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HPTLC-fluorescent densitometry for screening aflatoxin B1 in millet and buckwheat

Food Science and Human Wellness 2024年

作者： Xudong Shi Xingjun Xi Yuetao Jia Zhijian Wang Jiawei guo Shiyao Wang Xiaoqian Tang Yisheng Chen College of Food Science and Engineering， Shanxi Agricultural University China National Institute of Standardization Key Laboratory of Detection for Mycotoxins， Ministry of Agriculture and Rural Affairs Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences

Given severe health-hazardous effects of aflatoxin B1 (AFB1) widely occurring in cereal grains and animal feeds， it is highly urgent to develop analytical methods for its rapid screening. In this work， we proposed a simple and high-throughput method for the determination of AFB1 in millet and buckwheat samples using high performance thin layer chromatography (HPTLC) linked to fluorescence densitometry. The first step was to optimize the solid-liquid extraction for the crude clean-up of the samples. The QuEChERS (Quick， Easy， Cheap， Effective， Robust and Safe) extraction strategy was used and different solvent systems for their extraction efficiency of AFB1 from the samples were evaluated. Then， trichloromethane/ethyl acetate (7/3， v/v) was used as the mobile phase to realize the separation of the targeted compound from background noises on silica gel plates. Quantification was readily performed with densitometry in fluorescence mode. In order to fix the optimal excitation wavelength， spectra scanning from 250-400 nm was carried out， revealing that 364 nm light gave the highest signal. With the optimized optical system， high sensitivity to AFB1 was achieved， with a limit of detection (LOD) at 3 μg/kg. Apart from that， good linearity (0.999) was obtained within the range of 1-80 ng/band of AFB1. To assess the analysis accuracy， two levels of AFB1 (50 and 100 μg/kg) were spiked into real grain samples. The obtained results showed that the recovery rates were within the range of (81.6–114.0)%. The proof-of-concept results of this work evidenced that HPTLC is a promising analytical tool for the screening of mycotoxin in difficult samples.

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High critical current density in Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12) electrolyte via interfacial engineering with complex hydride

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Rare Metals 2024年第2期43卷 692-701页

作者： Ying-Tong Lv Teng-Fei Zhang Zhao-Tong Hu Guang-Lin Xia Ze-Ya Huang Zhen-Hua Liu Li-Hua Que Cai-Ting Yuan Fang-Qin guo Takayuki Ichikawa Xue-Bin Yu College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing210016China Centre for Hydrogenergy College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing210016China Department of Materials Science Fudan UniversityShanghai200433China Graduate School of Advanced Science and Engineering Hiroshima University1-4-1 KagamiyamaHigashi-Hiroshima739-8527Japan

Garnet-type solid-state batteries(SSBs)are considered to be one of the most promising candidates to realize next-generation lithium metal batteries with high energy density and ***,the dendrite-induced short-circuit and the poor interfacial contact impeded the practical ***,interface engineering to achieve low interfacial resistance without high temperature calcination was developed,which Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO)was simply coated with complex hydride(Li_(4)(BH_(4))_(3)I(3L1L))in various mass ratios n(Li_(4)(BH_(4))_(3)I)-(100−n)LLZTO(10≤n≤40).The interfacial conductivity increases by more than three orders of magnitude from 8.29×10^(−6)S·cm^(−1)to 1.10×10^(−2)S·cm^(−1).Symmetric Li cells exhibit a high critical current density(CCD)of 4.0 mA·cm^(−2) and an excellent cycling stability for 200 h at 4.0 mA·cm^(−2).SSBs with polymeric sulfur-polyacrylonitrile(SPAN)cathode achieve a high discharge capacity of 1149 mAh·g^(−1) with a capacity retention of 91%after 100 cycles(0.2 C).This attempt guides a simple yet efficient strategy for obtaining a stable Li/LLZTO interface,which would promote the development of solid-state batteries.

关键词： Hydrides Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO) Critical current density Solid-state electrolytes(SSEs) Lithium-sulfur batteries

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Impact of undissociated volatile fatty acids on acidogenesis in a two-phase anaerobic system

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Journal of Environmental Sciences 2016年第4期28卷 196-201页

作者： Keke Xiao Yan Zhou Chenghong guo Yogananda Maspolim Wun Jern ng Advanced Environmental Biotechnology Centre Nanyang Environment and Water Research InstituteNanyang Technological University School of Civil and Environmental Engineering Nanyang Technological University

This study investigated the degradation and production of volatile fatty acids（VFAs）in the acidogenic phase reactor of a two-phase anaerobic system.20 mmol/L bromoethanesulfonic acid（BESA）was used to inhibit acidogenic methanogens（which were present in the acidogenic phase reactor）from degrading *** impact of undissociated volatile fatty acids（un VFAs）on＂net＂VFAs production in the acidogenic phase reactor was then evaluated,with the exclusion of concurrent VFAs degradation.＂Net＂VFAs production from glucose degradation was partially inhibited at high un VFAs concentrations,with 59%,37% and 60% reduction in production rates at 2190 mg chemical oxygen demand（COD）/L undissociated acetic acid（un HAc）,2130 mg COD/L undissociated propionic acid（un HPr）and 2280 mg COD/L undissociated n-butyric acid（un HBu）,*** profile of VFAs produced further indicated that while an un VFA can primarily affect its own formation,there were also un VFAs that affected the formation of other VFAs.

关键词： Anaerobic process Waste treatment Substrate inhibition Undissociated volatile fatty acids Acidogenesis

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Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing

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Journal of Environmental Sciences 2015年第10期27卷 121-129页

作者： Yogananda Maspolim Yan Zhou Chenghong guo Keke Xiao Wun Jern ng Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute (NEWRI)Nanyang Technological University (NTU) School of Civil and Environmental Engineering Nanyang Technological University (NTU)

2-Phase anaerobic digestion（AD）, where the acidogenic phase was operated at 2 day hydraulic retention time（HRT） and the methanogenic phase at 10 days HRT, had been evaluated to determine if it could provide higher organic reduction and methane production than the conventional single-stage AD（also operated at 12 days HRT）. 454 pyrosequencing was performed to determine and compare the microbial communities. The acidogenic reactor of the 2-phase system yielded a unique bacterial community of the lowest richness and diversity, while bacterial profiles of the methanogenic reactor closely followed the single-stage reactor. All reactors were predominated by hydrogenotrophic methanogens, mainly Methanolinea. Unusually, the acidogenic reactor contributed up to 24%of total methane production in the 2-phase system. This could be explained by the presence of Methanosarcina and Methanobrevibacter, and their activities could also help regulate reactor alkalinity during high loading conditions through carbon dioxide production. The enrichment of hydrolytic and acidogenic Porphyromonadaceae, Prevotellaceae, Ruminococcaceae and unclassified Bacteroidetes in the acidogenic reactor would have contributed to the improved sludge volatile solids degradation, and ultimately the overall 2-phase system＇s performance. Syntrophic acetogenic microorganisms were absent in the acidogenic reactor but present in the downstream methanogenic reactor, indicating the retention of various metabolic pathways also found in a single-stage system. The determination of key microorganisms further expands our understanding of the complex biological functions in AD process.

关键词： Sewage sludge 2-Phase anaerobic digestion Microbial community 454 pyrosequencing Methanogen

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脑钠肽、同型半胱氨酸测定在急性脑梗死诊治中的作用

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中国基层医药 2024年第6期31卷 869-873页

作者：郭琳新余钢铁集团有限公司中心医院神经内科新余338000

目的探讨血清脑钠肽(BNP)、同型半胱氨酸(Hcy)测定在急性脑梗死(ACI)患者临床诊治中的价值。方法采用病例对照研究,选取新余钢铁集团有限公司中心医院2021年1月至2022年6月收治的ACI患者205例为病例组,并根据其神经功能缺损评分(NIHSS)... 详细信息

目的探讨血清脑钠肽(BNP)、同型半胱氨酸(Hcy)测定在急性脑梗死(ACI)患者临床诊治中的价值。方法采用病例对照研究,选取新余钢铁集团有限公司中心医院2021年1月至2022年6月收治的ACI患者205例为病例组,并根据其神经功能缺损评分(NIHSS)将其分为轻度组(n=98)、中度组(n=69)及重度组(n=38);另选取该院同期健康体检的健康人120例为健康组。采集所有受试者空腹静脉血,测定血清BNP、Hcy,比较病例组中不同神经功能缺损程度亚组的Hcy、BNP水平。根据患者治疗后改良Rankin量表(mRS)评分将其分为预后良好组(mRS评分≤2分,n=142)、预后不良组(mRS评分>2分,n=63),比较不同预后患者血清Hcy、BNP水平,并采用Pearson相关性分析评估病例组BNP、Hcy水平与预后评分的相关性。结果病例组血清Hcy、BNP分别为(17.21±2.67)μmol/L、(279.56±47.40)ng/L,均显著高于健康组的(6.68±1.04)μmol/L、(57.38±8.56)ng/L,差异均有统计学意义(t=-41.38、-50.83,均Png/L,中度组分别为(17.63±3.95)μmol/L、(295.56±56.27)ng/L,轻度组分别为(12.35±2.24)μmol/L、(182.82±35.64)ng/L,三组差异均有统计学意义(F=197.54、248.20,均Png/L,均显著高于预后良好组,差异均有统计学意义(t=12.35、18.40,均P<0.001)。相关性分析显示,病例组血清BNP、Hcy水平与其mRS预后评分均呈正相关(r=0.796、0.780,均P<0.001)。结论ACI患者的血清Hcy、BNP水平,能有效评估其病情严重程度,且在预测其预后方面也能起到一定的参考作用。

关键词：脑梗死利钠肽,脑半胱氨酸诊断预后因素分析,统计学

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LDPE装置一次压缩机换热器中聚合物产生原因及解决措施

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合成树脂及塑料 2024年第2期41卷 55-58页

作者：郭钦生中国石油化工股份有限公司茂名分公司广东茂名525000

低密度聚乙烯装置一次压缩机出口换热器中会产生聚合物而出现堵塞现象。通过热重分析仪、差示扫描量热仪、凝胶渗透色谱仪表征了聚合物的分解温度、结晶性能和相对分子质量分布,并讨论了换热器堵塞的原因,提出了更换一次压缩机一段出口... 详细信息

低密度聚乙烯装置一次压缩机出口换热器中会产生聚合物而出现堵塞现象。通过热重分析仪、差示扫描量热仪、凝胶渗透色谱仪表征了聚合物的分解温度、结晶性能和相对分子质量分布,并讨论了换热器堵塞的原因,提出了更换一次压缩机一段出口故障疏油器、清理一次压缩机泄漏气管线、加大一次压缩机内部油注入量、保持换热器光洁等措施,保证了装置的平稳运行。

关键词：低密度聚乙烯一次压缩机换热器堵塞

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Analysis of a landfill cover without geomembrane using varied particle sizes of recycled concrete

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Journal of Rock Mechanics and Geotechnical Engineering 2023年第5期15卷 1263-1273页

作者： Charles Wang Wai ng Cheuk Lam ng Junjun Ni Haowen guo Qi Zhang Qiang Xue Rui Chen Department of Civil and Environmental Engineering Hong Kong University of Science and TechnologyHong KongChina School of Transportation Southeast UniversityNanjingChina State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China Department of Civil and Environmental Engineering Shenzhen Graduate SchoolHarbin Institute of TechnologyShenzhenChina

Previous studies have demonstrated the effectiveness of a novel three-layer landfill cover system constructed with recycled concrete aggregates(RCAs)without geomembrane in both laboratory and ***,no systematic investigation has been carried out to optimize the combination of the particle sizes for fine-grained RCAs(FRC)and coarse-grained RCAs(CRC)that can be used for the three-layer landfill cover *** aim of this paper is to assist engineers in designing the three-layer landfill cover system under a rainfall of 100-year return period in humid climate conditions using an easily controlled soil parameter D10 of *** numerical study reveals that when D10 of FRC increases from 0.05 mm to 0.16 mm,its saturated permeability increases by 10 *** a result,a larger amount of rainwater infiltrates into the cover system,causing a higher lateral diversion in both the top FRC and middle CRC *** further changes in the lateral diversion are observed when the D10 value of FRC is larger than 0.16 *** the particle sizes of FRC and CRC layers are shown to have a minor influence on the percolation under the extreme rainfall *** implies that the selection of particle sizes for the FRC and CRC layers can be based on the availability of *** it is well known that the bottom layer of the cover system should be constructed with very fine-grained soils if possible,this study provides an upper limit to the particle size that can be used in the bottom layer(D10 not larger than 0.02 mm).With this limit,the three-layer system can still minimize the water percolation to meet the design criterion(30 mm/yr)even under a 100-year return period of rainfall in humid climates.

关键词： Capillary barrier Landfill cover Particle size Recycled concrete

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人音猬因子相互作用蛋白在肿瘤中的分子调控机制及临床价值的研究进展

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复旦学报（医学版） 2024年第1期51卷 115-118页

作者：金安莉郭玮复旦大学附属中山医院检验科上海200032 复旦大学附属中山医院厦门医院检验科厦门361015

HHIP(hedgehog-interacting protein)基因编码人音猬因子相互作用蛋白,位于染色体4q31.21-31.3。作为Hedgehog(Hh)信号通路的内源性拮抗剂,HHIP能够抑制Hh信号通路活化。在人类肿瘤中,Hh信号通路通常呈异常激活状态,HHIP在大多数类型的... 详细信息

HHIP(hedgehog-interacting protein)基因编码人音猬因子相互作用蛋白,位于染色体4q31.21-31.3。作为Hedgehog(Hh)信号通路的内源性拮抗剂,HHIP能够抑制Hh信号通路活化。在人类肿瘤中,Hh信号通路通常呈异常激活状态,HHIP在大多数类型的肿瘤组织中普遍低表达。HHIP表达水平与肿瘤患者总生存期呈正相关,可作为肿瘤患者的独立预后标志物。本文从HHIP在肿瘤中的生物学功能、作用机制及其临床价值方面对HHIP研究进展作综述。

关键词：人音猬因子相互作用蛋白(HHIP) Hedgehog(Hh)信号通路肿瘤

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考虑加载次序影响的沥青非线性疲劳损伤累积研究

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工程力学 2024年第5期41卷 68-76页

作者：房辰泽冷真郭乃胜李辉蒋继望陆国阳王昊鹏大连海事大学交通运输工程学院辽宁大连116026 香港理工大学土木及环境工程学系中国香港九龙999077 诺丁汉大学诺丁汉交通工程中心英国诺丁汉NG72RD

变幅加载下沥青的疲劳损伤累积具有明显的非线性特征,传统的Miner’s线性疲劳损伤累积准则无法表征不同变幅加载次序下沥青的非线性疲劳损伤累积(NLFDA)。该研究旨在建立考虑加载次序影响的NLFDA模型,准确表征加载次序对沥青疲劳损伤累... 详细信息

变幅加载下沥青的疲劳损伤累积具有明显的非线性特征,传统的Miner’s线性疲劳损伤累积准则无法表征不同变幅加载次序下沥青的非线性疲劳损伤累积(NLFDA)。该研究旨在建立考虑加载次序影响的NLFDA模型,准确表征加载次序对沥青疲劳损伤累积的影响。通过开展应力控制的沥青恒幅加载疲劳试验,采用耗散伪应变能(DPSE)表征沥青疲劳损伤,分析恒幅加载下沥青的疲劳损伤累积规律;采用低-高和高-低两种加载次序,开展应力控制的沥青变幅加载疲劳试验,分析变幅加载下沥青的疲劳损伤累积规律;基于损伤等效准则,建立考虑加载次序影响的NLFDA模型,分析加载次序对疲劳损伤累积的影响。结果表明:应力控制模式下的沥青疲劳损伤,呈先缓慢后急剧的非线性增加演化趋势;恒幅加载下沥青疲劳损伤服从Miner’s准则发生线性累积,且累积寿命分数等于1;变幅加载下沥青疲劳损伤不服从Miner’s准则而发生非线性累积。低-高和高-低变幅加载次序下,沥青累积疲劳寿命随一级寿命分数的增大而分别增加和减小,累积寿命分数分别大于1和小于1;建立的NLFDA模型可克服Miner’s准则缺陷,并较为准确地表征加载次序对沥青疲劳损伤累积的影响。

关键词：道路工程沥青材料非线性疲劳损伤累积加载次序耗散伪应变能疲劳寿命

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Cumulus-specific genes are transcriptionally silent following somatic cell nuclear transfer in a mouse model

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第8期8卷 533-539页

作者： TOng guo-qing HEng Boon-chin ng Soon-chye Nuclear Reprogramming and Stem Cell Laboratory Department of Obstetrics and Gynecology Yong Loo Lin School of Medicine National University of Singapore 119074 Singapore Stem Cell and Developmental Biology Group Genome Institute of Singapore 138672 Singapore Stem Cell Laboratory Faculty of Dentistry National University of Singapore 119074 Singapore Embryonics International Pte Ltd. Gleneagles Hospital 258500 Singapore

This study investigated whether four cumulus-specific genes: follicular stimulating hormone receptor (FSHr), hyaluronan synthase 2 (Has2), prostaglandin synthase 2 (Ptgs2) and steroidogenic acute regulator protein (Star), were correctly reprogrammed to be transcriptionally silent following somatic cell nuclear transfer (SCNT) in a murine model. Cumulus cells of C57×CBA F1 female mouse were injected into enucleated oocytes, followed by activation in 10 μmol/L strontium chloride for 5 h and subsequent in vitro culture up to the blastocyst stage. Expression of cumulus-specific genes in SCNT-derived embryos at 2-cell, 4-cell and day 4.5 blastocyst stages was compared with corresponding in vivo fertilized embryos by real-time PCR. It was demonstrated that immediately after the first cell cycle, SCNT-derived 2-cell stage embryos did not express all four cumulus-specific genes, which continually remained silent at the 4-cell and blastocyst stages. It is therefore concluded that all four cumulus-specific genes were correctly reprogrammed to be silent following nuclear transfer with cumulus donor cells in the mouse model. This would imply that the poor preimplantation developmental competence of SCNT embryos derived from cumulus cells is due to incomplete reprogramming of other embryonic genes, rather than cumulus-specific genes.

关键词： Somatic cell nuclear transfer (SCNT) Nuclear reprogramming Embryo Development

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