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Protective mechanism of Coprinus comatus polysaccharide on acute alcoholic liver injury in mice,the metabolomics and gut microbiota investigation

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Food Science and Human Wellness 2024年第1期13卷 401-413页

作者： Jinyan Yu jianguang sun Min sun Weidong Li Dongmei Qi Yongqing Zhang Chunchao Han School of Pharmacy Shandong University of Traditional Chinese MedicineJinan 250355China Hepatology Department of Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan 250355China Experimental center Shandong University of Traditional Chinese MedicineJinan 250355China

Coprinus comatus polysaccharide(CCP)has significant hepatoprotective effect.To explore hepatoprotective mechanism of CCP,the study analyzed preventive effect of CCP on acute alcoholic liver injury in mice by histopathological examination and biochemical analysis.Simultaneously,hepatoprotective mechanism was also analyzed in conjunction with metabolomics and proliferation of gut microbiota.The results showed that CCP significantly decreased alanine aminotransferase(ALT),aspartate aminotransferase(AST)and triglyceride(TG)levels in serum of alcoholic liver disease(ALD)mice.Histopathological examination showed that CCP can significantly improve liver damage.Metabolomics results showed that there were significant differences in the level of metabolites in liver tissue of control group,ALD group and CCP group,including taurine,xanthosine,fumaric acid and arachidonic acid,among others.Metabolites pathways analysis showed that hepatoprotective effect of CCP was related to energy metabolism,biosynthesis of unsaturated fatty acids,amino acids metabolism and lipid metabolism.Additionally,CCP inhibited an increase in the number of Clostridium perfringens,Enterobacteriaceae and Enterococcus,and a decrease in the number of Lactobacillus and Bifidobacterium in the gut of ALD mice.All these findings suggested that CCP treatment reversed the phenotype of ethanol-induced liver injury and the associated metabolites pathways.

关键词： Coprinus comatus Polysaccharide Alcoholic liver disease Metabolomics Gut microbiota

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A hybrid method for product low-end disruptive innovation

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Frontiers of Mechanical Engineering 2022年第3期17卷 159-176页

作者： Yu WANG Runhua TAN Qingjin PENG jianguang sun Haoyu LI Fei YU School of Mechanical Engineering Hebei University of TechnologyTianjin300401China National Engineering Research Center for Technological Innovation Method and Tool Hebei University of TechnologyTianjin300401China Department of Mechanical Engineering University of ManitobaWinnipegMBR3T 2N2Canada

Product innovation is often a process for improving existing products.Low-end disruptive innovation(LDI)enables a product to meet the most price-sensitive customers in the low-end market.The existing LDI methods are mainly based on unnecessary characteristics of disruptive innovations.Thus,they cannot easily identify and respond to the LDI design needs.This study proposes a hybrid method for the product LDI in two levels of the product design based on the summarized definition and essential characteristics of LDI.Feasible areas of the product LDI are determined using a hybrid relational function model to identify the maturity of dominant technologies.The technologies are identified through the technical search and evaluation of the feasible area for innovation to form an initial LDI scheme.Then,the product function is optimized using the trimming concept of theory of inventive problem solving based on the characteristics of LDI.The final LDI scheme is formed and evaluated based on the essential characteristics of the product LDI.The feasibility of the proposed method is verified in the design of a new dropping pill machine.

关键词： low-end disruptive innovation product design design improvement theory of inventive problem solving TRIZ trimming

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芪术化积方治疗肝细胞癌癌前病变患者的随机对照研究

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中国全科医学 2024年第3期27卷 335-342,347页

作者：宁麟孙建光山东中医药大学第一临床医学院山东省济南市250014

背景肝细胞癌(HCC)存在发病率高、死亡率高的特点,在癌前病变阶段进行早期诊断及时干预可有效降低HCC的发生率,对其防治具有显著意义。现代医学对于HCC癌前病变的治疗存在干预时机难以把握、治疗手段单一等问题,中医药防治HCC癌前病变... 详细信息

背景肝细胞癌(HCC)存在发病率高、死亡率高的特点,在癌前病变阶段进行早期诊断及时干预可有效降低HCC的发生率,对其防治具有显著意义。现代医学对于HCC癌前病变的治疗存在干预时机难以把握、治疗手段单一等问题,中医药防治HCC癌前病变或许是一种简单有效的新途径。目的观察芪术化积方治疗HCC癌前病变(肝郁脾虚、痰瘀互结证)的临床疗效及安全性。方法纳入2019年10月—2020年3月在山东中医药大学附属医院、威海市中医院、泰安市中医院肝病科门诊就医且符合中医证型“肝郁脾虚、痰瘀互结证”的HCC癌前病变初诊患者为研究对象。收集患者的一般资料[性别、年龄、乙型病毒性肝炎(HBV)后肝硬化史、HCC家族史、10年以上饮酒史],患者的中医证候积分、肝功能指标[丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、白蛋白(ALB)、谷氨酰转肽酶(GGT)、碱性磷酸酶(ALP)]、肿瘤标志物指标[甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)、异常凝血酶原(DCP)]、影像学指标(病灶类型、病灶性质、病灶长径)、安全性指标、不良反应。采用随机数字表法将符合纳入标准且完成研究的64例患者分为观察组(n=34)和对照组(n=30),对照组予以抗病毒、抗炎保肝等常规治疗;观察组予以常规治疗联合芪术化积方治疗。疗程为48周,停药后随访48周,观察两组患者治疗周期及随访周期中HCC发生率及并发症的发生情况,比较两组患者治疗前后中医证候改善指标(中医证候积分、总有效率)、肝功能指标、肿瘤标志物指标、影像学指标(病灶长径、病灶稳定率、病灶改善率)、安全性指标(安全率)的差异。结果治疗后两组中医证候积分、ALT、AST、TBIL、GGT、ALP、AFP-L3均低于治疗前,ALB水平高于治疗前(P0.05)。随访周期结束后观察组HCC发生率(8.8%)与对照组(16.7%)比较,差异无统计学意义(P>0.05);并发症发生率(8.8%)低于对照组(30.0%)(P0.05)。结论芪术化积方治疗HCC癌前病变(肝郁脾虚、痰瘀互结证),在改善中医证候、改善肝功能、缩小病灶结节、降低远期HCC发生率及减少肝硬化并发症等方面有较好的临床疗效及安全性。

关键词：癌,肝细胞肝细胞癌癌前病变中医药疗法芪术化积方肝郁脾虚、痰瘀互结证随机对照试验临床观察

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Safety and feasibility of laparoscopic surgery for colorectal and gastric cancer under the Chinese multi-site practice policy:admittance standards of competence are needed

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Gastroenterology Report 2022年第1期10卷 490-497页

作者： Zhenghao Cai Haiqin Song Zhenfeng Huang Abraham Fingerhut Ximo Xu Hao Zhong Zhigang Li Yingjie Zhang Dachong Sha Dandan Bao Haibo Wang Binghua Cai Shangbo Hua Yanhui Zhang jianguang sun Ke Ye Jianwen Li Yong Lu Bo Feng Department of General Surgery Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghaiP.R.China Shanghai Minimally Invasive Surgery Center ShanghaiP.R.China Department of General Surgery People’s Hospital of Rui’anZhejiangP.R.China Section for Surgical Research Department of SurgeryMedical University of GrazGrazAustria Department of General Surgery Wuxi Huishan District People’s HospitalJiangsuP.R.China Department of General Surgery Zhoushan HospitalZhejiangP.R.China Department of General Surgery Cixi Third People’s HospitalZhejiangP.R.China Department of General Surgery People’s Hospital of WenzhouZhejiangP.R.China Department of General Surgery Chinese PLA 107 HospitalShandongP.R.China Department of General Surgery Rudong People’s HospitalJiangsuP.R.China Department of General Surgery Kunshan Hospital of Chinese MedicineJiangsuP.R.China Department of General Surgery Jingjiang Chinese Medicine HospitalJiangsuP.R.China Department of General Surgery Haiyan People’s HospitalZhejiangP.R.China Department of General Surgery Yueqing People’s HospitalZhejiangP.R.China Department of Medical Affairs Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghaiP.R.China

Background:The multi-site practice(MSP)policy has been practiced in China over 10 years.This study aimed to investigate the safety and feasibility of performing laparoscopic surgery for colorectal cancer(LSCRC)and gastric cancer(LSGC)under the Chinese MSP policy.Methods:We collected and analysed the data from 1,081 patients who underwent LSCRC or LSGC performed by one gastrointestinal surgeon in his original hospital(n=573)and his MSP institutions(n=508)between January 2017 and December 2020.Baseline demographics,intraoperative outcomes,post-operative recovery,and pathological results were compared between the original hospital and MSP institutions,as well as between MSP institutions with and without specific competence(surgical skill,operative instrument,perioperative multi-discipline team).Results:In our study,690 patients underwent LSCRC and 391 patients underwent LSGC.The prevalence of post-operative complications was comparable for LSCRC(11.5%vs 11.1%,P=0.89)or LSGC(15.2%vs 12.6%,P=0.46)between the original hospital and MSP institutions.However,patients in MSP institutions without qualified surgical assistant(s)and adequate instruments experienced longer operative time and greater intraoperative blood loss.The proportion of patients with inadequate lymph-node yield was significantly higher in MSP institutions than in the original hospital for both LSCRC(11.5%vs 21.2%,P<0.01)and LSGC(9.8%vs 20.5%,P<0.01).Conclusion:For an experienced gastrointestinal surgeon,performing LSCRC and LSGC outside his original hospital under the MSP policy is safe and feasible,but relies on the precondition that the MSP institutions are equipped with qualified surgical skills,adequate operative instruments,and complete perioperative management.

关键词： health policy gastrointestinal tumors laparoscopic surgery public health quality in health care

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Differential effects on apoptosis induction in hepatocyte lines by stable expression of hepatitis B virus X protein

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World Journal of Gastroenterology 2006年第29期12卷 4673-4682页

作者： Nicola Fiedler Ellen Quant Ludger Fink jianguang sun Ralph Schuster Wolfram H Gerlich Stephan Schaefer Institut for Medizinische Virologie Justus-LiebigUniversitat Frankfurter Str. 107 D-35392 GieBen Germany Abteilung fǖr Innere Medizin Christian AlbrechtUniversitat Kiel Germany Zentrum fǖr Innere Medizin Justus-LiebigUniversitat GieBen Germany Abteilung Virologie Universitat Rostock Schillingallee 70 18055 Rostock Germany Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 China

瞄准：肝炎 B 蛋白质 X (HBx ) 被显示了是弱 oncogenic 在试管内。HBx 的转变活动与肿瘤的功能压制的几的抑制或 p53 被连接了。我们学习了 HBx 是否可以在取决于房间类型的 p53 上有不同效果。方法：我们使用了人的肝细胞瘤房间线 Hep... 详细信息

瞄准：肝炎 B 蛋白质 X (HBx ) 被显示了是弱 oncogenic 在试管内。HBx 的转变活动与肿瘤的功能压制的几的抑制或 p53 被连接了。我们学习了 HBx 是否可以在取决于房间类型的 p53 上有不同效果。方法：我们使用了人的肝细胞瘤房间线 HepG2 和使不朽的鼠科的 hepatocyte 线 AML12 并且稳定地分析了表示了 HBx 的生理的数量的 transfected 克隆。P53 被紫外照耀导致。结果：由紫外照耀的 p53 正式就职由 HBx 的稳定的表示是未受影响的。然而，变得被 p53 激活的 cyclin 激酶禁止者 p21 (waf/cip/sdi ) 的表示在 HBx 被影响转变房间线 AML12-HBx9，然而并非在 HepG2。在 AML-HBx9，房间， p21 (waf/cip/sdi ) 蛋白质表示和 p21 (waf/cip/sdi ) 抄写是 deregulated。而且， apoptosis 的过程在调查的二根房间线以相反的方法被影响。当 HBx 的稳定的表示提高了紫外照耀在 HepG2 房间导致的 apoptosis 时， apoptosis 在 HBx 被减少转变 AML12-HBx9。P53 镇压了从 HBV enhancer 的抄写我，当由紫外照耀从表示向量或在内长的 p53 的正式就职以后表示了时。由内长的 p53 的压抑由在两根房间线的稳定地表示的 HBx 部分是可逆的。结论：HBx 的稳定的表示导致取决于使用的房间线的紫外照耀导致的 apoptosis 的解除管制。而在癌的表示导出 hepatocyte 线 HBx，在一根使不朽的 hepatocyte 线， HBx 扮演了 anti-apoptotic 提高的 apoptosis。

关键词：细胞凋亡乙型病毒肝炎治疗病理机制

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基于开放式校企合作平台的项目贯穿式教学模式研究

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廊坊师范学院学报（自然科学版） 2024年第1期24卷 100-105页

作者：于菲刘智光张鹏孙建广焦红磊河北工业大学天津300400 国家技术创新方法与实施工具工程技术研究中心天津300400 天津城建大学天津300384 河北科技师范学院河北秦皇岛066004

针对项目式教学实施过程中学生参与率低、与企业实践脱节、项目参与不深入等问题,构建符合项目式教学活动中各利益相关者需求的开放式校企合作平台和项目(需求)库。在此基础上,以项目为主线,将项目式教学贯穿至“创新通识课“”技术基... 详细信息

针对项目式教学实施过程中学生参与率低、与企业实践脱节、项目参与不深入等问题,构建符合项目式教学活动中各利益相关者需求的开放式校企合作平台和项目(需求)库。在此基础上,以项目为主线,将项目式教学贯穿至“创新通识课“”技术基础实践课“”创新方法专业课“”科技创新竞赛“”五位一体式项目研讨”及“毕业设计”等各教学环节,形成基于开放式校企合作平台的项目贯穿式教学模式,深化工程教育改革成效。

关键词：项目式教学贯穿式校企合作开放式创新

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Genotype-dependent activation or repression of HBV enhancer Ⅱ by transcription factor COUP-TF1

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World Journal of Gastroenterology 2006年第37期12卷 6054-6058页

作者： Silke F Fischer Katja Schmidt Nicola Fiedler Dieter Glebe Christian Schüttler jianguang sun Wolfram H Gerlich Reinald Repp Stephan Schaefer Instiut für Medizinische Virologie Justus-Liebig-Universitaet Giessen Germany Zentrum für Kinderheilkunde Justus-Liebig-Universitaet Giessen Klinik für Kinder-und Jugendmedizin Klinikum Fulda Germany Institut for Medizinische Mikrobiologie Immunologie und Hygiene Technische Universitaet München Germany Abteilung für Virologie Universitatt Rostock Germany

AIM: To study the expression of HBV enhancer Ⅱ by transcription factor COUP-TF1. METHODS: In order to study the regulation of HBV variants in the vicinity of the NRRE we cloned luciferase constructs containing the HBV enhancer Ⅱ from variants and from HBV genotypes A and D and cotransfected them together with expression vectors for COUP-TF1 into HepG2 cells. RESULTS: Our fi ndings show that enhancer Ⅱ of HBV genotype A is also repressed by COUP-TF1. In contrast, two different enhancer Ⅱ constructs of HBV genotype D were activated by COUP-TF1. The activation was independent of the NRRE because a natural variant with a deletion of nt 1763-1770 was still activated by COUP- TF1. CONCLUSION: Regulation of transcription of the HBV genome seems to differ among HBV genomes derived from different genotypes. These differences in transcriptional control among HBV genotypes may be the molecular basis for differences in the clinical course among HBV genotypes.

关键词：基因表达乙型病毒肝炎治疗病毒感染

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The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (Linum usitatissimum L.)

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Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei Xie Jing Li Wan Li Lijun sun Zhigang Dai Wenzhi Zhou jianguang Su Jian sun School of Life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu Biotechnology Co.Ltd. Nantong 226001China

Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction

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SYSTEMATIC METHOD TO GENERATE NEW IDEAS IN FUZZY FRONT END USING TRIZ

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Chinese Journal of Mechanical Engineering 2008年第2期21卷 114-119页

作者： TAN Runhua MA Lihui YANG Bojun sun jianguang Institute of Design for Innovation Hebei University of Technology Tianjin 300130 China

The obstacle for idea generation in fuzzy front end （FFE） is difficult to apply knowledge in different fields for designers. Theory of inventive problem solving TRIZ and computer-aided innovation systems （CAIs） which are TRIZ-base software systems with a knowledge base provide a framework for knowledge application in different fields. The major methods in TRIZ are selected, which have four types. The problems to be solved for each method are summarized and mapping from the problems to the methods is given. Systematic method with eight paths to integrate the methods and problems is formed. A case study shows the idea generation in FFE using the integrated method step by step.

关键词： Idea generation Fuzzy front end （FFE） Systematic method TRIZ

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氨基葡萄糖联合膝关节腔注射富血小板血浆治疗膝骨关节炎的临床效果

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中国医药 2024年第3期19卷 420-425页

作者：林伟明廖红兴孙剑光梁传兴黄略李惠民李灏广东省梅州市人民医院关节外科广东省梅州市医学科学院梅州514031 广东医科大学第一临床医学院湛江523808

目的探讨氨基葡萄糖联合膝关节腔注射富血小板血浆(PRP)治疗膝骨关节炎(KOA)的临床效果。方法选取2018年1月至2022年3月于广东省梅州市人民医院关节外科就诊的81例KOA患者,按随机数字表法分为观察组(40例)和对照组(41例)。对照组予硫酸... 详细信息

目的探讨氨基葡萄糖联合膝关节腔注射富血小板血浆(PRP)治疗膝骨关节炎(KOA)的临床效果。方法选取2018年1月至2022年3月于广东省梅州市人民医院关节外科就诊的81例KOA患者,按随机数字表法分为观察组(40例)和对照组(41例)。对照组予硫酸氨基葡萄糖胶囊治疗,观察组在对照组基础上联合膝关节腔注射富血小板血浆治疗,2组均连续治疗12个月。比较2组临床疗效以及视觉模拟量表(VAS)评分、美国特种外科医院膝关节评分(HSS)、血液标本指标、关节积液指标水平以及不良反应发生率。结果观察组总有效率高于对照组[95.0%(38/40)比73.2%(30/41)](χ^(2)=7.161,P=0.008)。治疗后6、12个月,2组VAS评分均低于治疗前、且观察组低于对照组,2组HSS评分均高于治疗前、且观察组高于对照组(均P0.05)。结论氨基葡萄糖联合膝关节腔注射PRP治疗KOA的临床效果良好,可明显改善患者膝关节功能、减轻患者炎症反应。

关键词：膝骨关节炎氨基葡萄糖关节腔注射富血小板血浆

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