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Neural Regeneration Research 2011年第17期6卷 1291-1295页

作者： Gang Chen jiangbei cao Weidong Mi Anesthesia and Operation Center PLA General Hospital Beijing 100853 China

We induced ischemic brain injury in aging rats to examine the effects of varying doses of propofol on hippocampal activities of acetylcholinesterase, N-methyI-D-aspartate receptors, and y-aminobutyric acid receptors. Propofol exhibited no obvious impact on acetylcholinesterase activity, but directly activated the y-aminobutyric acid receptor. The neuroprotective function of propofol on the hippocampus of aging rats following cerebral ischemic injury may be related to altered activities of y-aminobutyric acid receptors and N-methyI-D-aspartate receptors.

关键词： propofol brain injury acetylcholinesterase y-aminobutyric acid receptor N-methyi-D-asparLate receptor aging rat

Effect of propofol on brain-derived neurotrophic factor and tyrosine kinase receptor B in the hippocampus of aged rats with chronic cerebral ischemia

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Neural Regeneration Research 2012年第21期7卷 1645-1649页

作者： Gang Chen Qiang Fu jiangbei cao Weidong Mi Anesthesia and Operation Center PLA General Hospital Beijing 100853 China

We intraperitoneally injected 10 and 50 mg/kg of propofol for 7 consecutive days to treat a rat model of chronic cerebral ischemia. A low-dose of propofol promoted the expression of brain-derived neurotrophic factor, tyrosine kinase receptor B, phosphorylated cAMP response element binding protein, and cAMP in the hippocampus of aged rats with chronic cerebral ischemia, but a high-dose of propofol inhibited their expression. Results indicated that the protective effect of propofol against cerebral ischemia in aged rats is related to changes in the expression of brain-derived neurotrophic factor and tyrosine kinase receptor B in the hippocampus, and that the cAMP-cAMP responsive element binding protein pathway is involved in the regulatory effect of propofol on brain-derived neurotrophic factor expression.

关键词： propofol chronic cerebral ischemia aged brain-derived neurotrophic factor tyrosine kinasereceptor B cAMP-cAMP responsive element binding protein neural regeneration

Propofol increases in vitro proliferation of cultured rat hippocampal precursor cells inhibited by corticosterone

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Neural Regeneration Research 2009年第12期4卷 1088-1092页

作者： jiangbei cao Weidong Mi Yunfeng Li Hongxia Chen Anesthesia and Operation Center the General Hospital of the People's Liberation Army Beijing 100853 China Beijing Institute of Pharmacology and Toxicology Beijing 100850 China

BACKGROUND： Previous studies have shown that propofol enhances proliferation of cultured hippocampal precursor cells in vitro and increases proliferation of cultured hippocampal precursor cells inhibited by corticosterone. Because gamma-aminobutyric acid A （GABA-A） receptor is the functional target for propofol, the proliferative effects of propofol are thought to take place through GABA-A receptor. OBJECTIVE： To determine whether propofol enhances proliferation of rat hippocampal precursor cells inhibited by corticosterone by upregulating expression of GABA-A receptor. DESIGN, TIME AND SETTING： A comparative, observational, in vitro experiment was performed at the Beijing Institute of Pharmacology and Toxicology from April 2005 to April 2006. MATERIALS： Propofol was purchased from AstraZeneca, italy; corticosterone was purchased from Sigma, USA; bicuculline was purchased from Alexis, Switzerland. METHODS： Hippocampal precursor cells were isolated from newborn Wistar rats and cultured in vitro. The second passage of precursor cells was grouped according to the various drugs added to the culture medium： 0.5 μmol/L propofol; 2.5 pmol/L propofol; 100 μmol/L corticosterone; 10 μmol/L bicuculline; 100 μmol/L corticosterone and 0.5 μmol/L propofol; 100 μmol/L corticosterone and 2.5 μmol/L propofol; 100 μmol/L corticosterone, 10 μmol/L bicuculline, and 0.5 μmol/L propofol; 100 μmol/L corticosterone, 10 μmol/L bicuculline, and 2.5 μmol/L propofol; 100 μmol/L corticosterone and 10 pmol/L bicuculline. The cells were cultured for 24 hours with medium containing the respective concentration of drug. The control group consisted of precursor cells absent of drug treatment. MAIN OUTCOME MEASURES： The MTT and ^3H-TdR incorporation assays were used to detect proliferative effects of propofol and bicuculline on cultured rat hippocampal precursor cells inhibited by corticosterone. Immunocytochemistry was used to detect GABA-A receptor expression. Enzyme-linked irnmunosorbent assay was

关键词： propofol corticosterone hippocampal receptor, gamma-aminobutyric acid A precursor cells

Dopaminergic Neurons in the Ventral Tegmental–Prelimbic Pathway Promote the Emergence of Rats from Sevoflurane Anesthesia

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Neuroscience Bulletin 2022年第4期38卷 417-428页

作者： Yanping Song Ruitong Chu Fuyang cao Yanfeng Wang Yanhong Liu jiangbei cao Yongxin Guo Weidong Mi Li Tong Anesthesia and Operation Center The First Medical Center of ChinesePLA General HospitalBeijing 100853China Department of Anesthesia Second Affiliated Hospital of Naval Medical UniversityShanghai 200003China Chinese PLA Medical School Beijing 100853China Department of Anesthesia The Sixth Medical Center of ChinesePLA General HospitalBeijing 100048China Department of Anesthesia Xiangya HospitalCentral South UniversityChangsha 410008China

Dopaminergic neurons in the ventral tegmental area(VTA)play an important role in cognition,emergence from anesthesia,reward,and aversion,and their projection to the cortex is a crucial part of the"bottom-up"ascending activating *** prelimbic cortex(PrL)is one of the important projection regions of the ***,the roles of dopaminergic neurons in the VTA and the VTADA–PrL pathway under sevoflurane anesthesia in rats remain *** this study,we found that intraperitoneal injection and local microinjection of a dopamine D1 receptor agonist(Chloro-APB)into the PrL had an emergence-promoting effect on sevoflurane anesthesia in rats,while injection of a dopamine D1 receptor antagonist(SCH23390)deepened *** results of chemogenetics combined with microinjection and optogenetics showed that activating the VTADA–PrL pathway prolonged the induction time and shortened the emergence time of *** results demonstrate that the dopaminergic system in the VTA has an emergence-promoting effect and that the bottom-up VTADA–PrL pathway facilitates emergence from sevoflurane anesthesia.

关键词： Ventral tegmental area Prelimbic cortex Dopamine Sevoflurane General anesthesia

Efficacy and Safety of Dexmedetomidine during Anesthesia Induction of Patients with Intracranial Tumor: A Preliminary Observational Trial

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Pharmacology & Pharmacy 2013年第8期4卷 584-589页

作者： jiangbei cao Wenzhu Shi Weidong Mi Hong Zhang Department of Anesthesiology and Operation Center PLA General Hospital Beijing China Department of Anesthesiology and Operation Center PLA General Hospital Beijing China.

Background: The efficacy and safety of dexmedetomidine during the anesthesia induction of intracranial tumor patients remain unknown. We wondered whether loading infusion of dexmedetomidine 1 μg/kg over 10 min to intracranial tumor patients was as efficient and safe as to those abdominal disease patients. Methods: Patients aged 18-60 years, male or female, ASA I or II, scheduled for intracranial tumor resection (Group N, n = 30) or abdominal operation (Group A, n = 30) were enrolled in this observational trial. Dexmedetomidine was administrated with a loading dosage of 1 μg/kg over 10 min following with continuous infusing of 0.5 μg/kg/h. Fentanyl, propofol and rocuronium were sequentially administered for anesthesia induction. Heart rate (HR), blood pressure (BP), pulse oxygen saturation (SpO2), bispectral index (BIS) and other adverse effects were recorded from the beginning of loading infusion of dexmedetomidine to the end of endotracheal intubation. Results: Among with loading infusion, HR and BIS value decreased and were significantly lower at the end of infusion than before infusion (P P > 0.05). One patient of Group N dropped out from this trial because of a serious headache. 14 of 29 patients during dexmedetomidine loading infusion suffered hypoxemia (SpO2 P Conclusion: A loading dosage of 1 μg/kg of dexmedetomidine was not suitable for the anesthesia induction of intracranial tumor patients as compared to patients undergoing abdominal operation.

关键词： Dexmedetomidine Intracranial Tumor Adverse Effects Loading Infusion

麻醉机制研究又一利器:高分辨光场智能成像显微平台

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中华麻醉学杂志 2022年第10期42卷 1153-1155页

作者：曹江北米卫东解放军总医院第一医学中心麻醉科北京100853

在过去的一个世纪中,神经科学家主要依靠电生理记录技术监测活体动物的神经活动状态。膜片钳和神经微电极技术是最常用的电生理技术:膜片钳可适用于单个微观神经细胞之兴奋电活动的检测[1];而传统的神经微电极可以记录众多神经元电活动... 详细信息

在过去的一个世纪中,神经科学家主要依靠电生理记录技术监测活体动物的神经活动状态。膜片钳和神经微电极技术是最常用的电生理技术:膜片钳可适用于单个微观神经细胞之兴奋电活动的检测[1];而传统的神经微电极可以记录众多神经元电活动形成的局部场电位,主要缺点为空间分辨率低。随着神经像素技术的出现,神经微电极记录的分辨率水平被提高到单细胞水平,允许同时记录大脑中上百个神经元的活动状态[2,3],对理解神经网络的工作机制提供了有力的技术支撑。

关键词：活动状态空间分辨率神经网络神经科学家神经微电极膜片钳电生理技术技术监测

右美托咪定对大鼠局灶性脑缺血-再灌注损伤后抗氧化能力的影响

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临床麻醉学杂志 2016年第5期32卷 488-490页

作者：兰琛曹江北中国人民解放军总医院麻醉手术中心 100853

目的观察右美托咪定预先处理对大鼠局灶性脑缺血-再灌注损伤后抗氧化能力的影响。方法健康雄性SD大鼠42只,体重250~280g,随机分为假手术组（Sham组）、缺血-再灌注组（IR组）和右美托咪定组（DEX组）,每组14只。采用大脑中动脉线栓法制... 详细信息

目的观察右美托咪定预先处理对大鼠局灶性脑缺血-再灌注损伤后抗氧化能力的影响。方法健康雄性SD大鼠42只,体重250~280g,随机分为假手术组（Sham组）、缺血-再灌注组（IR组）和右美托咪定组（DEX组）,每组14只。采用大脑中动脉线栓法制作大鼠局灶性脑缺血-再灌注损伤模型,具体方法为线栓法栓塞大脑中动脉2h后恢复再灌注。Sham组仅分离血管,不留置线栓;于大脑中动脉栓塞前30 min DEX组给予腹腔注射盐酸右美托咪定注射液100μg/kg（4μg/ml）,IR组腹腔注射等量的生理盐水。三组均于再灌注24h后取8只大鼠进行神经功能评分,测定脑梗死体积;剩余6只大鼠取脑组织检测超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPX）、谷胱甘肽还原酶（GR）和过氧化氢酶（CAT）活性。结果 IR组和DEX组神经功能评分明显高于Sham组,脑梗死体积明显大于Sham组,SOD、GSH-PX、GR、CAT活性明显低于Sham组（P〈0.05）;DEX组大鼠神经功能评分明显低于IR组,脑梗死体积明显小于IR组,SOD、GSH-PX、GR、CAT活性明显高于IR组（P〈0.05）。结论右美托咪定有保护内源性抗氧化酶活性作用,可能有助于减轻大鼠脑缺血-再灌注损伤。

关键词：右美托咪定再灌注损伤抗氧化酶脑

不同每搏量变异度指导下的目标导向液体治疗对老年患者术后认知功能的影响

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临床麻醉学杂志 2016年第7期32卷 629-632页

作者：吕雪彩曹江北潘伟米卫东解放军总医院麻醉手术中心北京市100853

目的探讨每搏量变异度（stroke volume variation,SVV）指导的目标导向液体治疗对单膝关节置换术老年患者术后认知功能的影响。方法择期单膝关节置换术老年患者100例,随机均分为A组和B组。A组维持术中SVV 4%-9%,B组维持术中SVV 10%-14%... 详细信息

目的探讨每搏量变异度（stroke volume variation,SVV）指导的目标导向液体治疗对单膝关节置换术老年患者术后认知功能的影响。方法择期单膝关节置换术老年患者100例,随机均分为A组和B组。A组维持术中SVV 4%-9%,B组维持术中SVV 10%-14%。记录患者术中及术后24h液体出入量,术前及术后第1、3、5天C反应蛋白（C reactive protein,CRP）、白细胞介素-6（interleukin-6,IL-6）,记录患者松止血带相关低血压和术前、术后第5天POCD的发生情况。结果两组患者性别、年龄等一般情况差异无统计学意义;与B组比较,A组术中输液量及术后24h尿量明显增加（P〈0.05）;与术前比较,两组患者术后炎性因子含量均明显升高（P〈0.05）;B组术后CRP和IL-6明显高于A组（P〈0.05）。A组因松止血带相关低血压发生率为22例（44%）,明显低于B组的43例（86%）（P〈0.05）。POCD的发生率A组为11例（22.0%）,B组为5例（10.0%）,差异无统计学意义。结论 SVV指导术中液体治疗可以降低老年单膝关节置换术患者松止血带相关的低血压发生率以及围术期炎性反应。

关键词：每搏量变异度单膝关节置换术术后认知功能障碍目标导向液体治疗

激活小鼠ZI区GABA能神经元可促进七氟醚和丙泊酚的麻醉诱导而对麻醉维持及觉醒无影响

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南方医科大学学报 2023年第5期43卷 718-726页

作者：曹福羊郭永馨郭舒婷周志康曹江北仝黎米卫东解放军总医院第一医学中心麻醉科北京100853 解放军总医院第六医学中心麻醉科北京100048

目的探索未定带区(ZI)γ-氨基丁酸(GABA)能神经元对七氟醚和丙泊酚麻醉-觉醒的调控作用。方法将48只雄性C57BL/6J小鼠分为8组(6只/组)。研究七氟醚麻醉时,化学遗传学实验分为hM3Dq组(注射携带hM3Dq的腺相关病毒)和mCherry组(注射仅携带m... 详细信息

目的探索未定带区(ZI)γ-氨基丁酸(GABA)能神经元对七氟醚和丙泊酚麻醉-觉醒的调控作用。方法将48只雄性C57BL/6J小鼠分为8组(6只/组)。研究七氟醚麻醉时,化学遗传学实验分为hM3Dq组(注射携带hM3Dq的腺相关病毒)和mCherry组(注射仅携带mCherry的对照病毒);光遗传学实验分为ChR2组(注射携带ChR2的腺相关病毒)和GFP组(注射仅携带GFP的对照病毒),注射剂量均为100 nL。研究丙泊酚麻醉时,化学遗传学实验分为hM3Dq组和mCherry组;光遗传学实验分为ChR2组和GFP组。上述各组处理方法同七氟醚。通过化学遗传学和光遗传学技术激活ZI区GABA能神经元,观察其对七氟醚和丙泊酚的麻醉诱导及觉醒的调节作用,通过脑电监测观察其在七氟醚麻醉维持中的作用。结果在七氟醚麻醉中,与mCherry组比,hM3Dq组麻醉诱导时间显著缩短(P<0.05),与GFP组比,ChR2组麻醉诱导时间显著缩短(P<0.01),而两实验中麻醉觉醒时间差异均无统计学意义。在丙泊酚麻醉中,hM3Dq组比mCherry组麻醉诱导时间显著缩短(P<0.05),ChR2组比GFP组麻醉诱导时间显著缩短(P<0.01),而两实验中麻醉觉醒时间差异均无统计学意义。光遗传激活ZI区GABA能神经元没有改变七氟醚麻醉维持时的脑电频谱。结论ZI区GABA能神经元能够促进七氟醚和丙泊酚的麻醉诱导,而对麻醉维持及觉醒无调节作用。

关键词：七氟醚丙泊酚丘脑未定带 γ-氨基丁酸 GABA能神经元

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老年患者术后谵妄的研究进展

中华老年多器官疾病杂志 2023年第10期22卷 783-786页

作者：罗云根曹江北中国人民解放军总医院第一医学中心麻醉科北京100853 解放军医学院北京100853

术后谵妄(POD)是手术后一种常见的急性中枢神经系统综合征。由于生理机能的减退,老年人较儿童和成年人更易发生POD,且POD严重影响着老年患者的术后转归。随着社会的老龄化,老年患者手术量每年递增,POD的临床研究已成为麻醉学领域的热点... 详细信息

术后谵妄(POD)是手术后一种常见的急性中枢神经系统综合征。由于生理机能的减退,老年人较儿童和成年人更易发生POD,且POD严重影响着老年患者的术后转归。随着社会的老龄化,老年患者手术量每年递增,POD的临床研究已成为麻醉学领域的热点问题之一。本文从风险预测、预后评估、预防策略、治疗方案4个方向对POD的临床研究进展进行综述,以期为老年POD的临床防治工作提供依据。

关键词：老年人术后谵妄预后