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ZmcrtRB3 Encodes a Carotenoid Hydroxylase that Affects the Accumulation of α-carotene in Maize Kernel

Journal of Integrative Plant Biology 2012年第4期54卷 260-269页

作者： Yi Zhou Yingjia Han Zhigang li Yang Fu Zhiyuan Fu Shutu Xu jiansheng li Jianbing Yan Xiaohong Yang National Maize Improvement Center Key Laboratory of Crop Genomics and Genetic ImprovementChina Agricultural University100193 BeijingChina Zhengzhou Sub-center of Chinese National Maize Improvement Center Henan Agricultural UniversityZhengzhou 450002China National Key Laboratory of Crop Improvement Huazhong Agricultural UniversityWuhan 430070China

α-carotene is one of the important components of pro-vitamin A, which is able to be converted into vitamin A in the human body. One maize （Zea mays L.） ortholog of carotenoid hydroxylases in Arabidopsis thaliana, ZmcrtRB3, was cloned and its role in carotenoid hydrolyzations was addressed. ZmcrtRB3 was mapped in a quantitative trait locus （QTL） cluster for carotenoid-related traits on chromosome 2 （bin 2.03） in a recombinant inbred line （RIL） population derived from By804 and B73. Candidate-gene association analysis identified 18 polymorphic sites in ZmcrtRB3 significantly associated with one or more carotenoid-related traits in 126 diverse yellow maize inbred lines. These results indicate that the enzyme ZmcrtRB3 plays a role in hydrolyzing both α- andβ-carotenes, while polymorphisms in ZmcrtRB3 contributed more variation in α-carotene than that inβ-carotene. Two single nucleotide polymorphisms （SNPs）, SNP1343 in 5＇untranslated region and SNP2172 in the second intron, consistently had effects on or-carotene content and composition with explained phenotypic variations ranging from 8.7% to 34.8%. There was 1.7- to 3.7-fold change between the inferior and superior hapiotype for α-carotene content and composition. Thus, SNP1343 and SNP2172 are potential polymorphic sites to develop functional markers for applying marker-assisted selection in the improvement of pro-vitamin A carotenoids in maize kernels.

关键词： α-carotene association analysis maize pro-vitamin A ZmcrtRB3.

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Genome-wide association study of vitamin E in sweet corn kernels

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The Crop Journal 2020年第2期8卷 341-350页

作者： Yingni Xiao Yongtao Yu Gaoke li lihua Xie Xinbo Guo jiansheng li Yuliang li Jianguang Hu Crop Research Institute Guangdong Academy of Agricultural SciencesKey Laboratory of Crops Genetics and Improvement of Guangdong ProvinceGuangzhou 510640GuangdongChina School of Food Science and Engineering South China University of TechnologyGuangzhou 510641GuangdongChina National Maize Improvement Center of China Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Vitamin E,consisting of tocopherols and tocotrienols,serves as a lipid-soluble antioxidant in sweet corn kernels,providing nutrients to both plants and humans.Though the key genes involved in the vitamin E biosynthesis pathway have been identified in plants,the genetic architecture of vitamin E content in sweet corn kernels remains largely unclear.In the present study,an association panel of 204 inbred lines of sweet corn was constructed.Seven compounds of vitamin E were quantified in sweet corn kernels at 28 days after pollination.A total of 119 loci for vitamin E were identified using a genome-wide association study based on genotyping by sequencing,and a genetic network of vitamin E was constructed.Candidate genes identified were involved mainly in RNA regulation and protein metabolism.The known gene ZmVTE4,encodingγ-tocopherol methyltransferase,was significantly associated with four traits(α-tocopherol,α-tocotrienol,theα/γ-tocopherol ratio,and theα/γ-tocotrienol ratio).The effects of two causative markers on ZmVTE4 were validated by haplotype analysis.Finally,two elite cultivars(Yuetian 9 and Yuetian 22)with a 4.5-fold increase in the sum ofα-andγ-tocopherols were developed by marker-assisted selection,demonstrating the successful biofortification of sweet corn.Three genes(DAHPS,ADT2,and cmu2)involved in chorismate and tyrosine synthesis were significantly associated with theα/γ-tocotrienol ratio.These results shed light on the genetic architecture of vitamin E and may accelerate the nutritional improvement of sweet corn.

关键词： Sweet corn GBS Genome-wide association study Vitamin E Marker-assisted selection

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清华简“书”类文献的文本性质与墨家贤人观的扩充机制

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中原文化研究 2024年第2期12卷 122-128页

作者：李健胜湖南师范大学历史文化学院湖南长沙410081

清华简《程寤》《说命》《尹至》《尹诰》等“书”类文献蕴含着墨家“顺天志”“明鬼神”“尚贤”等思想观念,通过传“书”、改“书”、造“书”,墨家突出了上帝神鬼的现实政治功用,展现了独特的历史书写意识,这都能说明清华简“书”类... 详细信息

清华简《程寤》《说命》《尹至》《尹诰》等“书”类文献蕴含着墨家“顺天志”“明鬼神”“尚贤”等思想观念,通过传“书”、改“书”、造“书”,墨家突出了上帝神鬼的现实政治功用,展现了独特的历史书写意识,这都能说明清华简“书”类文献系墨家传本。墨家通过改作史实,把伊尹、傅说打造成出身卑微但功绩卓绝的贤人,借此将冲决贵族血缘关系合法性主张置于墨家化的历史故事之中。清华简《治邦之道》《治政之道》则反映出战国中后期墨家通过批判现实、选贤任能体制化及吸收其他诸子立论等方式,从理论建构层面进一步扩充了贤人观。

关键词：清华简 “书”类文献墨家贤人观

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清华简《子犯子余》《赵简子》所见反贪婪观念论析

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大庆师范学院学报 2024年第2期44卷 90-96页

作者：李健胜湖南师范大学历史文化学院湖南长沙410081

清华简《子犯子余》《赵简子》所见反对贪婪的观念源于对商周时期贪婪之举的批判、反思,简文从统治者个人修养与国家治理关系角度阐述了清廉执政的必要性,也强调君王将相的道德垂范作用。反贪婪观念的形成、发展与春秋时期反对利用权势... 详细信息

清华简《子犯子余》《赵简子》所见反对贪婪的观念源于对商周时期贪婪之举的批判、反思,简文从统治者个人修养与国家治理关系角度阐述了清廉执政的必要性,也强调君王将相的道德垂范作用。反贪婪观念的形成、发展与春秋时期反对利用权势来满足贪欲的立论方向相一致,并在战国诸子学派中得以延展、深化。综合简文内容、抄写年代及简文儒化现象等信息,清华简《子犯子余》《赵简子》所见反贪婪主张尚处于片段式观念阶段,不是成体系的反贪思想。

关键词：清华简《子犯子余》《赵简子》反贪婪观念

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一种带作弊识别的动态(t,n)门限量子秘密共享方案

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信息网络安全 2024年第5期24卷 745-755页

作者：郭建胜关飞婷李志慧陕西师范大学数学与统计学院西安710119

文章基于齐次线性反馈移位寄存器(linear Feedback Shift Register,LFSR)序列和d维单量子态提出了一种带作弊识别的动态(t,n)门限量子秘密共享方案。在该方案中,可信的分发者确定共享的秘密,并为参与者分配份额,参与者对传输中的粒子执... 详细信息

文章基于齐次线性反馈移位寄存器(linear Feedback Shift Register,LFSR)序列和d维单量子态提出了一种带作弊识别的动态(t,n)门限量子秘密共享方案。在该方案中,可信的分发者确定共享的秘密,并为参与者分配份额,参与者对传输中的粒子执行相应的酉操作来恢复秘密。在秘密重建阶段,通过执行量子态数字签名,不仅可以检测到作弊行为,还可以识别具体的作弊者并将其删除,最终参与者可以验证恢复秘密的正确性。该方案可以在不更改旧参与者份额的前提下,无需其他参与者的帮助便可实现参与者的动态更新。安全性分析表明,该方案能够抵抗常见的外部攻击和内部攻击。

关键词：量子秘密共享动态更新作弊识别量子态数字签名

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Randomized controlled multicenter clinical trial for integrated treatment of community-acquired pneumonia based on Traditional Chinese Medicine syndrome differentiation

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Journal of Traditional Chinese Medicine 2012年第4期32卷 554-560页

作者： jiansheng li Xueqing Yu Suyun li Haifeng Wang Yunping Bai Minghang Wang Zikai Sun Wei Zhang Zhaoshan Zhou Xianhua Jia Qingwei Zhou Department of Respiratory Medicine The First Affiliated Hospital of Henan College of Traditional Chinese Medicine the Geriatric Institute of Henan University of TCM Department of Respiratory Medicine The First Affiliated Hospital of Henan University of Traditional Chinese Medicine Jiangsu Provincial Hospital of TCM The Affiliated Hospital of Shandong University of TCM Qingdao Hospital of TCM

OBJECTIVE:To evaluate the efficacy and safety of treatment based on syndrome differentiation ofTraditional Chinese Medicine(TCM) for community-acquired pneumonia(CAP).METHODS:A total of 240 CAP patients were randomly divided into the following two groups:thecontrol group was treated by anti-infection plus conventional medicine treatment;and the trial group was treated by TCM plus the above-mentioned treatment given to the controls.The course of treatment was 14 days,and the patients were followed up for 7 days.RESULTS:Of the 240 patients,235 accomplished the whole process of treatment.The five patients who withdrew from the study were brought into an intent-to-treat analysis.The therapeutic effects of the trial group were superior to those of the control group(Plinically stable,with a higher score in the quality of life(Plity rate(P>0.05),white blood cell count(P>0.05),bacterial clearance rate(P>0.05),and adverse reactions between the two integrated groups.CONCLUSION:Treatment based on TCM syndrome differentiation for CAP has the advantages of resulting in less time to achieve a stable clinical condition,improvement of clinical symptoms and quality of life,and is comparatively safe.

关键词：综合治疗临床试验辨证施治中国医药基础随机肺炎社区

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Reuse of Fenton sludge as an iron source for NiFe_2O_4 synthesis and its application in the Fenton-based process

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Journal of Environmental Sciences 2017年第3期29卷 1-8页

作者： Hui Zhang Jianguo liu Changjin Ou Faheem Jinyou Shen Hongxia Yu Zhenhuan Jiao Weiqing Han Xiuyun Sun jiansheng li lianjun Wang Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse School of Environmental and Biological EngineeringNanjing Universityof Science and TechnologyNanjing 210094China Beijing Changping Water Authority Beijing 102200China

The potentially hazardous iron-containing sludge from the Fenton process requires proper treatment and disposal, which often results in high treatment cost. In this study, a novel method for the reuse of Fenton sludge as an iron source for the synthesis of nickel ferrite particles （NiFe204） is proposed. Through a co-precipitation method followed by sintering at 800℃, magnetic NiFe204 particles were successfully synthesized, which was confirmed by powder X-ray diffraction （XRD）, scanning electronic microscopy （SEM）, energy dispersive spectroscopy （EDS）, Fourier transform infrared spectroscopy （FT-IR） and Raman spectros- copy. The synthesized NiFe204 could be used as an efficient catalyst in the heterogeneous Fenton process. In phenol degradation with H202 or NiFe204 alone, the phenol removal efficiencies within the reaction time of 330 min were as low as 5.9% ±0.1% and 13.5% ± 0.4%, respectively. However, in the presence of both NiFe204 and H202, phenol removal efficiency as high as 95% ± 3.4% could be achieved, indicating the excellent catalytic performance of NiFe204 in the heterogeneous Fenton process. Notably, a rapid electron exchange between --NiH and --Fem ions in the NiFe204 structure could be beneficial for the Fenton reaction. In addition, the magnetic catalyst was relatively stable, highly active and recoverable, and has potential applications in the Fenton process for organic pollutant removal.

关键词： Fenton Iron-containing sludgeReuseNickel ferriteMagnetic catalyst

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多孔碳纤维电极材料的制备及电容去离子性能研究

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南京理工大学学报 2024年第1期48卷 104-111页

作者：张惠中田家宇李健生朱志高南京理工大学环境与生物工程学院江苏南京210094 哈尔滨工业大学环境学院黑龙江哈尔滨150001 河北工业大学土木与交通学院天津300401

由于碳纳米纤维比表面积(SSA)较低,在电容去离子(CDI)应用中,其脱盐效率并不理想。该研究采用静电纺丝技术制备了沸石咪唑酯骨架-8(ZIF-8)材料与聚丙烯腈(PAN)复合纳米纤维,并通过碳化工艺处理得到多孔碳纳米纤维电极材料。经过优化组... 详细信息

由于碳纳米纤维比表面积(SSA)较低,在电容去离子(CDI)应用中,其脱盐效率并不理想。该研究采用静电纺丝技术制备了沸石咪唑酯骨架-8(ZIF-8)材料与聚丙烯腈(PAN)复合纳米纤维,并通过碳化工艺处理得到多孔碳纳米纤维电极材料。经过优化组分及碳化工艺后,所得碳纤维电极的SSA和孔体积分别高达398.74 m^(2)/g和0.180 cm^(3)/g。在1.2 V和1.6 V电压下,该电极对500 mg/L的NaCl溶液(50 mL)的盐吸附容量分别达21.16 mg/g和39.04 mg/g。此外,电化学测试结果显示,提高碳纳米纤维电极的孔隙率可以在一定程度上提高双电层电容,从而实现更高的离子吸附量和更稳定的储能性能。

关键词：电容去离子吸附碳纳米纤维沸石咪唑酯骨架-8 静电纺丝

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Fault diagnosis of the bushing infrared images based on mask R‐CNN and improved PCNN joint algorithm

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High Voltage 2021年第1期6卷 116-124页

作者： Jun Jiang Yifan Bie jiansheng li Xiaoping Yang Guoming Ma Yuncai Lu Chaohai Zhang Jiangsu Key Laboratory of New Energy Generation and Power Conversion Nanjing University of Aeronautics and AstronauticsNanjingChina Department of Electrical&Electronic Engineering School of EngineeringThe University of ManchesterManchesterUK State Grid Jiangsu Electric Power Co.Ltd.Research Institute NanjingChina State Grid Jiangsu Electric Power Co.Ltd. NanjingChina State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources North China Electric Power UniversityBeijingChina

Bushings are served as an important component of the power transformers;it's of great significance to keep the bushings in good insulation condition.The infrared images of the bushing are proposed to diagnose the fault with the combination of image segmentation and deep learning,including object detection,fault region extraction,and fault diagnosis.By building an object detection system with the frame of Mask Region convolutional neural network(CNN),the bushing frame can be exactly extracted.To distinguish the fault region of bushings and the background,a simple linear iterative clustering‐based pulse coupled neural network is proposed to improve the fault region segmentation performance.Then,two infrared image feature parameters,the relative position and area,are explored to classify fault type effectively based on the K‐means cluster technique.With the proposed joint algorithm on bushing infrared images,the accuracy reaches 98%,compared with 44%by the conventional CNN classification method.The integrated algorithm provides a feasible and advantageous solution for the field application of bushing image‐based diagnosis.

关键词： neural fault algorithm

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Abiotic-Biological Hybrid Systems for CO2 Conversion to Value-Added Chemicals and Fuels

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Transactions of Tianjin University 2020年第4期26卷 237-247页

作者： jiansheng li Yao Tian Yinuo Zhou Yongchao Zong Nan Yang Mai Zhang Zhiqi Guo Hao Song Frontier Science Center for Synthetic Biology Key Laboratory of Systems Bioengineering(Ministry of Education)Tianjin 300072China School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China

Abiotic-biological hybrid systems that combine the advantages of abiotic catalysis and biotransformation for the conversion of carbon dioxide(CO2)to value-added chemicals and fuels have emerged as an appealing way to address the global energy and environmental crisis caused by increased CO2 emission.We illustrate the recent progress in this field.Here,we first review the natural CO2 fixation pathways for an in-depth understanding of the biological CO2 transformation strategy and why a sustainable feed of reducing power is important.Second,we review the recent progress in the construction of abiotic-biological hybrid systems for CO2 transformation from two aspects:(i)microbial electrosynthesis systems that utilize electricity to support whole-cell biological CO2 conversion to products of interest and(ii)photosynthetic semiconductor biohybrid systems that integrate semiconductor nanomaterials with CO2-fixing microorganisms to harness solar energy for biological CO2 transformation.Lastly,we discuss potential approaches for further improvement of abiotic-biological hybrid systems.

关键词： CO2 conversion Abiotic-biological hybrid systems Microbial electrosynthesis systems Photosynthetic semiconductor biohybrid systems

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