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Structural dynamics of the yeast Shwachman- Diamond syndrome protein （Sdol） on the ribosome and its implication in the 60S subunit maturation

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Protein & Cell 2016年第3期7卷 187-200页

作者： Chengying Ma Kaige Yan Dan Tan Ningning Li Yixiao Zhang Yi Yuan Zhifei Li Meng-Qiu Dong jianlin lei Ning Gao School of Life Sciences Tsinghua University Beijing 100084 China National Institute of Biological Sciences Beijing 102206 China Graduate Program in Chinese Academy of Medical Sciences and Pekinq Union Medical College Beiiin.q 100730 China

The human Shwachman-Diamond syndrome （SDS） is an autosomal recessive disease caused by mutations in a highly conserved ribosome assembly factor SBDS. The functional role of SBDS is to cooperate with another assembly factor, elongation factor l-like （Eft1）, to pro- mote the release of eukaryotic initiation factor 6 （elF6） from the late-stage cytoplasmic 60S precursors. In the present work, we characterized, both biochemically and structurally, the interaction between the 60S subunit and SBDS protein （Sdolp） from yeast. Our data show that Sdolp interacts tightly with the mature 60S subunit in vitro through its domain I and II, and is capable of bridging two 60S subunits to form a stable 2：2 dimer. Structural analysis indicates that Sdolp bind to the ribosomal P-site, in the proximity of uL16 and uL5, and with direct contact to H69 and H38. The dynamic nature of Sdolp on the 60S subunit, together with its strategic binding position, suggests a surveillance role of Sdolp in monitoring the conformational maturation of the ribosomal P-site. Altogether, our data support a con- formational signal-relay cascade during late-stage 60S maturation, involving uL16, Sdolp, and Efllp, which interrogates the functional P-site to control the departure of the anti-association factor elF6.

关键词： ribosome biogenesis SBDS SDS Sdol,cryo-electron microscopy (cryo-EM)

Structural insights into the assembly of the 30S ribosomal subunit in vivo： functional role of S5 and location of the 17S rRNA precursor sequence

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Protein & Cell 2014年第5期5卷 394-407页

作者： Zhixiu Yang Qiang Guo Simon Goto Yuling Chen Ningning Li Kaige Yan Yixiao Zhang Akira Muto Haiteng Deng Hyouta Himeno jianlin lei Ning Gao Ministry of Education Key Laboratory of Protein Sciences Center for Structural Biology School of Life Sciences Tsinghua University Beijing 100084 China Department of-Biochemistry and Molecular Biology Faculty of Agriculture and Life Science Hirosaki University Hirosaki 036-8561 Japan

The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA precursors, as well as modifications of selected bases. In the cell, a large number of factors are required to ensure the efficiency and fidelity of subunit production. Here we characterize the immature 30S subunits accumulated in a factor-null Escherichia coil strain （ArsgAArbfA）. The immature 30S subunits isolated with varying salt concentrations in the buffer system show interesting differences on both protein composition and structure. Specifically, inter- mediates derived under the two contrasting salt condi- tions （high and low） likely reflect two distinctive assembly stages, the relatively early and late stages of the 3＇ domain assembly, respectively. Detailed structural analysis demonstrates a mechanistic coupling between the maturation of the 5＇ end of the 17S rRNA and the assembly of the 30S head domain, and attributes a unique role of S5 in coordinating these two events. Furthermore, our structural results likely reveal thelocation of the unprocessed terminal sequences of the 17S rRNA, and suggest that the maturation events of the 17S rRNA could be employed as quality control mech- anisms on subunit production and protein translation.

关键词： RsgA RbfA ribosome assembly cryo-EM,quantitative mass spectrometry

Calculation　and　Illustration　of　Section-Projection　Diagrams　of　Quasicrystals

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Wuhan University Journal of Natural Sciences 1997年第1期2卷 42-46页

作者： lei jianlin Wang Renhui Ding Dihua Wang Zhouguang Department of Physics Wuhan University Wuhan China Institute of High Energy Physics Chinese Academy of Sciences Beijing China

On the basis of cut and projection description of quasicrystals, a method for calculating section projection diagrams of quasicrystals is developed and applied to decagonal quasicrystals. Firstly, a section projection diagram parallel to the quasiperiodic plane of perfect decagonal quasicrystal is calculated. By using the existing elastic displacement field formulae, section projection diagrams parallel to the quasiperiodic plane of a decagonal quasicrystal containing a straight dislocation along periodic direction have been calculated when only phason strain and both phason and phonon strains are introduced respectively.

关键词： quasicrystal projection method section projection diagram

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皮炎净乳膏的制备及质量控制

中国药房 2005年第23期16卷 1788-1789页

作者：雷建林李芳西安交通大学第二医院药剂科西安市710004 陕西必康药业集团有限责任公司西安市710075

目的:制备皮炎净乳膏并建立其质量控制方法.方法:采用理化反应对皮炎净乳膏进行定性鉴别;采用高效液相色谱法测定主药丙酸氯倍他索的含量.结果:理化鉴别反应呈阳性;丙酸氯倍他索检测浓度线性范围为4.06～97.49μg/ml,平均加样回收率为98... 详细信息

目的:制备皮炎净乳膏并建立其质量控制方法.方法:采用理化反应对皮炎净乳膏进行定性鉴别;采用高效液相色谱法测定主药丙酸氯倍他索的含量.结果:理化鉴别反应呈阳性;丙酸氯倍他索检测浓度线性范围为4.06～97.49μg/ml,平均加样回收率为98.67%(RSD=0.82%,n=9),在乳膏中的平均含量为0.492mg/g.结论:本处方设计及制备工艺合理,性质稳定,质量控制方法简便、准确、可行.

关键词：皮炎净乳膏丙酸氯倍他索制备质量控制

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气相色谱法测定阿奇霉素原料中的溶媒残留量

中国药房 2006年第22期17卷 1734-1736页

作者：雷建林李芳西安交通大学第二医院药剂科西安市710004 陕西必康药业集团有限责任公司西安市710075

目的建立以气相色谱法测定阿奇霉素原料中残留溶媒二氯甲烷、丙酮和乙醇含量的方法。方法色谱柱为玻璃柱,进样温度为140℃,柱温为160℃,载气为氮气,柱前压为30Psi,并以氢火焰离子化检测器进行检测。结果二氯甲烷、丙酮、乙醇检测浓度分... 详细信息

目的建立以气相色谱法测定阿奇霉素原料中残留溶媒二氯甲烷、丙酮和乙醇含量的方法。方法色谱柱为玻璃柱,进样温度为140℃,柱温为160℃,载气为氮气,柱前压为30Psi,并以氢火焰离子化检测器进行检测。结果二氯甲烷、丙酮、乙醇检测浓度分别在0·02%～0·1%(r=0·9995)、0·05%～0·25%(r=0·9994)、0·02%～0·1%(r=0·9994)范围内线性关系良好;平均回收率分别为99·1%(RSD=1·4%)、100·1%(RSD=1·1%)、99·5%(RSD=1·3%)。3批阿奇霉素原料中有机溶媒残留量均符合规定。结论本方法灵敏、准确、可靠,可用于本品有机溶媒残留量的测定。

关键词：气相色谱法阿奇霉素残留溶媒

Gene therapy for rat myelodysplastic syndrome (MDS)

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中国实验血液学杂志 1997年第3期5卷 323-324页

作者： Baozhung Feng, jianlin lei, Zc.xi Lin Institute of Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300020

During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedthe sites of the rearrangement by v-erbB PCR genediagnosis of MDS and confirmed their etiogenicsignificance by the follow-up of MDS cases. These resultsprovided 2 target sequences of ***

关键词： antisense deletion rearrangement amplification targeted curative DMBA usefulness reproduced briefly

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勐满分公司气刺微割试验总结

热带农业科学 2010年第8期30卷 8-10页

作者：雷建林云南天然橡胶股份有限公司勐满分公司云南勐腊666311

4年的气刺微割试验结果表明,气刺微割能明显提高产量和割胶劳动生产率,降低干胶含量,而死皮等副作用不明显。采用气刺微割后,必须控制增产幅度,以保持胶树的长期高产稳产。

关键词：橡胶树气刺微割:割胶速度

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非洲猪瘟病毒的生物学特性与疫苗研制的难点

生物工程学报 2020年第1期36卷 13-24页

作者：雷建林曹宏杨丽霞李茜王应强陇东学院农林科技学院

非洲猪瘟(African swine fever,ASF)是由非洲猪瘟病毒(African swine fever virus,ASFV)引起的一种猪烈性传染病,是全球养猪业的"头号杀手",强毒株引发的超急性和急性感染死率高达100%。2018年8月ASF首次传入我国,截止2019年6月6日,已... 详细信息

非洲猪瘟(African swine fever,ASF)是由非洲猪瘟病毒(African swine fever virus,ASFV)引起的一种猪烈性传染病,是全球养猪业的"头号杀手",强毒株引发的超急性和急性感染死率高达100%。2018年8月ASF首次传入我国,截止2019年6月6日,已有32个省份累计暴发137起疫情,给我国社会、经济构成巨大威胁。ASF疫苗的研制始于20世纪60年代,但均以失败而告终,其主要原因是对ASFV生物学特性缺乏深入的研究。有效控制当前ASF疫情扩散、研制安全有效的疫苗将是我国面临的巨大挑战。本文对ASFV形态与基本结构、传播途径、致病机制、基因组及编码蛋白、入侵机制、免疫逃逸等生物学特性进行了概述,并分析了当前疫苗研制面临的难点,以期为我国有效控制ASF疫情及病原研究提供参考。

关键词：非洲猪瘟非洲猪瘟病毒养猪业生物学特性疫苗研制

Preparation and applications of calcium ferrite as a functional material:A review

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 292-310页

作者： Xiuli Han Bowen Duan lei Liu Shilong Fang Weiwei Wang College of Mining Engineering North China University of Science and TechnologyTangshan 063210China Collaborative Innovation Center of Green Development and Ecological Restoration of Mineral Resources of Hebei Province Tangshan 063210China Key Laboratory of Mining Development and Security Technology of Hebei Province Tangshan 063210China

Calcium ferrite(CF)is recognized as a potential green and efficient functional material because of its advantages of magnetism,electrochemistry,catalysis,and biocompatibility in the fields of materials chemistry,environmental engineering,and ***-fore,the obtained research results need to be systematically summarized,and new perspectives on CF and its composite materials need to be *** on the presented studies of CF and its composite materials,the types and structures of the crystal are *** addition,the current application technologies and theoretical mechanisms with various properties in different fields are ***,the various preparation methods of CF and its composite materials are elaborated in *** importantly,the advantages and disadvantages of the synthesis methods of CF and its composite materials are discussed,and the existing problems and emerging challenges in practical production are ***,the key future research directions of CF and its composite materials have been prospected from the potential application technologies to provide references for its synthesis and efficient utilization.

关键词： calcium ferrite mineral functional materials preparation application perspectives