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Identification of UGT85A glycosyltransferases associated with volatile conjugation in grapevine(Vitis vinifera × Vitis labrusca)

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Horticultural Plant Journal 2023年第6期9卷 1095-1107页

作者： Xiangmei Cao Dan Jiang Hanqing Wang Boping Wu jianhui cheng Bo Zhang Laboratory of Fruit Quality Biology/Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology Zhejiang UniversityHangzhouZhejiang 310058China Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable Ministry of Agriculture and Rural AffairsCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhouZhejiang 310021China

Glycosylated volatiles are considered as hidden aroma precursors that affect fruit flavor and are involved in plant defense response. Several uridine-diphosphate(UDP)-glycosyltransferase(UGT) members related to the glycosylation of volatiles have been identified from grapevine berries. Although grapevine leaves produced much higher levels of glycosylated volatiles than berries, UGTs responsible for the production of glycosylated volatiles in leaves have not yet been identified. Here, eight novel UGT genes were cloned, among these four members that belong to UGT85A family(named UGT85A24, UGT85A25, UGT85A26, and UGT85A27) were localized in the cytoplasm and their recombinant proteins showed activity toward volatiles detected in grapevine, with geraniol as the preferred substrate in vitro. Two UGT genes with 98.1% homology differ in R/S-linalool selection. The UGT85A26-catalyzed substrate showed enrichment in S-linalool, whereas UGT85A27 showed a strong preference for R-linalool. UGT85A24 and UGT85A25 were expressed mainly in berries, and UGT85A26 and UGT85A27 were expressed predominantly in grapevine mature leaves and correlated with the highest content of glycosylated volatiles in the mature leaves. Methyl jasmonate(MeJA) induced the expression of UGT85A26 and UGT85A27 as well as the production of linalyl-β-glucoside, citronellyl-β-glucoside and phenethyl-β-glucoside. Transient overexpression of UGT85A26 and UGT85A27 in tobacco leaves led to the accumulation of linalyl-β-glucoside and an increase in citronellyl-β-glucoside in vivo. These results indicate that the expression of UGT85A26 and UGT85A27 is a major regulator affecting the content of glycosylated volatiles in grapevine.

关键词： UDP-glycosyltransferase Grape Volatiles Defense MeJA

The Harbin Love Knot of a Jewish Family

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中国周刊 2002年第11期 41-42页

作者： Liu jianhui Proofread: cheng Neisen

One of our writers wrote the article The Jewish people in Harbin. The Press and Information officers of the Israeli Embassy gave high praise to the article and suggested that I interview the Minister-Counselor of Scientific & Agricultural Affairs, Mr. Amram Olmert. Mr. Olmert’s grandfather, parents, and some relatives lived in Harbin for many years. His grandfather was buried in Harbin. Mr. Olmert talked a lot about those years and those people during the interview.

关键词： The Harbin Love Knot of a Jewish Family

Non-Darcy flow in oil accumulation (oil displacing water) and relative permeability and oil saturation characteristics of low-permeability sandstones

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Petroleum Science 2010年第1期7卷 20-30页

作者： Zeng jianhui cheng Shiwei Kong Xu Guo Kai Wang Hongyu State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum Beijing 102249 China School of Natural Resources and Information Technology China University of Petroleum Beijing 102249 China CNPC Greatwall Drilling Company Beijing 100101 China

Hydrocarbon resources in low-permeability sandstones are very abundant and are extensively distributed. Low-permeability reservoirs show several unique characteristics, including lack of a definite trap boundary or caprock, limited buoyancy effect, complex oil-gas-water distribution, without obvious oil-gas-water interfaces, and relatively low oil （gas） saturation. Based on the simulation experiments of oil accumulation in low-permeability sandstone （oil displacing water）, we study the migration and accumulation characteristics of non-Darcy oil flow, and discuss the values and influencing factors of relative permeability which is a key parameter characterizing oil migration and accumulation in low-permeability sandstone. The results indicate that： 1） Oil migration （oil displacing water） in low- permeability sandstone shows non-Darcy percolation characteristics, and there is a threshold pressure gradient during oil migration and accumulation, which has a good negative correlation with permeability and apparent fluidity; 2） With decrease of permeability and apparent fluidity and increase of fluid viscosity, the percolation curve is closer to the pressure gradient axis and the threshold pressure gradient increases. When the apparent fluidity is more than 1.0, the percolation curve shows modified Darcy flow characteristics, while when the apparent fluidity up＂ non-Darcy percolation curve; 3） Oil-water is less than 1.0, the percolation curve is a ＂concave- two-phase relative permeability is affected by core permeability, fluid viscosity, apparent fluidity, and injection drive force; 4） The oil saturation of low- permeability sandstone reservoirs is mostly within 35%-60%, and the oil saturation also has a good positive correlation with the permeability and apparent fluidity.

关键词： Non-Darcy flow relative permeability oil saturation low-permeability sandstone

舰面飞机尾喷流对进气道温度场影响的仿真分析

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航空学报 2021年第8期42卷 181-191页

作者：王霄程健慧沈天荣许保成孟轩国防科技大学空天科学学院长沙410073 航空工业沈阳飞机设计研究所沈阳110035

舰载机在加力起飞时通过舰面安装的偏流板将高温尾喷流引向空中排出,避免了高温尾喷流损伤舰面工作人员和舰载设备,但一部分高温尾喷流与偏流板碰撞后的回流受发动机抽吸作用的影响,容易被进气道吸入,导致发动机推力降低,严重时诱发发... 详细信息

舰载机在加力起飞时通过舰面安装的偏流板将高温尾喷流引向空中排出,避免了高温尾喷流损伤舰面工作人员和舰载设备,但一部分高温尾喷流与偏流板碰撞后的回流受发动机抽吸作用的影响,容易被进气道吸入,导致发动机推力降低,严重时诱发发动机喘振,危害舰载机的使用安全。为了获得高温尾喷流与偏流板碰撞后的回流场流动机理以及参数影响规律,采用数值仿真分析方法开展了研究。首先,通过公开的试验数据验证了仿真分析方法的准确性;然后,完成了舰面环境下某型舰载机双发尾喷流冲击偏流板后的流动机理和温度场特征分析,获得了高温气体被进气道吸入的动态流动特性和进气道出口的温升率;最后,通过研究发动机转速不对称、来流风速、尾喷口到偏流板距离等参数对进气道出口温度畸变强度的影响规律,获得了尾喷口到偏流板的距离对回流场整体强度与分布起决定作用,以及进气口的位置影响进气道抽吸流场与回流场的耦合特性这一结论。

关键词：舰载机偏流板尾喷流温度畸变强度温升率

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赤霉素生物合成与信号传递对植物株高的调控

生物工程学报 2012年第2期28卷 144-153页

作者：魏灵珠程建徽李琳吴江浙江省农业科学院园艺研究所浙江杭州310021

植物株高是影响作物产量和品质的重要农艺性状。赤霉素(Gibberellins,GAs)是调控植物株高的重要激素,GA相关株高基因的克隆与分子机制研究对于合理调控作物生长发育和农业生产具有极其重要的利用价值,在水稻、小麦等粮食作物育种中得到... 详细信息

植物株高是影响作物产量和品质的重要农艺性状。赤霉素(Gibberellins,GAs)是调控植物株高的重要激素,GA相关株高基因的克隆与分子机制研究对于合理调控作物生长发育和农业生产具有极其重要的利用价值,在水稻、小麦等粮食作物育种中得到了广泛应用。为了促进GA在果树、花卉等园艺作物育种中的有效利用,文中在分子生物学水平上介绍GA生物合成和GA信号传递途径对植物株高的调控。

关键词：赤霉素株高生物合成信号传递

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中熟鲜食葡萄新品种天工蜜的选育

果树学报 2022年第2期39卷 318-320页

作者：程建徽魏灵珠向江吴江浙江省农业科学院园艺研究所杭州310021

天工蜜是由早甜×巨玫瑰杂交选育出的中熟欧美杂交种葡萄新品种。果穗呈圆锥形,具有较好的紧密度,全穗果粒成熟一致,果粒蓝黑色、椭圆形,果粉厚;平均果穗质量452.5 g,平均单粒质量8.7 g,最大粒质量12.5 g,果形指数1.1;每果粒含种子1~2粒... 详细信息

天工蜜是由早甜×巨玫瑰杂交选育出的中熟欧美杂交种葡萄新品种。果穗呈圆锥形,具有较好的紧密度,全穗果粒成熟一致,果粒蓝黑色、椭圆形,果粉厚;平均果穗质量452.5 g,平均单粒质量8.7 g,最大粒质量12.5 g,果形指数1.1;每果粒含种子1~2粒;果皮较易剥,果肉汁液多,质地适中,可溶性固形物含量(w,后同)为20.2%,可滴定酸含量为4.35 g·kg^(-1),维生素C含量87.0 mg·kg^(-1),草莓香型。果实生育期102 d,在浙江海宁大棚设施种植,果实成熟期8月上旬,该品种生长势较强,花芽分化优良,易结二次果,萌芽率78.2%,结果枝率达99.7%。抗病性较强,适宜在南方湿热气候长三角地区栽培。

关键词：鲜食葡萄新品种天工蜜中熟

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鲜食葡萄新品种‘天工翠玉’的选育

果树学报 2020年第6期37卷 945-947页

作者：程建徽魏灵珠向江吴江浙江省农业科学院园艺研究所杭州310021

‘天工翠玉’是以‘早甜’为母本‘,红富士’为父本,杂交选育出的欧美杂交种葡萄新品种。果穗圆锥形,果穗质量300~500 g;果粒椭圆形,平均粒质量8.3 g,果皮黄绿色、易剥,果梗与果粒分离难、不易落粒;果肉质地较软,汁液多、草莓香,可溶性... 详细信息

‘天工翠玉’是以‘早甜’为母本‘,红富士’为父本,杂交选育出的欧美杂交种葡萄新品种。果穗圆锥形,果穗质量300~500 g;果粒椭圆形,平均粒质量8.3 g,果皮黄绿色、易剥,果梗与果粒分离难、不易落粒;果肉质地较软,汁液多、草莓香,可溶性固形物含量20.5%,可滴定酸含量0.30%,维生素C含量54.1 mg·kg^-1。在浙北海宁地区设施种植8月上旬果实成熟。该品种生长势中庸,易成花、易坐果,具有较强的抗病性,栽培管理易。

关键词：鲜食葡萄新品种 ‘天工翠玉’

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无核葡萄新品种‘天工翡翠’的选育

果树学报 2019年第2期36卷 250-252页

作者：程建徽魏灵珠向江吴江浙江省农业科学院园艺研究所杭州310021

天工翡翠’是以'金手指’(二倍体)为母本'、鄞红’(四倍体)为父本,杂交选育出的欧美杂交种无核葡萄新品种。果穗圆柱形,平均穗质量496.1 g;果粒椭圆形,自然粒质量2.6～3.1 g,经赤霉素处理平均单粒质量为5.2 g,果皮黄绿色带粉红色晕;果... 详细信息

天工翡翠’是以'金手指’(二倍体)为母本'、鄞红’(四倍体)为父本,杂交选育出的欧美杂交种无核葡萄新品种。果穗圆柱形,平均穗质量496.1 g;果粒椭圆形,自然粒质量2.6～3.1 g,经赤霉素处理平均单粒质量为5.2 g,果皮黄绿色带粉红色晕;果肉质脆,淡哈密瓜香味,可溶性固形物含量(w,后同)为18.5%,可滴定酸含量为0.40%,维生素C含量为71.4 mg·kg^(-1),口感较好,无种子。在浙北海宁地区设施种植7月底果实成熟。该品种生长势强,易成花,具有较强的抗性。

关键词：无核葡萄新品种 ’天工翡翠’

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宝钢2RH冷凝水返水系统改造实践

宝钢技术 2017年第6期10卷 60-62页

作者：程建辉宝山钢铁股份有限公司炼钢厂上海200941

冷凝水系统是RH真空系统中的重要组成部分,原来的返水系统通过返送水泵来实现,既浪费电能,又要花费设备投资和维护费用。通过系统水力计算,提出了利用进水、回水的压差建立虹吸来返水,并在2RH进行实践。增加一套地面热井罐、阀门和管道... 详细信息

冷凝水系统是RH真空系统中的重要组成部分,原来的返水系统通过返送水泵来实现,既浪费电能,又要花费设备投资和维护费用。通过系统水力计算,提出了利用进水、回水的压差建立虹吸来返水,并在2RH进行实践。增加一套地面热井罐、阀门和管道,进而取消返送泵和相应的控制系统,这种重力式返水系统无需返送水泵,既节能又高效。

关键词：冷凝水 RH 虹吸