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Antagonistic MADS-box transcription factors SEEDSTICK and SEPALLATA3 form a transcriptional regulatory network that regulates seed oil accumulation

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Journal of Integrative Plant Biology 2024年第1期66卷 121-142页

作者： Shuangcheng He Yuanchang Min Zijin Liu Fang Zhi rong Ma Ankang Ge Shixiang Wang Yu Zhao Danshuai Peng Da Zhang Minshan Jin Bo Song jianjun Wang Yuan Guo Mingxun Chen State Key Laboratory for Crop Stress Resistance and High‐Efficiency Production National Yangling Agricultural Biotechnology&Breeding CenterShaanxi Key Laboratory of Crop HeterosisCollege of AgronomyNorthwest A&F UniversityYangling 712100China State Key Laboratory for Crop Stress Resistance and High‐Efficiency Production Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYangling 712100China

Transcriptional regulation is essential for balancing multiple metabolic pathways that influence oil accumulation in *** far,the transcriptional regulatory mechanisms that govern seed oil accumulation remain largely ***,we identified the transcriptional regulatory network composed of MADS-box transcription factors SEEDSTICK(STK)and SEPALLATA3(SEP3),which bridges several key genes to regulate oil accumulation in *** found that STK,highly expressed in the developing embryo,positively regulates seed oil accumulation in Arabidopsis(Arabidopsis thaliana).Furthermore,we discovered that SEP3 physically interacts with STK in vivo and in *** oil content is increased by the SEP3 mutation,while it is decreased by SEP3 *** chromatin immunoprecipitation,electrophoretic mobility shift assay,and transient dual-luciferase reporter assays showed that STK positively regulates seed oil accumulation by directly repressing the expression of MYB5,SEP3,and SEED FATTY ACID REDUCER 4(SFAR4).Moreover,genetic and molecular analyses demonstrated that STK and SEP3 antagonistically regulate seed oil production and that SEP3 weakens the binding ability of STK to MYB5,SEP3,and ***,we demonstrated that TRANSPARENT TESTA 8(TT8)and ACYL-ACYL CARRIER PROTEIN DESATURASE 3(AAD3)are direct targets of MYB5 during seed oil accumulation in ***,our findings provide the transcriptional regulatory network antagonistically orchestrated by STK and SEP3,which fine tunes oil accumulation in seeds.

关键词： seed oil accumulation SEP3 STK transcription factor transcriptional regulation

A new catalyst for urea oxidation: NiCo2S4 nanowires modified 3D carbon sponge

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Journal of Energy Chemistry 2020年第11期29卷 195-205页

作者： Biaopeng Li Congying Song jianjun rong Jing Zhao Hong-En Wang Ping Yang Ke Ye Kui Cheng Kai Zhu Jun Yan Dianxue Cao Guiling Wang Key Laboratory of Superlight Materials and Surface Technology of Ministry of Education College of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001HeilongjiangChina State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070HubeiChina

Urea oxidation is a significant reaction for utilizing urea-rich wastewater or human urine as sustainable power sources which can ease the water eutrophication while generate electricity. A direct urea-hydrogen peroxide fuel cell is a new kind of fuel cell employing urea as fuel and hydrogen peroxide as oxidant which possesses a larger cell voltage. Herein, this work tries to promote the kinetics process of urea oxidation by preparing low-cost and high-efficient NiCo2S4 nanowires modified carbon sponge electrode. The carbon sponge used in this work with a similar three-dimensional multi-channel structure to Ni foam, is prepared by carbonizing recycled polyurethane sponge which is also a process of recycling waste. The performance of the prepared catalyst in an alkaline solution is investigated in a three-electrode *** the introduction of Co element to the catalyst, a reduced initial urea oxidation potential and a high performance are obtained. Furthermore, a direct urea-hydrogen peroxide fuel cell is assembled using the NiCo2S4 nanowires modified carbon sponge anode. Results indicate that the prepared catalyst provides a chance to solve the current problems that hinder the development of urea electrooxidation(high initial urea oxidation potential, low performance, and high electrode costs).

关键词： Carbon sponge Direct urea fuel cell Electrocatalyst NiCo2S4 nanowires Urea oxidation

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科研机构整体绩效评价指标体系优化研究

图书情报工作 2024年第11期68卷 72-81页

作者：何峻于建军荣晓慧中国科学院大学经济与管理学院管理科学与工程系北京100871 中国科学院计算机网络信息中心北京100871

[目的/意义]科研机构绩效评价能够对科研机构的科研活动及其产出和影响的价值进行有效评价,建立科学合理的绩效评价指标体系是绩效评价的核心内容。[方法/过程]设计一套系统性的分领域、分区域的绩效评价指标体系,为支撑科研评价改革提... 详细信息

[目的/意义]科研机构绩效评价能够对科研机构的科研活动及其产出和影响的价值进行有效评价,建立科学合理的绩效评价指标体系是绩效评价的核心内容。[方法/过程]设计一套系统性的分领域、分区域的绩效评价指标体系,为支撑科研评价改革提供绩效评价指标构建方法。针对科研机构绩效评价的特点,构建基于随机森林算法的科研机构绩效评价指标体系优化模型。通过分析现有指标体系中指标的贡献值,筛选出绩效评价共性关键指标,同时在优化指标体系中,针对不同学科领域和区域,分别分析其指标体系中指标的重要性,优化共性指标和个性指标的结构权重比例,建立一套系统性的分领域、区域的科研机构绩效评价指标体系。[结果/结论]该指标体系在最大化地满足科研机构评价目标设定的同时,又能针对科研机构学科领域多、区域分布广的特点,对指标进行动态、适度地调整。

关键词：绩效评价指标体系优化随机森林算法

Expert Recommendation in Community Question Answering via Heterogeneous Content Network Embedding

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Computers, Materials & Continua 2023年第4期75卷 1687-1709页

作者： Hong Li jianjun Li Guohui Li rong Gao Lingyu Yan School of Computer Science and Technology Huazhong University of Science and TechnologyWuhan 430074China College of Computer Science and Technology Hubei University of TechnologyWuhan 430068China

ExpertRecommendation(ER)aims to identify domain experts with high expertise and willingness to provide answers to questions in Community Question Answering(CQA)web *** to model questions and users in the heterogeneous content network is critical to this *** traditional methods focus on modeling questions and users based on the textual content left in the community while ignoring the structural properties of heterogeneous CQA networks and always suffering from textual data sparsity *** approaches take advantage of structural proximities between nodes and attempt to fuse the textual content of nodes for ***,they often fail to distinguish the nodes’personalized preferences and only consider the textual content of a part of the nodes in network embedding learning,while ignoring the semantic relevance of *** this paper,we propose a novel framework that jointly considers the structural proximity relations and textual semantic relevance to model users and questions more ***,we learn topology-based embeddings through a hierarchical attentive network learning strategy,in which the proximity information and the personalized preference of nodes are encoded and ***,we utilize the node’s textual content and the text correlation between adjacent nodes to build the content-based embedding through a meta-context-aware skip-gram *** addition,the user’s relative answer quality is incorporated to promote the ranking *** results show that our proposed framework consistently and significantly outperforms the state-of-the-art baselines on three real-world datasets by taking the deep semantic understanding and structural feature learning *** performance of the proposed work is analyzed in terms of MRR,P@K,and MAP and is proven to be more advanced than the existing methodologies.

关键词： Heterogeneous network learning expert recommendation semantic representation community question answering

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甜瓜白粉病病原菌鉴定及抗病种质资源筛选

中国蔬菜 2024年第3期 67-74页

作者：陈建军王锋陈积豪梁其干符小发范蓉张浩王敏海南省农业科学院三亚研究院海南省实验动物研究中心海南三亚572025 新疆农业科学院海南三亚农作物育种试验中心海南三亚572000 新疆农业科学院哈密瓜研究中心新疆乌鲁木齐830091

三亚是海南甜瓜的重要产区,也是甜瓜白粉病的高发区。利用镜检法、分子生物学方法以及国际通用的13个标准鉴别寄主对三亚甜瓜试验基地自然发病的白粉病病原菌进行生理小种鉴定,并从自主选育的第4代甜瓜种质资源中挑选30个材料进行白粉... 详细信息

三亚是海南甜瓜的重要产区,也是甜瓜白粉病的高发区。利用镜检法、分子生物学方法以及国际通用的13个标准鉴别寄主对三亚甜瓜试验基地自然发病的白粉病病原菌进行生理小种鉴定,并从自主选育的第4代甜瓜种质资源中挑选30个材料进行白粉病苗期抗病性鉴定和筛选。结果表明,通过病原菌孢子的形态特征分析、ITS序列比对以及13个标准寄主对白粉病病原菌接种的感抗反应,综合判定该病原菌为瓜单囊壳白粉菌(Podosphaera xanthii)的Race 2France生理小种。另外,从30份厚皮甜瓜材料中筛选出对白粉病免疫的材料2份(22-7-2、22-16-1),对白粉病高抗的材料3份(06-4-1、14-8-1、23-8-1);2份免疫材料均为黄皮、椭圆、网纹类型,3份高抗材料均为网纹类型。

关键词：甜瓜白粉病生理小种抗性鉴定

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石油压裂的电力驱动与储能系统的应用

变频器世界 2024年第4期27卷 70-73页

作者：黄建军魏继云荣凯曹广芹新风光电子科技股份有限公司山东272500

压裂技术是针对石油、天然气等储存在地下的能源而开发出来的提高产量的一种方法。而由变频驱动的电驱压裂是页岩能源开采设备的必然发展趋势,具备排量精准控制、功率密度大等优势。在部分现场不具备电网的条件下,燃机加储能在页岩油气... 详细信息

压裂技术是针对石油、天然气等储存在地下的能源而开发出来的提高产量的一种方法。而由变频驱动的电驱压裂是页岩能源开采设备的必然发展趋势,具备排量精准控制、功率密度大等优势。在部分现场不具备电网的条件下,燃机加储能在页岩油气的压裂中也应运而生。由变频驱动的电驱压裂及提供能源储备的储能为石油行业的降本增效、节能减排发挥重要作用。

关键词：电驱压裂页岩油气变频器储能系统

金刚丸联合骨化三醇治疗肾阳亏虚型老年骨质疏松症的临床研究

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湖南中医药大学学报 2024年第5期44卷 822-827页

作者：匡昱林欧梁匡建军戎宽孙绍裘湖南中医药大学湖南长沙410208 湖南中医药大学第二附属医院湖南长沙410005 湖南省中医药研究院湖南长沙410008

目的探讨金刚丸联合骨化三醇治疗肾阳亏虚型老年骨质疏松症的临床疗效及安全性。方法选取湖南省中医药研究院附属医院门诊接受治疗的72名肾阳亏虚型骨质疏松的老年患者,采用分层随机化方法随机分为对照组(骨化三醇治疗)和治疗组(金刚丸... 详细信息

目的探讨金刚丸联合骨化三醇治疗肾阳亏虚型老年骨质疏松症的临床疗效及安全性。方法选取湖南省中医药研究院附属医院门诊接受治疗的72名肾阳亏虚型骨质疏松的老年患者,采用分层随机化方法随机分为对照组(骨化三醇治疗)和治疗组(金刚丸联合骨化三醇治疗),每组36例。治疗持续8周。比较两组患者的临床疗效、视觉模拟评分法(visual analogue scale,VAS)评分、骨密度、骨代谢指标、成骨相关基因水平、炎症因子水平、生活质量、中医证候积分和安全性。结果治疗组总有效率为91.7%,高于对照组的69.4%(P<0.05);与对照组比较,治疗组VAS评分显著降低(P<0.05),全髋、腰椎及股骨颈骨密度均增加(P<0.05),治疗组骨形态发生蛋白2(bone morphogenetic protein 2,BMP2)RNA表达水平、骨碱性磷酸酶(bone alkaline phosphatase,BALP)、骨钙素(osteocalcin,BGLAP)水平、健康状况调查问卷(36-item short-form,SF-36)评分升高(P<0.05),抗酒石酸酸性磷酸酶5b(tartrateresistant acid phosphatase 5b,TRACP-5b)水平、miR-133aRNA表达水平、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平、白细胞介素-6(interleukin-6,IL-6)水平及中医临床症状评分降低(P<0.05)。结论金刚丸联合骨化三醇可能通过上调BALP、BGLAP、BMP2,下调TRACP-5b、miR-133a、TNF-α及IL-6,改善患者骨密度、骨代谢、炎症状态及生活质量,能有效显著提高“肾阳亏虚证”肾阳亏虚型老年骨质疏松症患者的治疗效果。

关键词：老年骨质疏松症肾阳亏虚证金刚丸骨化三醇临床疗效视觉模拟评分法

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脊柱-骨盆矢状位参数与腰椎退行性疾病术后持续性下腰痛的关系

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实用骨科杂志 2024年第2期30卷 115-119,152页

作者：袁建军李广张权任志帅刘岩田融天津市人民医院脊柱脊髓病诊疗中心天津300121

目的分析脊柱-骨盆矢状位序列参数变化与腰椎退行性疾病(degenerative lumbar disease,DLD)患者术后持续性下腰痛的关系。方法选取天津市人民医院2019年6月至2022年6月行单节段腰椎融合内固定术治疗的86例DLD患者,根据术后是否发生持... 详细信息

目的分析脊柱-骨盆矢状位序列参数变化与腰椎退行性疾病(degenerative lumbar disease,DLD)患者术后持续性下腰痛的关系。方法选取天津市人民医院2019年6月至2022年6月行单节段腰椎融合内固定术治疗的86例DLD患者,根据术后是否发生持续性下腰痛分为腰痛组和非腰痛组。腰痛组34例,男10例,女24例;年龄32~77岁,平均(59.36±8.65)岁。非腰痛组52例,男21例,女31例;年龄30~78岁,平均(56.81±8.03)岁。比较两组患者一般资料、手术前后疼痛视觉模拟评分(visual analogue score,VAS)、日本骨科协会(Japanese orthopaedic association,JOA)腰椎功能评分及脊柱-骨盆矢状位序列参数[腰椎前凸角(lumbar lordosis,LL)、骨盆入射角(pelvic incidence,PI)、骨盆倾斜角(pelvic tilt,PT)、骶骨倾斜角(sacral slope,SS)]。分析术后持续性下腰痛发生的影响因素,分析手术前后脊柱-骨盆矢状位序列参数变化值与VAS、JOA评分变化值的相关性,分析手术前后脊柱-骨盆矢状位序列参数变化值及相关影响因素预测术后发生持续性下腰痛的价值。结果腰痛组骨质疏松、术中骨性终板损伤发生率(47.06%、23.53%)高于非腰痛组(15.38%、1.92%);腰痛组术后3个月VAS、PT高于非腰痛组,JOA评分、LL、SS低于非腰痛组,ΔVAS、ΔJOA、ΔLL、ΔPI、ΔPT、ΔSS小于非腰痛组(P0.05);骨质疏松、术中骨性终板损伤、ΔLL、ΔPT、ΔSS均为DLD患者术后发生持续性下腰痛的影响因素(P0(P<0.05)。结论脊柱-骨盆矢状位序列参数变化与DLD患者腰椎术后持续性下腰痛的发生有关,且在预测术后发生持续性下腰痛方面具有良好应用价值。

关键词：腰椎退行性疾病持续性下腰痛脊柱-骨盆矢状位序列参数相关性分析预测

Role of ammonium sulfate in sulfurization flotation of azurite:Inhibiting the formation of copper sulfide colloid and its mechanism

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International Journal of Mining Science and Technology 2022年第3期32卷 575-584页

作者： Jinpeng Cai Chao Su Yinyu Ma Xingcai Yu rong Peng Jiangli Li Xiaolin Zhang jianjun Fang Peilun Shen Dianwen Liu State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Yunnan Key Laboratory of Green Separation and Enrichment of Strategic Metal ResourcesFaculty of Land Resources EngineeringKunming University of Science and TechnologyKunming 650093China Shandong Gold Group Co. LtdJinan 250014China Yunnan Posphate Group Co. LtdKunming 650600China

In this study,the role of(NH_(4))_(2)SO_(4)during the sulfurization of azurite and its response to flotation were *** flotation results showed that adding(NH_(4))_(2)SO_(4)prior to sulfurization decreased the formation of colloid in flotation pulp,and the floatability of the suppressed azurite caused by excess sodium sulfide was *** adding(NH_(4))_(2)SO_(4)prior to sulfurization,the formation of Cu(NH_(3))_(n) ^(2+)intermediate products changed the path of the sulfurization reaction,which slowed the direct impact of HSon the azurite *** nucleation rate was reduced,and the growth of copper sulfide crystal was ***(syn,CuS)with larger crystal grains was formed on the azurite surface,thereby enhancing the mechanical stability of copper sulfide products onto the mineral ***,the generated copper sulfide colloid significantly reduced,ultimately promoting the effective adsorption of xanthate on the azurite surface.

关键词： Azurite Sulfurization flotation Ammonium sulfate Colloid Inhibition Crystal growth