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Post-surgical resection prognostic value of combined OPN, MMP7, and PSG9 plasma biomarkers in hepatocellular carcinoma

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Frontiers of Medicine 2019年第2期13卷 250-258页

作者： weiqi Rong Yang Zhang Lei Yang Lin Feng Baojun wei Fan Wu Liming Wang Yanning Gao Shujun Cheng jianxiong Wu Ting Xiao Department of Abdominal Surgery National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China State Key Laboratory of Molecular Oncology Department of Etiology and CarcinogenesisNational Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Clinical Laboratory National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Biomarkers for hepatocellular carcinoma (HCC) following curative resection are not currently sufficient for prognostic indication of overall survival (OS) and disease-free survival (DFS). The aim of this study was to investigate the prognostic performance of osteopontin (OPN), matrix metalloproteinase 7 (MMP7), and pregnancy specific glycoprotein 9 (PSG9) in patients with HCC. A total of 179 prospective patients with HCC provided plasma before hepatectomy. Plasma OPN, MMP7, and PSG9 levels were determined by enzyme-linked immunosorbent assay. Correlations between plasma levels, clinical parameters, and outcomes (OS and DFS) were overall analyzed. High OPN (≥149.97 ng/mL), MMP7 (≥2.28 ng/mL), and PSG9 (≥45.59 ng/mL) were prognostic indicators of reduced OS (P<0.001, P<0.001, and P=0.007, respectively). Plasma PSG9 protein level was an independent factor in predicting OS (P=0.008) and DFS (P=0.038). Plasma OPN+MMP7+PSG9 elevation in combination was a prognostic factor for OS (P<0.001). OPN was demonstrated to be a risk factor-associated OS in stage I patients with HCC and patients with low α-fetoprotein levels (<20 ng/mL). These findings suggested that OPN, MMP7, PSG9 and their combined panels may be useful for aiding in tumor recurrence and mortality risk prediction of patients with HCC, particularly in the early stage of HCC carcinogenesis.

关键词： biomarkers OPN MMP7 PSG9 HCC prognosis

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一个遗传性听神经病伴视神经萎缩家系研究

听力学及言语疾病杂志 2024年第2期32卷 107-111页

作者：董佩索利敏张磊何敏贾薇李通范林静李青峰杨洁靳玲李丹薛金梅赵长青张亚茜段建雄山西医科大学第二医院耳鼻咽喉头颈外科山西省气道炎性疾病神经免疫研究省级重点培育实验室太原030000 山西医科大学第二医院眼科山西医科大学基础医学院生物化学与分子生物学教研室山西国信凯尔医学检验所

目的研究探讨一个听神经病伴视神经萎缩家系遗传性致病原因。方法详细询问先证者病史及家族史、进行临床相关检查确诊听神经病伴视神经萎缩,绘制该家系遗传系谱。抽取先证者(Ⅲ-7)外周血行全外显子组测序,对检出的突变进行致病性判读... 详细信息

目的研究探讨一个听神经病伴视神经萎缩家系遗传性致病原因。方法详细询问先证者病史及家族史、进行临床相关检查确诊听神经病伴视神经萎缩,绘制该家系遗传系谱。抽取先证者(Ⅲ-7)外周血行全外显子组测序,对检出的突变进行致病性判读,对先证者妻子(Ⅲ-8)、大女儿(Ⅳ-7)、二女儿(Ⅳ-9)和儿子(Ⅳ-10)进行Sanger测序验证突变位点,并结合临床表现和检查结果进行研究。结果该家系遗传方式为常染色体显性遗传,先证者(Ⅲ-7)19岁时出现视力下降,30岁时出现双侧感音神经性聋,言语识别率下降,其所在5代20人大家系中10人(2人已故)有类似听力及视力下降症状。先证者(Ⅲ-7)、大女儿(Ⅳ-7)和儿子(Ⅳ-10)听力学检查:纯音测听示双侧感音神经性聋,ABR双耳未引出,40 Hz相关电位(AERP)双耳均未引出,OAE部分或全部频率可引出,镫骨肌声反射阈值未引出;Ⅲ-7、Ⅳ-10眼底检查有不同程度视乳头萎缩,OCT示双眼视盘神经纤维层厚度变薄、视觉诱发电位示P100波峰时延长,确诊为遗传性听神经病伴视神经萎缩。Ⅲ-7行全外显子检测发现3号染色体有一个致病位点OPA1基因c.1334G>A(***445His,NM_015560.2)突变,一代测序结果示Ⅳ-7和Ⅳ-10也有该突变,Ⅲ-8和Ⅳ-9该位点的基因型是野生纯合型,即未发生突变。结论 OPA1基因c.1334G>A(***445His,NM_015560.2)突变位点为该家系致病突变。

关键词：听神经病遗传性视神经萎缩 OPA1基因

体细胞克隆技术扩繁超细型二狼山绒山羊的应用研究

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中国畜牧兽医 2024年第4期51卷 1420-1427页

作者：卿玉波程雯角德灵刘城郝志强郭建雄李钰莹张筱琳李臻李效宇王文魏红江云南省小型猪基因编辑与异种器官移植重点实验室昆明650201 云南农业大学动物医学院昆明650201 云南农业大学动物科学技术学院昆明650201 内蒙古中科正标生物科技有限公司巴彦淖尔015000 内蒙古正标种畜有限公司巴彦淖尔015000 中国科学院昆明动物研究所昆明650201

【目的】通过体细胞克隆技术生产超细型二狼山绒山羊克隆个体,为该技术在地方优质羊品种资源扩繁及新品种培育中的应用提供理论依据。【方法】选择1只4岁龄羊绒细度为13.89μm的二狼山绒山羊(♂),采集耳组织并分离培养成纤维细胞,以其... 详细信息

【目的】通过体细胞克隆技术生产超细型二狼山绒山羊克隆个体,为该技术在地方优质羊品种资源扩繁及新品种培育中的应用提供理论依据。【方法】选择1只4岁龄羊绒细度为13.89μm的二狼山绒山羊(♂),采集耳组织并分离培养成纤维细胞,以其为供体细胞,利用体细胞克隆技术开展超细型二狼山绒山羊的扩繁研究,通过克隆羊体重、羊绒细度、繁殖性能等指标评估该技术的可行性。【结果】试验成功建立了稳定的山羊卵母细胞体外成熟培养及体细胞克隆技术体系,卵母细胞成熟率为44.8%,孤雌激活卵裂率、囊胚率分别为83.0%、22.4%;二狼山绒山羊克隆胚的卵裂率、囊胚率分别为77.8%、13.7%。获得5只克隆羊个体,其生长发育正常,二狼山绒山羊母羊自然交配共获得16只健康的F1代个体,具有正常繁殖能力。克隆个体羊绒细度极显著低于同龄对照个体(P<0.01)。【结论】本研究通过体细胞克隆技术成功克隆了超细型二狼山绒山羊个体,其具备正常生产性能和繁殖性能,并保留了超细羊绒的特性,为地方优良羊种质资源扩繁和新品种培育应用研究奠定了基础。

关键词：二狼山绒山羊体细胞克隆羊绒细度繁殖性能

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Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

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Bioactive Materials 2022年第12期7卷 300-320页

作者： Shuai wei Qian Hu jianxiong Ma Xiu Dai Yu Sun Gonghai Han Haoye Meng Wenjing Xu Lei Zhang Xinlong Ma Jiang Peng Yu Wang Tianjin Hospital Tianjin UniversityNo.406 Jiefang South RoadHexi DistrictTianjin300211China Institute of Orthopedics The First Medical CenterChinese PLA General HospitalBeijing Key Lab of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma and War Injuries PLANo.28 Fuxing RoadHaidian DistrictBeijing 100853China Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Neural Regeneration Co-Innovation Center of Jiangsu ProvinceNantong UniversityNo.19 Qixiu RoadChongchuan DistrictNantong226001China Department of Geriatrics The Second People’s Hospital of NantongAffiliated Rehabilitation Hospital of Nantong UniversityNo.298 Xinhua RoadChongchuan DistrictNantong226006China Institute of Orthopedics Tianjin Hospital Tianjin UniversityTianjin Key Laboratory of Orthopedic Biomechanics and Medical EngineeringNo.155 Munan RoadHeping DistrictTianjin300050China The First People’s Hospital of Yunnan Province No.157 Jinbi RoadXishan DistrictKunming650034China Academy of Medical Engineering and Translational Medicine Tianjin UniversityNo.92 Weijin RoadNankai DistrictTianjin300072China

Compared to conventional artificial nerve guide conduits (NGCs) prepared using natural polymers or synthetic polymers, acellular nerve grafts (ACNGs) derived from natural nerves with eliminated immune components have natural bionic advantages in composition and structure that polymer materials do not have. To further optimize the repair effect of ACNGs, in this study, we used a composite technology based on supercritical carbon dioxide (scCO_(2)) extraction to process the peripheral nerve of a large mammal, the Yorkshire pig, and obtained an innovative Acellular nerve xenografts (ANXs, namely, CD + scCO_(2) NG). After scCO_(2) extraction, the fat and DNA content in CD + scCO_(2) NG has been removed to the greatest extent, which can better supported cell adhesion and proliferation, inducing an extremely weak inflammatory response. Interestingly, the protein in the CD + scCO_(2) NG was primarily involved in signaling pathways related to axon guidance. Moreover, compared with the pure chemical decellularized nerve graft (CD NG), the DRG axons grew naturally on the CD + scCO_(2) NG membrane and extended long distances. In vivo studies further revealed that the regenerated nerve axons had basically crossed the CD + scCO_(2) NG 3 weeks after surgery. 12 weeks after surgery, CD + scCO_(2) NG was similar to autologous nerves in improving the quality of nerve regeneration, target muscle morphology and motor function recovery and was significantly better than hollow NGCs and CD NG. Therefore, we believe that the fully decellularized and fat-free porcine ACNGs may be the most promising “bridge” for repairing human nerve defects at this stage and for some time to come.

关键词： Peripheral nerve regeneration Acellular nerve xenograft Supercritical extraction Peripheral nerve injury Nerve defect

The Design and Simulation of the 2-D micro-carving parallel robot

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微计算机信息 2006年第1Z期22卷 221-223页

作者： (school of Mechanical and Electronic Engineering,Nanchang University,Nanchang 330029,China) Ye,jianxiong Zhang,Hua Tan,Manhua wei,Hui

A convenient and effective close loop control scheme, based on the analysis of the robot structure and its control link, has been proposed to satisfy the 2D mini-parallel-carve robot design in this paper. With the introduction of sequent deduction and reversed simulation in the conceiving phase, but more important, with the adoption of the proper restriction to reassure the exclusive result of coordinate transformation and movement planning, the equipment is designed and completed finally with satisfy conclusion.

关键词：机器人结构分析智能控制误差分析

An Outbreak of Dengue Virus Type 1--Jiangxi Province,China,2019

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China CDC weekly 2020年第35期2卷 684-687页

作者： Fuqiang Yang Guanghui Xia Zhili Zeng Zhongjian Li Huijian Cheng Daping Che Jun Zong jianxiong Li Tian Gong Guihua Liu weidong Zhou Bihai wei Xiaobo Liu Jiandong Li Sheng Ding Jiangxi Provincial Center for Disease Control and Prevention NanchangJiangxiChina Xin’gan County Center for Disease Control and Prevention Ji’anJiangxiChina Ji’an City Center for Disease Control and Prevention Ji’anJiangxiChina WHO Collaborating Centre for Vector Surveillance and Management National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Institute for Viral Disease Control and Prevention.Chinese Center for Disease Control and Prevention BeijingChina

What is known about this topic?Dengue fever is an acute febrile illness caused by four types of dengue virus(DENV 1–4),and is a mosquitoborne infectious *** incidence of dengue has increased dramatically around the world in recent *** estimated of 3.9 billion people in 128 countries are at risk of infection with dengue viruses,70%of whom are in *** 2017,the first local infections of dengue virus(DENV-2)in Jiangxi Province was reported in Zhanggong District,Ganzhou *** is added by this report?From August to September 2019,the first dengue outbreak happened in Xin’gan County,where 81 local dengue fever cases were reported,35 were laboratoryconfirmed cases,and 46 were clinically-diagnosed cases;all cases were dengue virus type *** DENV-virus strains isolated from the cases of Xin’gan County were close to the strains isolated in Singapore(MF033254|25657|Singapore|2016)and Henan(MK905537|He nan201903|China:Henan Province|2019).What are the implications for public health practice?The outbreak might have been caused by imported cases or covert *** outbreak in Xin’gan County indicated that more proactive countermeasures should be taken during the dengue epidemic period such as intensifying surveillance for dengue cases,virus serotype,and Aedes vector density and strengthening cooperation with customs and tourism ***,the outbreak should prompt training in medical institutions and improved ability for doctor’s to diagnose dengue fever,which is very important for early detection of dengue cases and taking preventive measures.

关键词： prompt strengthening Jiangxi

Li-Ca Alloy Composite Anode with Ant-Nest-Like Lithiophilic Channels in Carbon Cloth Enabling High-Performance Li Metal Batteries

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Research 2022年第3期2022卷 343-353页

作者： Zihao Wang Yuchi Liu jianxiong Xing Zhicui Song Aijun Zhou wei Zou Fu Zhou Jingze Li School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China Yangtze Delta Region Institute(Huzhou) University of Electronic Science and Technology of ChinaHuzhou 313001China Research and Development Center Tianqi Lithium Co.Ltd.Chengdu 610093China

Constructing a three-dimensional(3D)multifunctional hosting architecture and subsequent thermal infusion of molten Li to produce advanced Li composite is an effective strategy for stable Li metal ***,the pure liquid Li is difficult to spread across the surface of various substrates due to its large surface tension and poor wettability,hindering the production and application of Li composite ***,heteroatomic Ca is doped into molten Li to generate Li-Ca alloy,which greatly regulates the surface tension of the molten alloy and improves the wettability against carbon cloth(CC).Moreover,a secondary network composed of CaLi2 intermetallic compound with interconnected ant-nest-like lithiophilic channels is in situ formed and across the primary scaffold of CC matrix by infiltrating molten Li-Ca alloy into CC and then cooling treatment(LCAC),which has a larger and lithiophilic surface to enable uniform Li deposition into interior space of the hybrid scaffold without Li ***,LCAC exhibits a long-term lifespan for 1100 h under a current density of 5 mA cm^(-2)with fixed areal capacity of 5 mAh cm^(-2).Remarkably,full cells paired with practical-level LiFePO4 cathode of 2.45 mAh cm^(-2)deliver superior performance.

关键词： alloy intermetallic composite

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层序成因动力学参数类型及意义

地球学报 2005年第6期26卷 499-504页

作者：覃建雄韦跃龙成都理工大学地球科学院四川成都610059

层序地层学为沉积盆地分析的一个重要手段和方法。层序地层学在沉积盆地分析中的参数包括宏观参数和微观参数。宏观参数特指沉积盆地中同一成因类型或成因相关的层序构成的层序组合体的总体特征,主要包括物质组成、宏观轮廓及叠置序列... 详细信息

层序地层学为沉积盆地分析的一个重要手段和方法。层序地层学在沉积盆地分析中的参数包括宏观参数和微观参数。宏观参数特指沉积盆地中同一成因类型或成因相关的层序构成的层序组合体的总体特征,主要包括物质组成、宏观轮廓及叠置序列等。微观参数系指单个层序本身的识别标志,分为层序内部标志和层序界面标志。前者包括层序的几何形态、规模级别、内部构型、成因格架和充填序列等;后者包括界面的物理标识、剖面特征、成因属性和级别类型等。不同的层序参数类型揭示不同的成因背景,反映不同的动态过程和成因动力学意义。通过这些参数类型及其成因意义的研究,可分析层序形成与沉积盆地及板块构造的关系,进而研究层序的形成背景和成因动力学,最终为盆地动力学研究提供基础。

关键词：参数类型层序成因动力学沉积盆地分析

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乙醇DI/汽油PFI发动机性能与排放特性

内燃机学报 2019年第1期37卷 25-31页

作者：康瑞冯登全卫海桥华剑雄周磊天津大学内燃机燃烧学国家重点实验室天津300072

基于一台点燃式发动机,对缸内直喷(DI)乙醇和进气道喷射(PFI)汽油的复合喷射方式进行了研究.与传统喷油模式相比,采用乙醇-汽油复合喷射能够提升发动机动力性.随直喷乙醇比例增加,缸内爆发压力升高;受乙醇燃烧速率和缸内冷却效果的综合... 详细信息

基于一台点燃式发动机,对缸内直喷(DI)乙醇和进气道喷射(PFI)汽油的复合喷射方式进行了研究.与传统喷油模式相比,采用乙醇-汽油复合喷射能够提升发动机动力性.随直喷乙醇比例增加,缸内爆发压力升高;受乙醇燃烧速率和缸内冷却效果的综合影响,着火滞燃期和燃烧持续期先缩短后延长.最佳点火时刻下,单一汽油喷射(PFI和GDI)爆震频次超过10%,,发动机发生轻微爆震,而复合喷射乙醇比例超过20%,可消除爆震;随直喷乙醇比例增加,循环波动系数降低,当量燃油消耗率降低,指示热效率提高,复合喷射相对PFI可提高发动机热效率3.8%,;同时,能够有效降低NOx和HC常规气体排放物.通过采用相对较高的缸内直喷乙醇比例,复合喷射能够提高发动机热效率及抑制爆震并降低常规气体排放物.

关键词：点燃式发动机乙醇爆震常规气体排放物