ABAQUS非线性有限元分析来研究T形钢管混凝土组合柱-钢筋混凝土梁外伸端板连接节点力学性能。结果表明:节点剪力主要由核心区形成的斜压杆来承担,而牛腿腹板承担的比例较小;设置加劲肋可有效抑制端板翘曲变形,缓解节... 详细信息

通过静力加载试验及ABAQUS非线性有限元分析来研究T形钢管混凝土组合柱-钢筋混凝土梁外伸端板连接节点力学性能。结果表明:节点剪力主要由核心区形成的斜压杆来承担,而牛腿腹板承担的比例较小;设置加劲肋可有效抑制端板翘曲变形,缓解节点区应力集中,比增加端板厚度更能有效提高节点抗弯承载力;轴压比小于0.4时,增大轴压比节点抗弯承载力略有提高,轴压比大于0.4时,增大轴压比节点抗弯承载力稍有降低,但提高、降低的幅值均很小;梁柱线刚度比对节点抗弯承载力影响较小;提高框架梁混凝土强度等级,节点抗弯承载力相应有所提高;提高框架梁配筋率,节点抗弯承载力明显增大,但当配筋率超过1.8%后继续提高时,节点抗弯承载力提高的幅值较小;有限元计算骨架曲线与试验结果能较好吻合,验证了有限元模型的合理性。

关键词： T形钢管混凝土组合柱外伸端板连接节点力学性能非线性有限元分析

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有机硝酸酯的大气化学及测量

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化学进展 2024年第8期36卷 1145-1156页

作者：邵豆秦敏方武韩宝彬解建业赵夏丹廖知堂胡嘉琪任恩伯中国科学院合肥物质科学研究院安徽光学精密机械研究所环境光学与技术重点实验室合肥230031 中国科学技术大学合肥230026

有机硝酸酯(ONs)是大气中重要的一类含氮有机物。大气中的ONs主要是在氮氧化物存在的条件下,由大气氧化剂和挥发性有机物的光化学反应二次生成,其生消过程与大气氮循环、臭氧以及二次有机气溶胶的形成息息相关;部分气相ONs还可通过气粒... 详细信息

有机硝酸酯(ONs)是大气中重要的一类含氮有机物。大气中的ONs主要是在氮氧化物存在的条件下,由大气氧化剂和挥发性有机物的光化学反应二次生成,其生消过程与大气氮循环、臭氧以及二次有机气溶胶的形成息息相关;部分气相ONs还可通过气粒分配或多次氧化进入颗粒相,形成颗粒相ONs,进而促进PM_(2.5)的生成,进一步危害人体健康。现阶段,多种化学及光谱学测量技术已成功应用于大气中ONs单物种和总量的浓度测量,并在不同区域开展了多种大气成分的联合外场观测,用于探究氮氧化物参与的ONs生成机制,ONs对区域氮化学的影响以及颗粒相ONs对二次有机气溶胶的贡献等。本文从ONs的生消机制指出其在大气化学中的重要作用,归纳总结了目前已有的ONs测量方法,并指出了不同方法的特点及应用,重点阐述了和ONs相关的室内烟雾箱研究和国内外不同地区的外场观测结果,结合大气化学模型对其在大气化学中的作用进行阐述,指出目前研究中存在的一些不足,以期在后续研究中不断得到完善。

关键词：有机硝酸酯大气化学测量技术外场观测

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保元汤合血府逐瘀汤治疗气虚血瘀型急性脑梗死临床研究

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陕西中医 2021年第5期42卷 590-593页

作者：董一王静云马君胡日光解建业青岛市胶州中心医院山东青岛266300 青岛大学附属医院山东青岛266003

目的:探究保元汤合血府逐瘀汤治疗气虚血瘀型急性脑梗死效果及对白细胞介素-33(IL-33)及视锥蛋白样蛋白-1(VILIP-1)的影响。方法:选取病例86例,按照随机数字表法分为对照组(采用常规方法进行治疗)和观察组(在对照组的基础上加用保元汤... 详细信息

目的:探究保元汤合血府逐瘀汤治疗气虚血瘀型急性脑梗死效果及对白细胞介素-33(IL-33)及视锥蛋白样蛋白-1(VILIP-1)的影响。方法:选取病例86例,按照随机数字表法分为对照组(采用常规方法进行治疗)和观察组(在对照组的基础上加用保元汤合血府逐瘀汤治疗),每组均为43例,两组均治疗1个月,观察两组治疗前后中医症候评分、观察两组治疗前后日常生活能力(ADL)、神经功能(NIHSS)、肢体运动功能(FMA)评分,观察两组治疗前后血清IL-33、VILIP-1水平,观察两组治疗后临床疗效以及不良反应发生情况。结果:治疗前,两组中医症候评分、ADL、NIHSS、FMA、NIHSS评分、血清IL-33、VILIP-1水平比较,差异无统计学意义(P>0.05);治疗后,两组中医症候评分、NIHSS评分、IL-33、VILIP-1水平均低于治疗前(P0.05)。结论:对气虚血瘀型急性脑梗死患者采用保元汤合血府逐瘀汤治疗,可显著改善患者的临床症状,调节血清IL-33及VILIP-1的表达,改善患者的日常生活能力、神经功能以及肢体运动功能。

关键词：急性脑梗死保元汤血府逐瘀汤气虚血瘀白细胞介素-33 视锥蛋白样蛋白-1

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Gene expression profiles in the peri-infarct brain cortex in a rat model of stroke-prone renovascular hypertension

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Neural Regeneration Research 2009年第11期4卷 857-861页

作者： Mei Li Yidong Wang Xiaogeng Shi Jingrui Pan Fen xie jianye Fang Department of Neurology SecondAffiliated Hospital of Sun Yat-sen University Guangzhou 510120 Guangdong Province China Department of Neurology Guangdong Hospital of Traditional Chinese Medicine Guangzhou 510120 Guangdong Province China

BACKGROUND： Previous studies have focused on gene expression acutely following stroke onset. However, there have been a few reports of gene expression during later stages of cerebral infarction. OBJECTIVE： To determine gene expression profiling in the peri-infarct brain cortex 7 days after ischemia in a rat model of cerebral infarction in renovascular hypertensive rats. DESIGN, TIME AND SETTING： An in vivo, molecular experiment was performed at the Experimental Animal Center of Sun Yat-sen University and CapitalBio, Beijing, China between February 2004 and August 2005. MATERIALS： A 70-mer oligo chip containing 5 705 rat genes was supplied by CapitalBio, Beijing, China; and the Oligo rat gene bank was provided by Qiagen, the Netherlands. METHODS： Six Sprague Dawiey rats were utilized to establish a stroke-prone renovascular hypertensive model using the two-kidney and two-clip method. The rats were subsequently randomly assigned to two groups： middle cerebral artery occlusion and sham-operation, with three rats in each group. The middle cerebral artery occlusion model was induced by intraluminal suture method. Incisions were sutured following isolation of carotid arteries in the sham-operation group. MAIN OUTCOME MEASURES： Total RNA was extracted from the peri-infarct cerebral cortex 7 days after surgery. Following fluorescent labeling, RNA was hybridized to an Oligo chip containing 5 705 genes and was then scanned. Images were collected and the differentially expressed genes （number and category） were selected by data analysis. RESULTS： A total of 174 genes were upregulated, and 23 were downregulated, in the peri-infarct cerebral cortex 7 days after ischemia. The upregulated genes were distributed among 12 functional categories, and the downregulated genes belonged to categories of transport, transcription regulators, signals, response to stress, metabolism, and cell adhesion. The expression of some cytoskeletal genes was upregulated, including VIM, A2M, B2M, ACTR3,

关键词： cerebral infarction renovascular hypertensive rat DNA microarray

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TMEM16A与肺动脉高压大鼠肺血管重构和增殖关系

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青岛大学学报（医学版） 2018年第3期54卷 296-301页

作者：刘文源解见业施春英贾秀娟孔庆暖章政青岛大学附属医院老年医学科山东青岛266003 青岛大学人体解剖与组织胚胎学系青岛市市立医院病理科

目的探讨肺动脉高压(PAH)大鼠肺血管重构、增殖与TMEM16A蛋白表达的关系。方法将30只8周龄雄性SD大鼠随机分为对照组、模型2周组和模型4周组,每组各10只。对照组单次腹腔注射体积分数为0.01的DMSO溶剂,模型组单次腹腔注射野百合碱50 mg... 详细信息

目的探讨肺动脉高压(PAH)大鼠肺血管重构、增殖与TMEM16A蛋白表达的关系。方法将30只8周龄雄性SD大鼠随机分为对照组、模型2周组和模型4周组,每组各10只。对照组单次腹腔注射体积分数为0.01的DMSO溶剂,模型组单次腹腔注射野百合碱50 mg/kg。应用小动物超声系统测定各组的肺加速时间(PAT)、射血时间(ET)、PAT/ET间接评估肺动脉压力,测定3组大鼠的右心室肥厚指数(RVHI),苏木精-伊红染色检测肺血管重塑等病理学指标,采用Western blot方法检测TMEM16A和PCNA蛋白的相对表达量,免疫荧光化学法检测各组肺血管平滑肌组织TMEM16A的表达。结果模型4周组PAT/ET较对照组明显降低(F=20.98,t=5.76,P<0.05)。对照组、模型2周组和模型4周组RVHI、血管横截面管壁面积与血管面积比值(WA%)和肺小动脉管壁厚度占血管直径的百分比(WT%)逐渐增高,且3组间RVHI、WT%、WA%比较差异均有统计学意义(F=166.60~234.26,t=4.37~21.03,P<0.05)。3组间TMEM16A和PCNA蛋白表达比较差异均有显著性(F=286.00、427.03,t=7.50~26.00,P<0.05)。对照组、模型2周组、模型4周组肺血管平滑肌组织TMEM16A的表达逐渐增加。3组的TMEM16A与WA%、PCNA,PCNA与WA%之间均呈正相关(r=0.901~0.969,P<0.05)。结论 TMEM16A表达水平均随造模时间延长而升高。TMEM16A表达与野百合碱诱导的PAH大鼠肺血管重构与增殖呈正相关,提示TMEM16A可能是PAH疾病进展中的重要蛋白。

关键词：高血压,肺性 TMEM16A 肺动脉血管重塑细胞增殖

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Dl-3-n butylphthalide attenuates brain injury caused by cortical infarction accompanied by cranial venous drainage disturbance

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Stroke & Vascular Neurology 2022年第3期7卷 222-236页

作者： Kangping Song Xiuli Zeng Xiaomei xie Rongxuan Zhu jianye Liang Guobing Chen Li’an Huang Department of Neurology Jinan University First Affiliated HospitalGuangzhouChina Medical Imaging Center Jinan University First Affiliated HospitalGuangzhouChina Institute of Geriatric Immunology Medical college of Jinan UniversityGuangzhouChina

Background Cerebral venous disorder may have a harmful effect on ischaemic stroke;however,the underlying mechanism remains to be *** Dl-3-n butylphthalide is a multitarget agent for antiischaemic stroke,its neuroprotective role in brain ischaemia accompanied by brain venous disturbance remains *** this study,we induced cerebral venous disturbance by the occlusion of bilateral external jugular veins(EJVs)to explore the potential mechanism of the adverse effects of cerebrovenous disorders in cerebral infarction and explore the protective effect of Dl-3-n butylphthalide on cerebral infarction accompanied through cerebral venous *** Cerebral venous disturbance was induced in Sprague-Dawley rats through the permanent occlusion of bilateral EJVs,and cerebral ischaemic stroke was induced through the permanent occlusion of the right cortical branches of the middle cerebral artery.2,3,5-triphenyltetrazolium chloride staining,MRI,Evans blue extravasation and behavioural test were performed to evaluate infarction volume,cerebral blood flow(CBF),blood-brain barrier(BBB)integrity and neurological *** staining and western blot analysis were performed to detect loss of neuron,endothelial cells,pericytes and tight *** Bilateral EJVs occlusion did not cause cerebral infarction;however,it increased the infarction volume compared with the simple middle cerebral artery occlusion(MCAO)group,accompanied by severe neuron loss,worse neurological function,lower CBF,increased EJVs pressure,exacerbated Evans blue extravasation and brain oedema,as well as attenuated ***-3-n butylphthalide displayed a neuroprotective effect in rats with MCAO accompanied by EJVs occlusion by reducing neuron loss,accelerating CBF restoration,promoting angiogenesis and relieving BBB *** Bilateral EJVs occlusion did not significantly affect normal rats but aggravated brain damage in the case of ischaemic ***

关键词： protective infarction disturbance

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Impacts of wind conditions on hydrogen leakage during refilling hydrogen-powered vehicles

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Energy Storage and Saving 2023年第2期2卷 449-458页

作者： Lin xie Yangyiming Rong jianye Chen Fang Yuan Rui Long School of Energy and Power Engineering Huazhong University of Science and TechnologyWuhan430074China Power China Huadong Engineering Corporation Limited Hangzhou311100China

Although hydrogen leakage at hydrogen refueling stations has been a concern,less efforts have been devoted to hydrogen leakage during the refueling of hydrogen-powered *** this study,hydrogen leakage and dilution from the hydrogen dispenser during the refueling of hydrogen-powered vehicles were numerically investigated under different wind *** shape,size,and distribution of flammable gas clouds(FGC)during the leakage and dilution processes were *** results showed that the presence of hydrogen-powered vehicles resulted in irregular FGC *** wind speeds(v wv)were associated with longer FGC propagation *** v_(wv)=2 m·s^(−1)and 10 m·s^(−1),the FGC lengths at the end of the leakage were 7.9 m and 20.4 m,*** downwind conditions,higher wind speeds corresponded to lower FGC *** FGC height was larger under upwind conditions and was slightly affected by the magnitude of the wind *** the dilution process,the existence of a region with a high hydrogen concentration led to the FGC volume first increasing and then gradually *** promoted the mixing of hydrogen and air,accelerated FGC dilution,inhibited hydrogen uplifting,and augmented the horizontal movement of the *** higher wind speeds,the low-altitude FGC movements could induce potential safety hazards.

关键词： Hydrogen safe Wind condition Hydrogen refilling Hydrogen leakage

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Carbon Doping Triggered Efficient Electrochemical Hydrogen Evolution of Cross-Linked Porous Ru-MoO_(2) Via Solid-Phase Reaction Strategy

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Energy & Environmental Materials 2023年第1期6卷 392-400页

作者： Jialin Cai jianye Yang Xin xie Jie Ding Leyan Liu Wanyu Tian Yushan Liu Zhiyong Tang Baozhong Liu Siyu Lu The Green Catalysis Center and College of ChemistryZhengzhou UniversityZhengzhou 450000China Henan Institute of Advanced Technology Zhengzhou UniversityZhengzhou 450000China National Center of Nanoscience and Technology Beijing 100190China College of Chemistry and Chemical Engineering Henan Polytechnic University2001 Century AvenueJiaozuo 454000China

The defect-free structure of Mo-based materials is a“double-edged sword”,which endows the material with excellent stability,but limits its chemical versatility and application in electrochemical hydrogen evolution reaction(HER).Carbon doping engineering is an attractive strategy to effectively improve the performance of Mo-based catalyst and maintain their ***,we report a cross-linked porous carbon-doped MoO_(2)(C–MoO_(2))-based catalyst Ru/C–MoO_(2) for electrochemical HER,which is prepared by the convenient redox solid-phase reaction(SPR)of porous RuO_(2)/Mo_(2)C composite *** studies reveal that due to the presence of carbon atoms,the electronic structure of C–MoO_(2) has been properly adjusted,and the loaded small Ru nanoparticles provide a fast water dissociation rate and moderate H adsorption *** electrochemical studies under a pH-universal environment,Ru/C–MoO_(2) electrocatalyst exhibits a low overpotential at a current density of 10 mA cm^(-2) and has a low Tafel ***,Ru/C-MoO_(2) has excellent stability for more than 100 h at an initial current density of 100 mA cm^(-2).

关键词： carbon doped hydrogen evolution reaction macro-meso-micropore MoO_(2) Ru solid-phase reaction

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Toward point-of-care assessment of patient response:a portable tool for rapidly assessing cancer drug efficacy using multifrequency impedance cytometry and supervised machine learning

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Microsystems & Nanoengineering 2019年第1期5卷 333-343页

作者： Karan Ahuja Gulam M.Rather Zhongtian Lin jianye Sui Pengfei xie Tuan Le Joseph R.Bertino Mehdi Javanmard Department of Electrical and Computer Engineering Rutgers UniversityNew BrunswickNJUSA Rutgers Cancer Institute of New Jersey Rutgers UniversityNew BrunswickNJUSA

We present a novel method to rapidly assess drug efficacy in targeted cancer therapy,where antineoplastic agents are conjugated to antibodies targeting surface markers on tumor *** have fabricated and characterized a device capable of rapidly assessing tumor cell sensitivity to drugs using multifrequency impedance spectroscopy in combination with supervised machine learning for enhanced classification *** commercially available devices for the automated analysis of cell viability are based on staining,which fundamentally limits the subsequent characterization of these cells as well as downstream molecular *** approach requires as little as 20μL of volume and avoids staining allowing for further downstream molecular *** the best of our knowledge,this manuscript presents the first comprehensive attempt to using high-dimensional data and supervised machine learning,particularly phase change spectra obtained from multi-frequency impedance cytometry as features for the support vector machine classifier,to assess viability of cells without staining or labelling.

关键词： assessing impedance drugs

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