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Food Science and Human Wellness 2024年

作者： Xiaoyu Song Zhuoting Luo Mengting li jianke li Chen Hou College of Food Engineering and Nutritional Science Shaanxi Normal University University Key Laboratory of Food Processing Byproducts for Advanced Development and High Value Utilization Shaanxi Normal University

High-fat diet (HFD) promotes the imbalance of gut microbiota， contributing to the development of metabolic disorders. Pomegranate juice (PJ) and inulin， as dietary polyphenol and dietary fiber respectively， have been reported to ameliorate diet-induced gut microbiota dysbiosis and metabolic disorders. However， the combined effect of PJ and inulin on HFD-induced metabolic disorders has not been elucidated. In current study， we found that the combination of PJ and inulin prevent HFD-induced obesity， dyslipidemia， and gut microbiota dysbiosis. Moreover， the fecal levels of short-chain fatty acids (SCFAs) produced by inulin were increased after inulin combined with PJ. The levels of pomegranate polyphenol metabolites produced by PJ were also increased after PJ combined with inulin， especially pharmacokinetic analysis showed that the presence of inulin enhance the absorption level of urolithin A. Fecal metabolomic analysis found that PJ combined with inulin alter the metabolic status compared with PJ. It was also showed that the levels of SCFAs and pomegranate polyphenol metabolites among groups were correlated with gut microbiota and metabolic disorders indicators. Our results suggested that PJ combined with inulin prevent HFD-induced metabolic disorders， which may be attributed to the promotion of mutual metabolic transformation and absorption of PJ and inulin by gut microbiota.

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Pomegranate peel polyphenols improves diet-induced obesity and promotes adipose browning development through gut microbiota in mice

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Food Science and Human Wellness 2024年

作者： Zhuoting Luo Xiaoyu Song Mengting li jianke li Chen Hou University Key Laboratory of Food Processing Byproducts for Advanced Development and High Value Utilization Shaanxi Normal University College of Food Engineering and Nutritional Science Shaanxi Normal University

Gut microbiota regulate the activation of adipose browning， which promote energy dissipation and combat diet-induced obesity. Pomegranate peel polyphenols (PPPs) have been shown to reduce obesity， regulate lipid metabolism in adipose tissue， and modulate the composition of gut microbiota in animal fed high-fat diet (HFD). However， the role of gut microbiota in the improvement of obesity by PPPs has not been elucidated. In current study， we applied antibiotics to inhibit gut microbiota in mice fed HFD and treated with PPPs. The results showed that the inhibition of gut microbiota impair the effect of PPPs on reducing obesity and promoting adipose browning， and change the fecal metabolomic profiles in respond to PPPs. Moreover， the inhibition of gut microbiota supressed the promotive effects of PPPs on the levels of Akkermansia and microbiota-related metabolites， such as urolithin A， short-chain fatty acids (SCFAs)， and bile acids (BAs)， which were associated with activating adipose browning. Therefore， our results suggested that the presence of gut microbiota is essential for PPPs to ameliorate HFD-induced obesity. The related bacteria or metabolites generated by the interaction between PPPs and microbiota promote adipose browning and facilitate the beneficial effects of PPPs.

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Beneficial effects of lacquer seed oil on bromocriptine-induced postpartum hypogalactia in rats

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Food Science and Human Wellness 2024年

作者： Mengting li Xiaoyu Song lin Shi Jie li Mayira·Ahat Yaojie liu Chen Hou jianke li University Key Laboratory of Food Processing Byproducts for Advanced Development and High Value Utilization Shaanxi Normal University College of Food Engineering and Nutritional Science Shaanxi Normal University

The high incidence of postpartum hypogalactia hinders the healthy development of postpartum women and the next generation. Lacquer seed oil (LSO)， the oil extracted from the seeds of the lacquer tree， has been traditionally used as a dietary supplement for promoting postpartum lactation and recovery in some districts of China. However， its physiological effects have not been verified， and the mechanism and active components of LSO have not been analyzed. Thus， we applied LSO to bromocriptine-induced postpartum hypogalactia rats. The results showed that LSO supplement effectively improves bromocriptine-induced postpartum hypogalactia. LSO also increased prolactin levels reduced by bromocriptine， promoted JAK2/STAT5 and PI3K/AKT pathways and several gene expression levels of milk synthesis in mammary gland. Moreover， metabolomic and network pharmacological analysis further revealed that JAK2/STAT5， PI3K/AKT， and estrogen signaling pathway are the potential main regulatory sites for the beneficial effects of LSO on postpartum hypogalactia， and that quercetin， kaempferol， arachidonic acid， epicatechin， and β-sitosterol are the top 5 main active ingredients of LSO. Our results suggested that LSO has great potential in the application of the improvement of postpartum hypogalactia.

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Reinforcement of Clay Soils through Fracture Grouting

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Fluid Dynamics & Materials Processing 2022年第6期18卷 1649-1665页

作者： Shaozhen Cheng Tielin Chen Zizhou Xue Kang Zhu jianke li School of Civil Engineering Beijing Jiaotong UniversityBeijing100044China Central Research Institute of Building and Construction Co. Ltd.MCC GroupBeijing100088China Beijing Geotechnical Anchorage Engineering Center China Jingye Engineering Co.Ltd.Beijing100088China

Fracture grouting is widely used for building foundation reinforcement,however the underpinning mechanisms are still not clear.Using numerical results about a single-hole fracture grouting process as a basis,a model composed of soil and grouting veins has been created to analyze the reinforcement mechanism.The influence weights of the grouting vein skeleton and compaction effect have been studied,thereby obtaining relevant information on the compressive modulus of the considered composite soil.The research results show that the compaction effect plays a leading role in the soil fracture grouting reinforcement.The grouting pressure,the hardened grouting vein modulus,and the shape of the grouting veins all influence the compressive modulus of the composite soil.

关键词： Building foundation fracture grouting composite soil reinforcement mechanism reinforcement effect

Pomegranate peel polyphenols alleviate insulin resistance through the promotion of insulin signaling pathway in skeletal muscle of metabolic syndrome rats

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Food Science and Human Wellness 2022年第4期11卷 1076-1085页

作者： Xitong Zhang lin Du Weimin Zhang Mi Yang li Chen Chen Hou jianke li College of Food Engineering and Nutritional Science Shaanxi Normal UniversityXi’an 710119China University Key Laboratory of Food Processing Byproducts for Advanced Development and High Value Utilization Xi’an 710119China

Insulin resistance(IR) has been considered to be an important causative factor of metabolic syndrome(Met S). The present study investigated whether pomegranate peel polyphenols(PPPs) could prevent the development of Met S by improving IR in rats. Male Sprague-Dawley(SD) rats were fed high fat diet(HFD) to induce Met S and supplemented with different dosages of PPPs for 12 weeks. The results showed that HFD-induced insulin resistant rats had disordered metabolism of blood glucose, blood lipid, and terrible muscle fiber morphology when compared with normal diet-fed rats, but PPPs treatment at a dosage of 300 mg/kg·day significantly reversed these negative effects. Moreover, in skeletal muscle tissue of insulin resistant rats, PPPs treatments significantly increased the protein expressions of insulin receptor(Ins R) and phosphorylated insulin receptor substrate 1(IRS-1), stimulated peroxisome proliferator activated receptor gamma(PPARγ) and phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT/PKB) signaling pathway, and aggrandized the protein levels of phosphorylated glycogen synthase kinase-3β(GSK-3β) and glucose transporter 4(GLUT4). Our results suggest that PPPs possess of the beneficial effects on alleviating IR by enhancing insulin sensitivity and regulating glucose metabolism.

关键词： Metabolic syndrome Insulin resistance Insulin signaling pathway PPARγ Pomegranate peel polyphenols

Expression of synaptosomal-associated protein-25 in the rat brain after subarachnoid hemorrhage

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Neural Regeneration Research 2013年第29期8卷 2693-2702页

作者： Gang Chen Tong Hu Qi li jianke li Yang Jia Zhong Wang Department of Neurosurgery the First Affiliated Hospital of Soochow University Department of Neurosurgery Yixing People's Hospital

Synaptosomal-associated protein-25 is an important factor for synaptic functions and cognition. In this study, subarachnoid hemorrhage models with spatial learning disorder were established through a blood injection into the chiasmatic cistern. Immunohistochemical staining and western blot analysis results showed that synaptosomal-associated protein-25 expression in the temporal lobe, hippocampus, and cerebellum significantly lower at days 1 and 3 following subarachnoid hemorrhage. Our findings indicate that synaptosomal-associated protein-25 expression was down-regulated in the rat brain after subarachnoid hemorrhage.

关键词： neural regeneration brain injury synaptosomal-associated protein-25 subarachnoid hemorrhage cognition cortex hippocampus cerebellum grants-supported paper neuroregeneration

神经酸对H_(2)O_(2)诱导PC12细胞氧化损伤的保护作用

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食品科学 2024年第9期45卷 116-123页

作者：玛依乐·艾海提刘垚杰李建科陕西师范大学食品工程与营养科学学院陕西西安710119

以PC12细胞作为研究对象、H_(2)O_(2)为诱导剂,用200μmol/L H_(2)O_(2)处理PC12细胞24 h,建立细胞氧化损伤模型。通过检测超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性以及乳酸脱氢酶(lactate dehydrogenase,LDH)和丙二醛(malondialdehyde,MDA)水平确定氧化应激程度,并对细胞凋亡和细胞内活性氧(reactive oxygen species,ROS)水平进行评估。采用蛋白免疫印迹和实时聚合酶链式反应检测B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)、Bcl-2相关X蛋白质(Bcl-2-associated X protein,Bax)、半胱氨酸蛋白酶-3(Caspase-3)、核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)、kelch样ECH关联蛋白1(kelch-like ECH-associated protein 1,Keap1)和血红素加氧酶-1(heme oxygenase 1,HO-1)及其基因的表达水平。结果表明,使用200μmol/L H_(2)O_(2)处理PC12细胞24 h后,其存活率为60.12%;细胞毒性实验表明,神经酸能够显著降低H_(2)O_(2)诱导损伤细胞中LDH和MDA的含量,抑制ROS的过度产生,并增强SOD和GSH-Px活性,显著上调Bcl-2、Nrf2和HO-1的表达水平,下调Caspase-3、Bax和Keap1的表达水平。综上所述,神经酸对H_(2)O_(2)诱导的PC12细胞氧化损伤具有保护作用,且与激活Nrf2/HO-1信号通路有关。

关键词：神经酸氧化损伤 PC12细胞信号通路

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华支睾吸虫体内神经递质5-羟色胺的分布

中国寄生虫学与寄生虫病杂志 2024年第1期42卷 78-82,90页

作者：刘柳张静李健科张浩张凤玉齐齐哈尔医学院医学技术学院黑龙江齐齐哈尔161000

目的了解华支睾吸虫体内神经递质5--羟色胺(5--HT)的分布规律。方法从感染麦穗鱼中消化分离华支睾吸虫囊蚴,以灌胃方式感染90只昆明小鼠(40~50个囊蚴/鼠),分别于感染后第10、20、30天安乐死处死小鼠,从肝门静脉收集虫体。醋酸洋红染色... 详细信息

目的了解华支睾吸虫体内神经递质5--羟色胺(5--HT)的分布规律。方法从感染麦穗鱼中消化分离华支睾吸虫囊蚴,以灌胃方式感染90只昆明小鼠(40~50个囊蚴/鼠),分别于感染后第10、20、30天安乐死处死小鼠,从肝门静脉收集虫体。醋酸洋红染色后光学显微镜下观察不同发育阶段虫体内各器官发育情况;同时进行5--HT免疫荧光染色,在激光扫描共聚焦荧光显微镜下观察虫体内5--HT的分布情况。结果醋酸洋红染色后光学显微镜下观察可见,感染后华支睾吸虫d10幼虫中,消化、排泄器官基本发育成熟,生殖器官未完全发育成熟,子宫内可见散在分布的虫卵;d20幼虫中,生殖器官发育成熟,子宫中虫卵数量增多,睾丸分支增多;d30成虫中,消化、排泄、生殖系统均发育成熟,子宫内虫卵较d20幼虫排列更紧密,睾丸增大且分支明显。5--HT免疫荧光染色后激光扫描共聚焦荧光显微镜下可见,d10幼虫的中枢经系统节、神经联合、口吸盘处荧光染色较强,在虫体内部器官中呈点状分布;d20幼虫的口吸盘附近神经联合处观察到少量神经细胞,除中枢神经系统外,虫体的排泄囊及排泄孔、生殖器官中出现5--HT荧光染色;d30虫体的消化、排泄、生殖器官中均出现5--HT荧光染色,睾丸的荧光染色较肠支、口腹吸盘等部位更强,虫体中存在较多神经细胞。结论5--HT广泛存在于华支睾吸虫虫体内,主要分布于肌肉组织丰富的器官及内脏。5--HT在同一发育阶段虫体内不同器官中的分布存在差异,在相同器官的不同发育阶段也存在差异。

关键词：华支睾吸虫发育 5-羟色胺

顶空-气相色谱法测定橄榄油中6种卤化溶剂残留

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色谱 2024年第4期42卷 387-392页

作者：雷春妮王波顾强张欢张晓美李建科兰州海关技术中心甘肃兰州730010 张家港海关综合技术中心江苏张家港215600 陇南市祥宇油橄榄开发有限责任公司甘肃陇南746000

卤化溶剂残留量是评价橄榄油质量的重要指标之一。国家橄榄油质量标准GB/T 23347-2021中规定了橄榄油中每种卤化溶剂残留量≤0.1 mg/kg、卤化溶剂残留量总和≤0.2 mg/kg,其检验方法是国际油橄榄理事会发布的COI/T.20/Doc.No 8-1990标准... 详细信息

卤化溶剂残留量是评价橄榄油质量的重要指标之一。国家橄榄油质量标准GB/T 23347-2021中规定了橄榄油中每种卤化溶剂残留量≤0.1 mg/kg、卤化溶剂残留量总和≤0.2 mg/kg,其检验方法是国际油橄榄理事会发布的COI/T.20/Doc.No 8-1990标准方法,该方法操作繁琐、重复性差、自动化程度低,不适于大批量橄榄油样品中卤化溶剂残留量的测定,而目前国家尚未制定橄榄油中卤化溶剂残留量测定的标准方法。该文建立了橄榄油中氯仿、四氯化碳、1,1,1-三氯乙烷、二溴氯甲烷、四氯乙烯和溴仿6种卤化溶剂残留量的顶空-气相色谱法。将橄榄油试样摇匀后,称取2.00 g(精确至0.01 g)于顶空瓶中,立即封盖待顶空-气相色谱仪分析,采用空白初榨橄榄油配制标准工作液,外标法定量。考察了顶空进样器的进样时间、平衡温度、平衡时间对6种卤化溶剂残留量检测的影响,在进样时间3 s、平衡温度90℃、平衡时间30 min时6种卤化溶剂的分析效果较好。结果表明:6种卤化溶剂在0.002~0.200 mg/kg范围内线性关系良好,相关系数≥0.9991,检出限为0.0003~0.0006 mg/kg,定量限为0.001~0.002 mg/kg,不同加标水平下的平均回收率为85.53%~115.93%,相对标准偏差(n=6)为1.11%~8.48%。该方法的6种卤化溶剂定量限显著低于COI/T.20/Doc.No 8-1990标准方法的定量限(0.02 mg/kg),且操作时间短,精密度高,准确性好,自动化程度高,适合大批量橄榄油样品中6种卤化溶剂残留量的测定分析。

关键词：顶空-气相色谱法卤化溶剂残留橄榄油

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小剂量巴瑞替尼成功治疗匐行性斑秃1例

中国皮肤性病学杂志 2024年第6期38卷 652-655页

作者：李筱娴王秀环李建可刘鑫洲刘国艳山东第一医科大学附属皮肤病医院(山东省皮肤病医院) 山东省皮肤病性病防治研究所山东济南250022

患者女,32岁,头部反复脱发斑15年伴加重4年。皮肤科情况:前额部发际线明显后移,头顶部毛发稀疏,颞侧及后枕部宽带状脱发斑,边缘锯齿状,拉发试验阳性,脱发严重度工具(SALT)评分48.6分,眉毛睫毛未见异常。毛发镜示:多发黄点征、黑点征及断... 详细信息

患者女,32岁,头部反复脱发斑15年伴加重4年。皮肤科情况:前额部发际线明显后移,头顶部毛发稀疏,颞侧及后枕部宽带状脱发斑,边缘锯齿状,拉发试验阳性,脱发严重度工具(SALT)评分48.6分,眉毛睫毛未见异常。毛发镜示:多发黄点征、黑点征及断发,散在锥形发及少量的直立新生发。诊断:匐行性斑秃。治疗:给予巴瑞替尼2 mg/d,治疗4周后脱发量减少,但新生发不明显,拉发试验转为阴性。治疗12周后复诊可见脱发斑明显减少,SALT评分9.2分。持续治疗48周,患者SALT降至0分。患者目前随访8个月仍未见复发。

关键词：匐行性斑秃巴瑞替尼小剂量