检索结果-南通市图书馆

Aquaporin-4 in the formation of cerebral edema following severe burns What role do arginine vasopressin levels play?

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2010年第7期5卷 531-537页

作者： Shifang Luo Shanquan Sun jianhua ran Kejian Wang Guiqiong He Department of Anatomy Chongqing Medical University Chongqing 400016 China Research Center of Neuroscience Chongqing Medical University Chongqing 400016 China

BACKGROUND： Aquaporin-4 （AQP-4）, which is able to rapidly transport water within the brain, is highly expressed in brain tissue. It also plays an important role in the formation of cerebral edema following brain injury. However, the role of AQP-4 in the formation of cerebral edema following severe bums remains unknown. OBJECTIVE： To study changes in AQP-4 protein and mRNA expression during formation of cerebral edema following severe burns, and to explore the correlation between AQP-4 protein and mRNA expression with plasma levels of arginine vasopressin （AVP）. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Research Center of Neuroscience, Chongqing Medical University from 2007 to 2008. MATERIALS： Biotin-labeled goat anti-rabbit antibody was provided by Beijing Zhongshan Biotechnology, China; in situ hybridization kit was provided by Wuhan Boster Biotechnology, China; rabbit anti-AQP-4 polyclonal antibody and horseradish peroxidase-labeled goat anti-rabbit IgG were provided by Chemicon, USA; AVP radioimmunoassay kit was provided by the Research Department of Neurobiology, the Second Military Medical University of Shanghai, China. METHODS： A total of 180 adult, healthy, Wistar rats were randomly assigned to control and burn groups with 30 rats in each group. The burn group was observed at five different time points： 2, 6, 12, 24, and 48 hours after burn. Hair on the mouse back was removed to expose skin on the back. After 1 day, skin with the hair removed was dipped into 100℃ water for 15 seconds to induce grade III bum injury that measures 30% of total bum surface area. MAIN OUTCOME MEASURES： Brain water content was measured using the dry-wet weight method. AQP-4 protein and mRNA expressions were detected using immunohistochemistry, in situ hybridization, Western blot, and reverse transcription-polymerase chain reaction; dynamic changes in plasma AVP were detected using radioimmunoassay. RESULTS： Brain water content gradua

关键词： burn injury cerebral edema aquaporin-4 aquaporin-4 mRNA arginine vasopressin brain injury

Cellular apoptosis and Caspase-12 expression in a rat model of progressive spinal cord compression

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2009年第8期4卷 570-576页

作者： Yijian Liang Kejian Wang Shanquan Sun Wei Li jianhua ran Guiqiong He Department of Human Anatomy College of Basic Medicine Chongqing Medical University Chongqing 400016 China Department of Orthopaedics Second People's Hospital of Chengdu Chengdu 610017 Sichuan Province China Institute of Neuroscience Chongqing Medical University Chongqing 400016 China

BACKGROUND： Studies have demonstrated that the mechanisms underlying cellular apoptosis signal transduction focus on two pathways： intracellular mitochondria and extracellular death receptor. The current evidence supports that signal transduction of cellular apoptosis also includes endoplasmic reticulum stress signal transduction. OBJECTIVE： To observe Caspase-12 expression and cellular apoptosis following ischemia in rats with progressive spinal cord compression, and to verify the influence of endoplasmic reticulum stress on the apoptosis induced by spinal cord injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal trial was performed at the Institute of Neuroscience in Chongqing Medical University between January and October in 2006. MATERIALS： Immunohistochemical kit, diaminobenzidine, and TUNEL kit were purchased from Beijing Zhongshan Biotechnology, China; rabbit anti-rat Caspase-12 monoclonal antibody was provided by Santa Cruz, USA. METHODS： Sixty Wistar rats, aged 3-4 months, were randomly assigned to a model group （n = 50）, which underwent spinal cord compression in the L1 segment following L1 laminectomy and articular process excision to establish a model of progressive spinal cord compression, and a sham-surgery group （n = 10）, which underwent only laminectomy. Starting with the first day after surgery, the rats were locally anesthetized, the skin was opened, and the screw was rotated by 1/4 of a cycle, twice weekly. MAIN OUTCOME MEASURES： At 3, 7, 14, 21, and 28 days after surgery, rats from each group were anesthetized, and the spinal cords were resected. Pathological changes following spinal cord compression were determined using hematoxylin-eosin staining, Nissl dye, and transmission electron microscopy. The TUNEL method was used to observe neuronal apoptosis in the compressed spinal cord segments. Immunohistochemistry and Western blot were utilized to detect Caspase-12 expression in the compressed segments. RESULTS： Cellular swelling, neu

关键词： progressive spinal cord compression apoptosis Caspase-12 rats endoplasmic reticulum stress

Discovery of novel diarylamides as orally active diuretics targeting urea transporters

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第1期11卷 181-202页

作者： Shun Zhang Yan Zhao Shuyuan Wang Min Li Yue Xu jianhua ran Xiaoqiang Geng Jinzhao He Jia Meng Guangying Shao Hong Zhou Zemei Ge Guangping Chen Runtao Li Baoxue Yang Department of Pharmacology School of Basic Medical SciencesPeking UniversityBeijing 100191China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100191China Key Laboratory of Molecular Cardiovascular Sciences Ministry of EducationBeijing 100191China School of Pharmaceutical Sciences Peking UniversityBeijing 100191China College of Pharmacy Inner Mongolia Medical UniversityHohhot 010110China Department of Anatomy and Neuroscience Center Chongqing Medical UniversityChongqing 400016China Department of Physiology Emory University School of MedicineAtlantaGA 30322USA

Urea transporters(UT)play a vital role in the mechanism of urine concentration and are recognized as novel targets for the development of salt-sparing ***,UT inhibitors are promising for development as novel *** the present study,a novel UT inhibitor with a diarylamide scaffold was discovered by high-throughput *** of the inhibitor led to the identifi-cation of a promising preclinical candidate,N-[4-(acetylamino)phenyl]-5-nitrofuran-2-carboxamide(1 H),with excellent in vitro UT inhibitory activity at the submicromolar *** half maximal inhibitory concentrations of 1 H against UT-B in mouse,rat,and human erythrocyte were 1.60,0.64,and0.13 mmol/L,*** investigation suggested that 8 mmol/L 1 H more powerfully inhibited UT-A1 at a rate of 86.8%than UT-B at a rate of 73.9%in MDCK cell *** interestingly,we found for the first time that oral administration of 1 H at a dose of 100 mg/kg showed superior diuretic effect in vivo without causing electrolyte imbalance in ***,1 H did not exhibit apparent toxicity in vivo and in vitro,and possessed favorable pharmacokinetic characteristics.1 H shows promise as a novel diuretic to treat hyponatremia accompanied with volume expansion and may cause few side effects.

关键词： Urea transporter inhibitor Diuretic Structure optimization Oral administration

High urea induces depression and LTP impairment through mTOR signaling suppression caused by carbamylation

在线全文

学校读者我要写书评

暂无评论

High urea induces depression and LTP impairment through mTOR...

羧基化聚苯乙烯荧光微球的合成及其在织物防伪中的应用

首届东方药理论坛暨2018年全国心脑血管药理学术会议、上海市药理学会第十九届学术年会

作者： Hongkai Wang Weiling Wang Boyue Huang jianhua ran Baoxue Yang Department of Pharmacology School of Basic Medical Sciencesand State Key Laboratory of Natural and Biomimetic DrugsPeking University

Background:Depression is one of the main factors lowering quality of life for patients with chronic physical *** is unknown whether a biological factor is implicated in ***,the end product of protein metabolism,has been considered to have negligible toxicity for a *** previous study showed depression phenotype in urea transporter (UT) B knockout mice,which suggests that

关键词： LTP High urea induces depression and LTP impairment through mTOR signaling suppression caused by carbamylation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

纺织学报 2023年第2期44卷 184-190页

作者：肖明黄亮罗龙永毕曙光冉建华武汉纺织大学生物质纤维与生态染整湖北省重点实验室湖北武汉430200

为解决防伪技术中荧光织物制备方法复杂和成本高的问题,采用分散聚合法制备表面带有负电荷的羧基化聚苯乙烯微球(CPS),再以阳离子表面活性剂十八烷基三甲基溴化铵为改性剂,利用静电自组装法吸附荧光染料异硫氰酸荧光素(FITC),制备羧基... 详细信息

为解决防伪技术中荧光织物制备方法复杂和成本高的问题,采用分散聚合法制备表面带有负电荷的羧基化聚苯乙烯微球(CPS),再以阳离子表面活性剂十八烷基三甲基溴化铵为改性剂,利用静电自组装法吸附荧光染料异硫氰酸荧光素(FITC),制备羧基化聚苯乙烯荧光微球(CPS-FITC),最后将其负载于织物上制备荧光织物。结果表明:所制备的荧光微球和荧光织物在紫外灯(365 nm)下具有明亮的黄绿色荧光,且放置不同时间(1~9 d)、在不同的pH值(3~11)下,经多次摩擦和水洗后均能保持良好的荧光稳定性。该制备方法操作简便、成本低且荧光强度高,可用于不同织物的防伪检测。

关键词：织物防伪羧基化聚苯乙烯微球异硫氰酸荧光素阳离子表面活性剂静电自组装荧光微球

尿素通道蛋白B敲除小鼠海马毛细血管的体视学

在线全文

学校读者我要写书评

暂无评论

解剖学杂志 2015年第4期38卷 442-445页

作者：侯良绢冉建华重庆三峡医药高等专科学校基础医学部解剖学教研室万州404120 重庆医科大学基础医学院解剖学教研室重庆400016 重庆医科大学基础医学院神经科学研究中心重庆400016

目的:研究尿素通道蛋白B(UT-B)敲除小鼠海马组织中尿素对海马毛细血管形态结构的影响,探讨UT-B基因敲除小鼠出现抑郁样行为的病理机制.方法:采用糖水偏好实验观察野生型和UT-B基因敲除小鼠的抑郁样行为,尿素检测试剂盒测定2型小鼠海马... 详细信息

目的:研究尿素通道蛋白B(UT-B)敲除小鼠海马组织中尿素对海马毛细血管形态结构的影响,探讨UT-B基因敲除小鼠出现抑郁样行为的病理机制.方法:采用糖水偏好实验观察野生型和UT-B基因敲除小鼠的抑郁样行为,尿素检测试剂盒测定2型小鼠海马尿素水平,免疫组织化学和体视学定量检测2型小鼠海马毛细血管总长度、总体积、总表面积、平均直径等形态学指标.结果:糖水偏好实验中,野生型和UT-B基因敲除小鼠的糖水偏爱指数分别为77.22％±3.93％vs6L 20％±4.42％,差异具有统计学意义.野生型和UT-B基因敲除小鼠海马尿素水平,海马毛细血管的总长度、总体积、总表面积、平均直径差异均有统计学意义.结论:UT-B敲除导致小鼠海马尿素堆积,引发小鼠海马毛细血管的形态改变,毛细血管总体积下降造成海马血灌注量不足而引发小鼠的抑郁样行为.

关键词：海马毛细血管尿素通道蛋白B 体视学小鼠

在线全文

学校读者我要写书评

暂无评论

数据链与飞机着陆系统的历史和前景

舰船电子工程 2009年第4期29卷 39-42页

作者：冉建华中国船舶重工集团公司第722研究所武汉430079

在介绍飞机着陆系统和数据链技术的历史与现状的基础上,探讨飞机着陆系统技术发展前景,论述数据链在其中的应用。

关键词：飞机着陆系统数据链新航行系统精密导航

在线全文

学校读者我要写书评

暂无评论

水通道蛋白4在大鼠眼球中的表达

解剖学报 2004年第6期35卷 656-659页

作者：冉建华孙善全重庆医科大学基础医学院解剖学教研室重庆400016

目的　从转录和翻译水平观察水通道蛋白 4 (AQP4 ) ,在正常Wistar大鼠眼球中的表达 ,为研究眼球中液体转运机制提供形态学基础。　方法　采用免疫组织化学SABC法和原位杂交技术。　结果　所测大鼠眼球呈较强的AQP4免疫反应阳性 ,角膜上... 详细信息

目的　从转录和翻译水平观察水通道蛋白 4 (AQP4 ) ,在正常Wistar大鼠眼球中的表达 ,为研究眼球中液体转运机制提供形态学基础。　方法　采用免疫组织化学SABC法和原位杂交技术。　结果　所测大鼠眼球呈较强的AQP4免疫反应阳性 ,角膜上皮细胞、虹膜色素上皮细胞、睫状体非色素上皮细胞、晶状体上皮细胞、晶状体纤维细胞、视网膜的内颗粒层、外颗粒层和节细胞层的细胞均为阳性细胞 ,免疫反应阳性物质主要分布在细胞膜上 ,胞核呈阴性。原位杂交结果同免疫组织化学染色基本一致 ,在以上细胞胞浆中均可检测到较强的AQP4mRNA阳性杂交信号 ,胞核呈阴性。　结论　AQP4可能在眼球中角膜和晶状体透明性的维持 ,房水的分泌和调节 ,视觉的激发和传递等功能活动中起着重要作用。

关键词：水通道蛋白4 免疫组织化学法原位杂交技术眼球大鼠

在线全文

学校读者我要写书评

暂无评论

壳聚糖改性织物中原位合成银纳米粒子

高等学校化学学报 2014年第2期35卷 415-420页

作者：彭俊军张馨吴毅明刘红玲冉建华李明杨锋武汉纺织大学化学与化工学院武汉430200

采用壳聚糖改性棉和涤纶织物,通过织物表面的壳聚糖原位吸附、还原银离子制备了纳米银抗菌织物。用场发射扫描电子显微镜（ SEM）、X射线能谱（ EDA）、X射线粉末衍射仪（ XRD）和反射光谱等对纳米银织物进行了表征,研究了银离子浓度和... 详细信息

采用壳聚糖改性棉和涤纶织物,通过织物表面的壳聚糖原位吸附、还原银离子制备了纳米银抗菌织物。用场发射扫描电子显微镜（ SEM）、X射线能谱（ EDA）、X射线粉末衍射仪（ XRD）和反射光谱等对纳米银织物进行了表征,研究了银离子浓度和壳聚糖浓度对纳米银织物的影响,并检测了纳米银织物的抗菌性能。结果表明,在无需任何还原剂的条件下,壳聚糖改性的棉和涤纶织物表面可以均匀地形成银纳米粒子,晶粒大小为5~10 nm,所制备的纳米银织物均具有优异的抗菌性能。

关键词：纳米银织物原位合成壳聚糖抗菌性能