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Recent advances in DNA glycosylase assays

Chinese Chemical Letters 2022年第8期33卷 3603-3612页

作者： Lili Wang Huige Zhang Wei Chen Hongli Chen jianxi xiao Xingguo Chen State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China

Genomic deoxyribonucleic acid(DNA)is selected as the ideal carrier for preserving and transmitting the genetic information over the course of ***,the genomic DNA is constantly exposed to various endogenous and environmental threats,causing a diversity of damaged bases,lesions,mismatches and base-pair modifications in the genome,eventually leading to genomic instability and *** excision repair(BER)is the most important repair mechanism,repairing a variety of DNA damages arising from oxidation,alkylation,methylation,deamination,and hydrolysis *** glycosylases are responsible for initiating the first step of the BER pathway through cleaving the N-glycosidic bond between the damaged base and the DNA ***,abnormal DNA glycosylases are associated with a variety of diseases such as cancer,cardiovascular disease,neurological disease and inflammation,suggesting the important role of DNA glycosylases in cancer diagnosis and ***,it is highly desirable to monitor the activity of DNA glycosylases,gaining a deep understanding of the restoration process of damaged DNA and clinical ***,a series of novel DNA glycosylases detection methods with excellent performance have been *** this minireview,we summarize the recent advances in DNA glycosylase assays including amplification-free assay and amplification-assisted ***,a brief introduction of amplification-free assay for DNA glycosylase is ***,amplification-assisted assays for DNA glycosylases are discussed in ***,the conclusion and prospects of the directions of DNA glycosylase assays are provided.

关键词： DNA glycosylases Base excision repair Polymerase chain reaction Isothermal signal amplification Nanomaterial-based biosensors

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Recent progresses of collagen dressings for chronic skin wound healing

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Collagen and Leather 2023年第4期5卷 23-43页

作者： Shuangni Shi Lili Wang Chen Song Linyan Yao jianxi xiao State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000GansuChina Gansu Engineering Research Center of Medical Collagen Lanzhou 730000GansuChina Department of Chemistry University of ConnecticutStorrsConnecticut 06269USA

The skin plays a fundamental role in regulating the body's internal balance and protecting against external traumas.A broad variety of environmental risk factors frequently result in acute skin wounds,whose inappropriate treatments would lead to chronic skin wounds that are difficult to *** dressings have been widely used to repair chronic skin wounds,however their drawbacks such as insufficient hemostatic efficacy and non-moist environment have severely limited their clinical *** the principal component of skin,collagen has always been a research hotspot in the field of chronic skin wounds due to its advantages of low antigenicity,high biocompatibility and superior ***-based dressings have been increasingly developed to heal the chronic wounds during the past decades,arising from their capability in decreasing protein and electrolyte losses in wound exudate,preventing bacterial contamination,permitting less painful dressing changes,and improving the healing *** review overviews recent progress of collagen dressings for chronic skin wound *** commonly used wound dressings for wound management have been first *** wound dressings have been categorized as films,sponges,hydrogels,nanofibers,and powders,and their efficacy has been *** critical functions of collagen dressings in wound healing,such as stopping bleeding,shortening inflammation,promoting angiogenesis,and stimulating tissue regeneration have been *** clinical applications of collagen dressings to repair different types of chronic wounds have been thoroughly summarized.A comprehensive list of commercialized collagen dressings has been updated,and an outlook of collagen dressings have been finally speculated.

关键词： Collagen Wound dressings Chronic skin wounds Wound healing

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Silicon quantum dots-based fluorescent sensor for the detection of cobalt with high sensitivity and selectivity

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Chinese Chemical Letters 2024年第1期35卷 451-455页

作者： Ebtihaj Mohammed Sullam Khalid Mohammed Adam Juanjuan Liu Hongli Chen jianxi xiao State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China Department of Chemistry Faculty of EducationUniversity of KordofanEl Obeid 51111Sudan

Fluorescent silicon quantum dots(Si QDs)were hydrothermally synthesized from a mixture of 3(2-aminoethylamino)propyl(dimethoxymethylsilane)(AEAPDMMS)and poly(vinylpyrrolidine)(PVP).The resulting Si QDs exhibited good water solubility and high *** the optimized conditions,the probe revealed an excellent linear fluorescence quenching effect on Co2+ranging from 1μmol/L to 120μmol/L with a limit of detection of 0.37μmol/L(based on 3 s/k).The quenching mechanism was studied,showing that static quenching(SQE)causes the main ***,the test paper based on Si QDs was prepared,which is cost-effective,high sensitivity,good selectivity,easy to use and show excellent anti-interference *** method was applied to analyze the content of Co2+in environmental water samples with satisfying results.

关键词： Silicon quantum dots Fluorescence Poly(vinylpyrrolidine) Test paper Cobalt detection

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Synthesis and Characterization of Fluorescent Silica Nanoparticles Functionalized with Collagens of Variable Lengths

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Chinese Journal of Chemistry 2016年第8期34卷 788-794页

作者： Shanshan Zhang Shenglong Ding Yong Qian Miaomiao Yuan Jun Lu Zaiman Liu jianxi xiao School of Chemical and Biological Engineering Lanzhou Jiaotong University Lanzhou Gansu 730070 China State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province College of Chemistry and Chemical Engineering Lanzhou University Lanzhou Gansu 730000 China Institute of Pathogenic Biology School of Basic Medical Sciences Lanzhou University Lanzhou Gansu 730000 China

Collagen, the most abundant protein in human body, has been widely used as an excellent natural material for diverse biomedical applications due to its superior properties such as ample biological interaction sites, minimal immunogenicity and high biocompatibility. Collagens of different lengths are produced by recombinant technology and utilized to functionalize fluorescent silica nanoparticles (FNPs). The collagen-functionalized FNPs display mono-disperse distribution, but their sizes are dependent on the length of collagen. These modified FNPs all show nice fluorescence profile as well as low cytotoxicity, suggesting promising applications in bioimaging. We have demonstrated that various types of collagen, conveniently produced by recombinant technology, can be used to modify silica nanoparticles with nice characteristics such as mono-dispersion, non-interference in fluorescence and low toxicity. It may endow fluorescent silica nanoparticles with broad biological applications.

关键词： collagen fluorescent silica nanoparticles recombinant technology functionalization

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Mg2+-Triggered and pH-Tuned in vitro Assembly of Trehalose-6-Phosphate Synthase

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Wuhan University Journal of Natural Sciences 2018年第5期23卷 396-402页

作者： ZHANG Shanshan YANG Fan ZHANG Yuping LIU Zaiman YU Linghui CHEN Ximing xiao jianxi College of Chemistry and Chemical Engineering Lanzhou University Lanzhou 730000 Gansu China School of Chemical and Biological Engineering Lanzhou Jiaotong University Lanzhou 730070 Gansu China School of Stomatology Lanzhou University Lanzhou 730000 Gansu China Key Laboratory of Desert & Desertification Cold & Arid Regions Environmental & Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 Gansu China

The enzyme Ots A（trehalose-P synthase） plays a critical role in the biosynthesis of trehalose, which is a nonreducing disaccharide that plays important functions in many organisms. By using light scattering technique, we discovered that Ots A in Arthrobacter strain A3 polymerized in the presence of divalent metal ions（Mg2＋ or Ca2＋）, and the kinetics of the assembly was dependent on their concentrations. We identified potential compounds that can affect the kinetics of the polymerization, particularly, heparin, which acts as a very promising inhibitor of the polymerization. The Ots A assembly turns out to be a very delicate process that is finely regulated by p H. Ots A may be in the polymerized form at physiological pH in vivo, suggesting a more complicated mechanism of the enzyme. These unique properties of Ots A provide novel insights into the molecular mechanism of the biosynthesis of trehalose.

关键词： OtsA(trehalose-P synthase) trehalose polymerization light scattering

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The least stable pathological collagen targeted antibody-mimetic-peptide monitors collagen lesions in tumor tissues

The least stable pathological collagen targeted antibody-mim...

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第十一届全国化学生物学学术会议

作者： Xiangdong Cai jianxi xiao State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceCollege of Chemistry and Chemical EngineeringLanzhou University

Collagen is the most important structural protein in the human body. It has shown that collagen lesion is a critical step in tumor invasion and metastasis. [1] It has been demonstrated that pathological collagen targeted antibody-mimetic-peptide（PCTP） is good at binding to denatured collagen, whereas conventional probes form a triple helix that loses capability. [2] Here, we reported that the least stable PCTP remained lasting single-chain and used to monitor the process of collagen lesions in tumors.

关键词： pathological collagen tumor tissue like-antibody peptide

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Self-Assembly of Charged Collagen Mimetic Peptide into Biofunctional Nanofiber

Self-Assembly of Charged Collagen Mimetic Peptide into Biofu...

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第十一届全国化学生物学学术会议

作者： Linyan Yao Jingyuan Yu Biyang Ling jianxi xiao State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceCollege of Chemistry and Chemical EngineeringLanzhou University

Collagen,the most abundant protein in the human body,which play an essential role in cell adhesion and growth. Since native collagen is difficult to modify and can engender pathogenic and immunological side effects. Collagen mimetic scaffolds play a pivotal role in regenerative medicine and tissue engineering. We have herein for the first time reported the design of charged collagen mimetic peptides(CMPs),that self-assemble into nanofiber,which well mimic the characteristic D-periodic architectural structure of native *** addition,the CMP conjugated with the integrin specific GFOGER sequence was shown to promote collagen mimetic cell adhesion and *** self-assembled peptide nanofiber was shown to have the ability to structurally and biologically mimic native collagen fiber,suggesting promising applications in tissue regeneration applications.

关键词： charged collagen mimetic peptide self-assembly nanofiber cell adhesion

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肿瘤特异性多肽探针及其在生物成像中的应用

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化学学报 2024年第3期82卷 367-376页

作者：王博蔡向东肖建喜兰州大学化学化工学院兰州730000 甘肃省医用胶原蛋白工程研究中心兰州730000

肿瘤是由细胞、细胞外基质及微环境等多因素组成的复杂系统,不同因素在肿瘤的发生和发展中发挥关键作用.肿瘤细胞、细胞外基质及微环境的特异性分析对于肿瘤的精准诊断和靶向治疗至关重要.多肽探针具有特异性高、生物相容性好、组织穿... 详细信息

肿瘤是由细胞、细胞外基质及微环境等多因素组成的复杂系统,不同因素在肿瘤的发生和发展中发挥关键作用.肿瘤细胞、细胞外基质及微环境的特异性分析对于肿瘤的精准诊断和靶向治疗至关重要.多肽探针具有特异性高、生物相容性好、组织穿透能力强和易于制备等优点,已被广泛用于肿瘤的特异性成像研究.本文综述了多肽探针对肿瘤细胞、免疫细胞等细胞靶点的特异性成像;并介绍了以胶原蛋白、纤维蛋白等外基质蛋白为靶点的肿瘤特异性多肽探针的成像研究.本文还总结了对肿瘤微环境中弱酸性、高酶活性等因素响应的肿瘤特异性多肽探针及其生物成像应用.最后,本文总结并讨论了肿瘤特异性多肽探针所面临的挑战与机遇,展望了其在肿瘤精准诊断和个性化治疗领域的前景.

关键词：肿瘤肿瘤微环境细胞外基质多肽探针生物成像

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靶向检测Cu^(2+)和S^(2-)的多肽探针及其在细胞和斑马鱼成像中的应用

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分析试验室 2023年第5期42卷 578-586页

作者：赵闯权思齐孙秀霞肖建喜兰州大学化学化工学院兰州730000 兰州大学药学院兰州730000

设计了一种新型多肽探针L(Dansyl-Gly-Ser-Ser-Gly),该多肽探针L单独存在时有强的荧光信号,Cu^(2+)的顺磁性淬灭传感机制可导致多肽探针L的荧光淬灭。因此探针L可以靶向检测Cu^(2+)而不受其他常见金属离子的干扰。滴定和Job工作曲线结... 详细信息

设计了一种新型多肽探针L(Dansyl-Gly-Ser-Ser-Gly),该多肽探针L单独存在时有强的荧光信号,Cu^(2+)的顺磁性淬灭传感机制可导致多肽探针L的荧光淬灭。因此探针L可以靶向检测Cu^(2+)而不受其他常见金属离子的干扰。滴定和Job工作曲线结果表明,L-Cu检测平台以摩尔比1∶1的方式结合,结合常数为8.60×10^(4)L/mol。计算机模拟结果表明,Cu^(2+)与多肽探针L中的2个丝氨酸上的氮原子结合。S^(2-)的加入使L-Cu检测平台中的多肽探针L游离出来,多肽探针L的荧光恢复。多肽探针L能够实现对Cu^(2+)和S^(2-)的靶向检测,并以“On-Off-On”模式响应,检出限分别为114 nmol/L和357 nmol/L。多肽探针L已成功用于L929细胞和斑马鱼中Cu^(2+)和S^(2-)的荧光成像,为人体内靶向检测Cu2+和S2-提供了一种新的机遇。

关键词：多肽探针 Cu^(2+) S^(2-) L929细胞斑马鱼

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新型重组人源弹性蛋白交联凝胶快速修复光损伤皮肤

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中国科学：化学 2023年第2期53卷 227-234页

作者：李佳楠孙秀霞肖建喜兰州大学化学化工学院兰州730000 兰州大学药学院兰州730000

光损伤是最常见的皮肤疾病,每年遭受皮肤光损伤的人次超过全球总人口的1/3.弹性蛋白是皮肤真皮层中的关键蛋白质,在维持皮肤形态中扮演着重要角色.弹性蛋白目前主要通过动物组织提取,它存在病毒传播隐患和容易降解等缺陷.本研究构建了... 详细信息

光损伤是最常见的皮肤疾病,每年遭受皮肤光损伤的人次超过全球总人口的1/3.弹性蛋白是皮肤真皮层中的关键蛋白质,在维持皮肤形态中扮演着重要角色.弹性蛋白目前主要通过动物组织提取,它存在病毒传播隐患和容易降解等缺陷.本研究构建了一种新型的重组人源弹性蛋白及其交联凝胶,能够快速修复光损伤皮肤.该重组人源弹性蛋白由人弹性蛋白片段组成,具有与天然弹性蛋白类似的二级结构和可逆相变特性.该重组人源弹性蛋白经THPC交联形成凝胶,具有良好的机械强度.扫描电子显微镜结果表明,该重组弹性蛋白凝胶具有均匀分布的网孔结构.细胞实验表明,该凝胶无细胞毒性,并可以显著促进成纤维细胞的增殖.光损伤模型小鼠的动物实验表明,该凝胶可减轻光损伤小鼠的炎症反应,有效抑制表皮异常增生,并明显缩短皮肤愈合周期.本研究开发的高纯度重组人源弹性蛋白及其交联凝胶,对光损伤皮肤有显著的修复效果,在皮肤医学领域具有广泛的应用前景.

关键词：重组人源弹性蛋白弹性蛋白交联凝胶紫外光损伤皮肤修复

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