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Advancements in life-on-a-chip: The impact of “Beyond Limits Manufacturing” technology

Chinese Chemical Letters 2024年第5期35卷 13-16页

作者： Weiwei He Hongbo Zhang Xudong Lin Lili Zhu Tingting Zheng Hao Pei Yang Tian Min Zhang Guoyue Shi Lei Wu jianlong zhao Gulinuer Wumaier Shengqing Li Yufang Xu Honglin Li Xuhong Qian Shanghai Key Laboratory of New Drug Design School of PharmacyEast China University of Science and TechnologyShanghai 200237China School of Mechanical and Power Engineering East China University of Science and TechnologyShanghai 200237China Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument School of Biomedical EngineeringShenzhen Campus of Sun Yat-sen UniversityShenzhen 518000China Shanghai Key Laboratory of Green Chemistry and Chemical Processes Department of ChemistrySchool of Chemistry and Molecular EngineeringEast China Normal UniversityShanghai 200241China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 200050China Department of Pulmonary and Critical Care Medicine Huashan HospitalFudan UniversityShanghai 200040China Innovation Center for AI and Drug Discovery East China Normal UniversityShanghai 200062China Lingang Laboratory Shanghai 200031China

This review explores the concept of life-on-a-chip,which involves the creation of miniaturized biological systems,such as organs,tissues,and model organisms,on microscale platforms called microfluidic chips.These chips consist of intricately etched channels,wells,and chambers that enable precise control and observation of fluids,cells,and biochemical reactions,facilitating the simulation of various aspects of human or animal physiology and the study of responses to different stimuli,drugs,or disease conditions.The review highlights the application of a novel technology,“Beyond Limit Manufacturing”(BLM),in the development of sophisticated three-dimensional cell models and model organism microchips.Modelorganism-on-a-chip and organ-on-a-chip(OoC)are among the thriving developments in the field of microfluidics,allowing for the reconstruction of living microenvironments and implementation of multiple stimuli.The review discusses the latest advancements in life-on-a-chip technology using BLM and outlines potential future research directions,emphasizing the significant role of these chips in studying complex biological processes in a controlled and scalable manner.

关键词： Beyond Limit Manufacturing Model-organism-on-a-chip Organ-on-a-chip

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Attention-Based CNN Fusion Model for Emotion Recognition During Walking Using Discrete Wavelet Transform on EEG and Inertial Signals

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Big Data Mining and Analytics 2024年第1期7卷 188-204页

作者： Yan zhao Ming Guo Xiangyong Chen Jianqiang Sun jianlong Qiu School of Communication Engineering Hangzhou Dianzi UniversityHangzhou 310018China School of Automation and Electrical Engineering Linyi UniversityLinyi 276000China

Walking as a unique biometric tool conveys important information for emotion recognition.Individuals in different emotional states exhibit distinct walking patterns.For this purpose,this paper proposes a novel approach to recognizing emotion during walking using electroencephalogram(EEG)and inertial signals.Accurate recognition of emotion is achieved by training in an end-to-end deep learning fashion and taking into account multi-modal fusion.Subjects wear virtual reality head-mounted display(VR-HMD)equipment to immerse in strong emotions during walking.VR environment shows excellent imitation and experience ability,which plays an important role in awakening and changing emotions.In addition,the multi-modal signals acquired from EEG and inertial sensors are separately represented as virtual emotion images by discrete wavelet transform(DWT).These serve as input to the attention-based convolutional neural network(CNN)fusion model.The designed network structure is simple and lightweight while integrating the channel attention mechanism to extract and enhance features.To effectively improve the performance of the recognition system,the proposed decision fusion algorithm combines Critic method and majority voting strategy to determine the weight values that affect the final decision results.An investigation is made on the effect of diverse mother wavelet types and wavelet decomposition levels on model performance which indicates that the 2.2-order reverse biorthogonal(rbio2.2)wavelet with two-level decomposition has the best recognition performance.Comparative experiment results show that the proposed method outperforms other existing state-of-the-art works with an accuracy of 98.73%.

关键词： walking multi-modal fusion virtual reality emotion recognition discrete wavelet transform attention mechanism

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Electricity resonance-induced phase transition of water confined in nanochannels

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Communications in Theoretical Physics 2024年第5期76卷 159-165页

作者： Yunqiu Ma Zhigao zhao Shaoqian Hao jianlong Kou Jiang-Xing Chen Institute of Condensed Matter Physics Zhejiang Institute of Photoelectronics&Zhejiang Institute for Advanced Light SourceZhejiang Normal UniversityJinhua 321004China Department of Physics Inner Mongolia UniversityHohhot 010021China Department of Physics Hangzhou Normal UniversityHangzhou 311121China

The phase transition of water molecules in nanochannels under varying external electric fields is studied by molecular dynamics simulations.It is found that the phase transition of water molecules in nanochannels occurs by changing the frequency of the varying electric field.Water molecules maintain the ice phase when the frequency of the varying electric field is less than 16 THz or greater than 30 THz,and they completely melt when the frequency of the varying electric field is 24 THz.This phenomenon is attributed to the breaking of hydrogen bonds when the frequency of the varying electric field is close to their inherent resonant frequency.Moreover,the study demonstrates that the critical frequency varies with the confinement situation.The new mechanism of regulating the phase transition of water molecules in nanochannels revealed in this study provides a perspective for further understanding of the phase transition of water molecules in nanochannels,and has great application potential in preventing icing and deicing.

关键词： electricity resonance phase transition water molecules molecular dynamics simulations

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Droplet microfluidic chip for precise monitoring of dynamic solution changes

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纳米技术与精密工程(英文) 2023年第3期6卷 55-63页

作者： Cong Ma Zehang Gao jianlong zhao Shilun Feng School of Information Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Department of Clinical Laboratory Third Affiliated Hospital of Guangzhou Medical UniversityGuangdong 510150China

In this work,an automated microfluidic chip that uses negative pressure to sample and analyze solutions with high temporal resolution was developed.The chip has a T-shaped channel for mixing the sample with a fluorescent indicator,a flow-focusing channel for generating droplets in oil,and a long storage channel for incubating and detecting the droplets.By monitoring the fluorescence intensity of the droplets,the device could detect changes in solution accurately over time.The chip can generate droplets at frequencies of up to 42 Hz with a mixing ratio of 1:1 and a temporal resolution of 3–6 s.It had excellent linearity in detecting fluorescein solution in the concentration range 1–5μM.This droplet microfluidic chip provides several advantages over traditional methods,including high temporal resolution,stable droplet generation,and faster flow rates.This approach could be applied to monitoring calcium ions with a dynamic range from 102 to 107 nM and a detection limit of 10 nM.

关键词： Microfluidic chip Droplet sampling Fluorescence detection Calcium ion dynamics Temporal resolution

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Molecular cloning and functional analysis of the pepper resistance gene Me3 to root-knot nematode

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Horticultural Plant Journal 2023年第1期9卷 133-144页

作者： Yang Liu Hongyi Cao Jian Ling Yuhong Yang Yan Li Bingyan Xie jianlong zhao Zhenchuan Mao Institute of Vegetables and Flowers Chinese Academy of Agricultural ScienceBeijing 100081China

Root-knot nematodes(RKNs)cause severe diseases in peppers annually around the world.In pepper,the Me3 gene provides a heat-stable and broad-spectrum resistance to RKNs.In this study,several simple sequence repeat(SSR)markers and insertion/deletion(In Del)markers were developed to fine map the Me3 gene.Analysis of 2272 individuals(F2progenies)revealed that Me3 was located in a 45-kb DNA region between markers SSR784 and SSR339,in which there were three candidate genes.Among them,as a novel nucleotide binding site and leucine rich repeat(NBS-LRR)family gene,the DNA sequence of Capana09g000163 of pepper line‘HDA149’was 6348 bp in length,with a 2802-bp open reading frame encoding 933 amino acids,including NB-ARC and LRR domains.Tobacco transient expression assays demonstrated that expression of Capana09g000163 triggered a hypersensitive response(HR)in Nicotiana benthamiana leaves.Subcellular localization results showed that the Capana09g000163 protein was localized in the cell nucleus.Ectopic expression of Capana09g000163 in Arabidopsis significantly increased resistance against Meloidogyne incognita compared with the wild-type(WT)Arabidopsis.Furthermore,M.incognita was almost unable to develop in transgenic Arabidopsis expressing Capana09g000163.Taken together,we cloned the Me3 gene and verified that it induced resistance against M.incognita with the methods of map-based cloning and transgenic technology,which may be of great significance to pepper breeding for resistance against RKNs.

关键词： Capsicum annuum L Meloidogyne incognita Nematode-resistant gene Fine mapping Transgenic technology

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Single-cell level point mutation analysis of circulating tumor cells through droplet microfluidics

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Chinese Chemical Letters 2022年第5期33卷 2701-2704页

作者： Shihui Qiu Chuanjie Shen Xiaoyu Jian Yunxing Lu zhaoduo Tong Zhenhua Wu Hongju Mao jianlong zhao State Key State Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Tumor heterogeneity plays a critical role in the determination of appropriate anticancer therapy.As cir-culating tumor cells(CTCs)contain all tumor-related information,the genetic changes on CTCs could help us choose the appropriate treatments for different patients.Single-base mutations are very common in tumor genetic changes which may result in drug resistance.Here,we introduce a single-cell mutation de-tection platform based on droplet microfluidics.This platform integrates cell capsulation,cell lysis,poly-merase chain reaction(PCR)and the observation process.The droplets’generation speed is over 6000 per minute and more than 600 cells could be encapsulated in one second.To verify the performance of our platform in practical use,we performed the mutation analysis of 4 kinds of cells with our platform and noted that the genetic status of each single cell was clearly discriminated.Moreover,these results agreed with those from direct sequencing.Compared with other forms of single-cell mutation detection techniques,our platform has high throughput,short experimental time and less experimental operations.

关键词： Single cell CTCs Point mutation EGFR Microfluidics

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Chip-based visual detection of micro RNA using DNA-functionalized gold nanoparticles

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Science China(Life Sciences) 2016年第5期59卷 510-515页

作者： Ping Wang jianlong zhao Bin Hu Zule Cheng Yanan Bai Qinghui Jin Huiying Liu Hongju Mao Sanqiang Li jianlong zhao The Molecular Medicine Key Laboratory of Liver Injury and Repair Medical College Henan University of Science and Technology State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Clinical Research Center of Biological Sensing Technology Shanghai Xuhui Central Hospital University of Chinese Academy of Sciences Department of Oral and Maxillofacial School of Stomatology Dalian Medical University

In the present study, we developed a highly sensitive and convenient biosensor consisting of gold nanoparticle(Au NP) probes and a gene chip to detect micro RNAs(mi RNAs). Specific oligonucleotides were attached to the glass surface as capture probes for the target mi RNAs, which were then detected via hybridization to the Au NP probes. The signal was amplified via the reduction of HAu Cl4 by H2O2. The use of a single Au NP probe detected 10 pmol L?1 of target mi RNA. The recovery rate for mi R-126 from fetal bovine serum was 81.5%–109.1%. The biosensor detection of mi R-126 in total RNA extracted from lung cancer tissues was consistent with the quantitative PCR(q PCR) results. The use of two Au NP probes further improved the detection sensitivity such that even 1 fmol L?1 of target mi R-125a-5p was detectable. This assay takes less than 1 h to complete and the results can be observed by the naked eye. The platform simultaneously detected lung cancer related mi R-126 and mi R-125a-5p. Therefore, this low cost, rapid, and convenient technology could be used for ultrasensitive and robust visual mi RNA detection.

关键词： microRNA 金纳米粒子视觉检测芯片检测官能化 DNA miRNA 探针杂交

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上肢康复机器人被动变刚度驱动器建模与试验

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机械工程学报 2024年第3期60卷 47-54页

作者：李守忠管昀毅马冲赵剑龙赵宏哲中国农业大学工学院北京100083 北京航空航天大学机器人研究所北京100191

康复机器人的关节往往要求良好的变刚度特性以适应人体的柔顺性。根据上肢的关节肌肉特点,对上肢康复圆周轨迹训练中不同位置的刚度需求进行分析,并在此基础上设计了一种新型被动变刚度驱动器,其通过凸轮-滚子-簧片机构产生变化的阻力矩... 详细信息

康复机器人的关节往往要求良好的变刚度特性以适应人体的柔顺性。根据上肢的关节肌肉特点,对上肢康复圆周轨迹训练中不同位置的刚度需求进行分析,并在此基础上设计了一种新型被动变刚度驱动器,其通过凸轮-滚子-簧片机构产生变化的阻力矩,从而实现上肢圆周轨迹训练过程中不同位置的刚度变化。建立了被动变刚度装置的数学模型并通过仿真和试验验证了模型的准确性和设计方案的可行性。设计的被动变刚度驱动器满足上肢康复训练过程中的刚度变化规律,结构简单且无需额外驱动与控制。

关键词：上肢康复被动变刚度簧片数学模型

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Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers

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Nano-Micro Letters 2019年第2期11卷 24-36页

作者： Lin Zhou Kun Wang Hao Sun Simin zhao Xianfeng Chen Dahong Qian Hongju Mao jianlong zhao State Key Laboratory of Transducer Technology Key Laboratory of Terahertz Solid-State TechnologyShanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050People’s Republic of China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China School of Electronic Engineering Bangor UniversityBangor LL57 1UTUK

A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors(GFETs) based on multifunctional nano-denatured bovine serum albumin(nano-dBSA) functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen(anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.

关键词： Bio-interface Multifunctional denatured BSA GFET biosensor Cancer biomarker

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基于信任理论的北京大学图书馆用户关系管理新探

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大学图书馆学报 2024年第1期42卷 11-16页

作者：陈建龙赵飞及桐北京大学图书馆北京大学信息管理系北京100871 北京大学图书馆北京100871

如何开展用户关系管理是高校图书馆现代化发展道路上必然面临的问题。北京大学图书馆将用户作为图书馆可持续发展的关键要素,自2018年以来,基于用户信任统筹推进用户关系管理,秉持真抓实干、重在参与、实在受益、贵在信任、凝心聚力的... 详细信息

如何开展用户关系管理是高校图书馆现代化发展道路上必然面临的问题。北京大学图书馆将用户作为图书馆可持续发展的关键要素,自2018年以来,基于用户信任统筹推进用户关系管理,秉持真抓实干、重在参与、实在受益、贵在信任、凝心聚力的战略方针,构建相互信赖、深度协作、长期友好的用户关系,取得明显成效。文章对基于信任理论的北京大学图书馆用户关系管理的实践探索进行初步总结。

关键词：北京大学图书馆信任用户关系关系管理

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